Abstract:
BACKGROUND:The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC 7002. RESULTS:Optimal growth conditions for co-cultivation of C. reinhardtii with wild-type and mutant strains of Synechococcus sp. 7002 were established. In co-culture, acetate produced by a glycogen synthase knockout mutant of Synechococcus sp. PCC 7002 was able to support the growth of a lipid-accumulating mutant strain of C. reinhardtii defective in starch production. Encapsulation of Synechococcus sp. PCC 7002 using an alginate matrix was successfully employed in co-cultures to limit growth and maintain the stability. CONCLUSIONS:The ability of immobilized strains of the cyanobacterium Synechococcus sp. PCC 7002 to produce acetate at a level adequate to support the growth of lipid-accumulating strains of C. reinhartdii offers a potentially practical, photosynthetic alternative to providing exogenous acetate into growth media.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Therien JB,Zadvornyy OA,Posewitz MC,Bryant DA,Peters JWdoi
10.1186/s13068-014-0154-2subject
Has Abstractpub_date
2014-10-18 00:00:00pages
154issue
1issn
1754-6834pii
154journal_volume
7pub_type
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