A bionic system with Fenton reaction and bacteria as a model for bioprocessing lignocellulosic biomass.

Abstract:

Background:The recalcitrance of lignocellulosic biomass offers a series of challenges for biochemical processing into biofuels and bio-products. For the first time, we address these challenges with a biomimetic system via a mild yet rapid Fenton reaction and lignocellulose-degrading bacterial strain Cupriavidus basilensis B-8 (here after B-8) to pretreat the rice straw (RS) by mimicking the natural fungal invasion process. Here, we also elaborated the mechanism through conducting a systematic study of physicochemical changes before and after pretreatment. Results:After synergistic Fenton and B-8 pretreatment, the reducing sugar yield was increased by 15.6-56.6% over Fenton pretreatment alone and 2.7-5.2 times over untreated RS (98 mg g-1). Morphological analysis revealed that pretreatment changed the surface morphology of the RS, and the increase in roughness and hydrophilic sites enhanced lignocellulose bioavailability. Chemical components analyses showed that B-8 removed part of the lignin and hemicellulose which caused the cellulose content to increase. In addition, the important chemical modifications also occurred in lignin, 2D NMR analysis of the lignin in residues indicated that the Fenton pretreatment caused partial depolymerization of lignin mainly by cleaving the β-O-4 linkages and by demethoxylation to remove the syringyl (S) and guaiacyl (G) units. B-8 could depolymerize amount of the G units by cleaving the β-5 linkages that interconnect the lignin subunits. Conclusions:A biomimetic system with a biochemical Fenton reaction and lignocellulose-degrading bacteria was confirmed to be able for the pretreatment of RS to enhance enzymatic hydrolysis under mild conditions. The high digestibility was attributed to the destruction of the lignin structure, partial hydrolysis of the hemicellulose and partial surface oxidation of the cellulose. The mechanism of synergistic Fenton and B-8 pretreatment was also explored to understand the change in the RS and the bacterial effects on enzymatic hydrolysis. Furthermore, this biomimetic system offers new insights into the pretreatment of lignocellulosic biomass.

journal_name

Biotechnol Biofuels

authors

Zhang K,Si M,Liu D,Zhuo S,Liu M,Liu H,Yan X,Shi Y

doi

10.1186/s13068-018-1035-x

subject

Has Abstract

pub_date

2018-02-08 00:00:00

pages

31

issn

1754-6834

pii

1035

journal_volume

11

pub_type

杂志文章
  • The impact of alterations in lignin deposition on cellulose organization of the plant cell wall.

    abstract:BACKGROUND:Coordination of synthesis and assembly of the polymeric components of cell walls is essential for plant growth and development. Given the degree of co-mingling and cross-linking among cell wall components, cellulose organization must be dependent on the organization of other polymers such as lignin. Here we ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0540-z

    authors: Liu J,Kim JI,Cusumano JC,Chapple C,Venugopalan N,Fischetti RF,Makowski L

    更新日期:2016-06-17 00:00:00

  • Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability.

    abstract:BACKGROUND:The genome of Pleurotus ostreatus, an important edible mushroom and a model ligninolytic organism of interest in lignocellulose biorefineries due to its ability to delignify agricultural wastes, was sequenced with the purpose of identifying and characterizing the enzymes responsible for lignin degradation. ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-2

    authors: Fernández-Fueyo E,Ruiz-Dueñas FJ,Martínez MJ,Romero A,Hammel KE,Medrano FJ,Martínez AT

    更新日期:2014-01-03 00:00:00

  • Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products.

    abstract::Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorization of lignin to chemicals and fuels. However, due to the structural heterogeneity of lignin, depolymerization ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章,评审

    doi:10.1186/s13068-019-1376-0

    authors: Xu Z,Lei P,Zhai R,Wen Z,Jin M

    更新日期:2019-02-15 00:00:00

  • Freshwater microalgae harvested via flocculation induced by pH decrease.

    abstract:BACKGROUND:Recent studies have demonstrated that microalga has been widely regarded as one of the most promising raw materials of biofuels. However, lack of an economical, efficient and convenient method to harvest microalgae is a bottleneck to boost their full-scale application. Many methods of harvesting microalgae, ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-98

    authors: Liu J,Zhu Y,Tao Y,Zhang Y,Li A,Li T,Sang M,Zhang C

    更新日期:2013-07-09 00:00:00

  • High concentrations of dried sorghum stalks as a biomass feedstock for single cell oil production by Rhodosporidium toruloides.

    abstract:BACKGROUND:Environmental crisis and concerns for energy security have made the research for renewable fuels that will substitute the usage of fossil fuels an important priority. Biodiesel is a potential substitute for petroleum, but its feasibility is hindered by the utilization of edible vegetable oil as raw material,...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0190-y

    authors: Matsakas L,Bonturi N,Miranda EA,Rova U,Christakopoulos P

    更新日期:2015-01-22 00:00:00

  • Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature.

    abstract:BACKGROUND:Simultaneous saccharification and fermentation (SSF) is a promising process for bioconversion of lignocellulosic biomass. High glucan loading for hydrolysis and fermentation is an efficient approach to reduce the capital costs for bio-based products production. The SSF of steam-exploded corn stover (SECS) fo...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0167-x

    authors: Liu ZH,Qin L,Zhu JQ,Li BZ,Yuan YJ

    更新日期:2014-12-04 00:00:00

  • Solid-state NMR of unlabeled plant cell walls: high-resolution structural analysis without isotopic enrichment.

    abstract:BACKGROUND:Multidimensional solid-state nuclear magnetic resonance (ssNMR) spectroscopy has emerged as an indispensable technique for resolving polymer structure and intermolecular packing in primary and secondary plant cell walls. Isotope (13C) enrichment provides feasible sensitivity for measuring 2D/3D correlation s...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-020-01858-x

    authors: Zhao W,Kirui A,Deligey F,Mentink-Vigier F,Zhou Y,Zhang B,Wang T

    更新日期:2021-01-07 00:00:00

  • Effects of lignin and surfactant on adsorption and hydrolysis of cellulases on cellulose.

    abstract:BACKGROUND:Considerable works have been reported concerning the obstruction of enzymatic hydrolysis efficiency by lignin. However, there is a lack of information about the influence of lignin on the adsorption of cellulases on cellulose, along with the hydrolytic activity of the cellulases adsorbed on lignin. In additi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0434-0

    authors: Li Y,Sun Z,Ge X,Zhang J

    更新日期:2016-01-26 00:00:00

  • Synergistic effects of mixing hybrid poplar and wheat straw biomass for bioconversion processes.

    abstract:BACKGROUND:Low cost of raw materials and good process yields are necessary for future lignocellulosic biomass biorefineries to be sustainable and profitable. A low cost feedstock will be diverse, changing as a function of seasonality and price and will most likely be available from multiple sources to the biorefinery. ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0414-9

    authors: Vera RM,Bura R,Gustafson R

    更新日期:2015-12-24 00:00:00

  • Evaluation of ammonia fibre expansion (AFEX) pretreatment for enzymatic hydrolysis of switchgrass harvested in different seasons and locations.

    abstract:BACKGROUND:When producing biofuels from dedicated feedstock, agronomic factors such as harvest time and location can impact the downstream production. Thus, this paper studies the effectiveness of ammonia fibre expansion (AFEX) pretreatment on two harvest times (July and October) and ecotypes/locations (Cave-in-Rock (C...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-3-1

    authors: Bals B,Rogers C,Jin M,Balan V,Dale B

    更新日期:2010-01-04 00:00:00

  • From grass to gas: microbiome dynamics of grass biomass acidification under mesophilic and thermophilic temperatures.

    abstract:BACKGROUND:Separating acidification and methanogenic steps in anaerobic digestion processes can help to optimize the process and contribute to producing valuable sub-products such as methane, hydrogen and organic acids. However, the full potential of this technology has not been fully explored yet. To assess the underl...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0859-0

    authors: Abendroth C,Simeonov C,Peretó J,Antúnez O,Gavidia R,Luschnig O,Porcar M

    更新日期:2017-07-03 00:00:00

  • Single-step bioconversion of lignocellulose to hydrogen using novel moderately thermophilic bacteria.

    abstract:BACKGROUND:Consolidated bioprocessing (CBP) of lignocellulosic biomass to hydrogen offers great potential for lower cost and higher efficiency compared to processes featuring dedicated cellulase production. Current studies on CBP-based hydrogen production mainly focus on using the thermophilic cellulolytic bacterium Cl...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-82

    authors: Cao GL,Zhao L,Wang AJ,Wang ZY,Ren NQ

    更新日期:2014-06-03 00:00:00

  • Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002.

    abstract:BACKGROUND:The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strai...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0154-2

    authors: Therien JB,Zadvornyy OA,Posewitz MC,Bryant DA,Peters JW

    更新日期:2014-10-18 00:00:00

  • Improvement of catalytic performance of lignin peroxidase for the enhanced degradation of lignocellulose biomass based on the imbedded electron-relay in long-range electron transfer route.

    abstract:BACKGROUND:Although lignin peroxidase is claimed as a key enzyme in enzyme-catalyzed lignin degradation, in vitro enzymatic degradation of lignin was not easily observed in lab-scale experiments. It implies that other factors may hinder the enzymatic degradation of lignin. Irreversible interaction between phenolic comp...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0664-1

    authors: Pham LT,Kim SJ,Kim YH

    更新日期:2016-11-15 00:00:00

  • Remembering Mary (1917 to 2008): editorial introduction to the thematic series on the life and lifework of Mary Mandels, first lady of cellulase research.

    abstract::Editorial introduction to the thematic series on the life and lifework of Mary Mandels. ...

    journal_title:Biotechnology for biofuels

    pub_type: 社论

    doi:10.1186/1754-6834-2-23

    authors: Bayer EA

    更新日期:2009-09-01 00:00:00

  • Techno-economic evaluation of stillage treatment with anaerobic digestion in a softwood-to-ethanol process.

    abstract:BACKGROUND:Replacing the energy-intensive evaporation of stillage by anaerobic digestion is one way of decreasing the energy demand of the lignocellulosic biomass to the ethanol process. The biogas can be upgraded and sold as transportation fuel, injected directly into the gas grid or be incinerated on-site for combine...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-3-21

    authors: Barta Z,Reczey K,Zacchi G

    更新日期:2010-09-15 00:00:00

  • Production of 2,3-butanediol from glucose and cassava hydrolysates by metabolically engineered industrial polyploid Saccharomyces cerevisiae.

    abstract:Background:2,3-Butanediol (2,3-BDO) is a valuable chemical for industrial applications. Bacteria can produce 2,3-BDO with a high productivity, though most of their classification as pathogens makes them undesirable for the industrial-scale production. Though Saccharomyces cerevisiae (GRAS microorganism) was engineered ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1545-1

    authors: Lee YG,Seo JH

    更新日期:2019-08-29 00:00:00

  • Metabolic engineering of a fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for photoautotrophic production of succinic acid.

    abstract:Background:Cyanobacteria, a group of photosynthetic prokaryotes, are being increasingly explored for direct conversion of carbon dioxide to useful chemicals. However, efforts to engineer these photoautotrophs have resulted in low product titers. This may be ascribed to the bottlenecks in metabolic pathways, which need ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-020-01727-7

    authors: Sengupta S,Jaiswal D,Sengupta A,Shah S,Gadagkar S,Wangikar PP

    更新日期:2020-05-18 00:00:00

  • Presence does not imply activity: DNA and RNA patterns differ in response to salt perturbation in anaerobic digestion.

    abstract:BACKGROUND:The microbial community in anaerobic digestion is mainly monitored by means of DNA-based methods. This may lead to incorrect interpretation of the community parameters, because microbial abundance does not necessarily reflect activity. In this research, the difference between microbial community response on ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0652-5

    authors: De Vrieze J,Regueiro L,Props R,Vilchez-Vargas R,Jáuregui R,Pieper DH,Lema JM,Carballa M

    更新日期:2016-11-09 00:00:00

  • In situ label-free imaging of hemicellulose in plant cell walls using stimulated Raman scattering microscopy.

    abstract:BACKGROUND:Plant hemicellulose (largely xylan) is an excellent feedstock for renewable energy production and second only to cellulose in abundance. Beyond a source of fermentable sugars, xylan constitutes a critical polymer in the plant cell wall, where its precise role in wall assembly, maturation, and deconstruction ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0669-9

    authors: Zeng Y,Yarbrough JM,Mittal A,Tucker MP,Vinzant TB,Decker SR,Himmel ME

    更新日期:2016-11-22 00:00:00

  • Transparent polyvinyl-alcohol cryogel as immobilisation matrix for continuous biohydrogen production by phototrophic bacteria.

    abstract:Background:Phototrophic purple non-sulfur bacteria (PNSB) have gained attention for their ability to produce a valuable clean energy source in the form biohydrogen via photofermentation of a wide variety of organic wastes. For maturation of these phototrophic bioprocesses towards commercial feasibility, development of ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-020-01743-7

    authors: du Toit JP,Pott RWM

    更新日期:2020-06-09 00:00:00

  • Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering.

    abstract:Background:Astaxanthin is a natural carotenoid pigment with tremendous antioxidant activity and great commercial value. Microbial production of astaxanthin via metabolic engineering has become a promising alternative. Although great efforts have been conducted by tuning the heterologous modules and precursor pools, the...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1227-4

    authors: Jin J,Wang Y,Yao M,Gu X,Li B,Liu H,Ding M,Xiao W,Yuan Y

    更新日期:2018-08-23 00:00:00

  • Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism.

    abstract:BACKGROUND:Non-productive cellulase adsorption onto lignin has always been deemed to negatively affect the enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding enzyme-lignin interactions is essential for the development of enzyme mixtures, the processes of lignocellulose hydrolysis, and the gene...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-38

    authors: Guo F,Shi W,Sun W,Li X,Wang F,Zhao J,Qu Y

    更新日期:2014-03-14 00:00:00

  • Chemical and structural changes associated with Cu-catalyzed alkaline-oxidative delignification of hybrid poplar.

    abstract:BACKGROUND:Alkaline hydrogen peroxide pretreatment catalyzed by Cu(II) 2,2'-bipyridine complexes has previously been determined to substantially improve the enzymatic hydrolysis of woody plants including hybrid poplar as a consequence of moderate delignification. In the present work, cell wall morphological and lignin ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0300-5

    authors: Li Z,Bansal N,Azarpira A,Bhalla A,Chen CH,Ralph J,Hegg EL,Hodge DB

    更新日期:2015-08-20 00:00:00

  • Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay.

    abstract:UNLABELLED: BACKGROUND:Recent studies demonstrate that enzymes from the glycosyl hydrolase family 61 (GH61) show lytic polysaccharide monooxygenase (PMO) activity. Together with cellobiose dehydrogenase (CDH) an enzymatic system capable of oxidative cellulose cleavage is formed, which increases the efficiency of cellu...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-5-79

    authors: Kittl R,Kracher D,Burgstaller D,Haltrich D,Ludwig R

    更新日期:2012-10-26 00:00:00

  • Unraveling the microbiome of a thermophilic biogas plant by metagenome and metatranscriptome analysis complemented by characterization of bacterial and archaeal isolates.

    abstract:BACKGROUND:One of the most promising technologies to sustainably produce energy and to mitigate greenhouse gas emissions from combustion of fossil energy carriers is the anaerobic digestion and biomethanation of organic raw material and waste towards biogas by highly diverse microbial consortia. In this context, the mi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0581-3

    authors: Maus I,Koeck DE,Cibis KG,Hahnke S,Kim YS,Langer T,Kreubel J,Erhard M,Bremges A,Off S,Stolze Y,Jaenicke S,Goesmann A,Sczyrba A,Scherer P,König H,Schwarz WH,Zverlov VV,Liebl W,Pühler A,Schlüter A,Klocke M

    更新日期:2016-08-11 00:00:00

  • Selecting β-glucosidases to support cellulases in cellulose saccharification.

    abstract:BACKGROUND:Enzyme end-product inhibition is a major challenge in the hydrolysis of lignocellulose at a high dry matter consistency. β-glucosidases (BGs) hydrolyze cellobiose into two molecules of glucose, thereby relieving the product inhibition of cellobiohydrolases (CBHs). However, BG inhibition by glucose will event...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-105

    authors: Teugjas H,Väljamäe P

    更新日期:2013-07-24 00:00:00

  • Bioflocculants' production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest.

    abstract:Background:Bioflocculation has been developed as a cost-effective and environment-friendly method to harvest multiple microalgae. However, the high production cost of bioflocculants makes it difficult to scale up. In the current study, low-cost bioflocculants were produced from untreated corn stover by a biomass-degrad...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0987-6

    authors: Guo H,Hong C,Zheng B,Lu F,Jiang D,Qin W

    更新日期:2017-12-20 00:00:00

  • From wheat straw to bioethanol: integrative analysis of a separate hydrolysis and co-fermentation process with implemented enzyme production.

    abstract:BACKGROUND:Lignocellulosic ethanol has a high potential as renewable energy source. In recent years, much research effort has been spent to optimize parameters involved in the production process. Despite that, there is still a lack of comprehensive studies on process integration. Single parameters and process configura...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0232-0

    authors: Novy V,Longus K,Nidetzky B

    更新日期:2015-03-18 00:00:00

  • Reduced use of phosphorus and water in sequential dark fermentation and anaerobic digestion of wheat straw and the application of ensiled steam-pretreated lucerne as a macronutrient provider in anaerobic digestion.

    abstract:Background:Current EU directives demand increased use of renewable fuels in the transportation sector but restrict governmental support for production of biofuels produced from crops. The use of intercropped lucerne and wheat may comply with the directives. In the current study, the combination of ensiled lucerne (Medi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1280-z

    authors: Byrne E,Kovacs K,van Niel EWJ,Willquist K,Svensson SE,Kreuger E

    更新日期:2018-10-11 00:00:00