Abstract:
BACKGROUND:Low- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental glucose fluctuation for their growth and competitiveness. In Neurospora crassa, the dual-affinity glucose transport system consists of a low-affinity glucose transporter GLT-1 and two high-affinity glucose transporters HGT-1/HGT-2, which play diverse roles in glucose transport, carbon metabolism, and cellulase expression regulation. However, the regulation of this dual-transporter system in response to environmental glucose fluctuation is not yet clear. RESULTS:In this study, we report that a regulation module consisting of a downstream transcription factor COL-26 and an upstream non-transporting glucose sensor RCO-3 regulates the dual-affinity glucose transport system in N. crassa. COL-26 directly binds to the promoter regions of glt-1, hgt-1, and hgt-2, whereas RCO-3 is an upstream factor of the module whose deletion mutant resembles the Δcol-26 mutant phenotypically. Transcriptional profiling analysis revealed that Δcol-26 and Δrco-3 mutants had similar transcriptional profiles, and both mutants had impaired response to a glucose gradient. We also showed that the AMP-activated protein kinase (AMPK) complex is involved in regulation of the glucose transporters. AMPK is required for repression of glt-1 expression in starvation conditions by inhibiting the activity of RCO-3. CONCLUSIONS:RCO-3 and COL-26 form an external-to-internal module that regulates the glucose dual-affinity transport system. Transcription factor COL-26 was identified as the key regulator. AMPK was also involved in the regulation of the dual-transporter system. Our findings provide novel insight into the molecular basis of glucose uptake and signaling in filamentous fungi, which may aid in the rational design of fungal strains for industrial purposes.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Li J,Liu Q,Li J,Lin L,Li X,Zhang Y,Tian Cdoi
10.1186/s13068-021-01877-2subject
Has Abstractpub_date
2021-01-28 00:00:00pages
33issue
1issn
1754-6834pii
10.1186/s13068-021-01877-2journal_volume
14pub_type
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2018-08-02 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0631-x
更新日期:2016-10-14 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1292-8
更新日期:2018-10-27 00:00:00
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2015-02-08 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-7-16
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-4-3
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1601-x
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0540-z
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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