当前位置： SCI文献检索 > Nature Microbiology期刊下所有文献 > Frequency of antibiotic application drives rapid evolutionary adaptation of Escherichia coli persistence.

Frequency of antibiotic application drives rapid evolutionary adaptation of Escherichia coli persistence.

Abstract：

:The evolution of antibiotic resistance is a major threat to society and has been predicted to lead to 10 million casualties annually by 2050(1). Further aggravating the problem, multidrug tolerance in bacteria not only relies on the build-up of resistance mutations, but also on some cells epigenetically switching to a non-growing antibiotic-tolerant 'persister' state(2-6). Yet, despite its importance, we know little of how persistence evolves in the face of antibiotic treatment(7). Our evolution experiments in Escherichia coli demonstrate that extremely high levels of multidrug tolerance (20-100%) are achieved by single point mutations in one of several genes and readily emerge under conditions approximating clinical, once-daily dosing schemes. In contrast, reversion to low persistence in the absence of antibiotic treatment is relatively slow and only partially effective. Moreover, and in support of previous mathematical models(8-10), we show that bacterial persistence quickly adapts to drug treatment frequency and that the observed rates of switching to the persister state can be understood in the context of 'bet-hedging' theory. We conclude that persistence is a major component of the evolutionary response to antibiotics that urgently needs to be considered in both diagnostic testing and treatment design in the battle against multidrug tolerance.

journal_name

Nat Microbiol

journal_title

Nature microbiology

authors

Van den Bergh B,Michiels JE,Wenseleers T,Windels EM,Boer PV,Kestemont D,De Meester L,Verstrepen KJ,Verstraeten N,Fauvart M,Michiels J

doi

10.1038/nmicrobiol.2016.20

subject

Has Abstract

pub_date

2016-03-07 00:00:00

pages

16020

issn

2058-5276

pii

nmicrobiol201620

journal_volume

1

pub_type

杂志文章

相关文献

Nature Microbiology文献大全
  • Author Correction: Reactive oxygen species induce antibiotic tolerance during systemic Staphylococcus aureus infection.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature microbiology

    pub_type： 已发布勘误

    doi：10.1038/s41564-020-0679-z

    authors： Rowe SE,Wagner NJ,Li L,Beam JE,Wilkinson AD,Radlinski LC,Zhang Q,Miao EA,Conlon BP

    更新日期：2020-03-01 00:00:00

  • Environmental drivers of a microbial genomic transition zone in the ocean's interior.

    abstract：:The core properties of microbial genomes, including GC content and genome size, are known to vary widely among different bacteria and archaea 1,2 . Several hypotheses have been proposed to explain this genomic variability, but the fundamental drivers that shape bacterial and archaeal genomic properties remain uncertai...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-017-0008-3

    authors： Mende DR,Bryant JA,Aylward FO,Eppley JM,Nielsen T,Karl DM,DeLong EF

    更新日期：2017-10-01 00:00:00

  • Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer.

    abstract：:CRISPR-Cas adaptive immune systems protect bacteria and archaea against their invading genetic parasites, including bacteriophages/viruses and plasmids. In response to this immunity, many phages have anti-CRISPR (Acr) proteins that inhibit CRISPR-Cas targeting. To date, anti-CRISPR genes have primarily been discovered...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-020-0692-2

    authors： Mahendra C,Christie KA,Osuna BA,Pinilla-Redondo R,Kleinstiver BP,Bondy-Denomy J

    更新日期：2020-04-01 00:00:00

  • Sensitive high-throughput single-cell RNA-seq reveals within-clonal transcript correlations in yeast populations.

    abstract：:Single-cell RNA sequencing has revealed extensive cellular heterogeneity within many organisms, but few methods have been developed for microbial clonal populations. The yeast genome displays unusually dense transcript spacing, with interleaved and overlapping transcription from both strands, resulting in a minuscule ...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0346-9

    authors： Nadal-Ribelles M,Islam S,Wei W,Latorre P,Nguyen M,de Nadal E,Posas F,Steinmetz LM

    更新日期：2019-04-01 00:00:00

  • Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB.

    abstract：:The IncC family of broad-host-range plasmids enables the spread of antibiotic resistance genes among human enteric pathogens1-3. Although aspects of IncC plasmid conjugation have been well studied4-9, many roles of conjugation genes have been assigned based solely on sequence similarity. We applied hypersaturated tran...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-020-0775-0

    authors： Hancock SJ,Phan MD,Luo Z,Lo AW,Peters KM,Nhu NTK,Forde BM,Whitfield J,Yang J,Strugnell RA,Paterson DL,Walsh TR,Kobe B,Beatson SA,Schembri MA

    更新日期：2020-11-01 00:00:00

  • Publisher Correction: Enterotypes in the landscape of gut microbial community composition.

    abstract：:In the version of this Perspective originally published, the first and last name of co-author Manimozhiyan Arumugam were switched. This has now been corrected in all versions of the Perspective. ...

    journal_title：Nature microbiology

    pub_type： 已发布勘误

    doi：10.1038/s41564-018-0114-x

    authors： Costea PI,Hildebrand F,Arumugam M,Bäckhed F,Blaser MJ,Bushman FD,de Vos WM,Ehrlich SD,Fraser CM,Hattori M,Huttenhower C,Jeffery IB,Knights D,Lewis JD,Ley RE,Ochman H,O'Toole PW,Quince C,Relman DA,Shanahan F,Sunaga

    更新日期：2018-03-01 00:00:00

  • Dimethylsulfoniopropionate biosynthesis in marine bacteria and identification of the key gene in this process.

    abstract：:Dimethylsulfoniopropionate (DMSP) is one of the Earth's most abundant organosulfur molecules, a signalling molecule1, a key nutrient for marine microorganisms2,3 and the major precursor for gaseous dimethyl sulfide (DMS). DMS, another infochemical in signalling pathways4, is important in global sulfur cycling2 and aff...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2017.9

    authors： Curson AR,Liu J,Bermejo Martínez A,Green RT,Chan Y,Carrión O,Williams BT,Zhang SH,Yang GP,Bulman Page PC,Zhang XH,Todd JD

    更新日期：2017-02-13 00:00:00

  • Pneumococcal quorum sensing drives an asymmetric owner-intruder competitive strategy during carriage via the competence regulon.

    abstract：:Competition among microorganisms is a key determinant of successful host colonization and persistence. For Streptococcus pneumoniae, lower than predicted rates of co-colonizing strains suggest a competitive advantage for resident bacteria over newcomers. In light of evolutionary theory, we hypothesized that S. pneumon...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0314-4

    authors： Shen P,Lees JA,Bee GCW,Brown SP,Weiser JN

    更新日期：2019-01-01 00:00:00

  • Genome reduction in an abundant and ubiquitous soil bacterium 'Candidatus Udaeobacter copiosus'.

    abstract：:Although bacteria within the Verrucomicrobia phylum are pervasive in soils around the world, they are under-represented in both isolate collections and genomic databases. Here, we describe a single verrucomicrobial group within the class Spartobacteria that is not closely related to any previously described taxa. We e...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.198

    authors： Brewer TE,Handley KM,Carini P,Gilbert JA,Fierer N

    更新日期：2016-10-31 00:00:00

  • Clostridium difficile toxins induce VEGF-A and vascular permeability to promote disease pathogenesis.

    abstract：:Clostridium difficile infection (CDI) is mediated by two major exotoxins, toxin A (TcdA) and toxin B (TcdB), that damage the colonic epithelial barrier and induce inflammatory responses. The function of the colonic vascular barrier during CDI has been relatively understudied. Here we report increased colonic vascular ...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0300-x

    authors： Huang J,Kelly CP,Bakirtzi K,Villafuerte Gálvez JA,Lyras D,Mileto SJ,Larcombe S,Xu H,Yang X,Shields KS,Zhu W,Zhang Y,Goldsmith JD,Patel IJ,Hansen J,Huang M,Yla-Herttuala S,Moss AC,Paredes-Sabja D,Pothoulakis C,Shah

    更新日期：2019-02-01 00:00:00

  • A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses.

    abstract：:Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpr...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0433-6

    authors： Takada K,Kawakami C,Fan S,Chiba S,Zhong G,Gu C,Shimizu K,Takasaki S,Sakai-Tagawa Y,Lopes TJS,Dutta J,Khan Z,Kriti D,van Bakel H,Yamada S,Watanabe T,Imai M,Kawaoka Y

    更新日期：2019-08-01 00:00:00

  • Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis.

    abstract：:In most Gram-negative pathogens, the hydrolysis of UDP-2,3-diacylglucosamine to generate lipid X in lipid A biosynthesis is catalysed by the membrane-associated enzyme LpxH. We report the crystal structure of LpxH in complex with its product, lipid X, unveiling a unique insertion lid above the conserved architecture o...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.154

    authors： Cho J,Lee CJ,Zhao J,Young HE,Zhou P

    更新日期：2016-08-15 00:00:00

  • Lactate signalling regulates fungal β-glucan masking and immune evasion.

    abstract：:As they proliferate, fungi expose antigens at their cell surface that are potent stimulators of the innate immune response, and yet the commensal fungus Candida albicans is able to colonize immuno competent individuals. We show that C. albicans may evade immune detection by presenting a moving immunological target. We...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.238

    authors： Ballou ER,Avelar GM,Childers DS,Mackie J,Bain JM,Wagener J,Kastora SL,Panea MD,Hardison SE,Walker LA,Erwig LP,Munro CA,Gow NA,Brown GD,MacCallum DM,Brown AJ

    更新日期：2016-12-12 00:00:00

  • The genome of Onchocerca volvulus, agent of river blindness.

    abstract：:Human onchocerciasis is a serious neglected tropical disease caused by the filarial nematode Onchocerca volvulus that can lead to blindness and chronic disability. Control of the disease relies largely on mass administration of a single drug, and the development of new drugs and vaccines depends on a better knowledge ...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.216

    authors： Cotton JA,Bennuru S,Grote A,Harsha B,Tracey A,Beech R,Doyle SR,Dunn M,Hotopp JC,Holroyd N,Kikuchi T,Lambert O,Mhashilkar A,Mutowo P,Nursimulu N,Ribeiro JM,Rogers MB,Stanley E,Swapna LS,Tsai IJ,Unnasch TR,Voronin

    更新日期：2016-11-21 00:00:00

  • A surface endogalactanase in Bacteroides thetaiotaomicron confers keystone status for arabinogalactan degradation.

    abstract：:Glycans are major nutrients for the human gut microbiota (HGM). Arabinogalactan proteins (AGPs) comprise a heterogenous group of plant glycans in which a β1,3-galactan backbone and β1,6-galactan side chains are conserved. Diversity is provided by the variable nature of the sugars that decorate the galactans. The mecha...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0258-8

    authors： Cartmell A,Muñoz-Muñoz J,Briggs JA,Ndeh DA,Lowe EC,Baslé A,Terrapon N,Stott K,Heunis T,Gray J,Yu L,Dupree P,Fernandes PZ,Shah S,Williams SJ,Labourel A,Trost M,Henrissat B,Gilbert HJ

    更新日期：2018-11-01 00:00:00

  • Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli.

    abstract：:Tigecycline is one of the last-resort antibiotics to treat complicated infections caused by both multidrug-resistant Gram-negative and Gram-positive bacteria1. Tigecycline resistance has sporadically occurred in recent years, primarily due to chromosome-encoding mechanisms, such as overexpression of efflux pumps and r...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0496-4

    authors： Sun J,Chen C,Cui CY,Zhang Y,Liu X,Cui ZH,Ma XY,Feng Y,Fang LX,Lian XL,Zhang RM,Tang YZ,Zhang KX,Liu HM,Zhuang ZH,Zhou SD,Lv JN,Du H,Huang B,Yu FY,Mathema B,Kreiswirth BN,Liao XP,Chen L,Liu YH

    更新日期：2019-09-01 00:00:00

  • Bacterial alginate regulators and phage homologs repress CRISPR-Cas immunity.

    abstract：:CRISPR-Cas systems are adaptive immune systems that protect bacteria from bacteriophage (phage) infection1. To provide immunity, RNA-guided protein surveillance complexes recognize foreign nucleic acids, triggering their destruction by Cas nucleases2. While the essential requirements for immune activity are well under...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-020-0691-3

    authors： Borges AL,Castro B,Govindarajan S,Solvik T,Escalante V,Bondy-Denomy J

    更新日期：2020-05-01 00:00:00

  • Coordinated regulation of growth, activity and transcription in natural populations of the unicellular nitrogen-fixing cyanobacterium Crocosphaera.

    abstract：:The temporal dynamics of phytoplankton growth and activity have large impacts on fluxes of matter and energy, yet obtaining in situ metabolic measurements of sufficient resolution for even dominant microorganisms remains a considerable challenge. We performed Lagrangian diel sampling with synoptic measurements of popu...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2017.118

    authors： Wilson ST,Aylward FO,Ribalet F,Barone B,Casey JR,Connell PE,Eppley JM,Ferrón S,Fitzsimmons JN,Hayes CT,Romano AE,Turk-Kubo KA,Vislova A,Armbrust EV,Caron DA,Church MJ,Zehr JP,Karl DM,DeLong EF

    更新日期：2017-07-31 00:00:00

  • Dinoflagellates alter their carbon and nutrient metabolic strategies across environmental gradients in the central Pacific Ocean.

    abstract：:Marine microeukaryotes play a fundamental role in biogeochemical cycling through the transfer of energy to higher trophic levels and vertical carbon transport. Despite their global importance, microeukaryote physiology, nutrient metabolism and contributions to carbon cycling across offshore ecosystems are poorly chara...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-020-00814-7

    authors： Cohen NR,McIlvin MR,Moran DM,Held NA,Saunders JK,Hawco NJ,Brosnahan M,DiTullio GR,Lamborg C,McCrow JP,Dupont CL,Allen AE,Saito MA

    更新日期：2021-02-01 00:00:00

  • The anti-inflammatory drug mesalamine targets bacterial polyphosphate accumulation.

    abstract：:Mesalamine serves as the gold standard in treating ulcerative colitis. However, its precise mechanism(s) of action remains unclear. Here, we show that mesalamine treatment rapidly decreases polyphosphate levels in diverse bacteria, including members of the human gut microbiome. This decrease sensitizes bacteria toward...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.267

    authors： Dahl JU,Gray MJ,Bazopoulou D,Beaufay F,Lempart J,Koenigsknecht MJ,Wang Y,Baker JR,Hasler WL,Young VB,Sun D,Jakob U

    更新日期：2017-01-23 00:00:00

  • CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria.

    abstract：:It is generally believed that exchange of secondary metabolite biosynthetic gene clusters (BGCs) among closely related bacteria is an important driver of BGC evolution and diversification. Applying this idea may help researchers efficiently connect many BGCs to their products and characterize the products' roles in va...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0573-8

    authors： Wang G,Zhao Z,Ke J,Engel Y,Shi YM,Robinson D,Bingol K,Zhang Z,Bowen B,Louie K,Wang B,Evans R,Miyamoto Y,Cheng K,Kosina S,De Raad M,Silva L,Luhrs A,Lubbe A,Hoyt DW,Francavilla C,Otani H,Deutsch S,Washton NM,

    更新日期：2019-12-01 00:00:00

  • Immune priming and clearance of orally acquired RNA viruses in Drosophila.

    abstract：:Immune responses in insects are differentially triggered depending on the infection route used by the pathogen. In most studies involving Drosophila melanogaster and viruses, infection is done by injection, while oral infection, which is probably the most common route of viral entry in nature, remains unexplored. Here...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0265-9

    authors： Mondotte JA,Gausson V,Frangeul L,Blanc H,Lambrechts L,Saleh MC

    更新日期：2018-12-01 00:00:00

  • Mini viral RNAs act as innate immune agonists during influenza virus infection.

    abstract：:The molecular processes that determine the outcome of influenza virus infection in humans are multifactorial and involve a complex interplay between host, viral and bacterial factors1. However, it is generally accepted that a strong innate immune dysregulation known as 'cytokine storm' contributes to the pathology of ...

    journal_title：Nature microbiology

    pub_type： 信件

    doi：10.1038/s41564-018-0240-5

    authors： Te Velthuis AJW,Long JC,Bauer DLV,Fan RLY,Yen HL,Sharps J,Siegers JY,Killip MJ,French H,Oliva-Martín MJ,Randall RE,de Wit E,van Riel D,Poon LLM,Fodor E

    更新日期：2018-11-01 00:00:00

  • Exaggerated headline shock.

    abstract：: ...

    journal_title：Nature microbiology

    pub_type： 社论

    doi：10.1038/s41564-019-0408-7

    authors：

    更新日期：2019-03-01 00:00:00

  • Escherichia coli Rho GTPase-activating toxin CNF1 mediates NLRP3 inflammasome activation via p21-activated kinases-1/2 during bacteraemia in mice.

    abstract：:Inflammasomes are signalling platforms that are assembled in response to infection or sterile inflammation by cytosolic pattern recognition receptors. The consequent inflammasome-triggered caspase-1 activation is critical for the host defence against pathogens. During infection, NLRP3, which is a pattern recognition r...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-020-00832-5

    authors： Dufies O,Doye A,Courjon J,Torre C,Michel G,Loubatier C,Jacquel A,Chaintreuil P,Majoor A,Guinamard RR,Gallerand A,Saavedra PHV,Verhoeyen E,Rey A,Marchetti S,Ruimy R,Czerucka D,Lamkanfi M,Py BF,Munro P,Visvikis O,

    更新日期：2021-01-11 00:00:00

  • Influenza virus mRNA trafficking through host nuclear speckles.

    abstract：:Influenza A virus is a human pathogen with a genome composed of eight viral RNA segments that replicate in the nucleus. Two viral mRNAs are alternatively spliced. The unspliced M1 mRNA is translated into the matrix M1 protein, while the ion channel M2 protein is generated after alternative splicing. These proteins are...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.69

    authors： Mor A,White A,Zhang K,Thompson M,Esparza M,Muñoz-Moreno R,Koide K,Lynch KW,García-Sastre A,Fontoura BM

    更新日期：2016-05-27 00:00:00

  • Unexpected mode of engagement between enterovirus 71 and its receptor SCARB2.

    abstract：:Enterovirus 71 (EV71) is a common cause of hand, foot and mouth disease-a disease endemic especially in the Asia-Pacific region1. Scavenger receptor class B member 2 (SCARB2) is the major receptor of EV71, as well as several other enteroviruses responsible for hand, foot and mouth disease, and plays a key role in cell...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0319-z

    authors： Zhou D,Zhao Y,Kotecha A,Fry EE,Kelly JT,Wang X,Rao Z,Rowlands DJ,Ren J,Stuart DI

    更新日期：2019-03-01 00:00:00

  • A nonlinear time-series analysis approach to identify thresholds in associations between population antibiotic use and rates of resistance.

    abstract：:Balancing access to antibiotics with the control of antibiotic resistance is a global public health priority. At present, antibiotic stewardship is informed by a 'use it and lose it' principle, in which antibiotic use by the population is linearly related to resistance rates. However, theoretical and mathematical mode...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0410-0

    authors： López-Lozano JM,Lawes T,Nebot C,Beyaert A,Bertrand X,Hocquet D,Aldeyab M,Scott M,Conlon-Bingham G,Farren D,Kardos G,Fésűs A,Rodríguez-Baño J,Retamar P,Gonzalo-Jiménez N,Gould IM,THRESHOLDS study group.

    更新日期：2019-07-01 00:00:00

  • D-Alanylation of teichoic acids contributes to Lactobacillus plantarum-mediated Drosophila growth during chronic undernutrition.

    abstract：:The microbial environment influences animal physiology. However, the underlying molecular mechanisms of such functional interactions are largely undefined. Previously, we showed that during chronic undernutrition, strains of Lactobacillus plantarum, a major commensal partner of Drosophila, promote host juvenile growth...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-017-0038-x

    authors： Matos RC,Schwarzer M,Gervais H,Courtin P,Joncour P,Gillet B,Ma D,Bulteau AL,Martino ME,Hughes S,Chapot-Chartier MP,Leulier F

    更新日期：2017-12-01 00:00:00

  • Epstein-Barr virus ncRNA from a nasopharyngeal carcinoma induces an inflammatory response that promotes virus production.

    abstract：:The Epstein-Barr virus M81 strain, isolated from a nasopharyngeal carcinoma, induces potent spontaneous virus production in infected B cells. We found that the M81 non-coding Epstein-Barr-encoded RNA EBER2, which carries polymorphisms that are mainly restricted to viruses found in endemic nasopharyngeal carcinomas, ma...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0546-y

    authors： Li Z,Tsai MH,Shumilov A,Baccianti F,Tsao SW,Poirey R,Delecluse HJ

    更新日期：2019-12-01 00:00:00