Renewable synthesis of n-butyraldehyde from glucose by engineered Escherichia coli.

Abstract:

Background:n-Butyraldehyde is a high-production volume chemical produced exclusively from hydroformylation of propylene. It is a versatile chemical used in the synthesis of diverse C4-C8 alcohols, carboxylic acids, esters, and amines. Its high demand and broad applications make it an ideal chemical to be produced from biomass. Results:An Escherichia coli strain was engineered to produce n-butyraldehyde directly from glucose by expressing a modified Clostridium CoA-dependent n-butanol production pathway with mono-functional Coenzyme A-acylating aldehyde dehydrogenase (Aldh) instead of the natural bifunctional aldehyde/alcohol dehydrogenase. Aldh from Clostridium beijerinckii outperformed the other tested homologues. However, the presence of native alcohol dehydrogenase led to spontaneous conversion of n-butyraldehyde to n-butanol. This problem was addressed by knocking out native E. coli alcohol dehydrogenases, significantly improving the butyraldehyde-to-butanol ratio. This ratio was further increased reducing media complexity from Terrific broth to M9 media containing 2% yeast extract. To increase production titer, in situ liquid-liquid extraction using dodecane and oleyl alcohol was investigated. Results showed oleyl alcohol as a better extractant, increasing the titer of n-butyraldehyde produced to 630 mg/L. Conclusion:This study demonstrated n-butyraldehyde production from glucose. Through sequential strain and condition optimizations, butyraldehyde-to-butanol ratio was improved significantly compared to the parent strain. Results from this work may serve as a basis for further development of renewable n-butyraldehyde production.

journal_name

Biotechnol Biofuels

authors

Ku JT,Simanjuntak W,Lan EI

doi

10.1186/s13068-017-0978-7

subject

Has Abstract

pub_date

2017-12-04 00:00:00

pages

291

issn

1754-6834

pii

978

journal_volume

10

pub_type

杂志文章
  • Assessment of drought tolerance of 49 switchgrass (Panicum virgatum) genotypes using physiological and morphological parameters.

    abstract:BACKGROUND:Switchgrass (Panicum virgatum L.) is a warm-season C4 grass that is a target lignocellulosic biofuel species. In many regions, drought stress is one of the major limiting factors for switchgrass growth. The objective of this study was to evaluate the drought tolerance of 49 switchgrass genotypes. The relativ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0342-8

    authors: Liu Y,Zhang X,Tran H,Shan L,Kim J,Childs K,Ervin EH,Frazier T,Zhao B

    更新日期:2015-09-22 00:00:00

  • Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure.

    abstract:BACKGROUND:Higher ratios of syringyl-to-guaiacyl (S/G) lignin components of Populus were shown to improve sugar release by enzymatic hydrolysis using commercial blends. Cellulolytic microbes are often robust biomass hydrolyzers and may offer cost advantages; however, it is unknown whether their activity can also be sig...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-016-0445-x

    authors: Dumitrache A,Akinosho H,Rodriguez M Jr,Meng X,Yoo CG,Natzke J,Engle NL,Sykes RW,Tschaplinski TJ,Muchero W,Ragauskas AJ,Davison BH,Brown SD

    更新日期:2016-02-04 00:00:00

  • Lignolytic-consortium omics analyses reveal novel genomes and pathways involved in lignin modification and valorization.

    abstract:Background:Lignin is a heterogeneous polymer representing a renewable source of aromatic and phenolic bio-derived products for the chemical industry. However, the inherent structural complexity and recalcitrance of lignin makes its conversion into valuable chemicals a challenge. Natural microbial communities produce bi...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1073-4

    authors: Moraes EC,Alvarez TM,Persinoti GF,Tomazetto G,Brenelli LB,Paixão DAA,Ematsu GC,Aricetti JA,Caldana C,Dixon N,Bugg TDH,Squina FM

    更新日期:2018-03-22 00:00:00

  • Bioelectrochemical production of hydrogen in an innovative pressure-retarded osmosis/microbial electrolysis cell system: experiments and modeling.

    abstract:BACKGROUND:While microbial electrolysis cells (MECs) can simultaneously produce bioelectrochemical hydrogen and treat wastewater, they consume considerable energy to overcome the unfavorable thermodynamics, which is not sustainable and economically feasible in practical applications. This study presents a proof-of-conc...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0305-0

    authors: Yuan H,Lu Y,Abu-Reesh IM,He Z

    更新日期:2015-08-14 00:00:00

  • Increased drought tolerance in plants engineered for low lignin and low xylan content.

    abstract:Background:We previously developed several strategies to engineer plants to produce cost-efficient biofuels from plant biomass. Engineered Arabidopsis plants with low xylan and lignin content showed normal growth and improved saccharification efficiency under standard growth conditions. However, it remains to be determ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1196-7

    authors: Yan J,Aznar A,Chalvin C,Birdseye DS,Baidoo EEK,Eudes A,Shih PM,Loqué D,Zhang A,Scheller HV

    更新日期:2018-07-18 00:00:00

  • Stochastic techno-economic analysis of alcohol-to-jet fuel production.

    abstract:BACKGROUND:Alcohol-to-jet (ATJ) is one of the technical feasible biofuel technologies. It produces jet fuel from sugary, starchy, and lignocellulosic biomass, such as sugarcane, corn grain, and switchgrass, via fermentation of sugars to ethanol or other alcohols. This study assesses the ATJ biofuel production pathway f...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0702-7

    authors: Yao G,Staples MD,Malina R,Tyner WE

    更新日期:2017-01-19 00:00:00

  • From grass to gas: microbiome dynamics of grass biomass acidification under mesophilic and thermophilic temperatures.

    abstract:BACKGROUND:Separating acidification and methanogenic steps in anaerobic digestion processes can help to optimize the process and contribute to producing valuable sub-products such as methane, hydrogen and organic acids. However, the full potential of this technology has not been fully explored yet. To assess the underl...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0859-0

    authors: Abendroth C,Simeonov C,Peretó J,Antúnez O,Gavidia R,Luschnig O,Porcar M

    更新日期:2017-07-03 00:00:00

  • Genomic comparison of Clostridium species with the potential of utilizing red algal biomass for biobutanol production.

    abstract:Background:Sustainable biofuels, which are widely considered as an attractive alternative to fossil fuels, can be generated by utilizing various biomass from the environment. Marine biomass, such as red algal biomass, is regarded as one potential renewable substrate source for biofuels conversion due to its abundance o...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1044-9

    authors: Sun C,Zhang S,Xin F,Shanmugam S,Wu YR

    更新日期:2018-02-15 00:00:00

  • Enzymatic synthesis of l-fucose from l-fuculose using a fucose isomerase from Raoultella sp. and the biochemical and structural analyses of the enzyme.

    abstract:Background:l-Fucose is a rare sugar with potential uses in the pharmaceutical, cosmetic, and food industries. The enzymatic approach using l-fucose isomerase, which interconverts l-fucose and l-fuculose, can be an efficient way of producing l-fucose for industrial applications. Here, we performed biochemical and struct...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1619-0

    authors: Kim IJ,Kim DH,Nam KH,Kim KH

    更新日期:2019-12-05 00:00:00

  • Seed-specific suppression of ADP-glucose pyrophosphorylase in Camelina sativa increases seed size and weight.

    abstract:Background:Camelina (Camelina sativa L.) is a promising oilseed crop that may provide sustainable feedstock for biofuel production. One of the major drawbacks of Camelina is its smaller seeds compared to other major oil crops such as canola, which limit oil yield and may also pose challenges in successful seedling esta...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1334-2

    authors: Na G,Aryal N,Fatihi A,Kang J,Lu C

    更新日期:2018-12-18 00:00:00

  • How does technology pathway choice influence economic viability and environmental impacts of lignocellulosic biorefineries?

    abstract:Background:The need for liquid fuels in the transportation sector is increasing, and it is essential to develop industrially sustainable processes that simultaneously address the tri-fold sustainability metrics of technological feasibility, economic viability, and environmental impacts. Biorefineries based on lignocell...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0959-x

    authors: Rajendran K,Murthy GS

    更新日期:2017-11-14 00:00:00

  • Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance.

    abstract:Background:Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by converting it to xylulose. For this, the type II xylose isomerase ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1643-0

    authors: Lee M,Rozeboom HJ,Keuning E,de Waal P,Janssen DB

    更新日期:2020-01-11 00:00:00

  • SSF of steam-pretreated wheat straw with the addition of saccharified or fermented wheat meal in integrated bioethanol production.

    abstract:BACKGROUND:Integration of second-generation (2G) bioethanol production with existing first-generation (1G) production may facilitate commercial production of ethanol from cellulosic material. Since 2G hydrolysates have a low sugar concentration and 1G streams often have to be diluted prior to fermentation, mixing of st...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-169

    authors: Erdei B,Hancz D,Galbe M,Zacchi G

    更新日期:2013-11-29 00:00:00

  • Enhancing oil production in Arabidopsis through expression of a ketoacyl-ACP synthase domain of the PUFA synthase from Thraustochytrium.

    abstract:Background:Plant seed oil is an important bioresource for human food and animal feed, as well as industrial bioproducts. Therefore, increasing oil content in seeds has been one of the primary targets in the breeding programs of oilseed crops. Thraustochytrium is a marine protist that can produce a high level of very lo...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1514-8

    authors: Xie X,Meesapyodsuk D,Qiu X

    更新日期:2019-06-29 00:00:00

  • Visualizing cellulase adsorption and quantitatively determining cellulose accessibility with an updated fungal cellulose-binding module-based fluorescent probe protein.

    abstract:Background:Cellulose accessibility to cellulases (CAC) is a direct factor determining the enzymatic digestibility of lignocellulosic cellulose. Improving CAC by pretreatment is a prerequisite step for the efficient release of fermentable sugars from biomass cell wall. However, conventional methods to study the porosime...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1105-0

    authors: Li T,Liu N,Ou X,Zhao X,Qi F,Huang J,Liu D

    更新日期:2018-04-09 00:00:00

  • Thermophilic fermentation of acetoin and 2,3-butanediol by a novel Geobacillus strain.

    abstract:UNLABELLED: BACKGROUND:Acetoin and 2,3-butanediol are two important biorefinery platform chemicals. They are currently fermented below 40°C using mesophilic strains, but the processes often suffer from bacterial contamination. RESULTS:This work reports the isolation and identification of a novel aerobic Geobacillus s...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-5-88

    authors: Xiao Z,Wang X,Huang Y,Huo F,Zhu X,Xi L,Lu JR

    更新日期:2012-12-06 00:00:00

  • Metabolic engineering of Escherichia coli for production of n-butanol from crude glycerol.

    abstract:BACKGROUND:Crude glycerol in the waste stream of the biodiesel production process is an abundant and renewable resource. However, the glycerol-based industry is usually afflicted by the cost for refinement of crude glycerol. This issue can be addressed by developing a microbial process to convert crude glycerol to valu...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-017-0857-2

    authors: Saini M,Wang ZW,Chiang CJ,Chao YP

    更新日期:2017-07-04 00:00:00

  • Potential of Zymomonas mobilis as an electricity producer in ethanol production.

    abstract:Background:Microbial fuel cell (MFC) convokes microorganism to convert biomass into electricity. However, most well-known electrogenic strains cannot directly use glucose to produce valuable products. Zymomonas mobilis, a promising bacterium for ethanol production, owns special Entner-Doudoroff pathway with less ATP an...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-020-01672-5

    authors: Geng BY,Cao LY,Li F,Song H,Liu CG,Zhao XQ,Bai FW

    更新日期:2020-03-05 00:00:00

  • Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions.

    abstract:BACKGROUND:Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. RESULTS:In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculu...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0286-z

    authors: Rakicka M,Lazar Z,Dulermo T,Fickers P,Nicaud JM

    更新日期:2015-07-25 00:00:00

  • Direct and up-close views of plant cell walls show a leading role for lignin-modifying enzymes on ensuing xylanases.

    abstract:BACKGROUND:A key barrier that limits the full potential of biological processes to create new, sustainable materials and fuels from plant fibre is limited enzyme accessibility to polysaccharides and lignin that characterize lignocellulose networks. Moreover, the heterogeneity of lignocellulosic substrates means that di...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0176-9

    authors: Jeremic D,Goacher RE,Yan R,Karunakaran C,Master ER

    更新日期:2014-12-31 00:00:00

  • Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories.

    abstract:BACKGROUND:Photosynthetic production of chemicals and fuels by recycling CO2 in cyanobacteria is a promising solution facing energy shortage and resource declination. Ethanol is an attractive and demonstrative biofuel product, and ethanol synthesis in cyanobacteria has been achieved by assembling of a pathway consistin...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0367-z

    authors: Luan G,Qi Y,Wang M,Li Z,Duan Y,Tan X,Lu X

    更新日期:2015-11-21 00:00:00

  • Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered Synechocystis sp. PCC 6803.

    abstract:Background:Cyanobacteria are potential sources for third generation biofuels. Their capacity for biofuel production has been widely improved using metabolically engineered strains. In this study, we employed metabolic engineering design with target genes involved in selected processes including the fatty acid synthesis...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-018-1349-8

    authors: Eungrasamee K,Miao R,Incharoensakdi A,Lindblad P,Jantaro S

    更新日期:2019-01-04 00:00:00

  • Production of 2,3-butanediol from glucose and cassava hydrolysates by metabolically engineered industrial polyploid Saccharomyces cerevisiae.

    abstract:Background:2,3-Butanediol (2,3-BDO) is a valuable chemical for industrial applications. Bacteria can produce 2,3-BDO with a high productivity, though most of their classification as pathogens makes them undesirable for the industrial-scale production. Though Saccharomyces cerevisiae (GRAS microorganism) was engineered ...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-019-1545-1

    authors: Lee YG,Seo JH

    更新日期:2019-08-29 00:00:00

  • Electrolytic extraction drives volatile fatty acid chain elongation through lactic acid and replaces chemical pH control in thin stillage fermentation.

    abstract:BACKGROUND:Volatile fatty acids (VFA) are building blocks for the chemical industry. Sustainable, biological production is constrained by production and recovery costs, including the need for intensive pH correction. Membrane electrolysis has been developed as an in situ extraction technology tailored to the direct rec...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0396-7

    authors: Andersen SJ,Candry P,Basadre T,Khor WC,Roume H,Hernandez-Sanabria E,Coma M,Rabaey K

    更新日期:2015-12-21 00:00:00

  • Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002.

    abstract:BACKGROUND:The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strai...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-014-0154-2

    authors: Therien JB,Zadvornyy OA,Posewitz MC,Bryant DA,Peters JW

    更新日期:2014-10-18 00:00:00

  • Pre-concentration of microalga Euglena gracilis by alkalescent pH treatment and flocculation mechanism of Ca3(PO4)2, Mg3(PO4)2, and derivatives.

    abstract:Background:Microalgae are widely be used in carbon sequestration, food supplements, natural pigments, polyunsaturated fatty acids, biofuel applications, and wastewater treatment. However, the difficulties incurred in algae cell separation and harvesting, and the exorbitant cost required to overcome these challenges, ar...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-020-01734-8

    authors: Wu M,Li J,Qin H,Lei A,Zhu H,Hu Z,Wang J

    更新日期:2020-05-29 00:00:00

  • A novel population balance model for the dilute acid hydrolysis of hemicellulose.

    abstract:BACKGROUND:Acid hydrolysis is a popular pretreatment for removing hemicellulose from lignocelluloses in order to produce a digestible substrate for enzymatic saccharification. In this work, a novel model for the dilute acid hydrolysis of hemicellulose within sugarcane bagasse is presented and calibrated against experim...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0211-5

    authors: Greenwood AA,Farrell TW,Zhang Z,O'Hara IM

    更新日期:2015-02-19 00:00:00

  • Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse.

    abstract:BACKGROUND:The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for their commercial application. In this study, we quantitatively compared the lignocellulolytic enzyme p...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-6-172

    authors: Marx IJ,van Wyk N,Smit S,Jacobson D,Viljoen-Bloom M,Volschenk H

    更新日期:2013-11-29 00:00:00

  • Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp. strain PCC 7002 from an oceanic environment.

    abstract:BACKGROUND:Oxygenic photosynthetic microorganisms such as cyanobacteria and microalgae have attracted attention as an alternative carbon source for the next generation of biofuels. Glycogen abundantly accumulated in cyanobacteria is a promising feedstock which can be converted to ethanol through saccharification and fe...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/1754-6834-7-88

    authors: Aikawa S,Nishida A,Ho SH,Chang JS,Hasunuma T,Kondo A

    更新日期:2014-06-11 00:00:00

  • Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis.

    abstract:BACKGROUND:Accumulation of recalcitrant oligosaccharides during high-solids loading enzymatic hydrolysis of cellulosic biomass reduces biofuel yields and increases processing costs for a cellulosic biorefinery. Recalcitrant oligosaccharides in AFEX-pretreated corn stover hydrolysate accumulate to the extent of about 18...

    journal_title:Biotechnology for biofuels

    pub_type: 杂志文章

    doi:10.1186/s13068-015-0378-9

    authors: Xue S,Uppugundla N,Bowman MJ,Cavalier D,Da Costa Sousa L,E Dale B,Balan V

    更新日期:2015-11-26 00:00:00