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A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses.

Abstract：

:Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.

journal_name

Nat Microbiol

journal_title

Nature microbiology

authors

Takada K,Kawakami C,Fan S,Chiba S,Zhong G,Gu C,Shimizu K,Takasaki S,Sakai-Tagawa Y,Lopes TJS,Dutta J,Khan Z,Kriti D,van Bakel H,Yamada S,Watanabe T,Imai M,Kawaoka Y

doi

10.1038/s41564-019-0433-6

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

1268-1273

issue

8

issn

2058-5276

pii

10.1038/s41564-019-0433-6

journal_volume

4

pub_type

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