当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF PHARMACEUTICS期刊下所有文献 > Improved formulation of cationic solid lipid nanoparticles displays cellular uptake and biological activity of nucleic acids.

Improved formulation of cationic solid lipid nanoparticles displays cellular uptake and biological activity of nucleic acids.

Abstract：

:Non-viral delivery using cationic solid lipid nanoparticles (SLNs) represents a useful strategy to introduce large DNA and RNA molecules to target cells. A careful selection of components and their amounts is critical to improve transfection efficiency. In this work, a selected and optimized formulation of SLNs was used to efficiently transfect circular DNA and linear RNA molecules into cells. We characterized the main physicochemical characteristics and binding capabilities of these SLNs and show that they deliver DNA and RNA molecules into cells where they display full bioactivity at nontoxic concentrations using fluorescence- and luminescence-based methodologies. Hence, we established a novel and simple SLN formulation as a powerful tool for future therapeutic use.

journal_name

Int J Pharm

journal_title

International journal of pharmaceutics

authors

Fàbregas A,Prieto-Sánchez S,Suñé-Pou M,Boyero-Corral S,Ticó JR,García-Montoya E,Pérez-Lozano P,Miñarro M,Suñé-Negre JM,Hernández-Munain C,Suñé C

doi

10.1016/j.ijpharm.2016.11.026

subject

Has Abstract

pub_date

2017-01-10 00:00:00

pages

39-44

issue

1-2

eissn

0378-5173

issn

1873-3476

pii

S0378-5173(16)31078-X

journal_volume

516

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF PHARMACEUTICS文献大全
  • Amino Acid-functionalized hollow mesoporous silica nanospheres as efficient biocompatible drug carriers for anticancer applications.

    abstract：:Herein, a series of new amino acid-functionalized hollow mesoporous silica nanospheres (HMSNs) by post-grafting methods were prepared. These new materials were characterized by different techniques and were studied as matrices for the antineoplastic drug (cisplatin) transport and delivery. The results demonstrate that...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.118709

    authors： Ezzati N,Mahjoub AR,Abolhosseini Shahrnoy A,Syrgiannis Z

    更新日期：2019-12-15 00:00:00

  • Stabilization of gene delivery systems by freeze-drying.

    abstract：:Freeze-drying of three different forms of gene delivery systems was performed using a controlled two-step drying process and 10% sucrose as lyoprotectant. Complexes of pCMVL plasmid with transferrin-conjugated polyethylenimine, adenovirus-enhanced transferrinfection consisting of pCMVL/transferrin-polylysine complexes...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(97)00244-5

    authors： Talsma H,Cherng J,Lehrmann H,Kursa M,Ogris M,Hennink WE,Cotten M,Wagner E

    更新日期：1997-11-28 00:00:00

  • Effect of aqueous solubility of grafted moiety on the physicochemical properties of poly(d,l-lactide) (PLA) based nanoparticles.

    abstract：:In order to evaluate the solubility effect of grafted moiety on the physicochemical properties of poly(d,l-lactide) (PLA) based nanoparticles (NPs), two materials of completely different aqueous solubility, polyethylene glycol (PEG) and palmitic acid were grafted on PLA backbone at nearly the same grafting density, 2....

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.12.059

    authors： Essa S,Rabanel JM,Hildgen P

    更新日期：2010-03-30 00:00:00

  • Novel drug delivery strategies for improving econazole antifungal action.

    abstract：:Econazole is a commonly used azole antifungal in clinical treatment of superficial fungal infections. It is generally used as conventional cream and gel preparations under the brand names of Spectazole (United States), Ecostatin (Canada), Pevaryl (Western Europe). Treatment efficiency of antifungal drugs depends on th...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijpharm.2015.09.015

    authors： Firooz A,Nafisi S,Maibach HI

    更新日期：2015-11-10 00:00:00

  • Improvement of the intestinal membrane permeability of low molecular weight heparin by complexation with stem bromelain.

    abstract：:The aim of this study was to investigate the influence of the proteolytic enzyme bromelain on the permeation of heparin across the gastrointestinal epithelial barrier. Stability of the complex and effect of heparin on the enzymatic activity of bromelain was analysed photometrically by measuring bromelain enzymatic act...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2006.06.042

    authors： Grabovac V,Bernkop-Schnürch A

    更新日期：2006-12-01 00:00:00

  • Lubrication properties of potential alternative lubricants, glycerin fatty acid esters, to magnesium stearate.

    abstract：:To study the usefulness of glycerin fatty acid ester Poem TR-FB (TR-FB) and Poem TR-HB (TR-HB) as lubricants, pressure transmission ratio, ejection force, disintegration time, and tensile strength were measured at different concentrations and mixing times for granules and tablets. When each lubricant was mixed at 0.1-...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.11.001

    authors： Uchimoto T,Iwao Y,Ikegami Y,Murata T,Sonobe T,Miyagishima A,Itai S

    更新日期：2010-02-15 00:00:00

  • Properties of solid dispersions of piroxicam in polyvinylpyrrolidone.

    abstract：:Solid dispersions of piroxicam were prepared with polyvinylpyrrolidone (PVP) K-17 PF and PVP K-90 by solvent method. The physical state and drug:PVP interaction of solid dispersions and physical mixtures were characterized by X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy and differential scanning c...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(99)00070-8

    authors： Tantishaiyakul V,Kaewnopparat N,Ingkatawornwong S

    更新日期：1999-04-30 00:00:00

  • Public engagement workshop: how to improve medicines for older people?

    abstract：:Public engagement in medication management has become more and more important in promoting population health. A public engagement workshop attended by 78 members of the geriatric community, family carers as well as professionals from academic research, industry and regulatory agencies entitled 'How to improve medicine...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2013.11.024

    authors： Orlu-Gul M,Raimi-Abraham B,Jamieson E,Wei L,Murray M,Stawarz K,Stegemann S,Tuleu C,Smith FJ

    更新日期：2014-01-01 00:00:00

  • Size-dependency of nanoparticle-mediated gene transfection: studies with fractionated nanoparticles.

    abstract：:Nanoparticles formulated from biodegradable polymers such as poly (lactic acid) and poly (D,L-lactide-co-glycolide) (PLGA) are being extensively investigated as non-viral gene delivery systems due to their sustained release characteristics and biocompatibility. PLGA nanoparticles for DNA delivery are mainly formulated...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(02)00315-0

    authors： Prabha S,Zhou WZ,Panyam J,Labhasetwar V

    更新日期：2002-09-05 00:00:00

  • Tensile and shear methods for measuring strength of bilayer tablets.

    abstract：:Both shear and tensile measurement methods have been used to quantify interfacial bonding strength of bilayer tablets. The shear method is more convenient to perform, but reproducible strength data requires careful control of the placement of tablet and contact point for shear force application. Moreover, data obtaine...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.03.010

    authors： Chang SY,Li JX,Sun CC

    更新日期：2017-05-15 00:00:00

  • An apolipoprotein E modified liposomal nanoparticle: ligand dependent efficiency as a siRNA delivery carrier for mouse-derived brain endothelial cells.

    abstract：:A disorder in the brain endothelium is thought to be closely related to the pathophysiology of brain diseases. A method for delivering nucleic acids (i.e. short interference RNA; siRNA) to the brain endothelium should be an attractive strategy for curing brain disorders. A liposomal nanoparticle containing a proton-io...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2014.02.016

    authors： Tamaru M,Akita H,Kajimoto K,Sato Y,Hatakeyama H,Harashima H

    更新日期：2014-04-25 00:00:00

  • Edetate calcium disodium nanoparticle dry powder inhalation: a novel approach against heavy metal decorporation.

    abstract：:Objective was to develop and characterize nano-edetate calcium disodium (Ca-Na(2)EDTA) dry powder inhaler (DPI), and assess its in vitro and in vivo deposition using pharmacoscintigraphy techniques. Factors influencing nanoparticle formation including concentration of drug, polymer solution and stirring rate were dete...

    journal_title：International journal of pharmaceutics

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.ijpharm.2011.06.038

    authors： Kumar N,Soni S,Jaimini A,Ahmad FJ,Bhatnagar A,Mittal G

    更新日期：2011-09-15 00:00:00

  • Control, communication and monitoring of intravaginal drug delivery in dairy cows.

    abstract：:We present the design of an electronically controlled drug delivery system. The intravaginally located device is a low-invasive platform that can measure and react inside the cow vagina while providing external control and monitoring ability. The electronics manufactured from off the shelf components occupies 16 mL of...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2004.05.023

    authors： Cross PS,Künnemeyer R,Bunt CR,Carnegie DA,Rathbone MJ

    更新日期：2004-09-10 00:00:00

  • Chitosan/o-carboxymethyl chitosan nanoparticles for efficient and safe oral anticancer drug delivery: in vitro and in vivo evaluation.

    abstract：:The present study investigated the ability of a polyelectrolyte complex (CS/CMCS-NPs), composed of chitosan (CS) and o-carboxymeymethy chitosan (CMCS) as a pH responsive carrier for oral delivery of doxorubicin hydrochloride (DOX). The obtained CS/CMCS-NPs were characterized for various parameters including morphology...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2013.07.079

    authors： Feng C,Wang Z,Jiang C,Kong M,Zhou X,Li Y,Cheng X,Chen X

    更新日期：2013-11-30 00:00:00

  • Aggregation behavior of self-assembling polylactide/poly(ethylene glycol) micelles for sustained drug delivery.

    abstract：:A series of diblock copolymers were synthesized by ring-opening polymerization of l- or d-lactide in the presence of monomethoxy poly(ethylene glycol) (mPEG) with molar masses of 2000 and 5000. The aggregation behavior of the resulting water soluble PLA/PEG diblock copolymers in aqueous medium was studied with dynamic...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.04.030

    authors： Yang L,Qi X,Liu P,El Ghzaoui A,Li S

    更新日期：2010-07-15 00:00:00

  • Synthesis and evaluation of styrene-maleic acid copolymer conjugated amphotericin B.

    abstract：:Amphotericin B (AmB), which plays a central role in the treatment of systemic fungal infections, is difficult to formulate because it's sparingly soluble in water and organic solvents. We previously prepared AmB-loaded micelles using styrene-maleic acid copolymer (SMA). Although solubilization was achieved by this for...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.118719

    authors： Banshoya K,Kaneo Y,Tanaka T,Yamamoto S,Maeda H

    更新日期：2019-12-15 00:00:00

  • Effect of cholesterol and temperature on the elastic properties of niosomal membranes.

    abstract：:The mechanical characteristics of non-ionic bilayer membranes composed of sorbitan monostearate, cholesterol and poly-24-oxyethylene cholesteryl were studied by measuring the modulus of surface elasticity (mu), a measure of membrane strength, as a function of cholesterol content and temperature. The modulus of surface...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2005.05.009

    authors： Nasseri B

    更新日期：2005-08-26 00:00:00

  • Evaluation of the bioequivalence of capsules containing 150 mg of fluconazole.

    abstract：:Fluconazole is an antifungal agent. The purpose of this study was to evaluate bioequivalence of two commercial 150 mg capsule formulations of fluconazole available in the Brazilian market. The study was an open, randomized, two-period, two-group crossover trial with a 2-week washout interval. Blood samples were collec...

    journal_title：International journal of pharmaceutics

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/j.ijpharm.2004.09.013

    authors： Porta V,Chang KH,Storpirtis S

    更新日期：2005-01-06 00:00:00

  • A perivascular system releasing sirolimus prevented intimal hyperplasia in a rabbit model in a medium-term study.

    abstract：:The main complication of aortocoronary reconstruction with vein grafts is restenosis in the course of time. The aim was to assess the effect of a periadventitial polyester mesh releasing sirolimus on intimal hyperplasia of autologous grafts. We implanted v. jugularis ext. into a. carotis communis in rabbits. The vein ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.02.023

    authors： Skalský I,Szárszoi O,Filová E,Pařízek M,Lytvynets A,Malušková J,Lodererová A,Brynda E,Lisá V,Burdíková Z,Capek M,Pirk J,Bačáková L

    更新日期：2012-05-10 00:00:00

  • Multiscale study on the enhancing effect and mechanism of borneolum on transdermal permeation of drugs with different log P values and molecular sizes.

    abstract：:D-borneolum is commonly used as a permeation enhancer in Traditional Chinese Medicine (TCM) formulas for transdermal application. Additionally, two other sources of borneolums were recorded in the 2015 edition of the Chinese Pharmacopoeia (ChP), including L-borneolum and borneolum syntheticum. To guide the selection a...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.119225

    authors： Yang C,Guo S,Wu X,Yang P,Han L,Dai X,Shi X

    更新日期：2020-04-30 00:00:00

  • Designed sponges based on chitosan and cyclodextrin polymer for a local release of ciprofloxacin in diabetic foot infections.

    abstract：:Diabetic foot infections are the most common complications requiring hospitalisation of patients with diabetes. They often result in amputation to extremities and are associated with high morbi-mortality rates, especially when bone is infected. Treatment of these complications is based on surgical procedures, nursing ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.119677

    authors： Gauzit Amiel A,Palomino-Durand C,Maton M,Lopez M,Cazaux F,Chai F,Neut C,Foligné B,Martel B,Blanchemain N

    更新日期：2020-09-25 00:00:00

  • Formulation and evaluation of diclofenac sodium buccoadhesive discs.

    abstract：:Twenty diclofenac sodium buccoadhesive discs containing Cp974p, polycarbophil, PEO, SCMC-medium viscosity (SCMC-MV), SCMC-ultrahigh viscosity (SCMC-UHV) or their combinations were prepared. These buccoadhesive discs were evaluated for release pattern, swelling capacity, surface pH, mucoadhesion performance, and in vit...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2004.07.033

    authors： El-Samaligy MS,Yahia SA,Basalious EB

    更新日期：2004-11-22 00:00:00

  • Microemulsion-based hydrogel formulation of ibuprofen for topical delivery.

    abstract：:The purpose of this study was to construct microemulsion-base hydrogel formulation for topical delivery of ibuprofen. Ethyl oleate (EO) was screened as the oil phase of microemulsions, due to a good solubilizing capacity of the microemulison systems and excellent skin permeation rate of ibuprofen. The pseudo-ternary p...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2006.02.015

    authors： Chen H,Chang X,Du D,Li J,Xu H,Yang X

    更新日期：2006-06-06 00:00:00

  • A novel remote controlled capsule for site-specific drug delivery in human GI tract.

    abstract：:Remote controlled capsule (RCC) has been extensively used in the field of site-specific drug delivery. It is a potent tool to study the regional drug absorption of the gastrointestinal (GI) tract that provides pharmaceutical scientists with significant pharmacokinetics data for oral drug formulation development. In pr...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.08.026

    authors： Xitian P,Hongying L,Kang W,Yulin L,Xiaolin Z,Zhiyu W

    更新日期：2009-12-01 00:00:00

  • In vivo gene transfection via intravitreal injection of cationic liposome/plasmid DNA complexes in rabbits.

    abstract：:To optimize the in vivo ocular transfection efficiency of plasmid DNA (pDNA)/cationic liposome complexes, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA)/dioleoylphosphatidylethanolamine (DOPE) (1:1 molar ratio) liposomes and DOTMA/cholesterol (Chol) (1:1 molar ratio) liposomes were prepared with...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2004.03.013

    authors： Kawakami S,Harada A,Sakanaka K,Nishida K,Nakamura J,Sakaeda T,Ichikawa N,Nakashima M,Sasaki H

    更新日期：2004-07-08 00:00:00

  • Fabrication of inhalable spore like pharmaceutical particles for deep lung deposition.

    abstract：:An innovative strategy of fabricating uniform spore like drug particles to improve pulmonary drug delivery efficiency was disclosed in the present study. Spore like particles were prepared through combination of high gravity controlled precipitation and spray drying process with insulin as model drug first, showing ro...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.03.044

    authors： Shen ZG,Chen WH,Jugade N,Gao LY,Glover W,Shen JY,Yun J,Chen JF

    更新日期：2012-07-01 00:00:00

  • Characterization of nifedipine microparticles prepared by hot air coating technique.

    abstract：:In the present work, the Hot Air Coating (HAC) technique was used to prepare microparticulate systems containing nifedipine. Binary mixtures constituting of nifedipine and cetearyl alcohol (CA) in different proportions (30:70, 50:50, 70:30) were studied: they were homogenized by mixing or milling before spray treatmen...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2005.01.010

    authors： Giovannelli L,Bellomi S,Pattarino F,Albertini B

    更新日期：2005-04-11 00:00:00

  • Drug release modeled by dissolution, diffusion, and immobilization.

    abstract：:This article presents a novel drug release model that combines drug dissolution, diffusion, and immobilization caused by adsorption of the drug to the tablet constituents. Drug dissolution is described by the well-known Noyes-Whitney equation and drug adsorption by a Langmuir-Freundlich adsorption isotherm, and these ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(02)00539-2

    authors： Frenning G,Strømme M

    更新日期：2003-01-02 00:00:00

  • Porous clay heterostructures: A new inorganic host for 5-fluorouracil encapsulation.

    abstract：:This study proposed a new inorganic host for drug encapsulation. Porous clay heterostructure (PCH), synthesized using modified montmorillonite with hexadecyltrimethylammonium bromide, was used as host material and 5-fluorouracil (5-FU) as guest drug. Drug encapsulation within PCH in different conditions (soaking time,...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.05.053

    authors： Gârea SA,Mihai AI,Ghebaur A,Nistor C,Sârbu A

    更新日期：2015-08-01 00:00:00

  • A micelle-like structure of poloxamer-methotrexate conjugates as nanocarrier for methotrexate delivery.

    abstract：:The purpose of this study was to develop a novel featured and flexible methotrexate (MTX) formulation, in which MTX was physically entrapped and chemically conjugated in the same drug delivery system. A series of poloxamer-MTX (p-MTX) conjugates was synthesized, wherein MTX was grafted to poloxamer through an ester bo...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.04.014

    authors： Ren J,Fang Z,Yao L,Dahmani FZ,Yin L,Zhou J,Yao J

    更新日期：2015-06-20 00:00:00