Microbial effects of part-stream low-frequency ultrasonic pretreatment on sludge anaerobic digestion as revealed by high-throughput sequencing-based metagenomics and metatranscriptomics.

Abstract:

Background:Part-stream low-frequency ultrasound (LFUS) was one of the common practices for sludge disintegration in full-scale anaerobic digestion (AD) facilities. However, the effectiveness of part-stream LFUS treatment and its effect on AD microbiome have not been fully elucidated. Methods:Here we testified the effectiveness of part-stream LFUS pretreatment by treating only a fraction of feed sludge (23% and 33% total solid of the feed sludge) with 20 Hz LFUS for 70 s. State-of-the-art metagenomic and metatranscriptomic analysis was used to investigate the microbial process underpinning the enhanced AD performance by part-stream LFUS pretreatment. Results:By pretreating 33% total solid of the feed sludge, methane yield was increased by 36.5%, while the volatile solid reduction ratio remained unchanged. RNA-seq of the microbiome at stable stage showed that the continuous dosage of easy-degradable LFUS-pretreated feed sludge had gradually altered the microbial community by selecting Bacteroidales hydrolyzer with greater metabolic capability to hydrolyze cellulosic biomass without substrate attachment. Meanwhile, Thermotogales with excellent cell mobility for nutrient capturing was highly active within the community. Foremost proportion of the methanogenesis was contributed by the dominant Methanomicrobiales via carbon dioxide reduction. More interestingly, a perceivable proportion of the reverse electron flow of the community was input from Methanoculleus species other than syntrophic acetate-oxidizing bacteria. In addition, metagenomic binning retrieved several interesting novel metagenomic-assembled genomes (MAGs): MAG-bin6 of Alistipes shahii showed exceptional transcriptional activities towards protein degradation and MAG-bin11 of Candidatus Cloacimonetes with active cellulolytic GH74 gene detected. Conclusions:In summary, despite the unchanged sludge digestibility, the applied part-stream LFUS pretreatment strategy was robust in adjusting the microbial pathways towards more effective substrate conversion enabled by free-living hydrolyser and beta-oxidation-capable methanogens.

journal_name

Biotechnol Biofuels

authors

Xia Y,Yang C,Zhang T

doi

10.1186/s13068-018-1042-y

subject

Has Abstract

pub_date

2018-02-21 00:00:00

pages

47

issn

1754-6834

pii

1042

journal_volume

11

pub_type

杂志文章
  • The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973.

    abstract:Background:Cyanobacteria have shown promising potential for the production of various biofuels and chemical feedstocks. Synechococcus elongatus UTEX 2973 is a fast-growing strain with pronounced tolerance to high temperatures and illumination. Hence, this strain appears to be ideal for the development of photosynthetic...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1215-8

    authors: Tan X,Hou S,Song K,Georg J,Klähn S,Lu X,Hess WR

    更新日期:2018-08-04 00:00:00

  • Evolutionary engineering in Saccharomyces cerevisiae reveals a TRK1-dependent potassium influx mechanism for propionic acid tolerance.

    abstract:Background:Propionic acid (PA), a key platform chemical produced as a by-product during petroleum refining, has been widely used as a food preservative and an important chemical intermediate in many industries. Microbial PA production through engineering yeast as a cell factory is a potentially sustainable alternative ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1427-6

    authors: Xu X,Williams TC,Divne C,Pretorius IS,Paulsen IT

    更新日期:2019-04-23 00:00:00

  • Peptide-mediated microalgae harvesting method for efficient biofuel production.

    abstract:BACKGROUND:Production of biofuels from microalgae has been recognized to be a promising route for a sustainable energy supply. However, the microalgae harvesting process is a bottleneck for industrialization because it is energy intensive. Thus, by displaying interactive protein factors on the cell wall, oleaginous mic...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0406-9

    authors: Maeda Y,Tateishi T,Niwa Y,Muto M,Yoshino T,Kisailus D,Tanaka T

    更新日期:2016-01-13 00:00:00

  • Improving carbohydrate and starch accumulation in Chlorella sp. AE10 by a novel two-stage process with cell dilution.

    abstract:BACKGROUND:Microalgae are highly efficient cellular factories that capture CO2 and are also alternative feedstock for biofuel production. Carbohydrates, proteins, and lipids are major biochemical components in microalgae. Carbohydrates or starch in microalgae are possible substrates in yeast fermentation for biofuel pr...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0753-9

    authors: Cheng D,Li D,Yuan Y,Zhou L,Li X,Wu T,Wang L,Zhao Q,Wei W,Sun Y

    更新日期:2017-03-24 00:00:00

  • Genome-wide screening of the genes required for tolerance to vanillin, which is a potential inhibitor of bioethanol fermentation, in Saccharomyces cerevisiae.

    abstract:BACKGROUND:Lignocellulosic materials are abundant and among the most important potential sources for bioethanol production. Although the pretreatment of lignocellulose is necessary for efficient saccharification and fermentation, numerous by-products, including furan derivatives, weak acids, and phenolic compounds, are...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-1-3

    authors: Endo A,Nakamura T,Ando A,Tokuyasu K,Shima J

    更新日期:2008-04-15 00:00:00

  • Correction to: Pelagibaca bermudensis promotes biofuel competence of Tetraselmis striata in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity.

    abstract::[This corrects the article DOI: 10.1186/s13068-018-1097-9.]. ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章,已发布勘误

    doi:10.1186/s13068-018-1185-x

    authors: Patidar SK,Kim SH,Kim JH,Park J,Park BS,Han MS

    更新日期:2018-07-03 00:00:00

  • Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance.

    abstract:Background:Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by converting it to xylulose. For this, the type II xylose isomerase ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1643-0

    authors: Lee M,Rozeboom HJ,Keuning E,de Waal P,Janssen DB

    更新日期:2020-01-11 00:00:00

  • Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability.

    abstract:Background:In the enzymatic conversion of biomass, it becomes an important issue to efficiently and cost-effectively degrade cellulose into fermentable glucose. β-Glucosidase (Bgluc), an essential member of cellulases, plays a critical role in cellulosic biomass degradation. The difficulty in improving the stability of...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1497-5

    authors: Zhou Y,Li X,Yan D,Addai Peprah F,Ji X,Fletcher EE,Wang Y,Wang Y,Gu J,Lin F,Shi H

    更新日期:2019-06-24 00:00:00

  • Combined resistance to oxidative stress and reduced antenna size enhance light-to-biomass conversion efficiency in Chlorella vulgaris cultures.

    abstract:Background:Microalgae are efficient producers of lipid-rich biomass, making them a key component in developing a sustainable energy source, and an alternative to fossil fuels. Chlorella species are of special interest because of their fast growth rate in photobioreactors. However, biological constraints still cast a si...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1566-9

    authors: Dall'Osto L,Cazzaniga S,Guardini Z,Barera S,Benedetti M,Mannino G,Maffei ME,Bassi R

    更新日期:2019-09-16 00:00:00

  • Electrolytic extraction drives volatile fatty acid chain elongation through lactic acid and replaces chemical pH control in thin stillage fermentation.

    abstract:BACKGROUND:Volatile fatty acids (VFA) are building blocks for the chemical industry. Sustainable, biological production is constrained by production and recovery costs, including the need for intensive pH correction. Membrane electrolysis has been developed as an in situ extraction technology tailored to the direct rec...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0396-7

    authors: Andersen SJ,Candry P,Basadre T,Khor WC,Roume H,Hernandez-Sanabria E,Coma M,Rabaey K

    更新日期:2015-12-21 00:00:00

  • Leveraging transcription factors to speed cellobiose fermentation by Saccharomyces cerevisiae.

    abstract:BACKGROUND:Saccharomyces cerevisiae, a key organism used for the manufacture of renewable fuels and chemicals, has been engineered to utilize non-native sugars derived from plant cell walls, such as cellobiose and xylose. However, the rates and efficiencies of these non-native sugar fermentations pale in comparison wit...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0126-6

    authors: Lin Y,Chomvong K,Acosta-Sampson L,Estrela R,Galazka JM,Kim SR,Jin YS,Cate JH

    更新日期:2014-08-27 00:00:00

  • Use of substructure-specific carbohydrate binding modules to track changes in cellulose accessibility and surface morphology during the amorphogenesis step of enzymatic hydrolysis.

    abstract:BACKGROUND:Cellulose amorphogenesis, described as the non-hydrolytic "opening up" or disruption of a cellulosic substrate, is becoming increasingly recognized as one of the key steps in the enzymatic deconstruction of cellulosic biomass when used as a feedstock for fuels and chemicals production. Although this process ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-5-51

    authors: Gourlay K,Arantes V,Saddler JN

    更新日期:2012-07-24 00:00:00

  • Lavender- and lavandin-distilled straws: an untapped feedstock with great potential for the production of high-added value compounds and fungal enzymes.

    abstract:Background:Lavender (Lavandula angustifolia) and lavandin (a sterile hybrid of L. angustifolia × L. latifolia) essential oils are among those most commonly used in the world for various industrial purposes, including perfumes, pharmaceuticals and cosmetics. The solid residues from aromatic plant distillation such as la...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1218-5

    authors: Lesage-Meessen L,Bou M,Ginies C,Chevret D,Navarro D,Drula E,Bonnin E,Del Río JC,Odinot E,Bisotto A,Berrin JG,Sigoillot JC,Faulds CB,Lomascolo A

    更新日期:2018-08-02 00:00:00

  • Metabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle.

    abstract:Background:S-Adenosylmethionine (SAM) is a critical cofactor involved in many biochemical reactions. However, the low fermentation titer of SAM in methionine-free medium hampers commercial-scale production. The SAM synthesis pathway is specially related to the tricarboxylic acid (TCA) cycle in Bacillus amyloliquefacien...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1554-0

    authors: Ruan L,Li L,Zou D,Jiang C,Wen Z,Chen S,Deng Y,Wei X

    更新日期:2019-09-09 00:00:00

  • Butanol production from laccase-pretreated brewer's spent grain.

    abstract:Background:Beer is the most popular alcoholic beverage worldwide. In the manufacture of beer, various by-products and residues are generated, and the most abundant (85% of total by-products) are spent grains. Thanks to its high (hemi)cellulose content (about 50% w/w dry weight), this secondary raw material is attractiv...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1383-1

    authors: Giacobbe S,Piscitelli A,Raganati F,Lettera V,Sannia G,Marzocchella A,Pezzella C

    更新日期:2019-03-05 00:00:00

  • Evaluation of native microalgae from Tunisia using the pulse-amplitude-modulation measurement of chlorophyll fluorescence and a performance study in semi-continuous mode for biofuel production.

    abstract:Background:Microalgae are attracting much attention as a promising feedstock for renewable energy production, while simultaneously providing environmental benefits. So far, comparison studies for microalgae selection for this purpose were mainly based on data obtained from batch cultures, where the lipid content and th...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1461-4

    authors: Jebali A,Acién FG,Jiménez-Ruiz N,Gómez C,Fernández-Sevilla JM,Mhiri N,Karray F,Sayadi S,Molina-Grima E

    更新日期:2019-05-11 00:00:00

  • Understanding the structural characteristics of water-soluble phenolic compounds from four pretreatments of corn stover and their inhibitory effects on enzymatic hydrolysis and fermentation.

    abstract:Background:For bioethanol production from lignocellulosic biomass, phenolics derived from pretreatment have been generally considered as highly inhibitory towards enzymatic hydrolysis and fermentation. As phenolics are produced from lignin degradation during pretreatment, it is likely that the pretreatment will exert a...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-020-01686-z

    authors: Chen X,Zhai R,Li Y,Yuan X,Liu ZH,Jin M

    更新日期:2020-03-11 00:00:00

  • Direct and up-close views of plant cell walls show a leading role for lignin-modifying enzymes on ensuing xylanases.

    abstract:BACKGROUND:A key barrier that limits the full potential of biological processes to create new, sustainable materials and fuels from plant fibre is limited enzyme accessibility to polysaccharides and lignin that characterize lignocellulose networks. Moreover, the heterogeneity of lignocellulosic substrates means that di...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0176-9

    authors: Jeremic D,Goacher RE,Yan R,Karunakaran C,Master ER

    更新日期:2014-12-31 00:00:00

  • Production of 2,3-butanediol from glucose and cassava hydrolysates by metabolically engineered industrial polyploid Saccharomyces cerevisiae.

    abstract:Background:2,3-Butanediol (2,3-BDO) is a valuable chemical for industrial applications. Bacteria can produce 2,3-BDO with a high productivity, though most of their classification as pathogens makes them undesirable for the industrial-scale production. Though Saccharomyces cerevisiae (GRAS microorganism) was engineered ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1545-1

    authors: Lee YG,Seo JH

    更新日期:2019-08-29 00:00:00

  • In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases.

    abstract:BACKGROUND:P450 fatty acid decarboxylases represented by the unusual CYP152 peroxygenase family member OleTJE have been receiving great attention recently since these P450 enzymes are able to catalyze the simple and direct production of 1-alkenes for potential applications in biofuels and biomaterials. To gain more mec...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0894-x

    authors: Xu H,Ning L,Yang W,Fang B,Wang C,Wang Y,Xu J,Collin S,Laeuffer F,Fourage L,Li S

    更新日期:2017-09-07 00:00:00

  • Characterization of a novel sugar transporter involved in sugarcane bagasse degradation in Trichoderma reesei.

    abstract:Background:Trichoderma reesei is a saprophytic fungus implicated in the degradation of polysaccharides present in the cell wall of plants. T. reesei has been recognized as the most important industrial fungus that secretes and produces cellulase enzymes that are employed in the production of second generation bioethano...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1084-1

    authors: Nogueira KMV,de Paula RG,Antoniêto ACC,Dos Reis TF,Carraro CB,Silva AC,Almeida F,Rechia CGV,Goldman GH,Silva RN

    更新日期:2018-04-02 00:00:00

  • Elucidation of the effect of ionic liquid pretreatment on rice husk via structural analyses.

    abstract:UNLABELLED: BACKGROUND:In the present study, three ionic liquids, namely 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc), and 1-ethyl-3-methylimidazolium diethyl phosphate ([EMIM]DEP), were used to partially dissolve rice husk, after which the cellulose were regenerated...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-5-67

    authors: Ang TN,Ngoh GC,Chua AS,Lee MG

    更新日期:2012-09-07 00:00:00

  • A green-light inducible lytic system for cyanobacterial cells.

    abstract:BACKGROUND:Cyanobacteria are an attractive candidate for the production of biofuel because of their ability to capture carbon dioxide by photosynthesis and grow on non-arable land. However, because huge quantities of water are required for cultivation, strict water management is one of the greatest issues in algae- and...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-56

    authors: Miyake K,Abe K,Ferri S,Nakajima M,Nakamura M,Yoshida W,Kojima K,Ikebukuro K,Sode K

    更新日期:2014-04-09 00:00:00

  • Reduced use of phosphorus and water in sequential dark fermentation and anaerobic digestion of wheat straw and the application of ensiled steam-pretreated lucerne as a macronutrient provider in anaerobic digestion.

    abstract:Background:Current EU directives demand increased use of renewable fuels in the transportation sector but restrict governmental support for production of biofuels produced from crops. The use of intercropped lucerne and wheat may comply with the directives. In the current study, the combination of ensiled lucerne (Medi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1280-z

    authors: Byrne E,Kovacs K,van Niel EWJ,Willquist K,Svensson SE,Kreuger E

    更新日期:2018-10-11 00:00:00

  • Sexual crossing of thermophilic fungus Myceliophthora heterothallica improved enzymatic degradation of sugar beet pulp.

    abstract:BACKGROUND:Enzymatic degradation of plant biomass requires a complex mixture of many different enzymes. Like most fungi, thermophilic Myceliophthora species therefore have a large set of enzymes targeting different linkages in plant polysaccharides. The majority of these enzymes have not been functionally characterized...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0460-y

    authors: Aguilar-Pontes MV,Zhou M,van der Horst S,Theelen B,de Vries RP,van den Brink J

    更新日期:2016-02-20 00:00:00

  • RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa.

    abstract:BACKGROUND:Low- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental glucose fluctuation for their growth and competitiveness. In Neurospora crassa, the dual-affinity glucose transport system consists of a low-affinity glucose transporter GLT-1 and two high-affi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-021-01877-2

    authors: Li J,Liu Q,Li J,Lin L,Li X,Zhang Y,Tian C

    更新日期:2021-01-28 00:00:00

  • Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw.

    abstract:Background:A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solution of renewable routes to chemicals and fuels. Members of the genus Geobacillus are able to grow at...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1540-6

    authors: Bashir Z,Sheng L,Anil A,Lali A,Minton NP,Zhang Y

    更新日期:2019-08-20 00:00:00

  • Transcriptomic response to prolonged ethanol production in the cyanobacterium Synechocystis sp. PCC6803.

    abstract:BACKGROUND:The production of biofuels in photosynthetic microalgae and cyanobacteria is a promising alternative to the generation of fuels from fossil resources. To be economically competitive, producer strains need to be established that synthesize the targeted product at high yield and over a long time. Engineering c...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-21

    authors: Dienst D,Georg J,Abts T,Jakorew L,Kuchmina E,Börner T,Wilde A,Dühring U,Enke H,Hess WR

    更新日期:2014-02-06 00:00:00

  • Deciphering lignocellulose deconstruction by the white rot fungus Irpex lacteus based on genomic and transcriptomic analyses.

    abstract:Background:Irpex lacteus is one of the most potent white rot fungi for biological pretreatment of lignocellulose for second biofuel production. To elucidate the underlying molecular mechanism involved in lignocellulose deconstruction, genomic and transcriptomic analyses were carried out for I. lacteus CD2 grown in subm...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1060-9

    authors: Qin X,Su X,Luo H,Ma R,Yao B,Ma F

    更新日期:2018-03-02 00:00:00

  • Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose.

    abstract:BACKGROUND:β-Glucosidase is an important component of the cellulase enzyme system. It does not only participate in cellulose degradation, it also plays an important role in hydrolyzing cellulose to fermentable glucose by relieving the inhibition of exoglucanase and endoglucanase from cellobiose. Therefore, the glucose-...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-5-31

    authors: Pei J,Pang Q,Zhao L,Fan S,Shi H

    更新日期:2012-07-11 00:00:00