Abstract:
:A multiplex haplotype-specific polymerase chain reaction (MHS-PCR) method was developed, which identified seven Clupeiform species living in the tropical Eastern Atlantic region: Sardinella aurita, Sardinella maderensis, Ethmalosa fimbriata, Sardina pilchardus, Engraulis encrasicholus, Pellonula leonensis and Ilisha africana. 16S rRNA fragments were amplified using a species-specific set of primers, yielding species-specific size fragments, and then separated using agarose gel electrophoresis, enabling direct visual identification of targeted species. This method provides an accurate, easy and rapid tool for identifying species within large Clupeiform samples. It is suitable for investigations on early Clupeiform stages, species and identification in fishery management in the tropical Eastern Atlantic area.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Durand JD,Diatta MA,Diop K,Trape Sdoi
10.1111/j.1755-0998.2009.02776.xsubject
Has Abstractpub_date
2010-05-01 00:00:00pages
568-72issue
3eissn
1755-098Xissn
1755-0998journal_volume
10pub_type
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