What cell wall components are the best indicators for Miscanthus digestibility and conversion to ethanol following variable pretreatments?

abstract：BACKGROUND:As a green alternative for the production of transportation fuels, the enzymatic hydrolysis of lignocellulose and subsequent fermentation to ethanol are being intensively researched. To be economically feasible, the hydrolysis of lignocellulose must be conducted at a high concentration of solids, which resul...

journal_title：Biotechnology for biofuels

authors： Teugjas H,Väljamäe P

更新日期：2013-07-24 00:00:00

abstract：BACKGROUND:The most advanced strains of xylose-fermenting Saccharomyces cerevisiae still utilize xylose far less efficiently than glucose, despite the extensive metabolic and evolutionary engineering applied in their development. Systematic comparison of strains across literature is difficult due to widely varying cond...

journal_title：Biotechnology for biofuels

authors： Novy V,Wang R,Westman JO,Franzén CJ,Nidetzky B

更新日期：2017-09-04 00:00:00

abstract：Background:Inefficient utilization of glycerol by Clostridium beijerinckii (Cb) is a major impediment to adopting glycerol metabolism as a strategy for increasing NAD(P)H regeneration, which would in turn, alleviate the toxicity of lignocellulose-derived microbial inhibitory compounds (LDMICs, e.g., furfural), and impr...

journal_title：Biotechnology for biofuels

authors： Agu CV,Ujor V,Ezeji TC

更新日期：2019-03-09 00:00:00

abstract：BACKGROUND:Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic biomass. The commercial enzyme mixtures produced at industrial scales are not well characterized, and ...

journal_title：Biotechnology for biofuels

authors： Herpoël-Gimbert I,Margeot A,Dolla A,Jan G,Mollé D,Lignon S,Mathis H,Sigoillot JC,Monot F,Asther M

更新日期：2008-12-23 00:00:00

abstract：: The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its value on market was reduced and it is becoming a "waste-stream" instead of a valuable "coproduct". The developmen...

journal_title：Biotechnology for biofuels

authors： Almeida JR,Fávaro LC,Quirino BF

更新日期：2012-07-18 00:00:00

abstract：BACKGROUND:Low- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental glucose fluctuation for their growth and competitiveness. In Neurospora crassa, the dual-affinity glucose transport system consists of a low-affinity glucose transporter GLT-1 and two high-affi...

journal_title：Biotechnology for biofuels

authors： Li J,Liu Q,Li J,Lin L,Li X,Zhang Y,Tian C

更新日期：2021-01-28 00:00:00

abstract：BACKGROUND:Thermoanaerobacter saccharolyticum is a thermophilic microorganism that has been engineered to produce ethanol at high titer (30-70 g/L) and greater than 90 % theoretical yield. However, few genes involved in pyruvate to ethanol production pathway have been unambiguously identified. In T. saccharolyticum, th...

journal_title：Biotechnology for biofuels

authors： Zhou J,Olson DG,Lanahan AA,Tian L,Murphy SJ,Lo J,Lynd LR

更新日期：2015-09-15 00:00:00

abstract：BACKGROUND:Coordination of synthesis and assembly of the polymeric components of cell walls is essential for plant growth and development. Given the degree of co-mingling and cross-linking among cell wall components, cellulose organization must be dependent on the organization of other polymers such as lignin. Here we ...

journal_title：Biotechnology for biofuels

authors： Liu J,Kim JI,Cusumano JC,Chapple C,Venugopalan N,Fischetti RF,Makowski L

更新日期：2016-06-17 00:00:00

abstract：BACKGROUND:The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (GH10) from the thermophilic ...

journal_title：Biotechnology for biofuels

authors： Ribeiro LF,De Lucas RC,Vitcosque GL,Ribeiro LF,Ward RJ,Rubio MV,Damásio AR,Squina FM,Gregory RC,Walton PH,Jorge JA,Prade RA,Buckeridge MS,Polizeli Mde L

更新日期：2014-07-29 00:00:00

abstract：BACKGROUND:There are many different types of pretreatment carried out to prepare cellulosic substrates for fermentation. In this study, one- and two-stage hydrothermal pretreatment were carried out to determine their effects on subsequent fermentations. The two substrates were found to behave differently during ferment...

journal_title：Biotechnology for biofuels

authors： Guilliams A,Pattathil S,Willies D,Richards M,Pu Y,Kandemkavil S,Wiswall E

更新日期：2016-02-03 00:00:00

abstract：BACKGROUND:In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofuel from biomass is the enzymatic saccharification. Many of the commercial cellulase enzyme cocktails...

journal_title：Biotechnology for biofuels

authors： Chan CS,Sin LL,Chan KG,Shamsir MS,Manan FA,Sani RK,Goh KM

更新日期：2016-08-22 00:00:00

abstract：:Biorefineries, designed for the production of lignocellulose-based chemicals and fuels, are receiving increasing attention from the public, governments, and industries. A major obstacle for biorefineries to advance to commercial scale is the high cost of the enzymes required to derive the fermentable sugars from the f...

journal_title：Biotechnology for biofuels

authors： Novy V,Nielsen F,Seiboth B,Nidetzky B

更新日期：2019-10-08 00:00:00

abstract：BACKGROUND:Hydrolysates of plant biomass used for the production of lignocellulosic biofuels typically contain sugar mixtures consisting mainly of D-glucose and D-xylose, and minor amounts of L-arabinose. The yeast Saccharomyces cerevisiae is the preferred microorganism for the fermentative production of ethanol but is...

journal_title：Biotechnology for biofuels

authors： Subtil T,Boles E

更新日期：2011-10-12 00:00:00

abstract：Background:n-Butyraldehyde is a high-production volume chemical produced exclusively from hydroformylation of propylene. It is a versatile chemical used in the synthesis of diverse C4-C8 alcohols, carboxylic acids, esters, and amines. Its high demand and broad applications make it an ideal chemical to be produced from ...

journal_title：Biotechnology for biofuels

authors： Ku JT,Simanjuntak W,Lan EI

更新日期：2017-12-04 00:00:00

abstract：BACKGROUND:Investigations on a few eukaryotic model organisms showed that many genes are non-randomly distributed on chromosomes. In addition, chromosome ends frequently possess genes that are important for the fitness of the organisms. Trichoderma reesei is an industrial producer of enzymes for food, feed and biorefin...

journal_title：Biotechnology for biofuels

authors： Druzhinina IS,Kopchinskiy AG,Kubicek EM,Kubicek CP

更新日期：2016-03-29 00:00:00

abstract：Background:Cyanobacteria are ideal model organisms to exploit photosynthetically derived electrons or fixed carbon for the biotechnological synthesis of high value compounds and energy carriers. Much effort is spent on the rational design of heterologous pathways to produce value-added chemicals. Much less focus is dra...

journal_title：Biotechnology for biofuels

authors： Grund M,Jakob T,Wilhelm C,Bühler B,Schmid A

更新日期：2019-02-27 00:00:00

abstract：BACKGROUND:Ionic liquid (IL) pretreatment is receiving significant attention as a potential process that enables fractionation of lignocellulosic biomass and produces high yields of fermentable sugars suitable for the production of renewable fuels. However, successful optimization and scale up of IL pretreatment involv...

journal_title：Biotechnology for biofuels

authors： Li C,Tanjore D,He W,Wong J,Gardner JL,Sale KL,Simmons BA,Singh S

更新日期：2013-10-25 00:00:00

abstract：BACKGROUND:The production of value-added chemicals alongside biofuels from lignocellulosic hydrolysates is critical for developing economically viable biorefineries. Here, the production of propionic acid (PA), a potential building block for C3-based chemicals, from corn stover hydrolysate is investigated using the nat...

journal_title：Biotechnology for biofuels

authors： Wang X,Salvachúa D,Sànchez I Nogué V,Michener WE,Bratis AD,Dorgan JR,Beckham GT

更新日期：2017-08-17 00:00:00

abstract：BACKGROUND:Lignocellulosic materials are abundant and among the most important potential sources for bioethanol production. Although the pretreatment of lignocellulose is necessary for efficient saccharification and fermentation, numerous by-products, including furan derivatives, weak acids, and phenolic compounds, are...

journal_title：Biotechnology for biofuels

authors： Endo A,Nakamura T,Ando A,Tokuyasu K,Shima J

更新日期：2008-04-15 00:00:00

abstract：: The production of cellulosic ethanol from biomass is considered a promising alternative to reliance on diminishing supplies of fossil fuels, providing a sustainable option for fuels production in an environmentally compatible manner. The conversion of lignocellulosic biomass to biofuels through a biological route usu...

journal_title：Biotechnology for biofuels

authors： Pu Y,Hu F,Huang F,Davison BH,Ragauskas AJ

更新日期：2013-01-28 00:00:00

abstract：BACKGROUND:Although lignin peroxidase is claimed as a key enzyme in enzyme-catalyzed lignin degradation, in vitro enzymatic degradation of lignin was not easily observed in lab-scale experiments. It implies that other factors may hinder the enzymatic degradation of lignin. Irreversible interaction between phenolic comp...

journal_title：Biotechnology for biofuels

authors： Pham LT,Kim SJ,Kim YH

更新日期：2016-11-15 00:00:00

abstract：Background:Methane, a main component of natural gas and biogas, has gained much attention as an abundant and low-cost carbon source. Methanotrophs, which can use methane as a sole carbon and energy source, are promising hosts to produce value-added chemicals from methane, but their metabolic engineering is still challe...

journal_title：Biotechnology for biofuels

authors： Lee JK,Kim S,Kim W,Kim S,Cha S,Moon H,Hur DH,Kim SY,Na JG,Lee JW,Lee EY,Hahn JS

更新日期：2019-09-30 00:00:00

abstract：Background:Saccharification is the most crucial and cost-intensive process in second generation biofuel production. The deficiency of β-glucosidase in commercial enzyme leads to incomplete biomass hydrolysis. The decomposition of biomass at high temperature environments leads us to isolate thermotolerant microbes with ...

journal_title：Biotechnology for biofuels

authors： Tiwari R,Singh PK,Singh S,Nain PKS,Nain L,Shukla P

更新日期：2017-10-30 00:00:00

abstract：Background:Clostridium thermocellum is a promising microorganism for conversion of cellulosic biomass to biofuel, without added enzymes; however, the low ethanol titer produced by strains developed thus far is an obstacle to industrial application. Results:Here, we analyzed changes in the relative concentration of int...

journal_title：Biotechnology for biofuels

authors： Tian L,Perot SJ,Stevenson D,Jacobson T,Lanahan AA,Amador-Noguez D,Olson DG,Lynd LR

更新日期：2017-11-30 00:00:00

abstract：BACKGROUND:Saccharomyces cerevisiae, a key organism used for the manufacture of renewable fuels and chemicals, has been engineered to utilize non-native sugars derived from plant cell walls, such as cellobiose and xylose. However, the rates and efficiencies of these non-native sugar fermentations pale in comparison wit...

journal_title：Biotechnology for biofuels

authors： Lin Y,Chomvong K,Acosta-Sampson L,Estrela R,Galazka JM,Kim SR,Jin YS,Cate JH

更新日期：2014-08-27 00:00:00

abstract：Background:There is an urgent requirement for second-generation bio-based industries for economical yet efficient enzymatic cocktail to convert diverse cellulosic biomass into fermentable sugars. In our previous study, secretome of Penicillium funiculosum NCIM1228 showed high commercial potential by exhibiting high bio...

journal_title：Biotechnology for biofuels

authors： Randhawa A,Ogunyewo OA,Eqbal D,Gupta M,Yazdani SS

更新日期：2018-01-25 00:00:00

abstract：BACKGROUND:Production of valuable metabolites by Yarrowia lipolytica using renewable raw materials is of major interest for sustainable food and energy. Galactose is a monosaccharide found in galactomannans, hemicelluloses, gums, and pectins. RESULTS:Yarrowia lipolytica was found to express all the Leloir pathway gene...

journal_title：Biotechnology for biofuels

authors： Lazar Z,Gamboa-Meléndez H,Le Coq AM,Neuvéglise C,Nicaud JM

更新日期：2015-11-25 00:00:00

abstract：BACKGROUND:Glycoside hydrolases are important for various industrial and scientific applications. Determination of their temperature as well as pH optima and range is crucial to evaluate whether an enzyme is suitable for application in a biotechnological process. These basic characteristics of enzymes are generally det...

journal_title：Biotechnology for biofuels

authors： Herlet J,Kornberger P,Roessler B,Glanz J,Schwarz WH,Liebl W,Zverlov VV

更新日期：2017-10-11 00:00:00

abstract：BACKGROUND:The primary components of lignocellulosic biomass such as sorghum bagasse are cellulose, hemicellulose, and lignin. Each component can be utilized as a sustainable resource for producing biofuels and bio-based products. However, due to their complicated structures, fractionation of lignocellulosic biomass co...

journal_title：Biotechnology for biofuels

authors： Teramura H,Sasaki K,Oshima T,Matsuda F,Okamoto M,Shirai T,Kawaguchi H,Ogino C,Hirano K,Sazuka T,Kitano H,Kikuchi J,Kondo A

更新日期：2016-02-02 00:00:00

abstract：BACKGROUND:D-2,3-butanediol has many industrial applications such as chiral reagents, solvents, anti-freeze agents, and low freezing point fuels. Traditional D-2,3-butanediol producing microorganisms, such as Klebsiella pneumonia and K. xoytoca, are pathogenic and not capable of producing D-2,3-butanediol at high optic...

journal_title：Biotechnology for biofuels

authors： Qi G,Kang Y,Li L,Xiao A,Zhang S,Wen Z,Xu D,Chen S

更新日期：2014-01-29 00:00:00