Abstract:
:An important unsolved challenge in tissue engineering has been the inability to replicate the geometry and function of vascular networks and blood vessels. Here, we engineer a user-defined 3D microfluidic vascular channel using 3D printing-enabled hydrogel casting. First, a hollow L-shaped channel is developed using a template casting process. In this process, murine 10T1/2 cells are encapsulated within gelatin methacrylate (GelMA) hydrogel using UV photocrosslinking, and upon removal of the template results in a hollow channel within GelMA. Second, human umbilical vein endothelial cells (HUVECs) were cultured within the channel and immunostaining was used to visualize endothelial monolayers. Third, diffusion/permeability studies on endothelialized channels were carried out to demonstrate the barrier function of HUVEC monolayer. Taken together, we develop a facile, cytocompatible and rapid approach to engineer a user-defined multicellular vascular chip that could be potentially useful in developing new vascular model systems.
journal_name
Biofabricationjournal_title
Biofabricationauthors
Yang L,Shridhar SV,Gerwitz M,Soman Pdoi
10.1088/1758-5090/8/3/035015subject
Has Abstractpub_date
2016-08-26 00:00:00pages
035015issue
3eissn
1758-5082issn
1758-5090journal_volume
8pub_type
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