Listeria monocytogenes encodes a functional ESX-1 secretion system whose expression is detrimental to in vivo infection.

Abstract:

:Bacterial pathogenicity deeply depends on the ability to secrete virulence factors that bind specific targets on host cells and manipulate host responses. The Gram-positive bacterium Listeria monocytogenes is a human foodborne pathogen that remains a serious public health concern. To transport proteins across its cell envelope, this facultative intracellular pathogen engages a set of specialized secretion systems. Here we show that L. monocytogenes EGDe uses a specialized secretion system, named ESX-1, to secrete EsxA, a homolog of the virulence determinants ESAT-6 and EsxA of Mycobacterium tuberculosis and Staphylococcus aureus, respectively. Our data show that the L. monocytogenes ESX-1 secretion system and its substrates are dispensable for bacterial invasion and intracellular multiplication in eukaryotic cell lines. Surprisingly, we found that the EssC-dependent secretion of EsxA has a detrimental effect on L. monocytogenes in vivo infection.

journal_name

Virulence

journal_title

Virulence

authors

Pinheiro J,Reis O,Vieira A,Moura IM,Zanolli Moreno L,Carvalho F,Pucciarelli MG,García-Del Portillo F,Sousa S,Cabanes D

doi

10.1080/21505594.2016.1244589

subject

Has Abstract

pub_date

2017-08-18 00:00:00

pages

993-1004

issue

6

eissn

2150-5594

issn

2150-5608

journal_volume

8

pub_type

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