Abstract:
Background:α-l-Arabinofuranosidase (ARA), a debranching enzyme that can remove arabinose substituents from arabinoxylan and arabinoxylooligomers (AXOS), promotes the hydrolysis of the arabinoxylan fraction of biomass; however, the impact of ARA on the overall digestibility of cellulose is controversial. In this study, we investigated the effects of the addition of ARA on cellulase hydrolytic action. Results:We found that approximately 15% of the xylan was converted into AXOS during the hydrolysis of aqueous ammonia-pretreated corn stover and that this AXOS fraction was approximately 12% substituted with arabinose. The addition of ARA removes a portion of the arabinose decoration, but the resulting less-substituted AXOS inhibited cellulase action much more effectively; showing an increase of 45.7%. Kinetic experiments revealed that AXOS with a lower degree of arabinose substitution showed stronger affinity for the active site of cellobiohydrolase, which could be the mechanism of increased inhibition. Conclusions:Our findings strongly suggest that the ratio of ARA and other xylanases should be carefully selected to avoid the strong inhibition caused by the less-substituted AXOS during the hydrolysis of arabinoxylan-containing biomass. This study advances our understanding of the inhibitory mechanism of xylooligomers and provides critical new insights into the relationship of ARA addition and cellulose digestibility.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Xin D,Chen X,Wen P,Zhang Jdoi
10.1186/s13068-019-1412-0subject
Has Abstractpub_date
2019-03-22 00:00:00pages
64issn
1754-6834pii
1412journal_volume
12pub_type
杂志文章abstract:BACKGROUND:Non-productive cellulase adsorption onto lignin has always been deemed to negatively affect the enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding enzyme-lignin interactions is essential for the development of enzyme mixtures, the processes of lignocellulose hydrolysis, and the gene...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-7-38
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pub_type: 杂志文章
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更新日期:2020-06-03 00:00:00
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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更新日期:2014-10-18 00:00:00
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pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2018-04-09 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2012-05-06 00:00:00
abstract::[This retracts the article DOI: 10.1186/s13068-017-0920-z.]. ...
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pub_type: 杂志文章,撤回出版物
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更新日期:2018-04-06 00:00:00
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pub_type: 杂志文章
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更新日期:2015-08-14 00:00:00
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pub_type: 杂志文章
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更新日期:2015-10-13 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2017-10-30 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2019-04-30 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2013-05-04 00:00:00