Integrating self-assembly and biofabrication for the development of structures with enhanced complexity and hierarchical control.

abstract：:Bioprinting of living cells is rapidly developing as an advanced biofabrication approach to engineer tissues. Bioinks can be extruded in three-dimensions (3D) to fabricate complex and hierarchical constructs for implantation. However, a lack of functionality can often be attributed to poor bioink properties. Indeed, a...

journal_title：Biofabrication

authors： Cidonio G,Alcala-Orozco CR,Lim KS,Glinka M,Mutreja I,Kim YH,Dawson JI,Woodfield TBF,Oreffo ROC

更新日期：2019-06-12 00:00:00

abstract：:We report on the fabrication of silicon-reinforced carbon (C:Si) structures by combinatorial pulsed laser deposition to search for the best design for a new generation of multi-functional coated implants. The synthesized films were characterized from the morphological, structural, compositional, mechanical and microbi...

journal_title：Biofabrication

authors： Mihailescu IN,Bociaga D,Popescu-Pelin G,Stan GE,Duta L,Socol G,Chifiriuc MC,Bleotu C,Lazar V,Husanu MA,Zgura I,Miculescu F,Negut I,Hapenciuc C

更新日期：2017-06-01 00:00:00

abstract：:Cell cultures for tissue engineering are traditionally prepared on two-dimensional or three-dimensional scaffolds with simple pores; however, this limits mass transportation, which is necessary for cell viability and function. In this paper, an innovative method is proposed for fabricating porous scaffolds with design...

journal_title：Biofabrication

authors： Liu Y,Li X,Qu X,Zhu L,He J,Zhao Q,Wu W,Li D

更新日期：2012-03-01 00:00:00

abstract：:Native small intestine possesses distinct multi-scale structures (e.g., crypts, villi) not included in traditional 2D intestinal culture models for drug delivery and regenerative medicine. The known impact of structure on cell function motivates exploration of the influence of intestinal topography on the phenotype of...

journal_title：Biofabrication

authors： Koppes AN,Kamath M,Pfluger CA,Burkey DD,Dokmeci M,Wang L,Carrier RL

更新日期：2016-08-22 00:00:00

abstract：:Technical limitations of traditional electrospinning make it hard to produce three-dimensional (3D) scaffolds with hierarchical pore structures. Here, porous polycaprolactone (PCL) nanofiber meshes with different nano-hydroxyapatite (nHA) concentrations were prepared by electrospinning with stainless steel mesh as the...

journal_title：Biofabrication

authors： Song J,Zhu G,Wang L,An G,Shi X,Wang Y

更新日期：2017-02-14 00:00:00

abstract：:Omentum-based matrices fabricated by decellularization have the potential to serve as autologous scaffolds for tissue engineering. Transplantation of such scaffolds prepared from the patient's own biomaterial may reduce the immunogenic response after transplantation. Recently we reported on the potential of the decell...

journal_title：Biofabrication

authors： Soffer-Tsur N,Shevach M,Shapira A,Peer D,Dvir T

更新日期：2014-09-01 00:00:00

abstract：:Three-dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive additive manufacturin...

journal_title：Biofabrication

authors： Flood P,Alvarez L,Reynaud EG

更新日期：2016-10-11 00:00:00

abstract：:How to pack materials into well-defined volumes efficiently has been a longstanding question of interest to physicists, material scientists, and mathematicians as these materials have broad applications ranging from shipping goods in commerce to seeds in agriculture and to spheroids in tissue engineering. How many mar...

journal_title：Biofabrication

authors： Ren T,Steiger W,Chen P,Ovsianikov A,Demirci U

更新日期：2020-03-31 00:00:00

abstract：:In this study, we report the step-gradient nanocomposite (NC) hydrogel generated easily by spatial connection of different nanocomposite hydrogel pastes varying in the concentrations of nanomaterials with the aid of a 3D printing technique. The prepared 3D printed gradient NC hydrogel has self-adhesive properties and ...

journal_title：Biofabrication

authors： Motealleh A,Çelebi-Saltik B,Ermis N,Nowak S,Khademhosseini A,Kehr NS

更新日期：2019-08-22 00:00:00

abstract：:This paper describes a multi-material virtual prototyping (MMVP) system for modelling and digital fabrication of discrete and functionally graded multi-material objects for biomedical applications. The MMVP system consists of a DMMVP module, an FGMVP module and a virtual reality (VR) simulation module. The DMMVP modul...

journal_title：Biofabrication

authors： Cheung HH,Choi SH

更新日期：2009-12-01 00:00:00

abstract：:As an alternative to conventional cell culture and animal testing, an organ-on-a-chip is applied to study the biological phenomena of organ development and disease, as well as the interactions between human tissues and external stimuli such as chemicals, forces and electricity. The pattern design of a microfluidic cha...

journal_title：Biofabrication

authors： Mao M,He J,Lu Y,Li X,Li T,Zhou W,Li D

更新日期：2018-02-05 00:00:00

abstract：:The physical and mechanical properties of cells modulate their behavior such proliferation rate, migration and extracellular matrix remodeling. In order to study cell behavior in a tissue-like environment in vitro, it is of utmost importance to develop biologically and physically relevant 3D cell models. Here, we char...

journal_title：Biofabrication

authors： Pillet F,Gibot L,Madi M,Rols MP,Dague E

更新日期：2017-05-11 00:00:00

abstract：:The tumour microenvironment is heterogeneous and consists of multiple cell types, variable extracellular matrix (ECM) composition, and contains cell-defined gradients of small molecules, oxygen, nutrients and waste. Emerging in vitro cell culture systems that attempt to replicate these features often fail to incorpora...

journal_title：Biofabrication

authors： Rodenhizer D,Cojocari D,Wouters BG,McGuigan AP

更新日期：2016-10-18 00:00:00

abstract：:An engineered three-dimensional scaffold with hierarchical porosity and multiple niche microenvironments is produced using a combined multi-nozzle deposition-freeze casting technique. In this paper we present a process to fabricate a scaffold with improved interconnectivity and hierarchical porosity. The scaffold is p...

journal_title：Biofabrication

authors： Snyder JE,Hunger PM,Wang C,Hamid Q,Wegst UG,Sun W

更新日期：2014-03-01 00:00:00

abstract：:To overcome the drawbacks of in vitro liver testing during drug development, numerous liver-on-a-chip models have been developed. However, current liver-on-a-chip technologies are labor-intensive, lack extracellular matrix (ECM) essential for liver cells, and lack a biliary system essential for excreting bile acids, w...

journal_title：Biofabrication

authors： Lee H,Chae S,Kim JY,Han W,Kim J,Choi Y,Cho DW

更新日期：2019-01-16 00:00:00

abstract：:Silica-bonded calcite scaffolds have been successfully 3D-printed by direct ink writing, starting from a paste comprising a silicone polymer and calcite powders, calibrated in order to match a SiO2/CaCO3 weight balance of 35/65. The scaffolds, fabricated with two slightly different geometries, were first cross-linked ...

journal_title：Biofabrication

authors： Fiocco L,Elsayed H,Badocco D,Pastore P,Bellucci D,Cannillo V,Detsch R,Boccaccini AR,Bernardo E

更新日期：2017-05-11 00:00:00

abstract：:The high attrition rate of neuro-pharmaceuticals as they proceed to market necessitates the development of clinically-relevant in vitro neural microphysiological systems that can be utilized during the preclinical screening phase to assess the safety and efficacy of potential compounds. Historically, proposed models h...

journal_title：Biofabrication

authors： Bowser DA,Moore MJ

更新日期：2019-10-21 00:00:00

abstract：:Here we developed a composite scaffold of pearl/poly(lactic-co-glycolic acid) (pearl/PLGA) utilizing the low-temperature deposition manufacturing (LDM). LDM makes it possible to fabricate scaffolds with designed microstructure and macrostructure, while keeping the bioactivity of biomaterials by working at a low temper...

journal_title：Biofabrication

authors： Xu M,Li Y,Suo H,Yan Y,Liu L,Wang Q,Ge Y,Xu Y

更新日期：2010-06-01 00:00:00

abstract：:Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation...

journal_title：Biofabrication

authors： Gruene M,Pflaum M,Deiwick A,Koch L,Schlie S,Unger C,Wilhelmi M,Haverich A,Chichkov BN

更新日期：2011-03-01 00:00:00

abstract：:We previously developed the Bio-Pick, Place, and Perfuse (Bio-P3) instrument to fabricate large perfusable tissue constructs by stacking and aligning scaffold-free living microtissues with integrated lumens. The Bio-P3 required an actuating mechanism to manipulate living microtissues of various sizes and shapes that a...

journal_title：Biofabrication

authors： Ip BC,Cui F,Tripathi A,Morgan JR

更新日期：2016-05-25 00:00:00

abstract：:Tissue rings with incorporated microscaffolds have been engineered as promising building blocks for constructing biological tubes from the bottom up. However, the microscaffolds available for incorporation are very limited at present. In this paper we provide an efficient strategy to first incorporate microfluidic spu...

journal_title：Biofabrication

authors： Sun T,Shi Q,Yao Y,Sun J,Wang H,Huang Q,Fukuda T

更新日期：2019-06-25 00:00:00

abstract：:Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated. Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers whic...

journal_title：Biofabrication

authors： Chen S,Wu C,Liu A,Wei D,Xiao Y,Guo Z,Chen L,Zhu Y,Sun J,Luo H,Fan H

更新日期：2020-05-12 00:00:00

abstract：:Degeneration of the intervertebral disc (IVD) represents a significant musculoskeletal disease burden. Tissue engineering has proposed several strategies comprising the use of biodegradable materials to prepare scaffolds that can present mechanical properties similar to those of native IVD tissues. However, this might...

journal_title：Biofabrication

authors： van Uden S,Silva-Correia J,Correlo VM,Oliveira JM,Reis RL

更新日期：2015-01-21 00:00:00

abstract：:Cell mechanical microenvironment (CMM) significantly affects cell behaviors such as spreading, migration, proliferation and differentiation. However, most studies on cell response to mechanical stimulation are based on two-dimensional (2D) planar substrates, which cannot mimic native three-dimensional (3D) CMM. Accumu...

journal_title：Biofabrication

authors： Huang G,Wang L,Wang S,Han Y,Wu J,Zhang Q,Xu F,Lu TJ

更新日期：2012-12-01 00:00:00

abstract：:Despite the widespread use as platforms for various biomedical applications, engineering hydrogels to impart multifunctionality and control physical properties, while closely mimicking the native cellular microenvironment, is still a significant challenge. Herein, nanofibers consisting of hydrophilic and photocrosslin...

journal_title：Biofabrication

authors： Kim S,Cha C

更新日期：2019-12-19 00:00:00

abstract：:Drop-on-demand (DoD) inkjet printing has been explored for a range of applications, including those to selectively deposit cellular material, due to the high accuracy and scalability of such systems when compared with alternative bioprinting techniques. Despite this, there remain considerable limitations when handling...

journal_title：Biofabrication

authors： Dudman JPR,Ferreira AM,Gentile P,Wang X,Ribeiro RDC,Benning M,Dalgarno KW

更新日期：2020-08-12 00:00:00

abstract：:A Caco-2 cell culture on Transwell, an alternative testing to animal or human testing used in evaluating drug intestinal permeability, incorrectly estimated the absorption of actively transported drugs due to the low expression of membrane transporters. Similarly, three-dimensional (3D) cultures of Caco-2 cells, which...

journal_title：Biofabrication

authors： Shen C,Meng Q,Zhang G

更新日期：2014-12-17 00:00:00

abstract：:Electrospun fibers of natural polymers are desirable for biomedical applications such as tissue engineering. Crosslinking of electrospun fibers of natural polymers is needed to prevent dissolution in water and to enhance mechanical strength. In this study, an in situ UV-crosslinking method was developed for crosslinki...

journal_title：Biofabrication

authors： Lin WH,Tsai WB

更新日期：2013-09-01 00:00:00

abstract：:Calcium alginate hydrogels are widely used as biocompatible materials in a substantial number of biomedical applications. This paper reports on a hybrid 3D printing and electrodeposition approach for forming 3D calcium alginate hydrogels in a controllable manner. Firstly, a specific 3D hydrogel printing system is deve...

journal_title：Biofabrication

authors： Shang W,Liu Y,Wan W,Hu C,Liu Z,Wong CT,Fukuda T,Shen Y

更新日期：2017-06-07 00:00:00

abstract：:In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate bl...

journal_title：Biofabrication

authors： Marchioli G,van Gurp L,van Krieken PP,Stamatialis D,Engelse M,van Blitterswijk CA,Karperien MB,de Koning E,Alblas J,Moroni L,van Apeldoorn AA

更新日期：2015-05-28 00:00:00