Abstract:
:The virulence behaviors of many Gram-negative bacterial pathogens are governed by quorum-sensing (QS), a hierarchical system of gene regulation that relies on population density by producing and detecting extracellular signaling molecules. Although extensively studied under in vitro conditions, adaptation of QS system to physiologically relevant host environment is not fully understood. In this study, we investigated the influence of lung environment on the regulation of Pseudomonas aeruginosa virulence factors by QS in a mouse model of acute pneumonia. When cultured under laboratory conditions in lysogeny broth, wild-type P. aeruginosa strain PAO1 began to express QS-regulated virulence factors elastase B (LasB) and rhamnolipids (RhlA) during transition from late-exponential into stationary growth phase. In contrast, during acute pneumonia as well as when cultured in mouse bronchial alveolar lavage fluids (BALF), exponential phase PAO1 bacteria at low population density prematurely expressed QS regulatory genes lasI-lasR and rhlI-rhlR and their downstream virulence genes lasB and rhlA. Further analysis indicated that surfactant phospholipids were the primary components within BALF that induced the synthesis of N-(3-oxododecanoyl)-L-homoserine lactone (C12-HSL), which triggered premature expression of LasB and RhlA. Both phenol extraction and phospholipase A2 digestion abolished the ability of mouse BALF to promote LasB and RhlA expression. In contrast, provision of the major surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC) restored the expression of both virulence factors. Collectively, our study demonstrates P. aeruginosa modulates its QS to coordinate the expression of virulence factors during acute pneumonia by recognizing pulmonary surfactant phospholipids.
journal_name
Virulencejournal_title
Virulenceauthors
Kuang Z,Bennett RC,Lin J,Hao Y,Zhu L,Akinbi HT,Lau GWdoi
10.1080/21505594.2020.1809327subject
Has Abstractpub_date
2020-12-01 00:00:00pages
1090-1107issue
1eissn
2150-5594issn
2150-5608journal_volume
11pub_type
杂志文章相关文献
Virulence文献大全abstract::In previous studies we demonstrated that the staphylococcal α-toxin inhibits adhesion and invasion of S. aureus by epithelial cells through binding to α5β1 integrin, a receptor of fibronectin. Moreover, we revealed that a H35A mutation abolishes the cytotoxicity of α-toxin completely. These findings led us to hypothes...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.22719
更新日期:2013-01-01 00:00:00
abstract::Pathogenic bacteria produce virulence factors called effectors, which are important components of the infection process. Effectors aid in pathogenesis by facilitating bacterial attachment, pathogen entry into or exit from the host cell, immunoevasion, and immunosuppression. Effectors also have the ability to subvert h...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.29091
更新日期:2014-01-01 00:00:00
abstract::Sepsis remains a common, serious, and heterogeneous clinical entity that is difficult to define adequately. Despite its importance as a public health problem, efforts to develop and gain regulatory approval for a specific therapeutic agent for the adjuvant treatment of sepsis have been remarkably unsuccessful. One ste...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.26083
更新日期:2014-01-01 00:00:00
abstract::The hemagglutinin (HA) proteins derived from avian influenza viruses bind specifically to the α2-3 sialoglycan (Sia glycan), whereas human-adapted influenza viruses prefer to bind to the α2-6 Sia glycan. A switch of glycan specificity from α2-3 Sia glycan to α2-6 Sia glycan appears to be critical for a virus to become...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.21822
更新日期:2012-08-15 00:00:00
abstract::Lipid mediators, derived from arachidonic acid metabolism, play an important role in immune regulation. The functions of bioactive eicosanoids range from modulating cytokine signaling and inflammasome formation to anti-inflammatory and pro-resolving activities. Human pathogenic fungi such as Candida albicans, Candida ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2018.1475797
更新日期:2018-01-01 00:00:00
abstract::Maternal antibodies induced by vaccination during pregnancy cross the placental barrier and can close the susceptibility gap to pertussis in young infants up to the start of primary immunization. As not only the quantity but also the quality of circulating antibodies is important for protection, we assessed whether ma...
journal_title:Virulence
pub_type: 临床试验,杂志文章
doi:10.1080/21505594.2017.1296998
更新日期:2017-10-03 00:00:00
abstract::Daptomycin resistance (DAP(R)) in Staphylococcus aureus is associated with mutations in genes that are also implicated in staphylococcal pathogenesis. Using a laboratory-derived series of DAP exposed strains, we showed a relationship between increasing DAP MIC and reduced virulence in a Galleria mellonella infection m...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1011532
更新日期:2015-01-01 00:00:00
abstract::Gram-negative bacterial (GNB) infections are a leading cause of serious infections both in hospitals and the community. The mortality remains high despite potent antimicrobials and modern supportive care. In the last decade invasive GNB have become increasingly resistant to commonly used antibiotics, and attempts to i...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.25965
更新日期:2014-01-01 00:00:00
abstract::The emergence of multiresistant Gram-negative bacteria requires new therapies for combating bacterial infections. Targeting the biogenesis of virulence factors could be an alternative strategy instead of killing bacteria with antibiotics. The outer membrane (OM) of Gram-negative bacteria acts as a physical barrier. At...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1278333
更新日期:2017-10-03 00:00:00
abstract::The lipopolysaccharide (LPS) is a major virulence factor of Brucella, a facultative intracellular pathogenic Gram-negative bacterium. Brucella LPS exhibits a low toxicity and its atypical structure was postulated to delay the host immune response, favouring the establishment of chronic disease. Here we carried out an ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2017.1386831
更新日期:2018-01-01 00:00:00
abstract::Legionella pneumophila, the causative agent of Legionnaires' disease, is widely distributed throughout natural and artificial water systems and can replicate in macrophages and amoebae. Amoebae are the natural hosts of L. pneumophila, whereas macrophages are incidentally infected. The life cycle of L. pneumophila comp...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2017.1373925
更新日期:2018-01-01 00:00:00
abstract::Many of the alterations that affect innate and adaptive immune cell compartments in HIV-infected patients are reminiscent of the process of immune aging, characteristic of old age. These alterations define the immunological age of individuals and are likely to participate to the decline of immune competence with HIV d...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2016.1195536
更新日期:2017-07-04 00:00:00
abstract::Trehalose is a disaccharide formed from two glucose molecules. This sugar molecule can be isolated from a range of organisms including bacteria, fungi, plants and invertebrates. Trehalose has a variety of functions including a role as an energy storage molecule, a structural component of glycolipids and plays a role i...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1199316
更新日期:2017-01-02 00:00:00
abstract::Multi-antibiotic drug-resistant (MDR) gram-negative bacilli are becoming a major threat to the standard care of septic patients. Empiric antimicrobial drug regimens to cover likely bacterial pathogens have to be altered in keeping with the spread of MDR pathogens in the health care setting and in the community. Reliab...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.26210
更新日期:2014-01-01 00:00:00
abstract::Legionella pneumophila is the primary respiratory pathogen and mostly transmitted to human through water cooling systems and cause mild to severe pneumonia with high mortality rate especially in elderly both in hospitals and community. However, current Legionella risk assessments may be compromised by uncertainties in...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1170944
更新日期:2016-07-03 00:00:00
abstract::Drugs such as linezolid that inhibit bacterial protein synthesis may be beneficial in treating infections caused by toxigenic Staphylococcus aureus. As protein synthesis inhibitors have no effect on preformed toxins, neutralization of pathogenic exotoxins with anti-toxin antibodies may be beneficial in conjunction wit...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1267894
更新日期:2017-10-03 00:00:00
abstract::Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma. H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes. The aim of the presen...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2018.1532243
更新日期:2018-01-01 00:00:00
abstract::Francisella tularensis is a highly virulent bacterial pathogen that is easily aerosolized and has a low infectious dose. As an intracellular pathogen, entry of Francisella into host cells is critical for its survival and virulence. However, the initial steps of attachment and internalization of Francisella into host c...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.25629
更新日期:2013-11-15 00:00:00
abstract::The exploration of epidemic dynamics on dynamically evolving ("adaptive") networks poses nontrivial challenges to the modeler, such as the determination of a small number of informative statistics of the detailed network state (that is, a few "good observables") that usefully summarize the overall (macroscopic, system...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1121357
更新日期:2016-01-01 00:00:00
abstract::Entomopathogenic fungi are potential biological control agents of mosquitoes. Our group observed that not all mosquitoes were equally susceptible to fungal infection and observed significant differences in virulence of different spore types. Conidiospores and blastospores were tested against Culex quinquefasciatus lar...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2018.1509665
更新日期:2018-01-01 00:00:00
abstract::Mucormycosis is an uncommon fungal infection that has been increasingly reported in severely immunocompromised patients receiving Aspergillus-active antifungals. Although clinical studies and pre-clinical animal models have suggested a unique predisposition for breakthrough mucormycoses in patients receiving voriconaz...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.2.4.17074
更新日期:2011-07-01 00:00:00
abstract::Carbapenem-Resistant Enterobacteriaceae (CRE) are an emerging healthcare crisis. Infections due to CRE are associated with high morbidity and mortality, especially in critically ill patients. Due to the multi-drug resistant nature of these infections only limited treatment options are available. Antimicrobials that ha...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2016.1221021
更新日期:2017-05-19 00:00:00
abstract::A new hypervirulent (hypermucoviscous) variant of Klebsiella pneumoniae has emerged. First described in the Asian Pacific Rim, it now increasingly recognized in Western countries. Defining clinical features are the ability to cause serious, life-threatening community-acquired infection in younger healthy hosts, includ...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.22718
更新日期:2013-02-15 00:00:00
abstract::Viral infections of the respiratory tract can be complicated by bacterial superinfection, resulting in a significantly longer duration of illness and even a fatal outcome. In this review, we focused on interactions between S. aureus and non-influenza viruses. Clinical data evidenced that rhinovirus infection may incre...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2018.1504561
更新日期:2018-01-01 00:00:00
abstract::Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) have been reported in recent years across Asian countries and pose a serious threat to public health. Neutrophils represent the first line of defense against numerous infectious pathogens, such as CR-hvKP. Neutrophil extracellular traps (NETs) constitu...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1809325
更新日期:2020-12-01 00:00:00
abstract::Patients undergoing haematopoietic stem cell transplantation (HSCT) are highly exposed to infectious agents. However, it is not known why certain HSCT recipients rapidly develop severe infections while other, despite similar immunosuppressive conditions, do not. Increasing evidence suggests that such differences may b...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2016.1234566
更新日期:2016-11-16 00:00:00
abstract::Staphylococcus aureus infections are becoming a major global health issue due to the rapid emergence of multidrug-resistant strains. Therefore, there is an urgent need to develop an effective vaccine to prevent and control these infections. In order to develop a universal immunization strategy, we constructed a mutant...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2017.1417723
更新日期:2018-01-01 00:00:00
abstract::Pathogens frequently exist in an immunological balancing act with their host. Pathogens must not only replicate within a host but also transmit effectively between hosts to perpetuate their species. On the other hand, the host seeks to maintain homeostasis by clearing pathogens. The inflammasome is a multi-protein com...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.20266
更新日期:2012-05-01 00:00:00
abstract::Galleria mellonella (greater wax moth or honeycomb moth) has been introduced as an alternative model to study microbial infections. G. mellonella larvae can be easily and inexpensively obtained in large numbers and are simple to use as they don't require special lab equipment. There are no ethical constraints and thei...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2015.1135289
更新日期:2016-04-02 00:00:00
abstract::We recently developed a method that allows fast differentiation between Streptococcus pyogenes (GAS) strains. The method named phage profiling (PP) is based on a simple assumption that a regular PCR reaction with Taq polymerase and relatively short elongation time is not able to yield long DNA fragment, such as ~40-50...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.21887
更新日期:2012-10-01 00:00:00