Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin.

Abstract:

:Aeromonas hydrophila (A. hydrophila) can cause a number of diseases in both human and animals. A. hydrophila-related infections in aquaculture cause severe economic losses every year throughout the world. The emergence of antibiotic resistance that is due to the abuse of antibiotics has limited the application of antibiotics. Thus, novel approaches are needed to combat with treatment failure of antibiotics caused by resistant bacterial strains. Aerolysin plays a critical role in the pathogenesis of A. hydrophila and has been considered as a novel target for developing drugs based on anti-virulence strategies. Here, we reported that luteolin, a natural product with no anti-A. hydrophila activity, could reduce aerolysin-induced hemolysis by inhibiting aerolysin activity. The binding mode was simulated by molecular docking and dynamics simulation. Then the main binding sites were confirmed by fluorescence quenching assays. We found that luteolin could hindered the formation of functional heptamer of aerolysin according to the results of the oligomerization assay. Moreover, luteolin could protect A549 cells from aerolysin mediated cell death and increase the survival rate of A. hydrophila-infected channel catfish. These findings suggest a novel approach to developing drugs fighting against A. hydrophila, and luteolin can be a promising drug candidate for treatment of A. hydrophila-associated infections.

journal_name

Virulence

journal_title

Virulence

authors

Dong J,Zhang L,Liu Y,Xu N,Zhou S,Yang Y,Yang Q,Ai X

doi

10.1080/21505594.2020.1867455

subject

Has Abstract

pub_date

2021-12-01 00:00:00

pages

165-176

issue

1

eissn

2150-5594

issn

2150-5608

journal_volume

12

pub_type

杂志文章
  • Highly specific host-pathogen interactions influence Metarhizium brunneum blastospore virulence against Culex quinquefasciatus larvae.

    abstract::Entomopathogenic fungi are potential biological control agents of mosquitoes. Our group observed that not all mosquitoes were equally susceptible to fungal infection and observed significant differences in virulence of different spore types. Conidiospores and blastospores were tested against Culex quinquefasciatus lar...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2018.1509665

    authors: Alkhaibari AM,Lord AM,Maffeis T,Bull JC,Olivares FL,Samuels RI,Butt TM

    更新日期:2018-01-01 00:00:00

  • Ebolavirus VP35 is a multifunctional virulence factor.

    abstract::Ebola virus (EBOV) is a member of the filoviridae family that causes severe hemorrhagic fever during sporadic outbreaks, and no approved treatments are currently available. The multifunctional EBOV VP35 protein facilitates immune evasion by antagonizing antiviral signaling pathways and is important for viral RNA synth...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.4161/viru.1.6.12984

    authors: Leung DW,Prins KC,Basler CF,Amarasinghe GK

    更新日期:2010-11-01 00:00:00

  • TBX21-1993T/C (rs4794067) polymorphism is associated with increased risk of chronic periodontitis and increased T-bet expression in periodontal lesions, but does not significantly impact the IFN-g transcriptional level or the pattern of periodontophatic b

    abstract::Th1-polarized host response, mediated by IFN-γ, has been associated with increased severity of periodontal disease as well as control of periodontal infection. The functional polymorphism TBX21-1993T/C (rs4794067) increases the transcriptional activity of the TBX21 gene (essential for Th1 polarization) resulting in a ...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2015.1029828

    authors: Cavalla F,Biguetti CC,Colavite PM,Silveira EV,Martins W Jr,Letra A,Trombone AP,Silva RM,Garlet GP

    更新日期:2015-01-01 00:00:00

  • A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8.

    abstract::Intrinsically disordered proteins (IDPs) do not have a well-defined and stable 3-dimensional fold. Some IDPs can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. A novel outer membrane lipoprotein, bacterial interleukin r...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2016.1216294

    authors: Ahlstrand T,Tuominen H,Beklen A,Torittu A,Oscarsson J,Sormunen R,Pöllänen MT,Permi P,Ihalin R

    更新日期:2017-02-17 00:00:00

  • Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance.

    abstract::The aim of this study was to investigate if the alternative in vivo model Galleria mellonella can be used (i) to determine differences in pathogenicity of amphotericin B (AMB) resistant and susceptible A. terreus isolates, (ii) to evaluate AMB efficacy in vivo (iii) and to correlate outcome to in vitro susceptibility ...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2015.1045183

    authors: Maurer E,Browne N,Surlis C,Jukic E,Moser P,Kavanagh K,Lass-Flörl C,Binder U

    更新日期:2015-01-01 00:00:00

  • A type 6 secretion system (T6SS) encoded gene within Salmonella enterica serovar Enteritidis contributes to virulence.

    abstract::Bacteria interact with their host through protein secretion systems and surface structures. Pathogenic bacteria encode protein secretion systems that promote the invasion of the host's tissue, the evasion of the host's immune response, the thwarting microbial competitors, and ultimately survival within the host. For m...

    journal_title:Virulence

    pub_type: 评论,社论

    doi:10.1080/21505594.2017.1421829

    authors: Troxell B

    更新日期:2018-01-01 00:00:00

  • Neutrophil extracellular traps (NETs)-mediated killing of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) are impaired in patients with diabetes mellitus.

    abstract::Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) have been reported in recent years across Asian countries and pose a serious threat to public health. Neutrophils represent the first line of defense against numerous infectious pathogens, such as CR-hvKP. Neutrophil extracellular traps (NETs) constitu...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2020.1809325

    authors: Jin L,Liu Y,Jing C,Wang R,Wang Q,Wang H

    更新日期:2020-12-01 00:00:00

  • The role of inflammasome modulation in virulence.

    abstract::Pathogens frequently exist in an immunological balancing act with their host. Pathogens must not only replicate within a host but also transmit effectively between hosts to perpetuate their species. On the other hand, the host seeks to maintain homeostasis by clearing pathogens. The inflammasome is a multi-protein com...

    journal_title:Virulence

    pub_type: 杂志文章,评审

    doi:10.4161/viru.20266

    authors: Lupfer CR,Kanneganti TD

    更新日期:2012-05-01 00:00:00

  • Granulomatous-like immune reaction and hepatic fibrosis induced by Schistosoma haematobium immature worms.

    abstract::Golden hamsters were inoculated with Schistosoma haematobium cercariae to examine histological lesions at different time points over an 18 month period of infection. Hamsters were sacrificed 26 weeks and 82 weeks after inoculation. The parasite was found in the blood and in the liver of infected animals as was expecte...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.4161/viru.1.3.11348

    authors: Botelho MC,Oliveira PA,Vieira P,Delgado Mde L,Lourenço L,Lopes C,Machado JC,da Costa JM

    更新日期:2010-05-01 00:00:00

  • A D-Alanine auxotrophic live vaccine is effective against lethal infection caused by Staphylococcus aureus.

    abstract::Staphylococcus aureus infections are becoming a major global health issue due to the rapid emergence of multidrug-resistant strains. Therefore, there is an urgent need to develop an effective vaccine to prevent and control these infections. In order to develop a universal immunization strategy, we constructed a mutant...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2017.1417723

    authors: Moscoso M,García P,Cabral MP,Rumbo C,Bou G

    更新日期:2018-01-01 00:00:00

  • Typing of Streptococcus pyogenes strains using the phage profiling method.

    abstract::We recently developed a method that allows fast differentiation between Streptococcus pyogenes (GAS) strains. The method named phage profiling (PP) is based on a simple assumption that a regular PCR reaction with Taq polymerase and relatively short elongation time is not able to yield long DNA fragment, such as ~40-50...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.4161/viru.21887

    authors: Borek AL,Obszańska K,Hryniewicz W,Sitkiewicz I

    更新日期:2012-10-01 00:00:00

  • Current opinions on autophagy in pathogenicity of fungi.

    abstract::The interaction between pathogens and their host plants is a ubiquitous process. Some plant fungal pathogens can form a specific infection structure, such as an appressorium, which is formed by the accumulation of a large amount of glycerin and thereby the creation of an extremely high intracellular turgor pressure, w...

    journal_title:Virulence

    pub_type: 杂志文章,评审

    doi:10.1080/21505594.2018.1551011

    authors: Zhu XM,Li L,Wu M,Liang S,Shi HB,Liu XH,Lin FC

    更新日期:2019-12-01 00:00:00

  • Targeting Candida albicans filamentation for antifungal drug development.

    abstract::Candida albicans remains the main etiological agent of candidiasis, as this otherwise normal commensal of humans is capable of causing active infection in immune- and medically-compromised patients. The high morbidity and mortality rates associated with candidiasis, coupled with the emergence of drug resistance demand...

    journal_title:Virulence

    pub_type: 杂志文章,评审

    doi:10.1080/21505594.2016.1197444

    authors: Vila T,Romo JA,Pierce CG,McHardy SF,Saville SP,Lopez-Ribot JL

    更新日期:2017-02-17 00:00:00

  • Listeria monocytogenes encodes a functional ESX-1 secretion system whose expression is detrimental to in vivo infection.

    abstract::Bacterial pathogenicity deeply depends on the ability to secrete virulence factors that bind specific targets on host cells and manipulate host responses. The Gram-positive bacterium Listeria monocytogenes is a human foodborne pathogen that remains a serious public health concern. To transport proteins across its cell...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2016.1244589

    authors: Pinheiro J,Reis O,Vieira A,Moura IM,Zanolli Moreno L,Carvalho F,Pucciarelli MG,García-Del Portillo F,Sousa S,Cabanes D

    更新日期:2017-08-18 00:00:00

  • Passive therapy with humanized anti-staphylococcal enterotoxin B antibodies attenuates systemic inflammatory response and protects from lethal pneumonia caused by staphylococcal enterotoxin B-producing Staphylococcus aureus.

    abstract::Drugs such as linezolid that inhibit bacterial protein synthesis may be beneficial in treating infections caused by toxigenic Staphylococcus aureus. As protein synthesis inhibitors have no effect on preformed toxins, neutralization of pathogenic exotoxins with anti-toxin antibodies may be beneficial in conjunction wit...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2016.1267894

    authors: Karau MJ,Tilahun ME,Krogman A,Osborne BA,Goldsby RA,David CS,Mandrekar JN,Patel R,Rajagopalan G

    更新日期:2017-10-03 00:00:00

  • Pharmacodynamic and pharmacokinetic considerations in the treatment of critically Ill patients infected with carbapenem-resistant Enterobacteriaceae.

    abstract::Carbapenem-Resistant Enterobacteriaceae (CRE) are an emerging healthcare crisis. Infections due to CRE are associated with high morbidity and mortality, especially in critically ill patients. Due to the multi-drug resistant nature of these infections only limited treatment options are available. Antimicrobials that ha...

    journal_title:Virulence

    pub_type: 杂志文章,评审

    doi:10.1080/21505594.2016.1221021

    authors: Neuner EA,Gallagher JC

    更新日期:2017-05-19 00:00:00

  • Modeling epidemics on adaptively evolving networks: A data-mining perspective.

    abstract::The exploration of epidemic dynamics on dynamically evolving ("adaptive") networks poses nontrivial challenges to the modeler, such as the determination of a small number of informative statistics of the detailed network state (that is, a few "good observables") that usefully summarize the overall (macroscopic, system...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2015.1121357

    authors: Kattis AA,Holiday A,Stoica AA,Kevrekidis IG

    更新日期:2016-01-01 00:00:00

  • Immunomodulatory properties of Brucella melitensis lipopolysaccharide determinants on mouse dendritic cells in vitro and in vivo.

    abstract::The lipopolysaccharide (LPS) is a major virulence factor of Brucella, a facultative intracellular pathogenic Gram-negative bacterium. Brucella LPS exhibits a low toxicity and its atypical structure was postulated to delay the host immune response, favouring the establishment of chronic disease. Here we carried out an ...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2017.1386831

    authors: Zhao Y,Hanniffy S,Arce-Gorvel V,Conde-Alvarez R,Oh S,Moriyón I,Mémet S,Gorvel JP

    更新日期:2018-01-01 00:00:00

  • Formyl peptide receptor 2 orchestrates mucosal protection against Citrobacter rodentium infection.

    abstract::Citrobacter rodentium is an attaching and effacing intestinal murine pathogen which shares similar virulence strategies with the human pathogens enteropathogenic- and enterohemorrhagic Escherichia coli to infect their host. C. rodentium is spontaneously cleared by healthy wild-type (WT) mice whereas mice lacking Muc2 ...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2019.1635417

    authors: Sharba S,Venkatakrishnan V,Padra M,Winther M,Gabl M,Sundqvist M,Wang J,Forsman H,Linden SK

    更新日期:2019-12-01 00:00:00

  • Surface display of Gaussia princeps luciferase allows sensitive fungal pathogen detection during cutaneous aspergillosis.

    abstract::Non-invasive imaging techniques in microbial disease models have delivered valuable insights in the intimate pathogen-host interplay during infection. Here we describe evaluation and validation of a transgenic bioluminescence reporter strain of the human-pathogenic mold Aspergillus fumigatus, one of the main fungal pa...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.4161/viru.3.1.18799

    authors: Donat S,Hasenberg M,Schäfer T,Ohlsen K,Gunzer M,Einsele H,Löffler J,Beilhack A,Krappmann S

    更新日期:2012-01-01 00:00:00

  • The Tat system and its dependent cell division proteins are critical for virulence of extra-intestinal pathogenic Escherichia coli.

    abstract::The twin-arginine translocation (Tat) system is involved in a variety of important bacterial physiological processes. Conserved among bacteria and crucial for virulence, the Tat system is deemed as a promising anti-microbial drug target. However, the mechanism of how the Tat system functions in bacterial pathogenesis ...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2020.1817709

    authors: Liu J,Yin F,Liu T,Li S,Tan C,Li L,Zhou R,Huang Q

    更新日期:2020-12-01 00:00:00

  • Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing.

    abstract::Galleria mellonella (greater wax moth or honeycomb moth) has been introduced as an alternative model to study microbial infections. G. mellonella larvae can be easily and inexpensively obtained in large numbers and are simple to use as they don't require special lab equipment. There are no ethical constraints and thei...

    journal_title:Virulence

    pub_type: 杂志文章,评审

    doi:10.1080/21505594.2015.1135289

    authors: Tsai CJ,Loh JM,Proft T

    更新日期:2016-04-02 00:00:00

  • Identification of the pXO1 plasmid in attenuated Bacillus anthracis vaccine strains.

    abstract::Anthrax toxins and capsule are the major virulence factors of Bacillus anthracis. They are encoded by genes located on the plasmids pXO1 and pXO2, respectively. The vaccine strain Pasteur II was produced from high temperature subcultures of B. anthracis, which resulted in virulence attenuation through the loss of the ...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2016.1164366

    authors: Liang X,Zhang H,Zhang E,Wei J,Li W,Wang B,Dong S,Zhu J

    更新日期:2016-07-03 00:00:00

  • The mutated staphylococcal H35A α-toxin inhibits adhesion and invasion of Staphylococcus aureus and group A streptococci.

    abstract::In previous studies we demonstrated that the staphylococcal α-toxin inhibits adhesion and invasion of S. aureus by epithelial cells through binding to α5β1 integrin, a receptor of fibronectin. Moreover, we revealed that a H35A mutation abolishes the cytotoxicity of α-toxin completely. These findings led us to hypothes...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.4161/viru.22719

    authors: Yang J,Liang X,Ji Y

    更新日期:2013-01-01 00:00:00

  • Approved and novel strategies in diagnostics of rat bite fever and other Streptobacillus infections in humans and animals.

    abstract::Rat bite fever (RBF), a worldwide occurring and most likely under-diagnosed zoonosis caused by Streptobacillus moniliformis, represents the most prominent disease of Streptobacillus infections. Recently, novel members have been described, from which a reservoir in rats and other animal species and a zoonotic potential...

    journal_title:Virulence

    pub_type: 杂志文章,评审

    doi:10.1080/21505594.2016.1177694

    authors: Eisenberg T,Ewers C,Rau J,Akimkin V,Nicklas W

    更新日期:2016-08-17 00:00:00

  • A DUF4148 family protein produced inside RAW264.7 cells is a critical Burkholderia pseudomallei virulence factor.

    abstract::Burkholderia pseudomallei: is the etiological agent of the disease melioidosis and is a Tier 1 select agent. It survives and replicates inside phagocytic cells by escaping from the endocytic vacuole, replicating in the cytosol, spreading to other cells via actin polymerization and promoting the fusion of infected and ...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2020.1806675

    authors: Welkos S,Blanco I,Okaro U,Chua J,DeShazer D

    更新日期:2020-12-01 00:00:00

  • The ER stress response and host temperature adaptation in the human fungal pathogen Cryptococcus neoformans.

    abstract::In all eukaryotic cells, the ER stress response is pivotal to survival and adaptation under stress conditions. During temperature adaptation in the human fungal pathogen Cryptococcus neoformans, ER stress is engaged transiently. Studies of this response have demonstrated that both the engagement (turning on the respon...

    journal_title:Virulence

    pub_type: 杂志文章,评审

    doi:10.4161/viru.27187

    authors: Glazier VE,Panepinto JC

    更新日期:2014-02-15 00:00:00

  • Structural features and mechanism of translocation of non-LTR retrotransposons in Candida albicans.

    abstract::A number of abundant mobile genetic elements called retrotransposons reverse transcribe RNA to generate DNA for insertion into eukaryotic genomes. Non-long-terminal repeat (non-LTR) retrotransposons represent a major class of retrotransposons, and transposons that move by target-primed reverse transcription lack LTRs ...

    journal_title:Virulence

    pub_type: 杂志文章,评审

    doi:10.4161/viru.27278

    authors: Jiang J,Zhao L,Yan L,Zhang L,Cao Y,Wang Y,Jiang Y,Yan T,Cao Y

    更新日期:2014-02-15 00:00:00

  • Impact of daptomycin resistance on Staphylococcus aureus virulence.

    abstract::Daptomycin resistance (DAP(R)) in Staphylococcus aureus is associated with mutations in genes that are also implicated in staphylococcal pathogenesis. Using a laboratory-derived series of DAP exposed strains, we showed a relationship between increasing DAP MIC and reduced virulence in a Galleria mellonella infection m...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.1080/21505594.2015.1011532

    authors: Cameron DR,Mortin LI,Rubio A,Mylonakis E,Moellering RC Jr,Eliopoulos GM,Peleg AY

    更新日期:2015-01-01 00:00:00

  • In silico and in vitro evaluation of PCR-based assays for the detection of Bacillus anthracis chromosomal signature sequences.

    abstract::Bacillus anthracis, the causative agent of anthrax, is a zoonotic pathogen that is relatively common throughout the world and may cause life threatening diseases in animals and humans. There are many PCR-based assays in use for the detection of B. anthracis. While most of the developed assays rely on unique markers pr...

    journal_title:Virulence

    pub_type: 杂志文章

    doi:10.4161/viru.26288

    authors: Ågren J,Hamidjaja RA,Hansen T,Ruuls R,Thierry S,Vigre H,Janse I,Sundström A,Segerman B,Koene M,Löfström C,Van Rotterdam B,Derzelle S

    更新日期:2013-11-15 00:00:00