Abstract:
:Rift Valley fever is a mosquito-borne zoonotic disease affecting ruminants and humans. Rift Valley fever virus (RVFV: family Bunyaviridae, genus Phlebovirus) causes abortions and fetal malformations in ruminants, and hemorrhagic fever, encephalitis, or retinitis in humans. The live-attenuated MP-12 vaccine is conditionally licensed for veterinary use in the US. However, this vaccine lacks a marker for the differentiation of vaccinated from infected animals (DIVA). NSs gene is dispensable for RVFV replication, and thus, rMP-12 strains lacking NSs gene is applicable to monitor vaccinated animals. However, the immunogenicity of MP-12 lacking NSs was not as high as parental MP-12. Thus, chimeric MP-12 strains encoding NSs from either Toscana virus (TOSV), sandfly fever Sicilian virus (SFSV) or Punta Toro virus Adames strain (PTA) were characterized previously. Although chimeric MP-12 strains are highly immunogenic, the attenuation through the S-segment remains unknown. Using pathogenic ZH501 strain, we aimed to demonstrate the attenuation of ZH501 strain through chimeric S-segment encoding either the NSs of TOSV, SFSV, PTA, or Punta Toro virus Balliet strain (PTB). In addition, we characterized rZH501 encoding a human dominant-negative PKR (PKRΔE7), which also enhances the immunogenicity of MP-12. Study done on mice revealed that attenuation of rZH501 occurred through the S-segment encoding either PKRΔE7 or SFSV NSs. However, rZH501 encoding either TOSV, PTA, or PTB NSs in the S-segment uniformly caused lethal encephalitis. Our results indicated that the S-segments encoding PKRΔE7 or SFSV NSs are attenuated and thus applicable toward next generation MP-12 vaccine candidates that encode a DIVA marker.
journal_name
Virulencejournal_title
Virulenceauthors
Nishiyama S,Slack OA,Lokugamage N,Hill TE,Juelich TL,Zhang L,Smith JK,Perez D,Gong B,Freiberg AN,Ikegami Tdoi
10.1080/21505594.2016.1195528subject
Has Abstractpub_date
2016-11-16 00:00:00pages
871-881issue
8eissn
2150-5594issn
2150-5608journal_volume
7pub_type
杂志文章相关文献
Virulence文献大全abstract::Biofilm-degrading enzymes could be used for the gentle cleaning of industrial and medical devices and the manufacture of biofilm-resistant materials. We therefore investigated 20 species and strains of the bacterial genus Lysobacter for their ability to degrade experimental biofilms formed by Staphylococcus epidermidi...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.27919
更新日期:2014-04-01 00:00:00
abstract::The exploration of epidemic dynamics on dynamically evolving ("adaptive") networks poses nontrivial challenges to the modeler, such as the determination of a small number of informative statistics of the detailed network state (that is, a few "good observables") that usefully summarize the overall (macroscopic, system...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1121357
更新日期:2016-01-01 00:00:00
abstract::Invasive pulmonary aspergillosis (IPA) is a severe infection that is difficult to diagnose due to the ubiquitous presence of fungal spores, the underlying diseases of risk patients, and limitations of currently available markers. In this study, we performed a comprehensive liquid chromatography tandem mass spectrometr...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1824960
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Hypervirulent Klebsiella pneumoniae(hvKp) is an increasingly important pathogen. Tracking its epidemiology and evolving antimicrobial resistance will facilitate care. METHODS:A retrospective study was conducted in two hospitals. We collected the clinical data. Antimicrobial and virulence-associated phenotyp...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1809322
更新日期:2020-12-01 00:00:00
abstract::Anthrax toxins and capsule are the major virulence factors of Bacillus anthracis. They are encoded by genes located on the plasmids pXO1 and pXO2, respectively. The vaccine strain Pasteur II was produced from high temperature subcultures of B. anthracis, which resulted in virulence attenuation through the loss of the ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1164366
更新日期:2016-07-03 00:00:00
abstract::Bacterial pathogenicity deeply depends on the ability to secrete virulence factors that bind specific targets on host cells and manipulate host responses. The Gram-positive bacterium Listeria monocytogenes is a human foodborne pathogen that remains a serious public health concern. To transport proteins across its cell...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1244589
更新日期:2017-08-18 00:00:00
abstract::In previous studies we demonstrated that the staphylococcal α-toxin inhibits adhesion and invasion of S. aureus by epithelial cells through binding to α5β1 integrin, a receptor of fibronectin. Moreover, we revealed that a H35A mutation abolishes the cytotoxicity of α-toxin completely. These findings led us to hypothes...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.22719
更新日期:2013-01-01 00:00:00
abstract::The lipopolysaccharide (LPS) is a major virulence factor of Brucella, a facultative intracellular pathogenic Gram-negative bacterium. Brucella LPS exhibits a low toxicity and its atypical structure was postulated to delay the host immune response, favouring the establishment of chronic disease. Here we carried out an ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2017.1386831
更新日期:2018-01-01 00:00:00
abstract::Legionella pneumophila is the primary respiratory pathogen and mostly transmitted to human through water cooling systems and cause mild to severe pneumonia with high mortality rate especially in elderly both in hospitals and community. However, current Legionella risk assessments may be compromised by uncertainties in...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1170944
更新日期:2016-07-03 00:00:00
abstract::Mucormycosis is an uncommon fungal infection that has been increasingly reported in severely immunocompromised patients receiving Aspergillus-active antifungals. Although clinical studies and pre-clinical animal models have suggested a unique predisposition for breakthrough mucormycoses in patients receiving voriconaz...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.2.4.17074
更新日期:2011-07-01 00:00:00
abstract::Human herpesviruses have latency and lytic replication phases in their lifecycle. Proper regulation of herpesviral lifecycle is essential for the evasion of host immune surveillance and development of their related diseases. Recent advancements indicate a role of a novel class of viral non-coding RNAs, microRNA (miRNA...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.1.5.12966
更新日期:2010-09-01 00:00:00
abstract::Aeromonas hydrophila (A. hydrophila) can cause a number of diseases in both human and animals. A. hydrophila-related infections in aquaculture cause severe economic losses every year throughout the world. The emergence of antibiotic resistance that is due to the abuse of antibiotics has limited the application of anti...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1867455
更新日期:2021-12-01 00:00:00
abstract::The emergence of resistance requires alternative methods to treat Candida albicans infections. We evaluated efficacy of the efflux pump inhibitor (EPI) verapamil (VER) with fluconazole (FLC) against FLC-resistant (CaR) and -susceptible C. albicans (CaS). The susceptibility of both strains to VER and FLC was determined...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1868814
更新日期:2021-12-01 00:00:00
abstract::Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma. H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes. The aim of the presen...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2018.1532243
更新日期:2018-01-01 00:00:00
abstract::Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) have been reported in recent years across Asian countries and pose a serious threat to public health. Neutrophils represent the first line of defense against numerous infectious pathogens, such as CR-hvKP. Neutrophil extracellular traps (NETs) constitu...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1809325
更新日期:2020-12-01 00:00:00
abstract::Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1855912
更新日期:2020-12-01 00:00:00
abstract::The hemagglutinin (HA) proteins derived from avian influenza viruses bind specifically to the α2-3 sialoglycan (Sia glycan), whereas human-adapted influenza viruses prefer to bind to the α2-6 Sia glycan. A switch of glycan specificity from α2-3 Sia glycan to α2-6 Sia glycan appears to be critical for a virus to become...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.21822
更新日期:2012-08-15 00:00:00
abstract::Particle size dictates where aerosolized pathogens deposit in the respiratory tract, thereafter the pathogens potential to cause disease is influenced by tissue tropism, clearance kinetics and the host immunological response. This interplay brings pathogens into contact with a range of tissues spanning the respiratory...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.27172
更新日期:2013-11-15 00:00:00
abstract::Sepsis remains a common, serious, and heterogeneous clinical entity that is difficult to define adequately. Despite its importance as a public health problem, efforts to develop and gain regulatory approval for a specific therapeutic agent for the adjuvant treatment of sepsis have been remarkably unsuccessful. One ste...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.26083
更新日期:2014-01-01 00:00:00
abstract::Non-invasive imaging techniques in microbial disease models have delivered valuable insights in the intimate pathogen-host interplay during infection. Here we describe evaluation and validation of a transgenic bioluminescence reporter strain of the human-pathogenic mold Aspergillus fumigatus, one of the main fungal pa...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.3.1.18799
更新日期:2012-01-01 00:00:00
abstract::Daptomycin resistance (DAP(R)) in Staphylococcus aureus is associated with mutations in genes that are also implicated in staphylococcal pathogenesis. Using a laboratory-derived series of DAP exposed strains, we showed a relationship between increasing DAP MIC and reduced virulence in a Galleria mellonella infection m...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1011532
更新日期:2015-01-01 00:00:00
abstract::Cryptococcus neoformans: (H99W) was serially passaged in the invertebrate wax moth Galleria mellonella fifteen times to study how fungal virulence evolves under selection and whether those adaptations affect virulence. The G. mellonella passaged strain (P15) and the pre-passage H99W strains were used to infect three d...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1831332
更新日期:2020-12-01 00:00:00
abstract::Periodontitis is an infectious inflammatory disease that destroys the tooth-supporting tissues. It is caused by the formation of subgingival biofilms on the surface of the tooth. Characteristic bacteria associated with subgingival biofilms are the Gram-negative anaerobes Porphyromonas gingivalis, Tannerella forsythia ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1081731
更新日期:2015-01-01 00:00:00
abstract::Several human pathogens bind and respond to host cytokines, which can be considered a virulence mechanism that communicates defensive actions of the host to the pathogen. This review summarizes the current knowledge of bacterial cytokine-binding proteins, with a particular focus on their functional and structural char...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2017.1363140
更新日期:2017-11-17 00:00:00
abstract::In all eukaryotic cells, the ER stress response is pivotal to survival and adaptation under stress conditions. During temperature adaptation in the human fungal pathogen Cryptococcus neoformans, ER stress is engaged transiently. Studies of this response have demonstrated that both the engagement (turning on the respon...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.27187
更新日期:2014-02-15 00:00:00
abstract::Bacterial IgA1 proteases are thought to be important virulence factors in respiratory tract infections. These proteolytic enzymes specifically cleave one of several post-proline peptide bonds within the hinge region of human immunoglobulin A1 (IgA1). Human IgA1 was cleaved with an IgA1 protease purified from an isolat...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.2.2.15401
更新日期:2011-03-01 00:00:00
abstract::Bacterial eukaryotic-like serine threonine kinases (eSTKs) and serine threonine phosphatases (eSTPs) have emerged as important signaling elements that are indispensable for pathogenesis. Differing considerably from their histidine kinase counterparts, few eSTK genes are encoded within the average bacterial genome, and...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/21505594.2014.983404
更新日期:2014-01-01 00:00:00
abstract::In the present study, we examined prolonged infection after antigenic co-stimulation by inoculation of the fungus Fonsecaea pedrosoi at two different sites in three mouse strains (BALB/c, Swiss, and C57BL/6). Using this murine model of infection, we showed that antigen induction of infection at more than one site led ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.1.1.9920
更新日期:2010-01-01 00:00:00
abstract::Quorum-sensing (QS), the regulation of bacterial gene expression in response to changes in cell density, involves pathways that synthesize signaling molecules (auto-inducers). The luxS/AI-2-mediated QS system has been identified in both gram-positive and gram-negative bacteria. Bacillus anthracis, the etiological agen...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.1.2.10752
更新日期:2010-03-01 00:00:00
abstract::We describe a young woman with profound anemia whose serum iron studies were incongruous with what we expected from iron deficiency anemia. Her high serum iron was not fully explainable until we examined the patient and noticed a large black tattoo on her left flank area. Apparently iron oxide in the ink used for the ...
journal_title:Virulence
pub_type: 信件
doi:10.4161/viru.22122
更新日期:2012-11-15 00:00:00