Abstract:
BACKGROUND:Furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulose biomass to monomeric sugars, but the response of acetone butanol ethanol (ABE) producing Clostridium beijerinckii NCIMB 8052 to this compound at the molecular level is unknown. To discern the effect of furfural on C. beijerinckii and to gain insight into molecular mechanisms of action and detoxification, physiological changes of furfural-stressed cultures during acetone butanol ethanol (ABE) fermentation were studied, and differentially expressed genes were profiled by genome-wide transcriptional analysis. RESULTS:A total of 5,003 C. beijerinckii NCIMB 8052 genes capturing about 99.7% of the genome were examined. About 111 genes were differentially expressed (up- or down-regulated) by C. beijerinckii when it was challenged with furfural at acidogenic growth phase compared with 721 genes that were differentially expressed (up- or down-regulated) when C. beijerinckii was challenged with furfural at solventogenic growth phase. The differentially expressed genes include genes related to redox and cofactors, membrane transporters, carbohydrate, amino sugar and nucleotide sugar metabolisms, heat shock proteins, DNA repair, and two-component signal transduction system. While C. beijerinckii exposed to furfural stress during the acidogenic growth phase produced 13% more ABE than the unstressed control, ABE production by C. beijerinckii ceased following exposure to furfural stress during the solventogenic growth phase. CONCLUSION:Genome-wide transcriptional response of C. beijerinckii to furfural stress was investigated for the first time using microarray analysis. Stresses emanating from ABE accumulation in the fermentation medium; redox balance perturbations; and repression of genes that code for the phosphotransferase system, cell motility and flagellar proteins (and combinations thereof) may have caused the premature termination of C. beijerinckii 8052 growth and ABE production following furfural challenge at the solventogenic phase.This study provides insights into basis for metabolic engineering of C. beijerinckii NCIMB 8052 for enhanced tolerance of lignocellulose-derived microbial inhibitory compounds, thereby improving bioconversion of lignocellulose biomass hydrolysates to biofuels and chemicals. Indeed, two enzymes encoded by Cbei_3974 and Cbei_3904 belonging to aldo/keto reductase (AKR) and short-chain dehydrogenase/reductase (SDR) families have been identified to be involved in furfural detoxification and tolerance.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Zhang Y,Ezeji TCdoi
10.1186/1754-6834-6-66subject
Has Abstractpub_date
2013-05-04 00:00:00pages
66issue
1issn
1754-6834pii
1754-6834-6-66journal_volume
6pub_type
杂志文章abstract:Background:Plant biomass is an abundant but underused feedstock for bioenergy production due to its complex and variable composition, which resists breakdown into fermentable sugars. These feedstocks, however, are routinely degraded by many uncommercialized microbes such as anaerobic gut fungi. These gut fungi express ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1292-8
更新日期:2018-10-27 00:00:00
abstract::Biorefineries, designed for the production of lignocellulose-based chemicals and fuels, are receiving increasing attention from the public, governments, and industries. A major obstacle for biorefineries to advance to commercial scale is the high cost of the enzymes required to derive the fermentable sugars from the f...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章,评审
doi:10.1186/s13068-019-1571-z
更新日期:2019-10-08 00:00:00
abstract:BACKGROUND:Switchgrass (Panicum virgatum L.) is a warm-season C4 grass that is a target lignocellulosic biofuel species. In many regions, drought stress is one of the major limiting factors for switchgrass growth. The objective of this study was to evaluate the drought tolerance of 49 switchgrass genotypes. The relativ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0342-8
更新日期:2015-09-22 00:00:00
abstract:BACKGROUND:Engineering of the yeast Saccharomyces cerevisiae for improved utilization of pentose sugars is vital for cost-efficient cellulosic bioethanol production. Although endogenous hexose transporters (Hxt) can be engineered into specific pentose transporters, they remain subjected to glucose-regulated protein deg...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0573-3
更新日期:2016-07-26 00:00:00
abstract:BACKGROUND:Molasses is a dense and saline by-product of the sugar agroindustry. Its high organic content potentially fuels a myriad of renewable products of industrial interest. However, the biotechnological exploitation of molasses is mainly hampered by the high concentration of salts, an issue that is nowadays tackle...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0701-8
更新日期:2017-01-31 00:00:00
abstract:BACKGROUND:To ensure reliable sources of energy and raw materials, the utilization of sustainable biomass has considerable advantages over petroleum-based energy sources. Photosynthetic algae have attracted attention as a third-generation feedstock for biofuel production, because algae cultivation does not directly com...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-014-0183-x
更新日期:2014-12-31 00:00:00
abstract:Background:Irpex lacteus is one of the most potent white rot fungi for biological pretreatment of lignocellulose for second biofuel production. To elucidate the underlying molecular mechanism involved in lignocellulose deconstruction, genomic and transcriptomic analyses were carried out for I. lacteus CD2 grown in subm...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1060-9
更新日期:2018-03-02 00:00:00
abstract:UNLABELLED: BACKGROUND:The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two conversion systems (simultaneous saccharification and fermentation using yeast and fungal ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-49
更新日期:2012-07-19 00:00:00
abstract:BACKGROUND:In this study, the dilute maleic acid pretreatment of wheat straw is optimized, using pretreatment time, temperature and maleic acid concentration as design variables. A central composite design was applied to the experimental set up. The response factors used in this study are: (1) glucose benefits from imp...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-2-31
更新日期:2009-12-21 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-4-38
更新日期:2011-10-12 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1177-x
更新日期:2018-06-25 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01688-x
更新日期:2020-03-20 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0434-0
更新日期:2016-01-26 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0375-z
更新日期:2015-11-25 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0633-8
更新日期:2016-10-18 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1480-1
更新日期:2019-06-08 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0445-x
更新日期:2016-02-04 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1461-4
更新日期:2019-05-11 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0945-3
更新日期:2017-11-09 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1641-2
更新日期:2020-01-22 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1287-5
更新日期:2018-10-16 00:00:00
abstract::Editorial introduction to the thematic series on the life and lifework of Mary Mandels. ...
journal_title:Biotechnology for biofuels
pub_type: 社论
doi:10.1186/1754-6834-2-23
更新日期:2009-09-01 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1619-0
更新日期:2019-12-05 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01743-7
更新日期:2020-06-09 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1412-0
更新日期:2019-03-22 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-79
更新日期:2012-10-26 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0581-3
更新日期:2016-08-11 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1280-z
更新日期:2018-10-11 00:00:00
abstract:BACKGROUND:Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to 'hydrolysate toxicity,' a maj...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0233-z
更新日期:2015-03-31 00:00:00
abstract:BACKGROUND:In addition to efficient pentose utilization, high inhibitor tolerance is a key trait required in any organism used for economically viable industrial bioethanol production with lignocellulose biomass. Although recent work has succeeded in establishing efficient xylose fermentation in robust industrial Sacch...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-120
更新日期:2013-08-26 00:00:00