Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR-Cas9 system for succinic acid production from renewable biomass.

Abstract:

BACKGROUND:Succinic acid has great potential to be a new bio-based building block for deriving a number of value-added chemicals in industry. Bio-based succinic acid production from renewable biomass can provide a feasible approach to partially alleviate the dependence of global manufacturing on petroleum refinery. To improve the economics of biological processes, we attempted to explore possible solutions with a fungal cell platform. In this study, Aspergillus niger, a well-known industrial production organism for bio-based organic acids, was exploited for its potential for succinic acid production. RESULTS:With a ribonucleoprotein (RNP)-based CRISPR-Cas9 system, consecutive genetic manipulations were realized in engineering of the citric acid-producing strain A. niger ATCC 1015. Two genes involved in production of two byproducts, gluconic acid and oxalic acid, were disrupted. In addition, an efficient C4-dicarboxylate transporter and a soluble NADH-dependent fumarate reductase were overexpressed. The resulting strain SAP-3 produced 17 g/L succinic acid while there was no succinic acid detected at a measurable level in the wild-type strain using a synthetic substrate. Furthermore, two cultivation parameters, temperature and pH, were investigated for their effects on succinic acid production. The highest amount of succinic acid was obtained at 35 °C after 3 days, and low culture pH had inhibitory effects on succinic acid production. Two types of renewable biomass were explored as substrates for succinic acid production. After 6 days, the SAP-3 strain was capable of producing 23 g/L and 9 g/L succinic acid from sugar beet molasses and wheat straw hydrolysate, respectively. CONCLUSIONS:In this study, we have successfully applied the RNP-based CRISPR-Cas9 system in genetic engineering of A. niger and significantly improved the succinic acid production in the engineered strain. The studies on cultivation parameters revealed the impacts of pH and temperature on succinic acid production and the future challenges in strain development. The feasibility of using renewable biomass for succinic acid production by A. niger has been demonstrated with molasses and wheat straw hydrolysate.

journal_name

Biotechnol Biofuels

authors

Yang L,Henriksen MM,Hansen RS,Lübeck M,Vang J,Andersen JE,Bille S,Lübeck PS

doi

10.1186/s13068-020-01850-5

subject

Has Abstract

pub_date

2020-12-14 00:00:00

pages

206

issue

1

issn

1754-6834

pii

10.1186/s13068-020-01850-5

journal_volume

13

pub_type

杂志文章
  • Increased isobutanol production in Saccharomyces cerevisiae by overexpression of genes in valine metabolism.

    abstract:BACKGROUND:Isobutanol can be a better biofuel than ethanol due to its higher energy density and lower hygroscopicity. Furthermore, the branched-chain structure of isobutanol gives a higher octane number than the isomeric n-butanol. Saccharomyces cerevisiae was chosen as the production host because of its relative toler...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-4-21

    authors: Chen X,Nielsen KF,Borodina I,Kielland-Brandt MC,Karhumaa K

    更新日期:2011-07-28 00:00:00

  • Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production.

    abstract:BACKGROUND:There has been a great deal of interest in fuel productions from lignocellulosic biomass to minimize the conflict between food and fuel use. The bioconversion of xylose, which is the second most abundant sugar present after glucose in lignocellulosic biomass, is important for the development of cost effectiv...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-134

    authors: Kurosawa K,Wewetzer SJ,Sinskey AJ

    更新日期:2013-09-16 00:00:00

  • Ethanol production from mixtures of wheat straw and wheat meal.

    abstract:BACKGROUND:Bioethanol can be produced from sugar-rich, starch-rich (first generation; 1G) or lignocellulosic (second generation; 2G) raw materials. Integration of 2G ethanol with 1G could facilitate the introduction of the 2G technology. The capital cost per ton of fuel produced would be diminished and better utilizati...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-3-16

    authors: Erdei B,Barta Z,Sipos B,Réczey K,Galbe M,Zacchi G

    更新日期:2010-07-02 00:00:00

  • Correction to: Pelagibaca bermudensis promotes biofuel competence of Tetraselmis striata in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity.

    abstract::[This corrects the article DOI: 10.1186/s13068-018-1097-9.]. ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章,已发布勘误

    doi:10.1186/s13068-018-1185-x

    authors: Patidar SK,Kim SH,Kim JH,Park J,Park BS,Han MS

    更新日期:2018-07-03 00:00:00

  • Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution.

    abstract:Background:Methane, a main component of natural gas and biogas, has gained much attention as an abundant and low-cost carbon source. Methanotrophs, which can use methane as a sole carbon and energy source, are promising hosts to produce value-added chemicals from methane, but their metabolic engineering is still challe...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1574-9

    authors: Lee JK,Kim S,Kim W,Kim S,Cha S,Moon H,Hur DH,Kim SY,Na JG,Lee JW,Lee EY,Hahn JS

    更新日期:2019-09-30 00:00:00

  • Unraveling the microbiome of a thermophilic biogas plant by metagenome and metatranscriptome analysis complemented by characterization of bacterial and archaeal isolates.

    abstract:BACKGROUND:One of the most promising technologies to sustainably produce energy and to mitigate greenhouse gas emissions from combustion of fossil energy carriers is the anaerobic digestion and biomethanation of organic raw material and waste towards biogas by highly diverse microbial consortia. In this context, the mi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0581-3

    authors: Maus I,Koeck DE,Cibis KG,Hahnke S,Kim YS,Langer T,Kreubel J,Erhard M,Bremges A,Off S,Stolze Y,Jaenicke S,Goesmann A,Sczyrba A,Scherer P,König H,Schwarz WH,Zverlov VV,Liebl W,Pühler A,Schlüter A,Klocke M

    更新日期:2016-08-11 00:00:00

  • Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering.

    abstract:Background:Astaxanthin is a natural carotenoid pigment with tremendous antioxidant activity and great commercial value. Microbial production of astaxanthin via metabolic engineering has become a promising alternative. Although great efforts have been conducted by tuning the heterologous modules and precursor pools, the...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1227-4

    authors: Jin J,Wang Y,Yao M,Gu X,Li B,Liu H,Ding M,Xiao W,Yuan Y

    更新日期:2018-08-23 00:00:00

  • Pulsed addition of HMF and furfural to batch-grown xylose-utilizing Saccharomyces cerevisiae results in different physiological responses in glucose and xylose consumption phase.

    abstract:BACKGROUND:Pretreatment of lignocellulosic biomass generates a number of undesired degradation products that can inhibit microbial metabolism. Two of these compounds, the furan aldehydes 5-hydroxymethylfurfural (HMF) and 2-furaldehyde (furfural), have been shown to be an impediment for viable ethanol production. In the...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-181

    authors: Ask M,Bettiga M,Duraiswamy VR,Olsson L

    更新日期:2013-12-16 00:00:00

  • Techno-economic potential of bioethanol from bamboo in China.

    abstract:BACKGROUND:Bamboo is potentially an interesting feedstock for advanced bioethanol production in China due to its natural abundance, rapid growth, perennial nature and low management requirements. Liquid hot water (LHW) pretreatment was selected as a promising technology to enhance sugar release from bamboo lignocellulo...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-173

    authors: Littlewood J,Wang L,Turnbull C,Murphy RJ

    更新日期:2013-11-29 00:00:00

  • Real-time understanding of lignocellulosic bioethanol fermentation by Raman spectroscopy.

    abstract:BACKGROUND:A substantial barrier to commercialization of lignocellulosic ethanol production is a lack of process specific sensors and associated control strategies that are essential for economic viability. Current sensors and analytical techniques require lengthy offline analysis or are easily fouled in situ. Raman sp...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-28

    authors: Ewanick SM,Thompson WJ,Marquardt BJ,Bura R

    更新日期:2013-02-20 00:00:00

  • Use of substructure-specific carbohydrate binding modules to track changes in cellulose accessibility and surface morphology during the amorphogenesis step of enzymatic hydrolysis.

    abstract:BACKGROUND:Cellulose amorphogenesis, described as the non-hydrolytic "opening up" or disruption of a cellulosic substrate, is becoming increasingly recognized as one of the key steps in the enzymatic deconstruction of cellulosic biomass when used as a feedstock for fuels and chemicals production. Although this process ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-5-51

    authors: Gourlay K,Arantes V,Saddler JN

    更新日期:2012-07-24 00:00:00

  • Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici.

    abstract:BACKGROUND:The production of value-added chemicals alongside biofuels from lignocellulosic hydrolysates is critical for developing economically viable biorefineries. Here, the production of propionic acid (PA), a potential building block for C3-based chemicals, from corn stover hydrolysate is investigated using the nat...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0884-z

    authors: Wang X,Salvachúa D,Sànchez I Nogué V,Michener WE,Bratis AD,Dorgan JR,Beckham GT

    更新日期:2017-08-17 00:00:00

  • Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse.

    abstract:BACKGROUND:The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for their commercial application. In this study, we quantitatively compared the lignocellulolytic enzyme p...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-172

    authors: Marx IJ,van Wyk N,Smit S,Jacobson D,Viljoen-Bloom M,Volschenk H

    更新日期:2013-11-29 00:00:00

  • Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability.

    abstract:BACKGROUND:The genome of Pleurotus ostreatus, an important edible mushroom and a model ligninolytic organism of interest in lignocellulose biorefineries due to its ability to delignify agricultural wastes, was sequenced with the purpose of identifying and characterizing the enzymes responsible for lignin degradation. ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-2

    authors: Fernández-Fueyo E,Ruiz-Dueñas FJ,Martínez MJ,Romero A,Hammel KE,Medrano FJ,Martínez AT

    更新日期:2014-01-03 00:00:00

  • What cell wall components are the best indicators for Miscanthus digestibility and conversion to ethanol following variable pretreatments?

    abstract:Background:Energy crops including Miscanthus provide a storable, portable energy source which can be used to complement a wide range of products and energy generation systems. Miscanthus is predominantly used in Europe as a combustion material for electricity generation but also has the potential for biochemical conver...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1066-3

    authors: Adams JMM,Winters AL,Hodgson EM,Gallagher JA

    更新日期:2018-03-14 00:00:00

  • Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste.

    abstract:: The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its value on market was reduced and it is becoming a "waste-stream" instead of a valuable "coproduct". The developmen...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-5-48

    authors: Almeida JR,Fávaro LC,Quirino BF

    更新日期:2012-07-18 00:00:00

  • Selecting β-glucosidases to support cellulases in cellulose saccharification.

    abstract:BACKGROUND:Enzyme end-product inhibition is a major challenge in the hydrolysis of lignocellulose at a high dry matter consistency. β-glucosidases (BGs) hydrolyze cellobiose into two molecules of glucose, thereby relieving the product inhibition of cellobiohydrolases (CBHs). However, BG inhibition by glucose will event...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-105

    authors: Teugjas H,Väljamäe P

    更新日期:2013-07-24 00:00:00

  • Optimization of the dilute maleic acid pretreatment of wheat straw.

    abstract:BACKGROUND:In this study, the dilute maleic acid pretreatment of wheat straw is optimized, using pretreatment time, temperature and maleic acid concentration as design variables. A central composite design was applied to the experimental set up. The response factors used in this study are: (1) glucose benefits from imp...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-2-31

    authors: Kootstra AM,Beeftink HH,Scott EL,Sanders JP

    更新日期:2009-12-21 00:00:00

  • Production of high concentrated cellulosic ethanol by acetone/water oxidized pretreated beech wood.

    abstract:BACKGROUND:Lignocellulosic biomass is an abundant and inexpensive resource for biofuel production. Alongside its biotechnological conversion, pretreatment is essential to enable efficient enzymatic hydrolysis by making cellulose susceptible to cellulases. Wet oxidation of biomass, such as acetone/water oxidation, that ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0737-9

    authors: Katsimpouras C,Kalogiannis KG,Kalogianni A,Lappas AA,Topakas E

    更新日期:2017-02-28 00:00:00

  • Genome-wide screening of the genes required for tolerance to vanillin, which is a potential inhibitor of bioethanol fermentation, in Saccharomyces cerevisiae.

    abstract:BACKGROUND:Lignocellulosic materials are abundant and among the most important potential sources for bioethanol production. Although the pretreatment of lignocellulose is necessary for efficient saccharification and fermentation, numerous by-products, including furan derivatives, weak acids, and phenolic compounds, are...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-1-3

    authors: Endo A,Nakamura T,Ando A,Tokuyasu K,Shima J

    更新日期:2008-04-15 00:00:00

  • Allelopathy as a potential strategy to improve microalgae cultivation.

    abstract::One of the main obstacles for continuous productivity in microalgae cultivation is the presence of biological contaminants capable of eliminating large numbers of cells in a matter of days or even hours. However, a number of strategies are being used to combat and prevent contamination in microalgae cultivation. These...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-152

    authors: Bacellar Mendes LB,Vermelho AB

    更新日期:2013-10-21 00:00:00

  • Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.

    abstract:BACKGROUND:Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments. Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomer...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0757-5

    authors: Gunawan C,Xue S,Pattathil S,da Costa Sousa L,Dale BE,Balan V

    更新日期:2017-03-29 00:00:00

  • Improving the enzymatic hydrolysis of thermo-mechanical fiber from Eucalyptus urophylla by a combination of hydrothermal pretreatment and alkali fractionation.

    abstract:BACKGROUND:The recalcitrance of lignocellulosic biomass is a major limitation for its conversion into biofuels by enzymatic hydrolysis. The use of a pretreatment technology is an essential step to diminish biomass recalcitrance for bioethanol production. In this study, a two-step pretreatment using hydrothermal pretrea...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0116-8

    authors: Sun S,Cao X,Sun S,Xu F,Song X,Sun RC,Jones GL

    更新日期:2014-08-20 00:00:00

  • Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar.

    abstract:BACKGROUND:Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The effects of incubation ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-42

    authors: Wang K,Yang H,Wang W,Sun RC

    更新日期:2013-03-21 00:00:00

  • Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions.

    abstract:BACKGROUND:Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. RESULTS:In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculu...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0286-z

    authors: Rakicka M,Lazar Z,Dulermo T,Fickers P,Nicaud JM

    更新日期:2015-07-25 00:00:00

  • Facile isothermal solid acid catalyzed ionic liquid pretreatments to enhance the combined sugars production from Arundo donax Linn.

    abstract:BACKGROUND:Solid acid catalyzed inexpensive ionic liquid (IL) pretreatment is promising because of its effectiveness at decreasing biomass recalcitrance to subsequent enzymatic hydrolysis or in situ hydrolysis of carbohydrate oligomers. However, the conventional strategy was limited by the complex non-isothermal proces...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0589-8

    authors: You T,Shao L,Wang R,Zhang L,Xu F

    更新日期:2016-08-24 00:00:00

  • Comparative metagenomics of biogas-producing microbial communities from production-scale biogas plants operating under wet or dry fermentation conditions.

    abstract:BACKGROUND:Decomposition of biomass for biogas production can be practiced under wet and dry fermentation conditions. In contrast to the dry fermentation technology, wet fermentation is characterized by a high liquid content and a relatively low total solid content. In this study, the composition and functional potenti...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0193-8

    authors: Stolze Y,Zakrzewski M,Maus I,Eikmeyer F,Jaenicke S,Rottmann N,Siebner C,Pühler A,Schlüter A

    更新日期:2015-02-08 00:00:00

  • Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism.

    abstract:BACKGROUND:Non-productive cellulase adsorption onto lignin has always been deemed to negatively affect the enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding enzyme-lignin interactions is essential for the development of enzyme mixtures, the processes of lignocellulose hydrolysis, and the gene...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-38

    authors: Guo F,Shi W,Sun W,Li X,Wang F,Zhao J,Qu Y

    更新日期:2014-03-14 00:00:00

  • Bioprocessing of Stichococcus bacillaris strain siva2011.

    abstract:BACKGROUND:Globally, the development of a cost-effective long-term renewable energy infrastructure is one of the most challenging problems faced by society today. Microalgae are rich in potential biofuel substrates such as lipids, including triacylglycerols (TAGs). Some of these algae also biosynthesize small molecule ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-62

    authors: Sivakumar G,Jeong K,Lay JO Jr

    更新日期:2014-04-15 00:00:00

  • Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation.

    abstract:BACKGROUND:Furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulose biomass to monomeric sugars, but the response of acetone butanol ethanol (ABE) producing Clostridium beijerinckii NCIMB 8052 to this compound at the molecular level is unknown. To discern the effect o...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-66

    authors: Zhang Y,Ezeji TC

    更新日期:2013-05-04 00:00:00