Abstract:
BACKGROUND:Enzymatic degradation of plant biomass requires a complex mixture of many different enzymes. Like most fungi, thermophilic Myceliophthora species therefore have a large set of enzymes targeting different linkages in plant polysaccharides. The majority of these enzymes have not been functionally characterized, and their role in plant biomass degradation is unknown. The biotechnological challenge is to select the right set of enzymes to efficiently degrade a particular biomass. This study describes a strategy using sexual crossing and screening with the thermophilic fungus Myceliophthora heterothallica to identify specific enzymes associated with improved sugar beet pulp saccharification. RESULTS:Two genetically diverse M. heterothallica strains CBS 203.75 and CBS 663.74 were used to generate progenies with improved growth on sugar beet pulp. One progeny, named SBP.F1.2.11, had a different genetic pattern from the parental strains and had improved saccharification activity after the growth on 3 % sugar beet pulp. The improved SBP saccharification was not explained by altered activities of the major (hemi-)cellulases. Exo-proteome analysis of progeny and parental strains after 7-day growth on sugar beet pulp showed that only 17 of the 133 secreted CAZy enzymes were more abundant in progeny SBP.F1.2.11. Particularly one enzyme belonging to the carbohydrate esterase family 5 (CE5) was more abundant in SBP.F1.2.11. This CE5-CBM1 enzyme, named as Axe1, was phylogenetically related to acetyl xylan esterases. Biochemical characterization of Axe1 confirmed de-acetylation activity with optimal activities at 75-85 °C and pH 5.5-6.0. Supplementing Axe1 to CBS 203.75 enzyme set improved release of xylose and glucose from sugar beet pulp. CONCLUSIONS:This study identified beneficial enzymes for sugar beet pulp saccharification by selecting progeny with improved growth on this particular substrate. Saccharification of sugar beet pulp was improved by supplementing enzyme mixtures with a previously uncharacterized CE5-CBM1 acetyl xylan esterase. This shows that sexual crossing and selection of M. heterothallica are the successful strategy to improve the composition of enzyme mixtures for efficient plant biomass degradation.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Aguilar-Pontes MV,Zhou M,van der Horst S,Theelen B,de Vries RP,van den Brink Jdoi
10.1186/s13068-016-0460-ysubject
Has Abstractpub_date
2016-02-20 00:00:00pages
41issn
1754-6834pii
460journal_volume
9pub_type
杂志文章abstract:BACKGROUND:Although lignin peroxidase is claimed as a key enzyme in enzyme-catalyzed lignin degradation, in vitro enzymatic degradation of lignin was not easily observed in lab-scale experiments. It implies that other factors may hinder the enzymatic degradation of lignin. Irreversible interaction between phenolic comp...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0664-1
更新日期:2016-11-15 00:00:00
abstract:Background:Xylan is a major hemicellulosic component in the cell walls of higher plants especially in the secondary walls of vascular cells which are playing important roles in physiological processes and overall mechanical strength. Being the second most abundant cell wall polymer after cellulose, xylan is an abundant...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0935-5
更新日期:2017-11-30 00:00:00
abstract:BACKGROUND:Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of lingocellulosic materials is essential for the success of these processes. RESULTS:Conversion of a...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-6
更新日期:2012-02-13 00:00:00
abstract:BACKGROUND:Oxygenic photosynthetic microorganisms such as cyanobacteria and microalgae have attracted attention as an alternative carbon source for the next generation of biofuels. Glycogen abundantly accumulated in cyanobacteria is a promising feedstock which can be converted to ethanol through saccharification and fe...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-7-88
更新日期:2014-06-11 00:00:00
abstract:Background:Astaxanthin is a natural carotenoid pigment with tremendous antioxidant activity and great commercial value. Microbial production of astaxanthin via metabolic engineering has become a promising alternative. Although great efforts have been conducted by tuning the heterologous modules and precursor pools, the...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1227-4
更新日期:2018-08-23 00:00:00
abstract:Background:Populus (poplar) tree species including hybrid varieties are considered as promising biomass feedstock for biofuels and biochemicals production due to their fast growing, short vegetative cycle, and widely distribution. In this work, poplar was pretreated with acetic acid (AC) to produce xylooligosaccharides...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1423-x
更新日期:2019-04-15 00:00:00
abstract:BACKGROUND:Rut-C30 is a cellulase-hyperproducing Trichoderma reesei strain and, consequently, became the ancestor of most industry strains used in the production of plant cell wall-degrading enzymes, in particular cellulases. Due to three rounds of undirected mutagenesis its genetic background differs from the wild-typ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-014-0129-3
更新日期:2014-09-11 00:00:00
abstract:Background:The recalcitrance of lignocellulosic biomass offers a series of challenges for biochemical processing into biofuels and bio-products. For the first time, we address these challenges with a biomimetic system via a mild yet rapid Fenton reaction and lignocellulose-degrading bacterial strain Cupriavidus basilen...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1035-x
更新日期:2018-02-08 00:00:00
abstract:BACKGROUND:Mass transfer plays an important role in determining the efficiency of the biofuel conversion. However, adverse effect of shear stress from traditional agitation inhibits the cell growth and production of biofuels. How to enhance the mass transfer with less adverse effect is considered as one of the importan...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0409-6
更新日期:2015-12-23 00:00:00
abstract:BACKGROUND:The microbial community in anaerobic digestion is mainly monitored by means of DNA-based methods. This may lead to incorrect interpretation of the community parameters, because microbial abundance does not necessarily reflect activity. In this research, the difference between microbial community response on ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0652-5
更新日期:2016-11-09 00:00:00
abstract:BACKGROUND:Molasses is a dense and saline by-product of the sugar agroindustry. Its high organic content potentially fuels a myriad of renewable products of industrial interest. However, the biotechnological exploitation of molasses is mainly hampered by the high concentration of salts, an issue that is nowadays tackle...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0701-8
更新日期:2017-01-31 00:00:00
abstract:UNLABELLED: BACKGROUND:Industrial production of biofuels and other products by cellulolytic microorganisms is of interest but hindered by the nascent state of genetic tools. Although a genetic system for Clostridium thermocellum DSM1313 has recently been developed, available methods achieve relatively low efficiency a...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-30
更新日期:2012-05-06 00:00:00
abstract:BACKGROUND:β-Glucosidase is an important component of the cellulase enzyme system. It does not only participate in cellulose degradation, it also plays an important role in hydrolyzing cellulose to fermentable glucose by relieving the inhibition of exoglucanase and endoglucanase from cellobiose. Therefore, the glucose-...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-31
更新日期:2012-07-11 00:00:00
abstract:Background:The recombinant Saccharomyces cerevisiae strains that acquired the ability to utilize xylose through metabolic and evolutionary engineering exhibit good performance when xylose is the sole carbon source in the medium (designated the X stage in the present work). However, the xylose consumption rate of strain...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1112-1
更新日期:2018-04-16 00:00:00
abstract:Background:Lavender (Lavandula angustifolia) and lavandin (a sterile hybrid of L. angustifolia × L. latifolia) essential oils are among those most commonly used in the world for various industrial purposes, including perfumes, pharmaceuticals and cosmetics. The solid residues from aromatic plant distillation such as la...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1218-5
更新日期:2018-08-02 00:00:00
abstract:Background:The industrial vinegar residue (VR) from solid-state fermentation, mainly cereals and their bran, will be a potential feedstock for future biofuels because of their low cost and easy availability. However, utilization of VR for butanol production has not been as much optimized as other sources of lignocellul...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01751-7
更新日期:2020-06-24 00:00:00
abstract:BACKGROUND:Despite decades of work and billions of dollars of investments in laboratory and pilot plant projects, commercial production of cellulosic ethanol is only now beginning to emerge. Because of: (1)high technical risk coupled with; (2) high capital investment cost relative to ethanol product value, investors ha...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-13
更新日期:2013-01-28 00:00:00
abstract:BACKGROUND:Brown algae are promising feedstocks for biofuel production with inherent advantages of no structural lignin, high growth rate, and no competition for land and fresh water. However, it is difficult for one microorganism to convert all components of brown algae with different oxidoreduction potentials to etha...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0494-1
更新日期:2016-04-01 00:00:00
abstract:Background:To increase the production of bioethanol, a two-step process based on hydrothermal and dilute alkaline treatment was applied to reduce the natural resistance of biomass. However, the process required a large amount of water and a long operation time due to the solid/liquid separation before the alkaline trea...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1140-x
更新日期:2018-05-12 00:00:00
abstract:BACKGROUND:Solid-state anaerobic digestion (SS-AD) has been increasingly used for lignocellulosic biomass treatment. However, the separate reactor required for pretreatment prior digestion, poor treatment capacity, and process stability inhibit further development of the SS-AD. In this study, a novel method called SS-A...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0530-1
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND:The diverse microbial communities in agricultural biogas fermenters are assumed to be well adapted for the anaerobic transformation of plant biomass to methane. Compared to natural systems, biogas reactors are limited in their hydrolytic potential. The reasons for this are not understood. RESULTS:In this pa...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0534-x
更新日期:2016-06-07 00:00:00
abstract:BACKGROUND:Bioaugmentation or an addition of the desired microorganisms or specialized microbial strains into the anaerobic digesters can enhance the performance of microbial community in the hydrogen production process. Most of the studies focused on a bioaugmentation of native microorganisms capable of producing hydr...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0375-z
更新日期:2015-11-25 00:00:00
abstract:Background:Irpex lacteus is one of the most potent white rot fungi for biological pretreatment of lignocellulose for second biofuel production. To elucidate the underlying molecular mechanism involved in lignocellulose deconstruction, genomic and transcriptomic analyses were carried out for I. lacteus CD2 grown in subm...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1060-9
更新日期:2018-03-02 00:00:00
abstract:: The production of cellulosic ethanol from biomass is considered a promising alternative to reliance on diminishing supplies of fossil fuels, providing a sustainable option for fuels production in an environmentally compatible manner. The conversion of lignocellulosic biomass to biofuels through a biological route usu...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-15
更新日期:2013-01-28 00:00:00
abstract:BACKGROUND:n-Butanol can serve as an excellent gasoline substitute. Naturally, it is produced by some Clostridia species which, however, exhibit only limited suitability for industrial n-butanol production. The yeast Saccharomyces cerevisiae would be an ideal host due to its high robustness in fermentation processes. N...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0673-0
更新日期:2016-11-25 00:00:00
abstract:BACKGROUND:Considerable works have been reported concerning the obstruction of enzymatic hydrolysis efficiency by lignin. However, there is a lack of information about the influence of lignin on the adsorption of cellulases on cellulose, along with the hydrolytic activity of the cellulases adsorbed on lignin. In additi...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0434-0
更新日期:2016-01-26 00:00:00
abstract:BACKGROUND:Renewable energy production is currently a major issue worldwide. Biogas is a promising renewable energy carrier as the technology of its production combines the elimination of organic waste with the formation of a versatile energy carrier, methane. In consequence of the complexity of the microbial communiti...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-41
更新日期:2012-07-12 00:00:00
abstract:Background:For bioethanol production from lignocellulosic biomass, phenolics derived from pretreatment have been generally considered as highly inhibitory towards enzymatic hydrolysis and fermentation. As phenolics are produced from lignin degradation during pretreatment, it is likely that the pretreatment will exert a...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01686-z
更新日期:2020-03-11 00:00:00
abstract:BACKGROUND:Decomposition of biomass for biogas production can be practiced under wet and dry fermentation conditions. In contrast to the dry fermentation technology, wet fermentation is characterized by a high liquid content and a relatively low total solid content. In this study, the composition and functional potenti...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-014-0193-8
更新日期:2015-02-08 00:00:00
abstract:BACKGROUND:Photosynthetic production of chemicals and fuels by recycling CO2 in cyanobacteria is a promising solution facing energy shortage and resource declination. Ethanol is an attractive and demonstrative biofuel product, and ethanol synthesis in cyanobacteria has been achieved by assembling of a pathway consistin...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0367-z
更新日期:2015-11-21 00:00:00