Abstract:
Background:Lignin is a heterogeneous polymer representing a renewable source of aromatic and phenolic bio-derived products for the chemical industry. However, the inherent structural complexity and recalcitrance of lignin makes its conversion into valuable chemicals a challenge. Natural microbial communities produce biocatalysts derived from a large number of microorganisms, including those considered unculturable, which operate synergistically to perform a variety of bioconversion processes. Thus, metagenomic approaches are a powerful tool to reveal novel optimized metabolic pathways for lignin conversion and valorization. Results:The lignin-degrading consortium (LigMet) was obtained from a sugarcane plantation soil sample. The LigMet taxonomical analyses (based on 16S rRNA) indicated prevalence of Proteobacteria, Actinobacteria and Firmicutes members, including the Alcaligenaceae and Micrococcaceae families, which were enriched in the LigMet compared to sugarcane soil. Analysis of global DNA sequencing revealed around 240,000 gene models, and 65 draft bacterial genomes were predicted. Along with depicting several peroxidases, dye-decolorizing peroxidases, laccases, carbohydrate esterases, and lignocellulosic auxiliary (redox) activities, the major pathways related to aromatic degradation were identified, including benzoate (or methylbenzoate) degradation to catechol (or methylcatechol), catechol ortho-cleavage, catechol meta-cleavage, and phthalate degradation. A novel Paenarthrobacter strain harboring eight gene clusters related to aromatic degradation was isolated from LigMet and was able to grow on lignin as major carbon source. Furthermore, a recombinant pathway for vanillin production was designed based on novel gene sequences coding for a feruloyl-CoA synthetase and an enoyl-CoA hydratase/aldolase retrieved from the metagenomic data set. Conclusion:The enrichment protocol described in the present study was successful for a microbial consortium establishment towards the lignin and aromatic metabolism, providing pathways and enzyme sets for synthetic biology engineering approaches. This work represents a pioneering study on lignin conversion and valorization strategies based on metagenomics, revealing several novel lignin conversion enzymes, aromatic-degrading bacterial genomes, and a novel bacterial strain of potential biotechnological interest. The validation of a biosynthetic route for vanillin synthesis confirmed the applicability of the targeted metagenome discovery approach for lignin valorization strategies.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Moraes EC,Alvarez TM,Persinoti GF,Tomazetto G,Brenelli LB,Paixão DAA,Ematsu GC,Aricetti JA,Caldana C,Dixon N,Bugg TDH,Squina FMdoi
10.1186/s13068-018-1073-4subject
Has Abstractpub_date
2018-03-22 00:00:00pages
75issn
1754-6834pii
1073journal_volume
11pub_type
杂志文章abstract:Background:Microalgae are efficient producers of lipid-rich biomass, making them a key component in developing a sustainable energy source, and an alternative to fossil fuels. Chlorella species are of special interest because of their fast growth rate in photobioreactors. However, biological constraints still cast a si...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1566-9
更新日期:2019-09-16 00:00:00
abstract:BACKGROUND:Alkaline hydrogen peroxide pretreatment catalyzed by Cu(II) 2,2'-bipyridine complexes has previously been determined to substantially improve the enzymatic hydrolysis of woody plants including hybrid poplar as a consequence of moderate delignification. In the present work, cell wall morphological and lignin ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0300-5
更新日期:2015-08-20 00:00:00
abstract:BACKGROUND:In this study, the dilute maleic acid pretreatment of wheat straw is optimized, using pretreatment time, temperature and maleic acid concentration as design variables. A central composite design was applied to the experimental set up. The response factors used in this study are: (1) glucose benefits from imp...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-2-31
更新日期:2009-12-21 00:00:00
abstract:BACKGROUND:Lignocellulosic biomass is an abundant and inexpensive resource for biofuel production. Alongside its biotechnological conversion, pretreatment is essential to enable efficient enzymatic hydrolysis by making cellulose susceptible to cellulases. Wet oxidation of biomass, such as acetone/water oxidation, that ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0737-9
更新日期:2017-02-28 00:00:00
abstract:BACKGROUND:A substantial barrier to commercialization of lignocellulosic ethanol production is a lack of process specific sensors and associated control strategies that are essential for economic viability. Current sensors and analytical techniques require lengthy offline analysis or are easily fouled in situ. Raman sp...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-28
更新日期:2013-02-20 00:00:00
abstract:BACKGROUND:Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasingly important as a sustainable replacement for traditional petroleum-based production processes depe...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-021-01878-1
更新日期:2021-01-19 00:00:00
abstract:Background:Understanding the DNA methylome and its relationship with non-coding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), is essential for elucidating the molecular mechanisms underlying key biological processes in plants. Few studies have examined the functional roles of the DNA methylome ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1202-0
更新日期:2018-07-24 00:00:00
abstract:BACKGROUND:The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (GH10) from the thermophilic ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-7-115
更新日期:2014-07-29 00:00:00
abstract:BACKGROUND:In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofuel from biomass is the enzymatic saccharification. Many of the commercial cellulase enzyme cocktails...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0587-x
更新日期:2016-08-22 00:00:00
abstract:BACKGROUND:Lipid/carbohydrate content and ratio are extremely important when engineering algal cells for liquid biofuel production. However, conventional methods for such determination and quantification are not only destructive and tedious, but also energy consuming and environment unfriendly. In this study, we first ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0691-y
更新日期:2017-01-03 00:00:00
abstract:BACKGROUND:β-Glucosidase is an important component of the cellulase enzyme system. It does not only participate in cellulose degradation, it also plays an important role in hydrolyzing cellulose to fermentable glucose by relieving the inhibition of exoglucanase and endoglucanase from cellobiose. Therefore, the glucose-...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-31
更新日期:2012-07-11 00:00:00
abstract:UNLABELLED: BACKGROUND:Acetoin is an important bio-based platform chemical. However, it is usually existed as a minor byproduct of 2,3-butanediol fermentation in bacteria. RESULTS:The present study reports introducing an exogenous NAD+ regeneration sysytem into a 2,3-butanediol producing strain Klebsiella pneumoniae ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-7
更新日期:2013-01-25 00:00:00
abstract:Background:Microbial fuel cell (MFC) convokes microorganism to convert biomass into electricity. However, most well-known electrogenic strains cannot directly use glucose to produce valuable products. Zymomonas mobilis, a promising bacterium for ethanol production, owns special Entner-Doudoroff pathway with less ATP an...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01672-5
更新日期:2020-03-05 00:00:00
abstract:Background:Cyanobacteria are potential sources for third generation biofuels. Their capacity for biofuel production has been widely improved using metabolically engineered strains. In this study, we employed metabolic engineering design with target genes involved in selected processes including the fatty acid synthesis...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1349-8
更新日期:2019-01-04 00:00:00
abstract:Background:2,3-Butanediol (2,3-BDO) is a valuable chemical for industrial applications. Bacteria can produce 2,3-BDO with a high productivity, though most of their classification as pathogens makes them undesirable for the industrial-scale production. Though Saccharomyces cerevisiae (GRAS microorganism) was engineered ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1545-1
更新日期:2019-08-29 00:00:00
abstract:BACKGROUND:Alcohol-to-jet (ATJ) is one of the technical feasible biofuel technologies. It produces jet fuel from sugary, starchy, and lignocellulosic biomass, such as sugarcane, corn grain, and switchgrass, via fermentation of sugars to ethanol or other alcohols. This study assesses the ATJ biofuel production pathway f...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0702-7
更新日期:2017-01-19 00:00:00
abstract:Background:Understanding the extracellular electron transport pathways in cyanobacteria is a major factor towards developing biophotovoltaics. Stressing cyanobacteria cells environmentally and then probing changes in physiology or metabolism following a significant change in electron transfer rates is a common approach...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01788-8
更新日期:2020-08-26 00:00:00
abstract:Background:Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by converting it to xylulose. For this, the type II xylose isomerase ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1643-0
更新日期:2020-01-11 00:00:00
abstract:BACKGROUND:There are many different types of pretreatment carried out to prepare cellulosic substrates for fermentation. In this study, one- and two-stage hydrothermal pretreatment were carried out to determine their effects on subsequent fermentations. The two substrates were found to behave differently during ferment...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0446-9
更新日期:2016-02-03 00:00:00
abstract:BACKGROUND:The production of biofuels and biochemicals from grass-type plant biomass requires a complete utilisation of the plant cellulose and hemicellulosic xylan via enzymatic degradation to their constituent monosaccharides. Generally, physical and/or thermochemical pretreatments are performed to enable access for ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0519-9
更新日期:2016-05-18 00:00:00
abstract:BACKGROUND:Cyanobacteria are an attractive candidate for the production of biofuel because of their ability to capture carbon dioxide by photosynthesis and grow on non-arable land. However, because huge quantities of water are required for cultivation, strict water management is one of the greatest issues in algae- and...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-7-56
更新日期:2014-04-09 00:00:00
abstract:BACKGROUND:Hydrolysates of plant biomass used for the production of lignocellulosic biofuels typically contain sugar mixtures consisting mainly of D-glucose and D-xylose, and minor amounts of L-arabinose. The yeast Saccharomyces cerevisiae is the preferred microorganism for the fermentative production of ethanol but is...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-4-38
更新日期:2011-10-12 00:00:00
abstract:Background:Plant seed oil is an important bioresource for human food and animal feed, as well as industrial bioproducts. Therefore, increasing oil content in seeds has been one of the primary targets in the breeding programs of oilseed crops. Thraustochytrium is a marine protist that can produce a high level of very lo...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1514-8
更新日期:2019-06-29 00:00:00
abstract:BACKGROUND:Multidimensional solid-state nuclear magnetic resonance (ssNMR) spectroscopy has emerged as an indispensable technique for resolving polymer structure and intermolecular packing in primary and secondary plant cell walls. Isotope (13C) enrichment provides feasible sensitivity for measuring 2D/3D correlation s...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01858-x
更新日期:2021-01-07 00:00:00
abstract:: The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its value on market was reduced and it is becoming a "waste-stream" instead of a valuable "coproduct". The developmen...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-48
更新日期:2012-07-18 00:00:00
abstract:Background:Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS). The biofilm of Clostridium acetobutylicum confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals. However, little ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1316-4
更新日期:2018-11-20 00:00:00
abstract:BACKGROUND:Oligocelluloses and oligoxyloses are partially hydrolyzed products from lignocellulosic biomass hydrolysis. Biomass hydrolysates usually contain monosaccharides as well as various amounts of oligosaccharides. To utilize biomass hydrolysates more efficiently, it is important to identify microorganisms capable...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-7-13
更新日期:2014-01-28 00:00:00
abstract:Background:Lavender (Lavandula angustifolia) and lavandin (a sterile hybrid of L. angustifolia × L. latifolia) essential oils are among those most commonly used in the world for various industrial purposes, including perfumes, pharmaceuticals and cosmetics. The solid residues from aromatic plant distillation such as la...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-018-1218-5
更新日期:2018-08-02 00:00:00
abstract:Background:Populus (poplar) tree species including hybrid varieties are considered as promising biomass feedstock for biofuels and biochemicals production due to their fast growing, short vegetative cycle, and widely distribution. In this work, poplar was pretreated with acetic acid (AC) to produce xylooligosaccharides...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1423-x
更新日期:2019-04-15 00:00:00
abstract:BACKGROUND:Succinic acid has great potential to be a new bio-based building block for deriving a number of value-added chemicals in industry. Bio-based succinic acid production from renewable biomass can provide a feasible approach to partially alleviate the dependence of global manufacturing on petroleum refinery. To ...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01850-5
更新日期:2020-12-14 00:00:00