Abstract:
:Microsatellite genotyping of hair and faeces using standard polymerase chain reaction (PCR) resulted in low success rates and high error rates in a 2003-2004 pilot study using noninvasive genetic sampling for the brown bear (Ursus arctos) in the Italian Alps. Thus, we evaluated the performance of multiplex pre-amplification for improving microsatellite genotyping results. Brown bear faecal DNA extracts of varying quality (n = 33) and hair DNA extracts of poor (n = 32) and good (n = 34) quality were used to compare standard PCR and pre-amplification. In contrast to previous studies, there was no significant difference between methods for individual locus amplification success, genotyping error and genotyping success rates for scat and hair samples. The use of pre-amplification requires an additional investment of time and resources, and our results raise questions about the universal value of pre-amplification approaches. We suggest that researchers carefully evaluate the performance of pre-amplification compared to standard PCR using field-collected samples from the study area of interest before engaging in large-scale noninvasive genetic analyses.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
DE Barba M,Waits LPdoi
10.1111/j.1755-0998.2009.02818.xsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
659-65issue
4eissn
1755-098Xissn
1755-0998journal_volume
10pub_type
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular ecology resources
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