当前位置： SCI文献检索 > Biotechnology for Biofuels期刊下所有文献 > Metabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle.

Metabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle.

Abstract：

Background:S-Adenosylmethionine (SAM) is a critical cofactor involved in many biochemical reactions. However, the low fermentation titer of SAM in methionine-free medium hampers commercial-scale production. The SAM synthesis pathway is specially related to the tricarboxylic acid (TCA) cycle in Bacillus amyloliquefaciens. Therefore, the SAM synthesis pathway was engineered and coupled with the TCA cycle in B. amyloliquefaciens to improve SAM production in methionine-free medium. Results:Four genes were found to significantly affect SAM production, including SAM2 from Saccharomyces cerevisiae, metA and metB from Escherichia coli, and native mccA. These four genes were combined to engineer the SAM pathway, resulting in a 1.42-fold increase in SAM titer using recombinant strain HSAM1. The engineered SAM pathway was subsequently coupled with the TCA cycle through deletion of succinyl-CoA synthetase gene sucC, and the resulted HSAM2 mutant produced a maximum SAM titer of 107.47 mg/L, representing a 0.59-fold increase over HSAM1. Expression of SAM2 in this strain via a recombinant plasmid resulted in strain HSAM3 that produced 648.99 mg/L SAM following semi-continuous flask batch fermentation, a much higher yield than previously reported for methionine-free medium. Conclusions:This study reports an efficient strategy for improving SAM production that can also be applied for generation of SAM cofactors supporting group transfer reactions, which could benefit metabolic engineering, chemical biology and synthetic biology.

journal_name

Biotechnol Biofuels

journal_title

Biotechnology for biofuels

authors

Ruan L,Li L,Zou D,Jiang C,Wen Z,Chen S,Deng Y,Wei X

doi

10.1186/s13068-019-1554-0

subject

Has Abstract

pub_date

2019-09-09 00:00:00

pages

211

issn

1754-6834

pii

1554

journal_volume

12

pub_type

杂志文章

相关文献

Biotechnology for Biofuels文献大全
  • Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar.

    abstract：BACKGROUND:Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The effects of incubation ...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-6-42

    authors： Wang K,Yang H,Wang W,Sun RC

    更新日期：2013-03-21 00:00:00

  • The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973.

    abstract：Background:Cyanobacteria have shown promising potential for the production of various biofuels and chemical feedstocks. Synechococcus elongatus UTEX 2973 is a fast-growing strain with pronounced tolerance to high temperatures and illumination. Hence, this strain appears to be ideal for the development of photosynthetic...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-018-1215-8

    authors： Tan X,Hou S,Song K,Georg J,Klähn S,Lu X,Hess WR

    更新日期：2018-08-04 00:00:00

  • Elucidation of the effect of ionic liquid pretreatment on rice husk via structural analyses.

    abstract：UNLABELLED: BACKGROUND:In the present study, three ionic liquids, namely 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc), and 1-ethyl-3-methylimidazolium diethyl phosphate ([EMIM]DEP), were used to partially dissolve rice husk, after which the cellulose were regenerated...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-5-67

    authors： Ang TN,Ngoh GC,Chua AS,Lee MG

    更新日期：2012-09-07 00:00:00

  • Increased drought tolerance in plants engineered for low lignin and low xylan content.

    abstract：Background:We previously developed several strategies to engineer plants to produce cost-efficient biofuels from plant biomass. Engineered Arabidopsis plants with low xylan and lignin content showed normal growth and improved saccharification efficiency under standard growth conditions. However, it remains to be determ...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-018-1196-7

    authors： Yan J,Aznar A,Chalvin C,Birdseye DS,Baidoo EEK,Eudes A,Shih PM,Loqué D,Zhang A,Scheller HV

    更新日期：2018-07-18 00:00:00

  • Characterization of a novel sugar transporter involved in sugarcane bagasse degradation in Trichoderma reesei.

    abstract：Background:Trichoderma reesei is a saprophytic fungus implicated in the degradation of polysaccharides present in the cell wall of plants. T. reesei has been recognized as the most important industrial fungus that secretes and produces cellulase enzymes that are employed in the production of second generation bioethano...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-018-1084-1

    authors： Nogueira KMV,de Paula RG,Antoniêto ACC,Dos Reis TF,Carraro CB,Silva AC,Almeida F,Rechia CGV,Goldman GH,Silva RN

    更新日期：2018-04-02 00:00:00

  • Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains.

    abstract：BACKGROUND:Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic biomass. The commercial enzyme mixtures produced at industrial scales are not well characterized, and ...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-1-18

    authors： Herpoël-Gimbert I,Margeot A,Dolla A,Jan G,Mollé D,Lignon S,Mathis H,Sigoillot JC,Monot F,Asther M

    更新日期：2008-12-23 00:00:00

  • Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw.

    abstract：Background:A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solution of renewable routes to chemicals and fuels. Members of the genus Geobacillus are able to grow at...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-019-1540-6

    authors： Bashir Z,Sheng L,Anil A,Lali A,Minton NP,Zhang Y

    更新日期：2019-08-20 00:00:00

  • Enzymatic synthesis of l-fucose from l-fuculose using a fucose isomerase from Raoultella sp. and the biochemical and structural analyses of the enzyme.

    abstract：Background:l-Fucose is a rare sugar with potential uses in the pharmaceutical, cosmetic, and food industries. The enzymatic approach using l-fucose isomerase, which interconverts l-fucose and l-fuculose, can be an efficient way of producing l-fucose for industrial applications. Here, we performed biochemical and struct...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-019-1619-0

    authors： Kim IJ,Kim DH,Nam KH,Kim KH

    更新日期：2019-12-05 00:00:00

  • Engineering a Streptomyces coelicolor biosynthesis pathway into Escherichia coli for high yield triglyceride production.

    abstract：BACKGROUND:Microbial lipid production represents a potential alternative feedstock for the biofuel and oleochemical industries. Since Escherichia coli exhibits many genetic, technical, and biotechnological advantages over native oleaginous bacteria, we aimed to construct a metabolically engineered E. coli strain capabl...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-014-0172-0

    authors： Comba S,Sabatini M,Menendez-Bravo S,Arabolaza A,Gramajo H

    更新日期：2014-12-24 00:00:00

  • Evaluation of native microalgae from Tunisia using the pulse-amplitude-modulation measurement of chlorophyll fluorescence and a performance study in semi-continuous mode for biofuel production.

    abstract：Background:Microalgae are attracting much attention as a promising feedstock for renewable energy production, while simultaneously providing environmental benefits. So far, comparison studies for microalgae selection for this purpose were mainly based on data obtained from batch cultures, where the lipid content and th...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-019-1461-4

    authors： Jebali A,Acién FG,Jiménez-Ruiz N,Gómez C,Fernández-Sevilla JM,Mhiri N,Karray F,Sayadi S,Molina-Grima E

    更新日期：2019-05-11 00:00:00

  • Assessment of drought tolerance of 49 switchgrass (Panicum virgatum) genotypes using physiological and morphological parameters.

    abstract：BACKGROUND:Switchgrass (Panicum virgatum L.) is a warm-season C4 grass that is a target lignocellulosic biofuel species. In many regions, drought stress is one of the major limiting factors for switchgrass growth. The objective of this study was to evaluate the drought tolerance of 49 switchgrass genotypes. The relativ...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-015-0342-8

    authors： Liu Y,Zhang X,Tran H,Shan L,Kim J,Childs K,Ervin EH,Frazier T,Zhao B

    更新日期：2015-09-22 00:00:00

  • Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity.

    abstract：Background:Functional sugar alcohols have been widely used in the food, medicine, and pharmaceutical industries for their unique properties. Among these, erythritol is a zero calories sweetener produced by the yeast Yarrowia lipolytica. However, in wild-type strains, erythritol is produced with low productivity and yie...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-020-01815-8

    authors： Wang N,Chi P,Zou Y,Xu Y,Xu S,Bilal M,Fickers P,Cheng H

    更新日期：2020-10-20 00:00:00

  • De novo transcriptome sequencing and analysis revealed the molecular basis of rapid fat accumulation by black soldier fly (Hermetia illucens, L.) for development of insectival biodiesel.

    abstract：Background:Black soldier fly (BSF, Hermetia illucens L.) can efficiently degrade organic wastes and transform into a high fat containing insect biomass that could be used as feedstock for biodiesel production. Meanwhile, the molecular regulatory basis of fat accumulation by BSF is still unclear; it is necessary to iden...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-019-1531-7

    authors： Zhu Z,Rehman KU,Yu Y,Liu X,Wang H,Tomberlin JK,Sze SH,Cai M,Zhang J,Yu Z,Zheng J,Zheng L

    更新日期：2019-08-09 00:00:00

  • Techno-economic evaluation of stillage treatment with anaerobic digestion in a softwood-to-ethanol process.

    abstract：BACKGROUND:Replacing the energy-intensive evaporation of stillage by anaerobic digestion is one way of decreasing the energy demand of the lignocellulosic biomass to the ethanol process. The biogas can be upgraded and sold as transportation fuel, injected directly into the gas grid or be incinerated on-site for combine...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-3-21

    authors： Barta Z,Reczey K,Zacchi G

    更新日期：2010-09-15 00:00:00

  • Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution.

    abstract：Background:Methane, a main component of natural gas and biogas, has gained much attention as an abundant and low-cost carbon source. Methanotrophs, which can use methane as a sole carbon and energy source, are promising hosts to produce value-added chemicals from methane, but their metabolic engineering is still challe...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-019-1574-9

    authors： Lee JK,Kim S,Kim W,Kim S,Cha S,Moon H,Hur DH,Kim SY,Na JG,Lee JW,Lee EY,Hahn JS

    更新日期：2019-09-30 00:00:00

  • Visualizing cellulase adsorption and quantitatively determining cellulose accessibility with an updated fungal cellulose-binding module-based fluorescent probe protein.

    abstract：Background:Cellulose accessibility to cellulases (CAC) is a direct factor determining the enzymatic digestibility of lignocellulosic cellulose. Improving CAC by pretreatment is a prerequisite step for the efficient release of fermentable sugars from biomass cell wall. However, conventional methods to study the porosime...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-018-1105-0

    authors： Li T,Liu N,Ou X,Zhao X,Qi F,Huang J,Liu D

    更新日期：2018-04-09 00:00:00

  • Peptide-mediated microalgae harvesting method for efficient biofuel production.

    abstract：BACKGROUND:Production of biofuels from microalgae has been recognized to be a promising route for a sustainable energy supply. However, the microalgae harvesting process is a bottleneck for industrialization because it is energy intensive. Thus, by displaying interactive protein factors on the cell wall, oleaginous mic...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-015-0406-9

    authors： Maeda Y,Tateishi T,Niwa Y,Muto M,Yoshino T,Kisailus D,Tanaka T

    更新日期：2016-01-13 00:00:00

  • Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression.

    abstract：BACKGROUND:n-Butanol can serve as an excellent gasoline substitute. Naturally, it is produced by some Clostridia species which, however, exhibit only limited suitability for industrial n-butanol production. The yeast Saccharomyces cerevisiae would be an ideal host due to its high robustness in fermentation processes. N...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-016-0673-0

    authors： Schadeweg V,Boles E

    更新日期：2016-11-25 00:00:00

  • Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products.

    abstract：:Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorization of lignin to chemicals and fuels. However, due to the structural heterogeneity of lignin, depolymerization ...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章,评审

    doi：10.1186/s13068-019-1376-0

    authors： Xu Z,Lei P,Zhai R,Wen Z,Jin M

    更新日期：2019-02-15 00:00:00

  • Evaluation of ammonia fibre expansion (AFEX) pretreatment for enzymatic hydrolysis of switchgrass harvested in different seasons and locations.

    abstract：BACKGROUND:When producing biofuels from dedicated feedstock, agronomic factors such as harvest time and location can impact the downstream production. Thus, this paper studies the effectiveness of ammonia fibre expansion (AFEX) pretreatment on two harvest times (July and October) and ecotypes/locations (Cave-in-Rock (C...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-3-1

    authors： Bals B,Rogers C,Jin M,Balan V,Dale B

    更新日期：2010-01-04 00:00:00

  • Integrated analysis of hydrothermal flow through pretreatment.

    abstract：UNLABELLED: BACKGROUND:The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two conversion systems (simultaneous saccharification and fermentation using yeast and fungal ...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-5-49

    authors： Archambault-Leger V,Shao X,Lynd LR

    更新日期：2012-07-19 00:00:00

  • A novel transcription factor specifically regulates GH11 xylanase genes in Trichoderma reesei.

    abstract：BACKGROUND:The filamentous fungus Trichoderma reesei is widely utilized in industry for cellulase production, but its xylanase activity must be improved to enhance the accessibility of lignocellulose to cellulases. Several transcription factors play important roles in this progress; however, nearly all the reported tra...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-017-0878-x

    authors： Liu R,Chen L,Jiang Y,Zou G,Zhou Z

    更新日期：2017-08-03 00:00:00

  • Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae.

    abstract：BACKGROUND:Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasingly important as a sustainable replacement for traditional petroleum-based production processes depe...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-021-01878-1

    authors： Su HY,Li HY,Xie CY,Fei Q,Cheng KK

    更新日期：2021-01-19 00:00:00

  • Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum.

    abstract：UNLABELLED: BACKGROUND:Industrial production of biofuels and other products by cellulolytic microorganisms is of interest but hindered by the nascent state of genetic tools. Although a genetic system for Clostridium thermocellum DSM1313 has recently been developed, available methods achieve relatively low efficiency a...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-5-30

    authors： Guss AM,Olson DG,Caiazza NC,Lynd LR

    更新日期：2012-05-06 00:00:00

  • SSF of steam-pretreated wheat straw with the addition of saccharified or fermented wheat meal in integrated bioethanol production.

    abstract：BACKGROUND:Integration of second-generation (2G) bioethanol production with existing first-generation (1G) production may facilitate commercial production of ethanol from cellulosic material. Since 2G hydrolysates have a low sugar concentration and 1G streams often have to be diluted prior to fermentation, mixing of st...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-6-169

    authors： Erdei B,Hancz D,Galbe M,Zacchi G

    更新日期：2013-11-29 00:00:00

  • Improving carbohydrate and starch accumulation in Chlorella sp. AE10 by a novel two-stage process with cell dilution.

    abstract：BACKGROUND:Microalgae are highly efficient cellular factories that capture CO2 and are also alternative feedstock for biofuel production. Carbohydrates, proteins, and lipids are major biochemical components in microalgae. Carbohydrates or starch in microalgae are possible substrates in yeast fermentation for biofuel pr...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-017-0753-9

    authors： Cheng D,Li D,Yuan Y,Zhou L,Li X,Wu T,Wang L,Zhao Q,Wei W,Sun Y

    更新日期：2017-03-24 00:00:00

  • Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp. strain PCC 7002 from an oceanic environment.

    abstract：BACKGROUND:Oxygenic photosynthetic microorganisms such as cyanobacteria and microalgae have attracted attention as an alternative carbon source for the next generation of biofuels. Glycogen abundantly accumulated in cyanobacteria is a promising feedstock which can be converted to ethanol through saccharification and fe...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-7-88

    authors： Aikawa S,Nishida A,Ho SH,Chang JS,Hasunuma T,Kondo A

    更新日期：2014-06-11 00:00:00

  • Selecting β-glucosidases to support cellulases in cellulose saccharification.

    abstract：BACKGROUND:Enzyme end-product inhibition is a major challenge in the hydrolysis of lignocellulose at a high dry matter consistency. β-glucosidases (BGs) hydrolyze cellobiose into two molecules of glucose, thereby relieving the product inhibition of cellobiohydrolases (CBHs). However, BG inhibition by glucose will event...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/1754-6834-6-105

    authors： Teugjas H,Väljamäe P

    更新日期：2013-07-24 00:00:00

  • Proteomic and metabolomic analysis of the cellular biomarkers related to inhibitors tolerance in Zymomonas mobilis ZM4.

    abstract：Background:Toxic compounds present in both the hydrolysate and pyrolysate of lignocellulosic biomass severely hinder the further conversion of lignocellulose-derived fermentable sugars into useful chemicals by common biocatalysts like Zymomonas mobilis, which has remarkable advantages over yeast. Although the extra det...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-018-1287-5

    authors： Chang D,Yu Z,Ul Islam Z,French WT,Zhang Y,Zhang H

    更新日期：2018-10-16 00:00:00

  • Meta-proteomic analysis of protein expression distinctive to electricity-generating biofilm communities in air-cathode microbial fuel cells.

    abstract：Background:Bioelectrochemical systems (BESs) harness electrons from microbial respiration to generate power or chemical products from a variety of organic feedstocks, including lignocellulosic biomass, fermentation byproducts, and wastewater sludge. In some BESs, such as microbial fuel cells (MFCs), bacteria living in ...

    journal_title：Biotechnology for biofuels

    pub_type： 杂志文章

    doi：10.1186/s13068-018-1111-2

    authors： Chignell JF,De Long SK,Reardon KF

    更新日期：2018-04-23 00:00:00