Variations of B cell subpopulations in peripheral blood of healthy Mexican population according to age: Relevance for diagnosis of primary immunodeficiencies.

Abstract:

BACKGROUND:Peripheral blood B cells include lymphocytes at various stages of differentiation, each with a specific function in the immune response. All these stages show variations in percentage and absolute number throughout human life. The numbers and proportions of B subpopulation are influenced by factors such as gender, age, ethnicity, and lifestyle. This study establishes reference values according to age of peripheral blood B cell subtypes in healthy Mexican population. METHODS:Peripheral blood from healthy new-borns and adults were analysed for total B cell subpopulations, using surface markers such as CD19, IgM, IgD, CD21, CD24, CD27, and CD38, to identify naïve, memory with and without isotype switch, double-negative, transitional, and plasmablast cells. RESULTS:We observed a significant variation in terms of frequency and absolute counts between all groups analysed. Values from each B cell subpopulation show variations according to age. CONCLUSIONS:In order to attempt to elucidate reference values for B cell subpopulation, the present study evaluated a population sample of healthy blood donors from this region. Values reported here can also be used as a tool for diagnosis of diseases in which B cell maturation is affected.

authors

Berrón-Ruíz L,López-Herrera G,Ávalos-Martínez CE,Valenzuela-Ponce C,Ramírez-SanJuan E,Santoyo-Sánchez G,Mújica Guzmán F,Espinosa-Rosales FJ,Santos-Argumedo L

doi

10.1016/j.aller.2016.05.003

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

571-579

issue

6

eissn

0301-0546

issn

1578-1267

pii

S0301-0546(16)30094-5

journal_volume

44

pub_type

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