Abstract：
Background:Biodiesel and flavor compound production using enzymatic transesterification by microbial lipases provides mild reaction conditions and low energy cost compared to the chemical process. SGNH-type lipases are very effective catalysts for enzymatic transesterification due to their high reaction rate, great stability, relatively small size for convenient genetic manipulations, and ease of immobilization. Hence, it is highly important to identify novel SGNH-type lipases with high catalytic efficiencies and good stabilities. Results:A promiscuous cold-adapted SGNH-type lipase (HaSGNH1) from Halocynthiibacter arcticus was catalytically characterized and functionally explored. HaSGNH1 displayed broad substrate specificity that included tert-butyl acetate, glucose pentaacetate, and p-nitrophenyl esters with excellent stability and high efficiency. Important amino acids (N83, M86, R87, F131, and I173F) around the substrate-binding pocket were shown to be responsible for catalytic activity, substrate specificity, and reaction kinetics. Moreover, immobilized HaSGNH1 was used to produce high yields of butyl and oleic esters. Conclusions:This work provides a molecular understanding of substrate specificities, catalytic regulation, immobilization, and industrial applications of a promiscuous cold-adapted SGNH-type lipase (HaSGNH1) from H. arcticus. This is the first analysis on biodiesel and flavor synthesis using a cold-adapted halophilic SGNH-type lipase from a Halocynthiibacter species.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Le LTHL,Yoo W,Jeon S,Lee C,Kim KK,Lee JH,Kim TDdoi
10.1186/s13068-020-01696-xsubject
Has Abstractpub_date
2020-03-16 00:00:00pages
55issn
1754-6834pii
1696journal_volume
13pub_type
杂志文章abstract：BACKGROUND:Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The effects of incubation ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-42
更新日期：2013-03-21 00:00:00
abstract：BACKGROUND:Brown algae are promising feedstocks for biofuel production with inherent advantages of no structural lignin, high growth rate, and no competition for land and fresh water. However, it is difficult for one microorganism to convert all components of brown algae with different oxidoreduction potentials to etha...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0494-1
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-2-25
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1427-6
更新日期：2019-04-23 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-98
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0589-8
更新日期：2016-08-24 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-31
更新日期：2012-07-11 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01815-8
更新日期：2020-10-20 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0669-9
更新日期：2016-11-22 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-105
更新日期：2013-07-24 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0367-z
更新日期：2015-11-21 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1619-0
更新日期：2019-12-05 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1042-y
更新日期：2018-02-21 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1641-2
更新日期：2020-01-22 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01672-5
更新日期：2020-03-05 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1287-5
更新日期：2018-10-16 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1566-9
更新日期：2019-09-16 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1388-9
更新日期：2019-03-09 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1545-1
更新日期：2019-08-29 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-2-31
更新日期：2009-12-21 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0631-x
更新日期：2016-10-14 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0396-7
更新日期：2015-12-21 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0923-9
更新日期：2017-10-11 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0961-3
更新日期：2017-11-30 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0573-3
更新日期：2016-07-26 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-1-3
更新日期：2008-04-15 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1436-5
更新日期：2019-04-30 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1442-7
更新日期：2019-05-03 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0747-7
更新日期：2017-03-09 00:00:00
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journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1300-z
更新日期：2018-11-09 00:00:00
