Abstract:
:We identified microsatellite sequences of potential utility in the house sparrow (Passer domesticus) and assigned their predicted genome locations. These sequences included newly isolated house sparrow loci, which we fully characterized. Many of the newly isolated loci were polymorphic in two other species of Passeridae: Berthelot's pipit Anthus berthelotii and zebra finch Taeniopygia guttata. In total, we identified 179 microsatellite markers that were either isolated directly from, or are of known utility in, the house sparrow. Sixty-seven of these markers were designed from unique sequences that we isolated from a house sparrow genomic library. These new markers were combined with 36 house sparrow markers isolated by other studies and 76 markers isolated from other passerine species but known to be polymorphic in the house sparrow. We utilized sequence homology to assign chromosomal locations for these loci in the assembled zebra finch genome. One hundred and thirty-four loci were assigned to 25 different autosomes and eight loci to the Z chromosome. Examination of the genotypes of known-sex house sparrows for 37 of the new loci revealed a W-linked locus and an additional Z-linked locus. Locus Pdoμ2, previously reported as autosomal, was found to be Z-linked. These loci enable the creation of powerful and cost-effective house sparrow multiplex primer sets for population and parentage studies. They can be used to create a house sparrow linkage map and will aid the identification of quantitative trait loci in passerine species.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Dawson DA,Horsburgh GJ,Krupa AP,Stewart IR,Skjelseth S,Jensen H,Ball AD,Spurgin LG,Mannarelli ME,Nakagawa S,Schroeder J,Vangestel C,Hinten GN,Burke Tdoi
10.1111/j.1755-0998.2012.03115.xsubject
Has Abstractpub_date
2012-05-01 00:00:00pages
501-23issue
3eissn
1755-098Xissn
1755-0998journal_volume
12pub_type
杂志文章abstract::The emergence of third-generation sequencing (3GS; long-reads) is bringing closer the goal of chromosome-size fragments in de novo genome assemblies. This allows the exploration of new and broader questions on genome evolution for a number of nonmodel organisms. However, long-read technologies result in higher sequenc...
journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.13176
更新日期:2020-09-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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abstract::The European flounder (Platichthys flesus L.) is used in ecotoxicological studies to provide detailed information on the effects of pollution on individual fish. Data on population and evolutionary level effects are, however, limited. Here, the isolation and characterization of 28 novel species specific microsatellite...
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pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.12578
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.12330
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.13121
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.13215
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.12382
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.12078
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/j.1755-0998.2008.02296.x
更新日期:2009-01-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/j.1755-0998.2008.02219.x
更新日期:2008-09-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
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更新日期:2009-01-01 00:00:00
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journal_title:Molecular ecology resources
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更新日期:2011-03-01 00:00:00
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
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