Elucidating the role of ferrous ion cocatalyst in enhancing dilute acid pretreatment of lignocellulosic biomass.

Abstract:

BACKGROUND:Recently developed iron cocatalyst enhancement of dilute acid pretreatment of biomass is a promising approach for enhancing sugar release from recalcitrant lignocellulosic biomass. However, very little is known about the underlying mechanisms of this enhancement. In the current study, our aim was to identify several essential factors that contribute to ferrous ion-enhanced efficiency during dilute acid pretreatment of biomass and to initiate the investigation of the mechanisms that result in this enhancement. RESULTS:During dilute acid and ferrous ion cocatalyst pretreatments, we observed concomitant increases in solubilized sugars in the hydrolysate and reducing sugars in the (insoluble) biomass residues. We also observed enhancements in sugar release during subsequent enzymatic saccharification of iron cocatalyst-pretreated biomass. Fourier transform Raman spectroscopy showed that major peaks representing the C-O-C and C-H bonds in cellulose are significantly attenuated by iron cocatalyst pretreatment. Imaging using Prussian blue staining indicated that Fe2+ ions associate with both cellulose/xylan and lignin in untreated as well as dilute acid/Fe2+ ion-pretreated corn stover samples. Analyses by scanning electron microscopy and transmission electron microscopy revealed structural details of biomass after dilute acid/Fe2+ ion pretreatment, in which delamination and fibrillation of the cell wall were observed. CONCLUSIONS:By using this multimodal approach, we have revealed that (1) acid-ferrous ion-assisted pretreatment increases solubilization and enzymatic digestion of both cellulose and xylan to monomers and (2) this pretreatment likely targets multiple chemistries in plant cell wall polymer networks, including those represented by the C-O-C and C-H bonds in cellulose.

journal_name

Biotechnol Biofuels

authors

Wei H,Donohoe BS,Vinzant TB,Ciesielski PN,Wang W,Gedvilas LM,Zeng Y,Johnson DK,Ding SY,Himmel ME,Tucker MP

doi

10.1186/1754-6834-4-48

subject

Has Abstract

pub_date

2011-11-10 00:00:00

pages

48

issn

1754-6834

pii

1754-6834-4-48

journal_volume

4

pub_type

杂志文章
  • Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations.

    abstract:BACKGROUND:Reduced yields of ethanol due to bacterial contamination in fermentation cultures weaken the economics of biofuel production. Lactic acid bacteria are considered the most problematic, and surveys of commercial fuel ethanol facilities have found that species of Lactobacillus are predominant. Bacteriophage lyt...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-20

    authors: Roach DR,Khatibi PA,Bischoff KM,Hughes SR,Donovan DM

    更新日期:2013-02-07 00:00:00

  • Proteomic and metabolomic analysis of the cellular biomarkers related to inhibitors tolerance in Zymomonas mobilis ZM4.

    abstract:Background:Toxic compounds present in both the hydrolysate and pyrolysate of lignocellulosic biomass severely hinder the further conversion of lignocellulose-derived fermentable sugars into useful chemicals by common biocatalysts like Zymomonas mobilis, which has remarkable advantages over yeast. Although the extra det...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1287-5

    authors: Chang D,Yu Z,Ul Islam Z,French WT,Zhang Y,Zhang H

    更新日期:2018-10-16 00:00:00

  • Thermophilic fermentation of acetoin and 2,3-butanediol by a novel Geobacillus strain.

    abstract:UNLABELLED: BACKGROUND:Acetoin and 2,3-butanediol are two important biorefinery platform chemicals. They are currently fermented below 40°C using mesophilic strains, but the processes often suffer from bacterial contamination. RESULTS:This work reports the isolation and identification of a novel aerobic Geobacillus s...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-5-88

    authors: Xiao Z,Wang X,Huang Y,Huo F,Zhu X,Xi L,Lu JR

    更新日期:2012-12-06 00:00:00

  • Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions.

    abstract:BACKGROUND:Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. RESULTS:In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculu...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0286-z

    authors: Rakicka M,Lazar Z,Dulermo T,Fickers P,Nicaud JM

    更新日期:2015-07-25 00:00:00

  • Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila.

    abstract:BACKGROUND:Brown algae are promising feedstocks for biofuel production with inherent advantages of no structural lignin, high growth rate, and no competition for land and fresh water. However, it is difficult for one microorganism to convert all components of brown algae with different oxidoreduction potentials to etha...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0494-1

    authors: Ji SQ,Wang B,Lu M,Li FL

    更新日期:2016-04-01 00:00:00

  • Metabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle.

    abstract:Background:S-Adenosylmethionine (SAM) is a critical cofactor involved in many biochemical reactions. However, the low fermentation titer of SAM in methionine-free medium hampers commercial-scale production. The SAM synthesis pathway is specially related to the tricarboxylic acid (TCA) cycle in Bacillus amyloliquefacien...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1554-0

    authors: Ruan L,Li L,Zou D,Jiang C,Wen Z,Chen S,Deng Y,Wei X

    更新日期:2019-09-09 00:00:00

  • Xylan epitope profiling: an enhanced approach to study organ development-dependent changes in xylan structure, biosynthesis, and deposition in plant cell walls.

    abstract:Background:Xylan is a major hemicellulosic component in the cell walls of higher plants especially in the secondary walls of vascular cells which are playing important roles in physiological processes and overall mechanical strength. Being the second most abundant cell wall polymer after cellulose, xylan is an abundant...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0935-5

    authors: Peralta AG,Venkatachalam S,Stone SC,Pattathil S

    更新日期:2017-11-30 00:00:00

  • Nitrogen and phosphorus removal from anaerobically digested wastewater by microalgae cultured in a novel membrane photobioreactor.

    abstract:Background:With the further development of anaerobic digestion, an increasing output of anaerobically digested wastewater (ADW), which typically contained high concentrations of ammonium, phosphate, and suspended solids, was inevitable. Microalgae cultivation offered a potential waste-to-value strategy to reduce the hi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1190-0

    authors: Chen X,Li Z,He N,Zheng Y,Li H,Wang H,Wang Y,Lu Y,Li Q,Peng Y

    更新日期:2018-07-09 00:00:00

  • Freshwater microalgae harvested via flocculation induced by pH decrease.

    abstract:BACKGROUND:Recent studies have demonstrated that microalga has been widely regarded as one of the most promising raw materials of biofuels. However, lack of an economical, efficient and convenient method to harvest microalgae is a bottleneck to boost their full-scale application. Many methods of harvesting microalgae, ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-98

    authors: Liu J,Zhu Y,Tao Y,Zhang Y,Li A,Li T,Sang M,Zhang C

    更新日期:2013-07-09 00:00:00

  • RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa.

    abstract:BACKGROUND:Low- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental glucose fluctuation for their growth and competitiveness. In Neurospora crassa, the dual-affinity glucose transport system consists of a low-affinity glucose transporter GLT-1 and two high-affi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-021-01877-2

    authors: Li J,Liu Q,Li J,Lin L,Li X,Zhang Y,Tian C

    更新日期:2021-01-28 00:00:00

  • Deletion of meso-2,3-butanediol dehydrogenase gene budC for enhanced D-2,3-butanediol production in Bacillus licheniformis.

    abstract:BACKGROUND:D-2,3-butanediol has many industrial applications such as chiral reagents, solvents, anti-freeze agents, and low freezing point fuels. Traditional D-2,3-butanediol producing microorganisms, such as Klebsiella pneumonia and K. xoytoca, are pathogenic and not capable of producing D-2,3-butanediol at high optic...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-16

    authors: Qi G,Kang Y,Li L,Xiao A,Zhang S,Wen Z,Xu D,Chen S

    更新日期:2014-01-29 00:00:00

  • Remembering Mary (1917 to 2008): editorial introduction to the thematic series on the life and lifework of Mary Mandels, first lady of cellulase research.

    abstract::Editorial introduction to the thematic series on the life and lifework of Mary Mandels. ...

    journal_title:Biotechnology for biofuels

    pub_type: 社论

    doi:10.1186/1754-6834-2-23

    authors: Bayer EA

    更新日期:2009-09-01 00:00:00

  • Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate.

    abstract:BACKGROUND:Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to 'hydrolysate toxicity,' a maj...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0233-z

    authors: Mohagheghi A,Linger JG,Yang S,Smith H,Dowe N,Zhang M,Pienkos PT

    更新日期:2015-03-31 00:00:00

  • SSF of steam-pretreated wheat straw with the addition of saccharified or fermented wheat meal in integrated bioethanol production.

    abstract:BACKGROUND:Integration of second-generation (2G) bioethanol production with existing first-generation (1G) production may facilitate commercial production of ethanol from cellulosic material. Since 2G hydrolysates have a low sugar concentration and 1G streams often have to be diluted prior to fermentation, mixing of st...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-169

    authors: Erdei B,Hancz D,Galbe M,Zacchi G

    更新日期:2013-11-29 00:00:00

  • Periodic-peristole agitation for process enhancement of butanol fermentation.

    abstract:BACKGROUND:Mass transfer plays an important role in determining the efficiency of the biofuel conversion. However, adverse effect of shear stress from traditional agitation inhibits the cell growth and production of biofuels. How to enhance the mass transfer with less adverse effect is considered as one of the importan...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0409-6

    authors: Xia ML,Wang L,Yang ZX,Chen HZ

    更新日期:2015-12-23 00:00:00

  • Deep metagenome and metatranscriptome analyses of microbial communities affiliated with an industrial biogas fermenter, a cow rumen, and elephant feces reveal major differences in carbohydrate hydrolysis strategies.

    abstract:BACKGROUND:The diverse microbial communities in agricultural biogas fermenters are assumed to be well adapted for the anaerobic transformation of plant biomass to methane. Compared to natural systems, biogas reactors are limited in their hydrolytic potential. The reasons for this are not understood. RESULTS:In this pa...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0534-x

    authors: Güllert S,Fischer MA,Turaev D,Noebauer B,Ilmberger N,Wemheuer B,Alawi M,Rattei T,Daniel R,Schmitz RA,Grundhoff A,Streit WR

    更新日期:2016-06-07 00:00:00

  • Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity.

    abstract:Background:Functional sugar alcohols have been widely used in the food, medicine, and pharmaceutical industries for their unique properties. Among these, erythritol is a zero calories sweetener produced by the yeast Yarrowia lipolytica. However, in wild-type strains, erythritol is produced with low productivity and yie...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-020-01815-8

    authors: Wang N,Chi P,Zou Y,Xu Y,Xu S,Bilal M,Fickers P,Cheng H

    更新日期:2020-10-20 00:00:00

  • Scale-up and evaluation of high solid ionic liquid pretreatment and enzymatic hydrolysis of switchgrass.

    abstract:BACKGROUND:Ionic liquid (IL) pretreatment is receiving significant attention as a potential process that enables fractionation of lignocellulosic biomass and produces high yields of fermentable sugars suitable for the production of renewable fuels. However, successful optimization and scale up of IL pretreatment involv...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-154

    authors: Li C,Tanjore D,He W,Wong J,Gardner JL,Sale KL,Simmons BA,Singh S

    更新日期:2013-10-25 00:00:00

  • In situ label-free imaging of hemicellulose in plant cell walls using stimulated Raman scattering microscopy.

    abstract:BACKGROUND:Plant hemicellulose (largely xylan) is an excellent feedstock for renewable energy production and second only to cellulose in abundance. Beyond a source of fermentable sugars, xylan constitutes a critical polymer in the plant cell wall, where its precise role in wall assembly, maturation, and deconstruction ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0669-9

    authors: Zeng Y,Yarbrough JM,Mittal A,Tucker MP,Vinzant TB,Decker SR,Himmel ME

    更新日期:2016-11-22 00:00:00

  • Metabolic engineering of Clostridium beijerinckii to improve glycerol metabolism and furfural tolerance.

    abstract:Background:Inefficient utilization of glycerol by Clostridium beijerinckii (Cb) is a major impediment to adopting glycerol metabolism as a strategy for increasing NAD(P)H regeneration, which would in turn, alleviate the toxicity of lignocellulose-derived microbial inhibitory compounds (LDMICs, e.g., furfural), and impr...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1388-9

    authors: Agu CV,Ujor V,Ezeji TC

    更新日期:2019-03-09 00:00:00

  • Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar.

    abstract:BACKGROUND:Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The effects of incubation ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-42

    authors: Wang K,Yang H,Wang W,Sun RC

    更新日期:2013-03-21 00:00:00

  • Oleaginous yeast platform for producing biofuels via co-solvent hydrothermal liquefaction.

    abstract:BACKGROUND:Oleaginous microorganisms are attractive feedstock for production of liquid biofuels. Direct hydrothermal liquefaction (HTL) is an efficient route that converts whole, wet biomass into an energy-dense liquid fuel precursor, called 'biocrude'. HTL represents a promising alternative to conventional lipid extra...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0345-5

    authors: Jena U,McCurdy AT,Warren A,Summers H,Ledbetter RN,Hoekman SK,Seefeldt LC,Quinn JC

    更新日期:2015-10-13 00:00:00

  • Integration of pulp and paper technology with bioethanol production.

    abstract:BACKGROUND:Despite decades of work and billions of dollars of investments in laboratory and pilot plant projects, commercial production of cellulosic ethanol is only now beginning to emerge. Because of: (1)high technical risk coupled with; (2) high capital investment cost relative to ethanol product value, investors ha...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-13

    authors: Phillips RB,Jameel H,Chang HM

    更新日期:2013-01-28 00:00:00

  • Bioelectrochemical production of hydrogen in an innovative pressure-retarded osmosis/microbial electrolysis cell system: experiments and modeling.

    abstract:BACKGROUND:While microbial electrolysis cells (MECs) can simultaneously produce bioelectrochemical hydrogen and treat wastewater, they consume considerable energy to overcome the unfavorable thermodynamics, which is not sustainable and economically feasible in practical applications. This study presents a proof-of-conc...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0305-0

    authors: Yuan H,Lu Y,Abu-Reesh IM,He Z

    更新日期:2015-08-14 00:00:00

  • Alteration of leaf shape, improved metal tolerance, and productivity of seed by overexpression of CsHMA3 in Camelina sativa.

    abstract:BACKGROUND:Camelina sativa (L.) Crantz, known by such popular names as "gold-of-pleasure" and "false flax," is an alternative oilseed crop for biofuel production and can be grown in harsh environments. Considerable interest is now being given to the new concept of the development of a fusion plant which can be used as ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-96

    authors: Park W,Feng Y,Ahn SJ

    更新日期:2014-06-22 00:00:00

  • Effects of enzyme feeding strategy on ethanol yield in fed-batch simultaneous saccharification and fermentation of spruce at high dry matter.

    abstract:BACKGROUND:To make lignocellulosic fuel ethanol economically competitive with fossil fuels, it is necessary to reduce the production cost. One way to achieve this is by increasing the substrate concentration in the production process, and thus reduce the energy demand in the final distillation of the fermentation broth...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-3-14

    authors: Hoyer K,Galbe M,Zacchi G

    更新日期:2010-06-25 00:00:00

  • The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973.

    abstract:Background:Cyanobacteria have shown promising potential for the production of various biofuels and chemical feedstocks. Synechococcus elongatus UTEX 2973 is a fast-growing strain with pronounced tolerance to high temperatures and illumination. Hence, this strain appears to be ideal for the development of photosynthetic...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1215-8

    authors: Tan X,Hou S,Song K,Georg J,Klähn S,Lu X,Hess WR

    更新日期:2018-08-04 00:00:00

  • The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles.

    abstract::Biorefineries, designed for the production of lignocellulose-based chemicals and fuels, are receiving increasing attention from the public, governments, and industries. A major obstacle for biorefineries to advance to commercial scale is the high cost of the enzymes required to derive the fermentable sugars from the f...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章,评审

    doi:10.1186/s13068-019-1571-z

    authors: Novy V,Nielsen F,Seiboth B,Nidetzky B

    更新日期:2019-10-08 00:00:00

  • Improving pentose fermentation by preventing ubiquitination of hexose transporters in Saccharomyces cerevisiae.

    abstract:BACKGROUND:Engineering of the yeast Saccharomyces cerevisiae for improved utilization of pentose sugars is vital for cost-efficient cellulosic bioethanol production. Although endogenous hexose transporters (Hxt) can be engineered into specific pentose transporters, they remain subjected to glucose-regulated protein deg...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0573-3

    authors: Nijland JG,Vos E,Shin HY,de Waal PP,Klaassen P,Driessen AJ

    更新日期:2016-07-26 00:00:00

  • Engineering a Streptomyces coelicolor biosynthesis pathway into Escherichia coli for high yield triglyceride production.

    abstract:BACKGROUND:Microbial lipid production represents a potential alternative feedstock for the biofuel and oleochemical industries. Since Escherichia coli exhibits many genetic, technical, and biotechnological advantages over native oleaginous bacteria, we aimed to construct a metabolically engineered E. coli strain capabl...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0172-0

    authors: Comba S,Sabatini M,Menendez-Bravo S,Arabolaza A,Gramajo H

    更新日期:2014-12-24 00:00:00