Abstract：
BACKGROUND:In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofuel from biomass is the enzymatic saccharification. Many of the commercial cellulase enzyme cocktails, such as Spezyme(®) CP (Genencor), Acellerase™ 1000 (Genencor), and Celluclast(®) 1.5L (Novozymes), are ineffectively to release free glucose from the pretreated biomass without additional β-glucosidase. RESULTS:In this study, for the first time, a β-glucosidase DT-Bgl gene (1359 bp) was identified in the genome of Anoxybacillus sp. DT3-1, and cloned and heterologously expressed in Escherichia coli BL21. Phylogenetic analysis indicated that DT-Bgl belonged to glycosyl hydrolase (GH) family 1. The recombinant DT-Bgl was highly active on cello-oligosaccharides and p-nitrophenyl-β-d-glucopyranoside (pNPG). The DT-Bgl was purified using an Ni-NTA column, with molecular mass of 53 kDa using an SDS-PAGE analysis. It exhibited optimum activity at 70 °C and pH 8.5, and did not require any tested co-factors for activation. The K m and V max values for DT-Bgl were 0.22 mM and 923.7 U/mg, respectively, with pNPG as substrate. The DT-Bgl displayed high glucose tolerance, and retained 93 % activity in the presence of 10 M glucose. CONCLUSIONS:Anoxybacillus DT-Bgl is a novel thermostable β-glucosidase with low glucose inhibition, and converts long-chain cellodextrins to cellobiose, and further hydrolyse cellobiose to glucose. Results suggest that DT-Bgl could be useful in the development of a bioprocess for the efficient saccharification of lignocellulosic biomass.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Chan CS,Sin LL,Chan KG,Shamsir MS,Manan FA,Sani RK,Goh KMdoi
10.1186/s13068-016-0587-xsubject
Has Abstractpub_date
2016-08-22 00:00:00pages
174issue
1issn
1754-6834pii
587journal_volume
9pub_type
杂志文章abstract：BACKGROUND:Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic biomass. The commercial enzyme mixtures produced at industrial scales are not well characterized, and ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-1-18
更新日期：2008-12-23 00:00:00
abstract：UNLABELLED: BACKGROUND:Recent studies demonstrate that enzymes from the glycosyl hydrolase family 61 (GH61) show lytic polysaccharide monooxygenase (PMO) activity. Together with cellobiose dehydrogenase (CDH) an enzymatic system capable of oxidative cellulose cleavage is formed, which increases the efficiency of cellu...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-79
更新日期：2012-10-26 00:00:00
abstract：BACKGROUND:Simultaneous wastewater treatment and lipid production by oleaginous microalgae show great potential to alleviate energy shortage and environmental pollution, because they exhibit tremendous advantages over traditional activated sludge. Currently, most research on wastewater treatment by microalgal are carri...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0797-x
更新日期：2017-05-02 00:00:00
abstract：BACKGROUND:The production of biofuels and biochemicals from grass-type plant biomass requires a complete utilisation of the plant cellulose and hemicellulosic xylan via enzymatic degradation to their constituent monosaccharides. Generally, physical and/or thermochemical pretreatments are performed to enable access for ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0519-9
更新日期：2016-05-18 00:00:00
abstract：BACKGROUND:The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strai...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-014-0154-2
更新日期：2014-10-18 00:00:00
abstract：BACKGROUND:Development of biofuels is considered as one of the important ways to replace conventional fossil energy and mitigate climate change. However, rapid increase of biofuel production could cause other environmental concerns in China such as water stress. This study is intended to evaluate the life-cycle water f...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0778-0
更新日期：2017-04-18 00:00:00
abstract：UNLABELLED: BACKGROUND:The recalcitrance of lignocellulosic cell wall biomass to deconstruction varies greatly in angiosperms, yet the source of this variation remains unclear. Here, in eight genotypes of short rotation coppice willow (Salix sp.) variability of the reaction wood (RW) response and the impact of this va...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-83
更新日期：2012-11-22 00:00:00
abstract：Background:Irpex lacteus is one of the most potent white rot fungi for biological pretreatment of lignocellulose for second biofuel production. To elucidate the underlying molecular mechanism involved in lignocellulose deconstruction, genomic and transcriptomic analyses were carried out for I. lacteus CD2 grown in subm...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1060-9
更新日期：2018-03-02 00:00:00
abstract：BACKGROUND:The diverse microbial communities in agricultural biogas fermenters are assumed to be well adapted for the anaerobic transformation of plant biomass to methane. Compared to natural systems, biogas reactors are limited in their hydrolytic potential. The reasons for this are not understood. RESULTS:In this pa...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0534-x
更新日期：2016-06-07 00:00:00
abstract：BACKGROUND:Improvement in the performance of eukaryotic microalgae for biofuel and bioproduct production is largely dependent on characterization of metabolic mechanisms within the cell. The marine diatom Cyclotella cryptica, which was originally identified in the Aquatic Species Program, is a promising strain of micro...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0670-3
更新日期：2016-11-25 00:00:00
abstract：BACKGROUND:The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes. RESULTS:Trichosporon mycotoxinivorans CLA2 is bioemulsifier-producing yeast strain isolated ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-29
更新日期：2012-05-06 00:00:00
abstract：:[This retracts the article DOI: 10.1186/s13068-017-0920-z.]. ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章,撤回出版物
doi：10.1186/s13068-018-1086-z
更新日期：2018-04-06 00:00:00
abstract：UNLABELLED: BACKGROUND:The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two conversion systems (simultaneous saccharification and fermentation using yeast and fungal ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-49
更新日期：2012-07-19 00:00:00
abstract：BACKGROUND:Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments. Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomer...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0757-5
更新日期：2017-03-29 00:00:00
abstract：Background:Microalgae are attracting much attention as a promising feedstock for renewable energy production, while simultaneously providing environmental benefits. So far, comparison studies for microalgae selection for this purpose were mainly based on data obtained from batch cultures, where the lipid content and th...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1461-4
更新日期：2019-05-11 00:00:00
abstract：Background:The recalcitrance of lignocellulosic biomass offers a series of challenges for biochemical processing into biofuels and bio-products. For the first time, we address these challenges with a biomimetic system via a mild yet rapid Fenton reaction and lignocellulose-degrading bacterial strain Cupriavidus basilen...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1035-x
更新日期：2018-02-08 00:00:00
abstract：Background:Black soldier fly (BSF, Hermetia illucens L.) can efficiently degrade organic wastes and transform into a high fat containing insect biomass that could be used as feedstock for biodiesel production. Meanwhile, the molecular regulatory basis of fat accumulation by BSF is still unclear; it is necessary to iden...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1531-7
更新日期：2019-08-09 00:00:00
abstract：BACKGROUND:A substantial barrier to commercialization of lignocellulosic ethanol production is a lack of process specific sensors and associated control strategies that are essential for economic viability. Current sensors and analytical techniques require lengthy offline analysis or are easily fouled in situ. Raman sp...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-28
更新日期：2013-02-20 00:00:00
abstract：BACKGROUND:Low cost of raw materials and good process yields are necessary for future lignocellulosic biomass biorefineries to be sustainable and profitable. A low cost feedstock will be diverse, changing as a function of seasonality and price and will most likely be available from multiple sources to the biorefinery. ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0414-9
更新日期：2015-12-24 00:00:00
abstract：BACKGROUND:Solid acid catalyzed inexpensive ionic liquid (IL) pretreatment is promising because of its effectiveness at decreasing biomass recalcitrance to subsequent enzymatic hydrolysis or in situ hydrolysis of carbohydrate oligomers. However, the conventional strategy was limited by the complex non-isothermal proces...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0589-8
更新日期：2016-08-24 00:00:00
abstract：BACKGROUND:The recent development of improved enzymes and pentose-using yeast for cellulosic ethanol processes calls for new attention to the lignocellulose pretreatment step. This study assessed the influence of pretreatment pH, temperature, and time, and their interactions on the enzymatic glucose and xylose yields f...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-4-11
更新日期：2011-05-13 00:00:00
abstract：Background:For bioethanol production from lignocellulosic biomass, phenolics derived from pretreatment have been generally considered as highly inhibitory towards enzymatic hydrolysis and fermentation. As phenolics are produced from lignin degradation during pretreatment, it is likely that the pretreatment will exert a...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01686-z
更新日期：2020-03-11 00:00:00
abstract：BACKGROUND:Although numerous studies have used wastewater as substitutes to cultivate microalgae, most of them obtained weaker algal viability than standard media. Some studies demonstrated a promotion of phytohormones on algal growth in standard media. For exploiting a strategy to improve algal biomass accumulation in...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0759-3
更新日期：2017-03-24 00:00:00
abstract：Background:Cellulolytic enzymes produced by Trichoderma reesei are widely used for the industrial production of biofuels and chemicals from lignocellulose. We speculated that intracellular pH during the fermentation process can affect cellulase induction. Results:In this study, two H+-ATPase genes, tre76238 and tre787...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1536-2
更新日期：2019-08-13 00:00:00
abstract：Background:Beer is the most popular alcoholic beverage worldwide. In the manufacture of beer, various by-products and residues are generated, and the most abundant (85% of total by-products) are spent grains. Thanks to its high (hemi)cellulose content (about 50% w/w dry weight), this secondary raw material is attractiv...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1383-1
更新日期：2019-03-05 00:00:00
abstract：Background:Energy crops including Miscanthus provide a storable, portable energy source which can be used to complement a wide range of products and energy generation systems. Miscanthus is predominantly used in Europe as a combustion material for electricity generation but also has the potential for biochemical conver...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1066-3
更新日期：2018-03-14 00:00:00
abstract：BACKGROUND:Oxygenic photosynthetic microorganisms such as cyanobacteria and microalgae have attracted attention as an alternative carbon source for the next generation of biofuels. Glycogen abundantly accumulated in cyanobacteria is a promising feedstock which can be converted to ethanol through saccharification and fe...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-7-88
更新日期：2014-06-11 00:00:00
abstract：BACKGROUND:Oleaginous microorganisms are attractive feedstock for production of liquid biofuels. Direct hydrothermal liquefaction (HTL) is an efficient route that converts whole, wet biomass into an energy-dense liquid fuel precursor, called 'biocrude'. HTL represents a promising alternative to conventional lipid extra...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0345-5
更新日期：2015-10-13 00:00:00
abstract：Background:In the enzymatic conversion of biomass, it becomes an important issue to efficiently and cost-effectively degrade cellulose into fermentable glucose. β-Glucosidase (Bgluc), an essential member of cellulases, plays a critical role in cellulosic biomass degradation. The difficulty in improving the stability of...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1497-5
更新日期：2019-06-24 00:00:00
abstract：:Microalgae hold great promises as sustainable cellular factories for the production of alternative fuels, feeds, and biopharmaceuticals for human health. While the biorefinery approach for fuels along with the coproduction of high-value compounds with industrial, therapeutic, or nutraceutical applications have the pot...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章,评审
doi：10.1186/s13068-019-1635-0
更新日期：2019-12-23 00:00:00
