Abstract:
:Freeze-drying of three different forms of gene delivery systems was performed using a controlled two-step drying process and 10% sucrose as lyoprotectant. Complexes of pCMVL plasmid with transferrin-conjugated polyethylenimine, adenovirus-enhanced transferrinfection consisting of pCMVL/transferrin-polylysine complexes linked to inactivated adenovirus particles, and a recombinant, E1-defective adenovirus expressing a luciferase reporter gene were tested. Three weeks after freeze-drying the reagents were rehydrated with water and tested for transfection activity. Luciferase gene expression levels were retained at high levels in all three systems, in contrast to reagents stored in solution. The use of the lyoprotectant was essential. In the absence of sucrose the transfection activities dropped by a factor of 100-1000. The data suggest freeze-drying as a useful method for stabilization and storage of standardized batches of transfection agents.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Talsma H,Cherng J,Lehrmann H,Kursa M,Ogris M,Hennink WE,Cotten M,Wagner Edoi
10.1016/s0378-5173(97)00244-5keywords:
subject
Has Abstractpub_date
1997-11-28 00:00:00pages
233-238issue
2eissn
0378-5173issn
1873-3476pii
S0378517397002445journal_volume
157pub_type
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