Abstract：
UNLABELLED: BACKGROUND:Industrial production of biofuels and other products by cellulolytic microorganisms is of interest but hindered by the nascent state of genetic tools. Although a genetic system for Clostridium thermocellum DSM1313 has recently been developed, available methods achieve relatively low efficiency and similar plasmids can transform C. thermocellum at dramatically different efficiencies. RESULTS:We report an increase in transformation efficiency of C. thermocellum for a variety of plasmids by using DNA that has been methylated by Escherichia coli Dam but not Dcm methylases. When isolated from a dam+dcm+E. coli strain, pAMG206 transforms C. thermocellum 100-fold better than the similar plasmid pAMG205, which contains an additional Dcm methylation site in the pyrF gene. Upon removal of Dcm methylation, transformation with pAMG206 showed a four- to seven-fold increase in efficiency; however, transformation efficiency of pAMG205 increased 500-fold. Removal of the Dcm methylation site from the pAMG205 pyrF gene via silent mutation resulted in increased transformation efficiencies equivalent to that of pAMG206. Upon proper methylation, transformation efficiency of plasmids bearing the pMK3 and pB6A origins of replication increased ca. three orders of magnitude. CONCLUSIONS:E. coli Dcm methylation decreases transformation efficiency in C. thermocellum DSM1313. The use of properly methylated plasmid DNA should facilitate genetic manipulation of this industrially relevant bacterium.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Guss AM,Olson DG,Caiazza NC,Lynd LRdoi
10.1186/1754-6834-5-30subject
Has Abstractpub_date
2012-05-06 00:00:00pages
30issue
1issn
1754-6834pii
1754-6834-5-30journal_volume
5pub_type
杂志文章abstract：Background:Triacylglycerols (TAGs) rich in medium-chain fatty acids (MCFAs, C10-14 fatty acids) are valuable feedstocks for biofuels and chemicals. Natural sources of TAGs rich in MCFAs are restricted to a limited number of plant species, which are unsuitable for mass agronomic production. Instead, the modification of ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1177-x
更新日期：2018-06-25 00:00:00
abstract：BACKGROUND:Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The effects of incubation ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-42
更新日期：2013-03-21 00:00:00
abstract：Background:Microbial fuel cell (MFC) convokes microorganism to convert biomass into electricity. However, most well-known electrogenic strains cannot directly use glucose to produce valuable products. Zymomonas mobilis, a promising bacterium for ethanol production, owns special Entner-Doudoroff pathway with less ATP an...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01672-5
更新日期：2020-03-05 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0589-8
更新日期：2016-08-24 00:00:00
abstract：Background:S-Adenosylmethionine (SAM) is a critical cofactor involved in many biochemical reactions. However, the low fermentation titer of SAM in methionine-free medium hampers commercial-scale production. The SAM synthesis pathway is specially related to the tricarboxylic acid (TCA) cycle in Bacillus amyloliquefacien...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1554-0
更新日期：2019-09-09 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1060-9
更新日期：2018-03-02 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1044-9
更新日期：2018-02-15 00:00:00
abstract：BACKGROUND:A key barrier that limits the full potential of biological processes to create new, sustainable materials and fuels from plant fibre is limited enzyme accessibility to polysaccharides and lignin that characterize lignocellulose networks. Moreover, the heterogeneity of lignocellulosic substrates means that di...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-014-0176-9
更新日期：2014-12-31 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-181
更新日期：2013-12-16 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-83
更新日期：2012-11-22 00:00:00
abstract：Background:Biomass-derived jet fuel is an alternative jet fuel (AJF) showing promise of reducing the dependence on fossil fuel and greenhouse gas emissions. Hydroprocessed esters and fatty acids (HEFA) concept is also known as one of the pathways for producing bio jet fuel. HEFA fuel was approved by the American Societ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0945-3
更新日期：2017-11-09 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-31
更新日期：2012-07-11 00:00:00
abstract：:Editorial introduction to the thematic series on the life and lifework of Mary Mandels. ...
journal_title：Biotechnology for biofuels
pub_type： 社论
doi：10.1186/1754-6834-2-23
更新日期：2009-09-01 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1073-4
更新日期：2018-03-22 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0747-7
更新日期：2017-03-09 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-3-16
更新日期：2010-07-02 00:00:00
abstract：BACKGROUND:Glycoside hydrolases are important for various industrial and scientific applications. Determination of their temperature as well as pH optima and range is crucial to evaluate whether an enzyme is suitable for application in a biotechnological process. These basic characteristics of enzymes are generally det...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0923-9
更新日期：2017-10-11 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1619-0
更新日期：2019-12-05 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1641-2
更新日期：2020-01-22 00:00:00
abstract：Background:Cyanobacteria, a group of photosynthetic prokaryotes, are being increasingly explored for direct conversion of carbon dioxide to useful chemicals. However, efforts to engineer these photoautotrophs have resulted in low product titers. This may be ascribed to the bottlenecks in metabolic pathways, which need ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01727-7
更新日期：2020-05-18 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-120
更新日期：2013-08-26 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0370-4
更新日期：2015-11-25 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0367-z
更新日期：2015-11-21 00:00:00
abstract：Background:Biogenic and biogenic-thermogenic coalbed methane (CBM) are important energy reserves for unconventional natural gas. Thus, to investigate biogenic gas formation mechanisms, a series of fresh coal samples from several representative areas of China were analyzed to detect hydrogen-producing bacteria and metha...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1237-2
更新日期：2018-09-08 00:00:00
abstract：BACKGROUND:Renewable energy production is currently a major issue worldwide. Biogas is a promising renewable energy carrier as the technology of its production combines the elimination of organic waste with the formation of a versatile energy carrier, methane. In consequence of the complexity of the microbial communiti...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-41
更新日期：2012-07-12 00:00:00
abstract：BACKGROUND:Engineering of the yeast Saccharomyces cerevisiae for improved utilization of pentose sugars is vital for cost-efficient cellulosic bioethanol production. Although endogenous hexose transporters (Hxt) can be engineered into specific pentose transporters, they remain subjected to glucose-regulated protein deg...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0573-3
更新日期：2016-07-26 00:00:00
abstract：Background:Acid pretreatment is a common strategy used to break down the hemicellulose component of the lignocellulosic biomass to release pentoses, and a subsequent enzymatic hydrolysis step is usually applied to release hexoses from the cellulose. The hydrolysate after pretreatment and enzymatic hydrolysis containing...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01781-1
更新日期：2020-08-13 00:00:00
abstract：BACKGROUND:Cellulose amorphogenesis, described as the non-hydrolytic "opening up" or disruption of a cellulosic substrate, is becoming increasingly recognized as one of the key steps in the enzymatic deconstruction of cellulosic biomass when used as a feedstock for fuels and chemicals production. Although this process ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-51
更新日期：2012-07-24 00:00:00
abstract：BACKGROUND:The design of photobioreactor (PBR) for outdoor mass cultivation of microalgae determines the distribution of solar irradiance among cells in the culture, mode of agitation, mass transfer efficacy, and energy consumption, thus determines the productivity of the system and the cost of production. In this stud...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0633-8
更新日期：2016-10-18 00:00:00
abstract：Background:Xylan is a major hemicellulosic component in the cell walls of higher plants especially in the secondary walls of vascular cells which are playing important roles in physiological processes and overall mechanical strength. Being the second most abundant cell wall polymer after cellulose, xylan is an abundant...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0935-5
更新日期：2017-11-30 00:00:00
