The quest for Y-chromosomal markers - methodological strategies for mammalian non-model organisms.

abstract：:As coral populations decline worldwide in the face of ongoing environmental change, documenting their distribution, diversity and conservation status is now more imperative than ever. Accurate delimitation and identification of species is a critical first step. This task, however, is not trivial as morphological varia...

journal_title：Molecular ecology resources

authors： Erickson KL,Pentico A,Quattrini AM,McFadden CS

更新日期：2021-01-01 00:00:00

abstract：:The advent of next-generation sequencing (NGS) technologies has transformed the way microsatellites are isolated for ecological and evolutionary investigations. Recent attempts to employ NGS for microsatellite discovery have used the 454, Illumina, and Ion Torrent platforms, but other methods including single-molecule...

journal_title：Molecular ecology resources

authors： Wei N,Bemmels JB,Dick CW

更新日期：2014-09-01 00:00:00

abstract：:Honeybee subspecies have been affected by human activities in Europe over the past few decades. One such example is the importation of nonlocal subspecies of bees which has had an adverse impact on the geographical repartition and subsequently on the genetic diversity of the black honeybee Apis mellifera mellifera. To...

journal_title：Molecular ecology resources

authors： Bertrand B,Alburaki M,Legout H,Moulin S,Mougel F,Garnery L

更新日期：2015-05-01 00:00:00

abstract：:Crepidula convexa, a calyptreid gastropod with direct embryonic development, changes sex from male to female in the course of its lifetime (protandry). Under sex-allocation theory, male reproductive success should be independent from age and size (a proxy used for age). However, this may be counterbalanced by female c...

journal_title：Molecular ecology resources

authors： Daguin-Thiébaut C,LE Cam S,Viard F

更新日期：2009-05-01 00:00:00

abstract：:Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of...

journal_title：Molecular ecology resources

authors： Elliott CP

更新日期：2009-07-01 00:00:00

abstract：:Global climate change (GCC) may be causing distribution range shifts in many organisms worldwide. Multiple efforts are currently focused on the development of models to better predict distribution range shifts due to GCC. We addressed this issue by including intraspecific genetic structure and spatial autocorrelation ...

journal_title：Molecular ecology resources

authors： Martínez-Minaya J,Conesa D,Fortin MJ,Alonso-Blanco C,Picó FX,Marcer A

更新日期：2019-07-01 00:00:00

abstract：:We developed a total of 50 microsatellite markers for the three fungal pathogens causing the most important leaf spot diseases of banana: 32 loci for Mycosphaerella fijiensis are presented, and nine loci each for Mycosphaerella musicola and Mycosphaerella eumusae. All these loci were polymorphic within each species on...

journal_title：Molecular ecology resources

authors： Zapater MF,Duchemin M,Dussart JF,Coste D,Brottier P,Carlier J

更新日期：2008-09-01 00:00:00

abstract：:Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...

journal_title：Molecular ecology resources

authors： Rodríguez-Ezpeleta N,Bradbury IR,Mendibil I,Álvarez P,Cotano U,Irigoien X

更新日期：2016-07-01 00:00:00

abstract：:New DNA sequencing technologies are allowing researchers to explore the genomes of the millions of natural history specimens collected prior to the molecular era. Yet, we know little about how well specific next-generation sequencing (NGS) techniques work with the degraded DNA typically extracted from museum specimens...

journal_title：Molecular ecology resources

authors： McCormack JE,Tsai WL,Faircloth BC

更新日期：2016-09-01 00:00:00

abstract：:Knowledge of population demographics is important for species management but can be challenging in low-density, wide-ranging species. Population monitoring of the endangered Sonoran pronghorn (Antilocapra americana sonoriensis) is critical for assessing the success of recovery efforts, and noninvasive DNA sampling (ND...

journal_title：Molecular ecology resources

authors： Woodruff SP,Johnson TR,Waits LP

更新日期：2015-07-01 00:00:00

abstract：:Thirteen polymorphic microsatellite markers were isolated and characterized from the great hornbill, Buceros bicornis. In analyses of 20 individuals, the numbers of alleles per locus varied from two to 11. The expected and observed heterozygosities ranged from 0.22 to 0.88 and from 0.20 to 1.00, respectively. The mean...

journal_title：Molecular ecology resources

authors： Chamutpong S,Saito DS,Viseshakul N,Nishiumi I,Poonswad P,Ponglikitmongkol M

更新日期：2009-03-01 00:00:00

abstract：:In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heter...

journal_title：Molecular ecology resources

authors： Somta P,Musch W,Kongsamai B,Chanprame S,Nakasathien S,Toojinda T,Sorajjapinun W,Seehalak W,Tragoonrung S,Srinives P

更新日期：2008-09-01 00:00:00

abstract：:In the Alectoris partridges (Phasianidae), hybridization occurs occasionally as a result of the natural breakdown of isolating mechanisms but more frequently as a result of human activity. No genetic record of hybridization is known for the barbary partridge (A. barbara). This species is distributed mostly in North Af...

journal_title：Molecular ecology resources

authors： Barbanera F,Guerrini M,Bertoncini F,Cappelli F,Muzzeddu M,Dini F

更新日期：2011-01-01 00:00:00

abstract：:The brushtail possum is a major agricultural and ecological pest in New Zealand. A novel noninvasive DNA sampling tool for detecting its presence (WaxTags, or WT) was tested. DNA was recovered from saliva left on WT, and two lengths (407 bp and 648 bp) of the cytochrome c oxidase I (COI) barcoding region were amplifie...

journal_title：Molecular ecology resources

authors： Vargas ML,Cruickshank RH,Ross JG,Holyoake AJ,Ogilvie SC,Paterson AM

更新日期：2009-03-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) has formed the backbone of phylogeographic research for many years; however, recent trends focus on genome-wide analyses. One method proposed for calibrating inferences from noisy next-generation data, such as RAD sequencing, is to compare these results with analyses of mitochondrial sequence...

journal_title：Molecular ecology resources

authors： Stobie CS,Cunningham MJ,Oosthuizen CJ,Bloomer P

更新日期：2019-01-01 00:00:00

abstract：:Genetic stock identification (GSI) is an important tool in fisheries management. Microsatellites (μSATs) have been the dominant genetic marker for GSI; however, increasing availability and numerous advantages of single-nucleotide polymorphism (SNP) markers make them an appealing alternative. We tested performance of 1...

journal_title：Molecular ecology resources

authors： Hess JE,Matala AP,Narum SR

更新日期：2011-03-01 00:00:00

abstract：:Aleppo pine (Pinus halepensis Mill.) is a relevant conifer species for studying adaptive responses to drought and fire regimes in the Mediterranean region. In this study, we performed Illumina next-generation sequencing of two phenotypically divergent Aleppo pine accessions with the aims of (i) characterizing the tran...

journal_title：Molecular ecology resources

authors： Pinosio S,González-Martínez SC,Bagnoli F,Cattonaro F,Grivet D,Marroni F,Lorenzo Z,Pausas JG,Verdú M,Vendramin GG

更新日期：2014-07-01 00:00:00

abstract：:Museum collections are essential for reconstructing and understanding past biodiversity. Many museum specimens are, however, challenging to identify. Museum samples may be incomplete, have an unusual morphology, or represent juvenile individuals, all of which complicate accurate identification. In some cases, inaccura...

journal_title：Molecular ecology resources

authors： Vershinina AO,Kapp JD,Baryshnikov GF,Shapiro B

更新日期：2020-09-01 00:00:00

abstract：:Inferring the mode and tempo of natural selection helps further our understanding of adaptation to past environmental changes. Here, we introduce McSwan, a method to detect and date past and recent natural selection events in the case of a hard sweep. The method is based on the comparison of site frequency spectra obt...

journal_title：Molecular ecology resources

authors： Tournebize R,Poncet V,Jakobsson M,Vigouroux Y,Manel S

更新日期：2019-01-01 00:00:00

abstract：:A genomic library was constructed and 13 polymorphic microsatellite markers were developed for Hakea oldfieldii, a woody shrub endemic to southwest Western Australia. Polymorphism was investigated for these markers in 28 individuals from a single population located in restricted habitat at the base of the Whicher Rang...

journal_title：Molecular ecology resources

authors： Byrne M,Hankinson M

更新日期：2009-07-01 00:00:00

abstract：:In order to facilitate the addition of a genetic component to conservation management plans for Georgia false indigo (Amorpha georgiana var. georgiana), a rare legume of the southeastern USA, 12 polymorphic microsatellite markers were developed. No gametic disequilibrium was detected among locus pairs, but observation...

journal_title：Molecular ecology resources

authors： Straub SC,Bogdanowicz SM,Doyle JJ

更新日期：2009-01-01 00:00:00

abstract：:Eight polymorphic microsatellite loci were isolated and characterized from a microsatellite DNA-enriched DNA library for the leopard coralgrouper (Plectropomus leopardus Lacepède, 1802), a popular food fish in the East Indies. These loci showed polymorphism information content ranging from 0.493 to 0.854, allele numbe...

journal_title：Molecular ecology resources

authors： Ding SX,Zeng HS,Wang Y,Pan Y,Shi XF

更新日期：2009-11-01 00:00:00

abstract：:To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of gen...

journal_title：Molecular ecology resources

authors： Murata C,Ogura G,Kuroiwa A

更新日期：2011-03-01 00:00:00

abstract：:The development of genetic markers has revolutionized molecular studies within and among populations. Although poly-allelic microsatellites are the most commonly used genetic marker for within-population studies of free-living animals, biallelic single nucleotide polymorphisms, or SNPs, have also emerged as a viable o...

journal_title：Molecular ecology resources

authors： Weinman LR,Solomon JW,Rubenstein DR

更新日期：2015-05-01 00:00:00

abstract：:A variety of challenges arise when monitoring wildlife populations for disease. Sampling tissues can be invasive to hosts, and obtaining sufficient sample sizes can be expensive and time-consuming, particularly for rare species and when pathogen prevalence is low. Environmental DNA (eDNA)-based detection of pathogens ...

journal_title：Molecular ecology resources

authors： Hall EM,Crespi EJ,Goldberg CS,Brunner JL

更新日期：2016-03-01 00:00:00

abstract：:DNA-based methods have greatly enhanced the sensitivity and specificity of hematophagous arthropod bloodmeal identification. A variety of methods have been applied to study the blood-feeding behaviour of mosquitoes, ticks, black flies and other blood-feeding arthropods as it relates to host-parasite interactions and p...

journal_title：Molecular ecology resources

authors： Kent RJ

更新日期：2009-01-01 00:00:00

abstract：:QST , a measure of quantitative genetic differentiation among populations, is an index that can suggest local adaptation if QST for a trait is sufficiently larger than the mean FST of neutral genetic markers. A previous method by Whitlock and Guillaume derived a simulation resampling approach to statistically test for...

journal_title：Molecular ecology resources

authors： Gilbert KJ,Whitlock MC

更新日期：2015-03-01 00:00:00

abstract：:DNA metabarcoding was utilized for a large-scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count-based biodiversities revealed clustering in concordance with whether collection sites were located ins...

journal_title：Molecular ecology resources

authors： Hardulak LA,Morinière J,Hausmann A,Hendrich L,Schmidt S,Doczkal D,Müller J,Hebert PDN,Haszprunar G

更新日期：2020-11-01 00:00:00

abstract：:Molecular genetic estimates of population effective size (Ne ) lose accuracy and precision when insufficient numbers of samples or loci are used. Ideally, researchers would like to forecast the necessary power when planning their project. neogen (genetic Ne for Overlapping Generations) enables estimates of precision a...

journal_title：Molecular ecology resources

authors： Blower DC,Riginos C,Ovenden JR

更新日期：2019-01-01 00:00:00

abstract：:Studies on the biogeography of host-parasitoid interactions are scarce, mainly because of technical difficulties associated with rearing and species identification. DNA barcoding is increasingly recognized as a valuable tool for taxon identification, allowing to link different life history stages of a species. We eval...

journal_title：Molecular ecology resources

authors： Santos AM,Besnard G,Quicke DL

更新日期：2011-01-01 00:00:00