Abstract:
BACKGROUND:To ensure reliable sources of energy and raw materials, the utilization of sustainable biomass has considerable advantages over petroleum-based energy sources. Photosynthetic algae have attracted attention as a third-generation feedstock for biofuel production, because algae cultivation does not directly compete with agricultural resources, including the requirement for productive land and fresh water. In particular, cyanobacteria are a promising biomass feedstock because of their high photosynthetic capability. RESULTS:In the present study, the expression of the flv3 gene, which encodes a flavodiiron protein involved in alternative electron flow (AEF) associated with NADPH-coupled O2 photoreduction in photosystem I, was enhanced in Synechocystis sp. PCC6803. Overexpression of flv3 improved cell growth with corresponding increases in O2 evolution, intracellular ATP level, and turnover of the Calvin cycle. The combination of in vivo (13)C-labeling of metabolites and metabolomic analysis confirmed that the photosynthetic carbon flow was enhanced in the flv3-overexpressing strain. CONCLUSIONS:Overexpression of flv3 improved cell growth and glycogen production in the recombinant Synechocystis sp. PCC6803. Direct measurement of metabolic turnover provided conclusive evidence that CO2 incorporation is enhanced by the flv3 overexpression. Increase in O2 evolution and ATP accumulation indicates enhancement of the AEF. Overexpression of flv3 improves photosynthesis in the Synechocystis sp. PCC6803 by enhancement of the AEF.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Hasunuma T,Matsuda M,Senga Y,Aikawa S,Toyoshima M,Shimakawa G,Miyake C,Kondo Adoi
10.1186/s13068-014-0183-xsubject
Has Abstractpub_date
2014-12-31 00:00:00pages
493issue
1issn
1754-6834pii
183journal_volume
7pub_type
杂志文章abstract:BACKGROUND:The filamentous fungus Trichoderma reesei is widely utilized in industry for cellulase production, but its xylanase activity must be improved to enhance the accessibility of lignocellulose to cellulases. Several transcription factors play important roles in this progress; however, nearly all the reported tra...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0878-x
更新日期:2017-08-03 00:00:00
abstract:Background:Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality butyric acid used in food and medicine, is more dependent on microbiological production methods. Hence, the bio-o...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01741-9
更新日期:2020-06-03 00:00:00
abstract:BACKGROUND:Large-scale production of effective cellulose hydrolytic enzymes is the key to the bioconversion of agricultural residues to ethanol. The goal of this study was to develop a rice plant as a bioreactor for the large-scale production of cellulose hydrolytic enzymes via genetic transformation, and to simultaneo...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-4-58
更新日期:2011-12-10 00:00:00
abstract:BACKGROUND:There are many different types of pretreatment carried out to prepare cellulosic substrates for fermentation. In this study, one- and two-stage hydrothermal pretreatment were carried out to determine their effects on subsequent fermentations. The two substrates were found to behave differently during ferment...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0446-9
更新日期:2016-02-03 00:00:00
abstract::Microalgae hold great promises as sustainable cellular factories for the production of alternative fuels, feeds, and biopharmaceuticals for human health. While the biorefinery approach for fuels along with the coproduction of high-value compounds with industrial, therapeutic, or nutraceutical applications have the pot...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章,评审
doi:10.1186/s13068-019-1635-0
更新日期:2019-12-23 00:00:00
abstract:Background:Microalgae are widely be used in carbon sequestration, food supplements, natural pigments, polyunsaturated fatty acids, biofuel applications, and wastewater treatment. However, the difficulties incurred in algae cell separation and harvesting, and the exorbitant cost required to overcome these challenges, ar...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01734-8
更新日期:2020-05-29 00:00:00
abstract:BACKGROUND:n-Butanol can serve as an excellent gasoline substitute. Naturally, it is produced by some Clostridia species which, however, exhibit only limited suitability for industrial n-butanol production. The yeast Saccharomyces cerevisiae would be an ideal host due to its high robustness in fermentation processes. N...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0673-0
更新日期:2016-11-25 00:00:00
abstract:Background:Saccharification is the most crucial and cost-intensive process in second generation biofuel production. The deficiency of β-glucosidase in commercial enzyme leads to incomplete biomass hydrolysis. The decomposition of biomass at high temperature environments leads us to isolate thermotolerant microbes with ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0932-8
更新日期:2017-10-30 00:00:00
abstract:BACKGROUND:In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofuel from biomass is the enzymatic saccharification. Many of the commercial cellulase enzyme cocktails...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0587-x
更新日期:2016-08-22 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-173
更新日期:2013-11-29 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01788-8
更新日期:2020-08-26 00:00:00
abstract:BACKGROUND:Ionic liquid (IL) pretreatment is receiving significant attention as a potential process that enables fractionation of lignocellulosic biomass and produces high yields of fermentable sugars suitable for the production of renewable fuels. However, successful optimization and scale up of IL pretreatment involv...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-154
更新日期:2013-10-25 00:00:00
abstract:BACKGROUND:Coordination of synthesis and assembly of the polymeric components of cell walls is essential for plant growth and development. Given the degree of co-mingling and cross-linking among cell wall components, cellulose organization must be dependent on the organization of other polymers such as lignin. Here we ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0540-z
更新日期:2016-06-17 00:00:00
abstract:BACKGROUND:Simultaneous saccharification and fermentation (SSF) is a promising process for bioconversion of lignocellulosic biomass. High glucan loading for hydrolysis and fermentation is an efficient approach to reduce the capital costs for bio-based products production. The SSF of steam-exploded corn stover (SECS) fo...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-014-0167-x
更新日期:2014-12-04 00:00:00
abstract:BACKGROUND:D-2,3-butanediol has many industrial applications such as chiral reagents, solvents, anti-freeze agents, and low freezing point fuels. Traditional D-2,3-butanediol producing microorganisms, such as Klebsiella pneumonia and K. xoytoca, are pathogenic and not capable of producing D-2,3-butanediol at high optic...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-7-16
更新日期:2014-01-29 00:00:00
abstract:BACKGROUND:Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic biomass. The commercial enzyme mixtures produced at industrial scales are not well characterized, and ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-1-18
更新日期:2008-12-23 00:00:00
abstract:BACKGROUND:Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. RESULTS:In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculu...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0286-z
更新日期:2015-07-25 00:00:00
abstract:Background:The need for liquid fuels in the transportation sector is increasing, and it is essential to develop industrially sustainable processes that simultaneously address the tri-fold sustainability metrics of technological feasibility, economic viability, and environmental impacts. Biorefineries based on lignocell...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0959-x
更新日期:2017-11-14 00:00:00
abstract:BACKGROUND:Non-productive cellulase adsorption onto lignin has always been deemed to negatively affect the enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding enzyme-lignin interactions is essential for the development of enzyme mixtures, the processes of lignocellulose hydrolysis, and the gene...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-7-38
更新日期:2014-03-14 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-3-21
更新日期:2010-09-15 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1140-x
更新日期:2018-05-12 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-3-1
更新日期:2010-01-04 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0370-4
更新日期:2015-11-25 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0460-y
更新日期:2016-02-20 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1066-3
更新日期:2018-03-14 00:00:00
abstract:UNLABELLED: BACKGROUND:Industrial production of biofuels and other products by cellulolytic microorganisms is of interest but hindered by the nascent state of genetic tools. Although a genetic system for Clostridium thermocellum DSM1313 has recently been developed, available methods achieve relatively low efficiency a...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-30
更新日期:2012-05-06 00:00:00
abstract:BACKGROUND:Alkaline hydrogen peroxide pretreatment catalyzed by Cu(II) 2,2'-bipyridine complexes has previously been determined to substantially improve the enzymatic hydrolysis of woody plants including hybrid poplar as a consequence of moderate delignification. In the present work, cell wall morphological and lignin ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0300-5
更新日期:2015-08-20 00:00:00
abstract:UNLABELLED: BACKGROUND:Acetoin and 2,3-butanediol are two important biorefinery platform chemicals. They are currently fermented below 40°C using mesophilic strains, but the processes often suffer from bacterial contamination. RESULTS:This work reports the isolation and identification of a novel aerobic Geobacillus s...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-88
更新日期:2012-12-06 00:00:00
abstract:Background:Xylan is a major hemicellulosic component in the cell walls of higher plants especially in the secondary walls of vascular cells which are playing important roles in physiological processes and overall mechanical strength. Being the second most abundant cell wall polymer after cellulose, xylan is an abundant...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0935-5
更新日期:2017-11-30 00:00:00
abstract:Background:l-Histidine biosynthesis is embedded in an intertwined metabolic network which renders microbial overproduction of this amino acid challenging. This is reflected in the few available examples of histidine producers in literature. Since knowledge about the metabolic interplay is limited, we systematically per...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1410-2
更新日期:2019-03-25 00:00:00