Extracting DNA from 'jaws': high yield and quality from archived tiger shark (Galeocerdo cuvier) skeletal material.

abstract：:Microsatellite genotyping of hair and faeces using standard polymerase chain reaction (PCR) resulted in low success rates and high error rates in a 2003-2004 pilot study using noninvasive genetic sampling for the brown bear (Ursus arctos) in the Italian Alps. Thus, we evaluated the performance of multiplex pre-amplifi...

journal_title：Molecular ecology resources

authors： DE Barba M,Waits LP

更新日期：2010-07-01 00:00:00

abstract：:Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183...

journal_title：Molecular ecology resources

authors： Cruz VP,Vera M,Pardo BG,Taggart J,Martinez P,Oliveira C,Foresti F

更新日期：2017-05-01 00:00:00

abstract：:With striking morphological diversity and adaptability, Chamaedorea palms constitute an ecologically and economically important understorey component of Neotropical forests. Nine loci developed for Chamaedorea elegans evaluated in three Veracruz populations resulted in a large number of alleles (8-18), and high expect...

journal_title：Molecular ecology resources

authors： Cibrián-Jaramillo A,Hahn WJ,Desalle R

更新日期：2008-03-01 00:00:00

abstract：:Mites (Acari) are one of the most diverse groups of life on Earth; yet, their evolutionary relationships are poorly understood. Also, the resolution of broader arachnid phylogeny has been hindered by an underrepresentation of mite diversity in phylogenomic analyses. To further our understanding of Acari evolution, we ...

journal_title：Molecular ecology resources

authors： Van Dam MH,Trautwein M,Spicer GS,Esposito L

更新日期：2019-03-01 00:00:00

abstract：:We implemented multilocus selection in a spatially-explicit, individual-based framework that enables multivariate environmental gradients to drive selection in many loci as a new module for the landscape genetics programs, CDPOP and CDMetaPOP. Our module simulates multilocus selection using a linear additive model, pr...

journal_title：Molecular ecology resources

authors： Landguth EL,Forester BR,Eckert AJ,Shirk AJ,Menon M,Whipple A,Day CC,Cushman SA

更新日期：2020-03-01 00:00:00

abstract：:The emu (Dromaius novaehollandiae), a member of ratite family, is native to Australia and has been introduced to other countries worldwide. In this work, 10 polymorphic microsatellite loci were isolated and characterized for emu from public sequences. Polymorphism was surveyed in 22 individuals from two different popu...

journal_title：Molecular ecology resources

authors： Yáñez JM,González R,Angulo J,Vidal R,Santos JL,Martínez V

更新日期：2008-11-01 00:00:00

abstract：:Single nucleotide polymorphisms (SNPs) have become the marker of choice for genetic studies in organisms of conservation, commercial or biological interest. Most SNP discovery projects in nonmodel organisms apply a strategy for identifying putative SNPs based on filtering rules that account for random sequencing error...

journal_title：Molecular ecology resources

authors： Gonçalves da Silva A,Barendse W,Kijas JW,Barris WC,McWilliam S,Bunch RJ,McCullough R,Harrison B,Hoelzel AR,England PR

更新日期：2015-07-01 00:00:00

abstract：:Next-generation sequencing is a common method for analysing microbial community diversity and composition. Configuring an appropriate sequence processing strategy within the variety of tools and methods is a nontrivial task and can considerably influence the resulting community characteristics. We analysed the V4 regi...

journal_title：Molecular ecology resources

authors： Hardge K,Neuhaus S,Kilias ES,Wolf C,Metfies K,Frickenhaus S

更新日期：2018-03-01 00:00:00

abstract：:We present 25 polymorphic microsatellite loci for Intsia palembanica, a highly valued timber species in the Indo-Malayan region, Australia and western Pacific islands. Microsatellite loci were tested for polymorphism across a total of 76 individuals from three natural populations from Irian Jaya, Indonesia. The averag...

journal_title：Molecular ecology resources

authors： Wong KN,Tan WL,Chew FT

更新日期：2009-01-01 00:00:00

abstract：:Thirteen polymorphic microsatellite markers were isolated and characterized from the great hornbill, Buceros bicornis. In analyses of 20 individuals, the numbers of alleles per locus varied from two to 11. The expected and observed heterozygosities ranged from 0.22 to 0.88 and from 0.20 to 1.00, respectively. The mean...

journal_title：Molecular ecology resources

authors： Chamutpong S,Saito DS,Viseshakul N,Nishiumi I,Poonswad P,Ponglikitmongkol M

更新日期：2009-03-01 00:00:00

abstract：:In noninvasive genetic sampling, when genotyping error rates are high and recapture rates are low, misidentification of individuals can lead to overestimation of population size. Thus, estimating genotyping errors is imperative. Nonetheless, conducting multiple polymerase chain reactions (PCRs) at multiple loci is tim...

journal_title：Molecular ecology resources

authors： Hansen H,Ben-David M,McDonald DB

更新日期：2008-03-01 00:00:00

abstract：:Linkage disequilibrium (LD, association of allelic states across loci) is poorly understood by many evolutionary biologists, but as technology for multilocus sampling improves, we ignore LD at our peril. If we sample variation at 10 loci in an organism with 20 chromosomes, we can reasonably treat them as 10 'independe...

journal_title：Molecular ecology resources

authors： Baird SJ

更新日期：2015-09-01 00:00:00

abstract：:We describe 15 polymorphic microsatellite loci from the white-ruffed manakin Corapipo altera, a common understory bird of Neotropical lowland and montane evergreen forests from eastern Honduras to northwestern Colombia. These markers were developed in order to assess population structure and genetic diversity in a fra...

journal_title：Molecular ecology resources

authors： Barnett JR,Stenzler LM,Ruiz-Gutierrez V,Bogdanowicz SM,Lovette IJ

更新日期：2008-01-01 00:00:00

abstract：:Microsatellite loci were identified from Cornus kousa'National'. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ra...

journal_title：Molecular ecology resources

authors： Wadl PA,Wang X,Scheffler BE,Rinehart TA,Trigiano RN

更新日期：2008-07-01 00:00:00

abstract：:A microsatellite-enriched genomic library was developed for the water flea Daphnia atkinsoni Baird, 1859, a dominant species of intermittent wetlands in Europe. Eight polymorphic microsatellite markers were successfully optimized. Characterization of 77 individuals from Belgium and Spain showed moderate (in the former...

journal_title：Molecular ecology resources

authors： Ortells R,VAN Houdt JK,Geldof S,DE Meester L,Mergeay J

更新日期：2009-01-01 00:00:00

abstract：:Inferring the mode and tempo of natural selection helps further our understanding of adaptation to past environmental changes. Here, we introduce McSwan, a method to detect and date past and recent natural selection events in the case of a hard sweep. The method is based on the comparison of site frequency spectra obt...

journal_title：Molecular ecology resources

authors： Tournebize R,Poncet V,Jakobsson M,Vigouroux Y,Manel S

更新日期：2019-01-01 00:00:00

abstract：:Bactrocera cucurbitae (Coquillett) is an important insect pest of melon fruit causing extensive losses of production in Asia and Pacific areas. Twelve polymorphic microsatellite loci were isolated from an enriched DNA library of this species and their amplification characteristics are described. Genetic parameters wer...

journal_title：Molecular ecology resources

authors： Wu Y,Li Z,Wu J

更新日期：2009-09-01 00:00:00

abstract：:sam is a Windows program designed to detect candidate loci for selection in whole-genome scans. It also gives valuable clues as regards the ecological factors at stake in the selection process. The method used is based on multiple univariate logistic regression models to test for association between allelic frequencie...

journal_title：Molecular ecology resources

authors： Joost S,Kalbermatten M,Bonin A

更新日期：2008-09-01 00:00:00

abstract：:We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymo...

journal_title：Molecular ecology resources

authors： Degner JF,Hether TD,Hoffman EA

更新日期：2009-03-01 00:00:00

abstract：:Interpretation of high-throughput sequence data requires an understanding of how decisions made during bioinformatic data processing can influence results. One source of bias that is often cited is PCR clones (or PCR duplicates). PCR clones are common in restriction site-associated sequencing (RAD-seq) data sets, whic...

journal_title：Molecular ecology resources

authors： Euclide PT,McKinney GJ,Bootsma M,Tarsa C,Meek MH,Larson WA

更新日期：2020-01-01 00:00:00

abstract：:We developed and tested 14 new polymorphic microsatellite loci for dreissenid mussels, including the two species that have invaded many freshwater habitats in Eurasia and North America, where they cause serious industrial fouling damage and ecological alterations. These new loci will aid our understanding of their gen...

journal_title：Molecular ecology resources

authors： Feldheim KA,Brown JE,Murphy DJ,Stepien CA

更新日期：2011-07-01 00:00:00

abstract：:Eight polymorphic microsatellites were isolated from the Greek smooth newt (Lissotriton vulgaris graecus) using a microsatellite enrichment protocol and selective hybridization with a biotinylated (AC)(11) probe. The loci showed different variation patterns in a single breeding population (32 individuals) with mean nu...

journal_title：Molecular ecology resources

authors： Sotiropoulos K,Eleftherakos K,Tsaparis D,Kasapidis P,Magoulas A,Legakis A

更新日期：2009-01-01 00:00:00

abstract：:Recent advances in high-throughput sequencing technologies have offered the possibility to generate genomewide sequence data to delineate previously unidentified genetic structure, obtain more accurate estimates of demographic parameters and to evaluate potential adaptive divergence. Here, we identified 27 556 single ...

journal_title：Molecular ecology resources

authors： Zhang BD,Xue DX,Wang J,Li YL,Liu BJ,Liu JX

更新日期：2016-05-01 00:00:00

abstract：:High-throughput identification technologies provide efficient tools for understanding the ecology and functioning of microorganisms. Yet, these methods have been only rarely used for monitoring and testing ecological hypotheses in plant pathogens and pests in spite of their immense importance in agriculture, forestry ...

journal_title：Molecular ecology resources

authors： Tedersoo L,Drenkhan R,Anslan S,Morales-Rodriguez C,Cleary M

更新日期：2019-01-01 00:00:00

abstract：:Microsatellite markers were developed to assess population structure and patterns of translocation in the introduced European common genet (Genetta genetta). Primer pairs were designed for 60 microsatellite sequences enriched for CA, GA, CATC and TAGA repeat motifs. Eleven loci that proved to be polymorphic were genot...

journal_title：Molecular ecology resources

authors： Gaubert P,Del Cerro I,Palomares F,Godoy JA

更新日期：2008-11-01 00:00:00

abstract：:We propose a new model to make use of georeferenced genetic data for inferring the location and shape of a hybrid zone. The model output includes the posterior distribution of a parameter that quantifies the width of the hybrid zone. The model proposed is implemented in the GUI and command-line versions of the Genelan...

journal_title：Molecular ecology resources

authors： Guedj B,Guillot G

更新日期：2011-11-01 00:00:00

abstract：:We report the isolation and characterization of eight microsatellite loci from the scleractinian coral, Acropora nobilis. The microsatellite loci were obtained using compound SSR primers or an enrichment protocol. All the loci were polymorphic with four to eight alleles per locus and observed heterozygosities ranging ...

journal_title：Molecular ecology resources

authors： Isomura N,Hidaka M

更新日期：2008-05-01 00:00:00

abstract：:Introgression can be an important evolutionary force but it can also lead to species extinction and as such is a crucial issue for species conservation. However, introgression is difficult to detect, morphologically as well as genetically. Hybridization with domestic cats (Felis silvestris catus) is a major concern fo...

journal_title：Molecular ecology resources

authors： Nussberger B,Greminger MP,Grossen C,Keller LF,Wandeler P

更新日期：2013-05-01 00:00:00

abstract：:DNA barcoding has become an important current scientific trend to the understanding of the world biodiversity. In the case of mega-diverse hot spots like Mexico, this technique represents an important tool for taxonomists, allowing them to concentrate in highlighted species by the barcodes instead of analyzing entire ...

journal_title：Molecular ecology resources

authors： Elías-Gutiérrez M,León-Regagnon V

更新日期：2013-11-01 00:00:00

abstract：:In the Alectoris partridges (Phasianidae), hybridization occurs occasionally as a result of the natural breakdown of isolating mechanisms but more frequently as a result of human activity. No genetic record of hybridization is known for the barbary partridge (A. barbara). This species is distributed mostly in North Af...

journal_title：Molecular ecology resources

authors： Barbanera F,Guerrini M,Bertoncini F,Cappelli F,Muzzeddu M,Dini F

更新日期：2011-01-01 00:00:00