Abstract:
:Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.
journal_name
Virulencejournal_title
Virulenceauthors
Labrador-Herrera G,Pérez-Pulido AJ,Álvarez-Marín R,Casimiro-Soriguer CS,Cebrero-Cangueiro T,Morán-Barrio J,Pachón J,Viale AM,Pachón-Ibáñez MEdoi
10.1080/21505594.2020.1855912subject
Has Abstractpub_date
2020-12-01 00:00:00pages
1727-1737issue
1eissn
2150-5594issn
2150-5608journal_volume
11pub_type
杂志文章相关文献
Virulence文献大全abstract::Bloodstream infections (BSI) carry a heavy burden of morbidity and mortality in modern internal medicine wards (IMW). These wards are often filled with elderly subjects with several risk factors for BSI, such as multiple comorbidities, polypharmacy, immunosuppression, and indwelling devices. Diagnosing BSI in such a s...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2016.1140296
更新日期:2016-04-02 00:00:00
abstract::The emergence of resistance requires alternative methods to treat Candida albicans infections. We evaluated efficacy of the efflux pump inhibitor (EPI) verapamil (VER) with fluconazole (FLC) against FLC-resistant (CaR) and -susceptible C. albicans (CaS). The susceptibility of both strains to VER and FLC was determined...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1868814
更新日期:2021-12-01 00:00:00
abstract::Mycoplasmas persist in the host for a long time, suggesting that they possess mechanisms for immune evasion. Factor H is a negative regulator of the complement system, which binds to host cells to avoid unexpected complement activation. In this study, we revealed that many mycoplasmas, such as Mycoplasma hyopneumoniae...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1806664
更新日期:2020-12-01 00:00:00
abstract::Viral infections of the respiratory tract can be complicated by bacterial superinfection, resulting in a significantly longer duration of illness and even a fatal outcome. In this review, we focused on interactions between S. aureus and non-influenza viruses. Clinical data evidenced that rhinovirus infection may incre...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2018.1504561
更新日期:2018-01-01 00:00:00
abstract::The virulence behaviors of many Gram-negative bacterial pathogens are governed by quorum-sensing (QS), a hierarchical system of gene regulation that relies on population density by producing and detecting extracellular signaling molecules. Although extensively studied under in vitro conditions, adaptation of QS system...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1809327
更新日期:2020-12-01 00:00:00
abstract::We describe a young woman with profound anemia whose serum iron studies were incongruous with what we expected from iron deficiency anemia. Her high serum iron was not fully explainable until we examined the patient and noticed a large black tattoo on her left flank area. Apparently iron oxide in the ink used for the ...
journal_title:Virulence
pub_type: 信件
doi:10.4161/viru.22122
更新日期:2012-11-15 00:00:00
abstract::Intrinsically disordered proteins (IDPs) do not have a well-defined and stable 3-dimensional fold. Some IDPs can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. A novel outer membrane lipoprotein, bacterial interleukin r...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1216294
更新日期:2017-02-17 00:00:00
abstract::Candida albicans remains the main etiological agent of candidiasis, as this otherwise normal commensal of humans is capable of causing active infection in immune- and medically-compromised patients. The high morbidity and mortality rates associated with candidiasis, coupled with the emergence of drug resistance demand...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2016.1197444
更新日期:2017-02-17 00:00:00
abstract::Current treatment for combatting Chagas disease, a life-threatening illness caused by the kinetoplastid protozoan parasite Trypanosoma cruzi is inadequate, and thus the discovery of new antiparasitic compounds is of prime importance. Previous studies identified the indirubins, a class of ATP kinase inhibitors, as pote...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2018.1532242
更新日期:2018-01-01 00:00:00
abstract::Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma. H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes. The aim of the presen...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2018.1532243
更新日期:2018-01-01 00:00:00
abstract::Golden hamsters were inoculated with Schistosoma haematobium cercariae to examine histological lesions at different time points over an 18 month period of infection. Hamsters were sacrificed 26 weeks and 82 weeks after inoculation. The parasite was found in the blood and in the liver of infected animals as was expecte...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.1.3.11348
更新日期:2010-05-01 00:00:00
abstract::Non-invasive imaging techniques in microbial disease models have delivered valuable insights in the intimate pathogen-host interplay during infection. Here we describe evaluation and validation of a transgenic bioluminescence reporter strain of the human-pathogenic mold Aspergillus fumigatus, one of the main fungal pa...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.3.1.18799
更新日期:2012-01-01 00:00:00
abstract::Human herpesviruses have latency and lytic replication phases in their lifecycle. Proper regulation of herpesviral lifecycle is essential for the evasion of host immune surveillance and development of their related diseases. Recent advancements indicate a role of a novel class of viral non-coding RNAs, microRNA (miRNA...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.1.5.12966
更新日期:2010-09-01 00:00:00
abstract::Quorum-sensing (QS), the regulation of bacterial gene expression in response to changes in cell density, involves pathways that synthesize signaling molecules (auto-inducers). The luxS/AI-2-mediated QS system has been identified in both gram-positive and gram-negative bacteria. Bacillus anthracis, the etiological agen...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.1.2.10752
更新日期:2010-03-01 00:00:00
abstract::Neurodegenerative human CJD and sheep scrapie are diseases caused by several different transmissible encephalopathy (TSE) agents. These infectious agents provoke innate immune responses in the brain, including late-onset abnormal prion protein (PrP-res) amyloid. Agent particles that lack detectable PrP sequences by de...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1098804
更新日期:2015-01-01 00:00:00
abstract::In immune-competent hosts, adenoviruses (Ads) are mild pathogens that cause mainly infections of the respiratory and ocular tracks. The advent of Ad-based gene transfer vectors made the understanding of their interaction with the host cellular machinery an intensive field of research over the last decade. As studies f...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.1.3.11379
更新日期:2010-05-01 00:00:00
abstract::The interaction between pathogens and their host plants is a ubiquitous process. Some plant fungal pathogens can form a specific infection structure, such as an appressorium, which is formed by the accumulation of a large amount of glycerin and thereby the creation of an extremely high intracellular turgor pressure, w...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2018.1551011
更新日期:2019-12-01 00:00:00
abstract::Novel coronavirus disease 2019 (COVID-19), caused by novel coronavirus SARS-CoV-2, has spread globally since the end of 2019. Asymptomatic carriers are of great concern as they can undermine the interventions to stop the pandemic. However, there is limited information about the characteristics and outcomes of the asym...
journal_title:Virulence
pub_type: 杂志文章,多中心研究
doi:10.1080/21505594.2020.1802194
更新日期:2020-12-01 00:00:00
abstract::We recently developed a method that allows fast differentiation between Streptococcus pyogenes (GAS) strains. The method named phage profiling (PP) is based on a simple assumption that a regular PCR reaction with Taq polymerase and relatively short elongation time is not able to yield long DNA fragment, such as ~40-50...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.21887
更新日期:2012-10-01 00:00:00
abstract::Development of new therapeutics against Select Agents such as Francisella is critical preparation in the event of bioterrorism. Testing FDA-approved drugs for this purpose may yield new activities unrelated to their intended purpose and may hasten the discovery of new therapeutics. A library of 420 FDA-approved drugs ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1046029
更新日期:2015-01-01 00:00:00
abstract::Drugs such as linezolid that inhibit bacterial protein synthesis may be beneficial in treating infections caused by toxigenic Staphylococcus aureus. As protein synthesis inhibitors have no effect on preformed toxins, neutralization of pathogenic exotoxins with anti-toxin antibodies may be beneficial in conjunction wit...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1267894
更新日期:2017-10-03 00:00:00
abstract::The interaction between the fungal pathogen Paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. One molecule implicated in the P. brasiliensis-host interaction is the 14-3-3 pr...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1122166
更新日期:2016-01-01 00:00:00
abstract::Periodontitis is an infectious inflammatory disease that destroys the tooth-supporting tissues. It is caused by the formation of subgingival biofilms on the surface of the tooth. Characteristic bacteria associated with subgingival biofilms are the Gram-negative anaerobes Porphyromonas gingivalis, Tannerella forsythia ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2015.1081731
更新日期:2015-01-01 00:00:00
abstract::The twin-arginine translocation (Tat) system is involved in a variety of important bacterial physiological processes. Conserved among bacteria and crucial for virulence, the Tat system is deemed as a promising anti-microbial drug target. However, the mechanism of how the Tat system functions in bacterial pathogenesis ...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2020.1817709
更新日期:2020-12-01 00:00:00
abstract::The emergence of multiresistant Gram-negative bacteria requires new therapies for combating bacterial infections. Targeting the biogenesis of virulence factors could be an alternative strategy instead of killing bacteria with antibiotics. The outer membrane (OM) of Gram-negative bacteria acts as a physical barrier. At...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1278333
更新日期:2017-10-03 00:00:00
abstract::Highly pathogenic (HP) avian influenza viruses (AIV) evolve from low pathogenic (LP) precursors after circulation in poultry by reassortment and/or single mutations in different gene segments including that encoding NS1. The carboxyl terminal end (CTE) of NS1 exhibits deletions between amino acid 202 and 230 with stil...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1159367
更新日期:2016-07-03 00:00:00
abstract::Bacterial pathogens release a number of toxins that are able to form pores in target host cells, which can result in their destruction. Due to this property of this subgroup of toxins, they are considered virulence factors. A lesser known ability of these toxins when present at lower concentrations that are insufficie...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.4161/viru.1.5.12705
更新日期:2010-09-01 00:00:00
abstract::Bacillus anthracis, the causative agent of anthrax, is a zoonotic pathogen that is relatively common throughout the world and may cause life threatening diseases in animals and humans. There are many PCR-based assays in use for the detection of B. anthracis. While most of the developed assays rely on unique markers pr...
journal_title:Virulence
pub_type: 杂志文章
doi:10.4161/viru.26288
更新日期:2013-11-15 00:00:00
abstract::Bacterial pathogenicity deeply depends on the ability to secrete virulence factors that bind specific targets on host cells and manipulate host responses. The Gram-positive bacterium Listeria monocytogenes is a human foodborne pathogen that remains a serious public health concern. To transport proteins across its cell...
journal_title:Virulence
pub_type: 杂志文章
doi:10.1080/21505594.2016.1244589
更新日期:2017-08-18 00:00:00
abstract::Many of the alterations that affect innate and adaptive immune cell compartments in HIV-infected patients are reminiscent of the process of immune aging, characteristic of old age. These alterations define the immunological age of individuals and are likely to participate to the decline of immune competence with HIV d...
journal_title:Virulence
pub_type: 杂志文章,评审
doi:10.1080/21505594.2016.1195536
更新日期:2017-07-04 00:00:00