The case of an arctic wild ass highlights the utility of ancient DNA for validating problematic identifications in museum collections.

abstract：:Global climate change (GCC) may be causing distribution range shifts in many organisms worldwide. Multiple efforts are currently focused on the development of models to better predict distribution range shifts due to GCC. We addressed this issue by including intraspecific genetic structure and spatial autocorrelation ...

journal_title：Molecular ecology resources

authors： Martínez-Minaya J,Conesa D,Fortin MJ,Alonso-Blanco C,Picó FX,Marcer A

更新日期：2019-07-01 00:00:00

abstract：:DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR o...

journal_title：Molecular ecology resources

authors： Dittrich-Schröder G,Wingfield MJ,Klein H,Slippers B

更新日期：2012-01-01 00:00:00

abstract：:DNA sourced from faeces is notoriously less reliable than that from tissue. Hence, understanding whether faecal pellet quality varies within faecal piles may be important for sample selection. We hypothesized that the order in which faecal pellets are deposited may influence microsatellite polymerase chain reaction (P...

journal_title：Molecular ecology resources

authors： Walker FM,Horsup A,Taylor AC

更新日期：2009-05-01 00:00:00

abstract：:Aleppo pine (Pinus halepensis Mill.) is a relevant conifer species for studying adaptive responses to drought and fire regimes in the Mediterranean region. In this study, we performed Illumina next-generation sequencing of two phenotypically divergent Aleppo pine accessions with the aims of (i) characterizing the tran...

journal_title：Molecular ecology resources

authors： Pinosio S,González-Martínez SC,Bagnoli F,Cattonaro F,Grivet D,Marroni F,Lorenzo Z,Pausas JG,Verdú M,Vendramin GG

更新日期：2014-07-01 00:00:00

abstract：:The rice leaffolder, Cnaphalocrocis medinalis Guenée (Crambidae, Lepidoptera), is an important agricultural pest that causes serious losses to rice production in rice-growing regions with high humidity and temperature. However, a lack of genomic resources limits in-depth understanding of its biological characteristics...

journal_title：Molecular ecology resources

authors： Zhao X,Xu H,He K,Shi Z,Chen X,Ye X,Mei Y,Yang Y,Li M,Gao L,Xu L,Xiao H,Liu Y,Lu Z,Li F

更新日期：2021-02-01 00:00:00

abstract：:We isolated 18 microsatellites from Sycoscapter australis, a nonpollinating fig wasp that develops in figs of Ficus macrophylla, and assessed their variability in 20 wasps. We further optimized nine of these loci for use in three other Sycoscapter species that develop in Ficus rubiginosa figs and assessed their variab...

journal_title：Molecular ecology resources

authors： Bouteiller-Reuter C,Hale ML,Cook JM

更新日期：2009-05-01 00:00:00

abstract：:Recent advances in high-throughput sequencing technologies have offered the possibility to generate genomewide sequence data to delineate previously unidentified genetic structure, obtain more accurate estimates of demographic parameters and to evaluate potential adaptive divergence. Here, we identified 27 556 single ...

journal_title：Molecular ecology resources

authors： Zhang BD,Xue DX,Wang J,Li YL,Liu BJ,Liu JX

更新日期：2016-05-01 00:00:00

abstract：:Luffa spp. (sponge gourd or ridge gourd) is an economically important vegetable crop widely cultivated in China, India and Southeast Asia. Here, we employed PacBio long-read single-molecule real-time (SMRT) sequencing to perform de novo genome assemblies of two commonly cultivated Luffa species, L. acutangula and L. c...

journal_title：Molecular ecology resources

authors： Pootakham W,Sonthirod C,Naktang C,Nawae W,Yoocha T,Kongkachana W,Sangsrakru D,Jomchai N,U-Thoomporn S,Sheedy JR,Buaboocha J,Mekiyanon S,Tangphatsornruang S

更新日期：2021-01-01 00:00:00

abstract：:Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183...

journal_title：Molecular ecology resources

authors： Cruz VP,Vera M,Pardo BG,Taggart J,Martinez P,Oliveira C,Foresti F

更新日期：2017-05-01 00:00:00

abstract：:The formation of chimeric sequences can create significant methodological bias in PCR-based DNA metabarcoding analyses. During mixed-template amplification of barcoding regions, chimera formation is frequent and well documented. However, profiling of fungal communities typically uses the more variable rDNA region ITS....

journal_title：Molecular ecology resources

authors： Bjørnsgaard Aas A,Davey ML,Kauserud H

更新日期：2017-07-01 00:00:00

abstract：:The interbreeding of individuals coming from genetically differentiated but incompletely isolated populations can lead to the formation of admixed populations, having important implications in ecology and evolution. In this simulation study, we evaluate how individual admixture proportions estimated by the software st...

journal_title：Molecular ecology resources

authors： Toyama KS,Crochet PA,Leblois R

更新日期：2020-11-01 00:00:00

abstract：:We developed 18 polymorphic microsatellite markers for Coregonus lavaretus from genomic libraries enriched for (GACA)(n) and (GATA)(n) repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats. These loci were screened in 69 individuals from two alpine populations in Austria. Allelic vari...

journal_title：Molecular ecology resources

authors： Winkler KA,Weiss S

更新日期：2008-09-01 00:00:00

abstract：:To characterize microbiomes and other ecological assemblages, ecologists routinely sequence and compare loci that differ among focal taxa. Counts of these sequences convey information regarding the occurrence and relative abundances of taxa, but provide no direct measure of their absolute abundances, due to the techni...

journal_title：Molecular ecology resources

authors： Harrison JG,John Calder W,Shuman B,Alex Buerkle C

更新日期：2021-01-01 00:00:00

abstract：:This study reports the isolation and characterization of 11 polymorphic microsatellites from a sand smelt (Atherina boyeri) genomic library. Enrichment was performed with di-, tri- and tetranucleotide motifs following the FIASCO procedure (fast isolation by AFLP of sequences containing repeats). All loci were found to...

journal_title：Molecular ecology resources

authors： Milana V,Sola L,Rossi AR,Barbisan F,Congiu L

更新日期：2009-05-01 00:00:00

abstract：:Crepidula convexa, a calyptreid gastropod with direct embryonic development, changes sex from male to female in the course of its lifetime (protandry). Under sex-allocation theory, male reproductive success should be independent from age and size (a proxy used for age). However, this may be counterbalanced by female c...

journal_title：Molecular ecology resources

authors： Daguin-Thiébaut C,LE Cam S,Viard F

更新日期：2009-05-01 00:00:00

abstract：:We developed 11 polymorphic microsatellite DNA markers for an Australian native plant, Daviesia triflora. The number of alleles per locus in 40 individuals varied from four to 19, and observed and expected heterozygosities ranged from 0.450 to 0.925 and from 0.497 to 0.899, respectively. Nine loci showed no significan...

journal_title：Molecular ecology resources

authors： He T,Krauss SL,Lamont BB

更新日期：2008-11-01 00:00:00

abstract：:Interpretation of high-throughput sequence data requires an understanding of how decisions made during bioinformatic data processing can influence results. One source of bias that is often cited is PCR clones (or PCR duplicates). PCR clones are common in restriction site-associated sequencing (RAD-seq) data sets, whic...

journal_title：Molecular ecology resources

authors： Euclide PT,McKinney GJ,Bootsma M,Tarsa C,Meek MH,Larson WA

更新日期：2020-01-01 00:00:00

abstract：:Germplasm collections provide an extremely valuable resource for breeders and researchers. However, misclassification of accessions by species often hinders the effective use of these collections. We propose that use of high-throughput genotyping tools can provide a fast, efficient and cost-effective way of confirming...

journal_title：Molecular ecology resources

authors： Mason AS,Zhang J,Tollenaere R,Vasquez Teuber P,Dalton-Morgan J,Hu L,Yan G,Edwards D,Redden R,Batley J

更新日期：2015-09-01 00:00:00

abstract：:Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was a...

journal_title：Molecular ecology resources

authors： Yang W,Zhang L,Mandáková T,Huang L,Li T,Jiang J,Yang Y,Lysak MA,Liu J,Hu Q

更新日期：2020-11-05 00:00:00

abstract：:In noninvasive genetic sampling, when genotyping error rates are high and recapture rates are low, misidentification of individuals can lead to overestimation of population size. Thus, estimating genotyping errors is imperative. Nonetheless, conducting multiple polymerase chain reactions (PCRs) at multiple loci is tim...

journal_title：Molecular ecology resources

authors： Hansen H,Ben-David M,McDonald DB

更新日期：2008-03-01 00:00:00

abstract：:Photosynthetic eukaryotes have a critical role as the main producers in most ecosystems of the biosphere. The ongoing environmental metabarcoding revolution opens the perspective for holistic ecosystems biological studies of these organisms, in particular the unicellular microalgae that often lack distinctive morpholo...

journal_title：Molecular ecology resources

authors： Decelle J,Romac S,Stern RF,Bendif el M,Zingone A,Audic S,Guiry MD,Guillou L,Tessier D,Le Gall F,Gourvil P,Dos Santos AL,Probert I,Vaulot D,de Vargas C,Christen R

更新日期：2015-11-01 00:00:00

abstract：:Sex-determining modes remain unknown in numerous species, notably in fishes, in which a variety of modalities have been reported. Additionally, noninvasive individual sexing is problematic for species without external sex attributes or for early life stages, requiring cytogenetic or molecular analyses when sex chromos...

journal_title：Molecular ecology resources

authors： Trenkel VM,Boudry P,Verrez-Bagnis V,Lorance P

更新日期：2020-11-01 00:00:00

abstract：:The 'Symphyta' is a paraphyletic assemblage at the base of the order Hymenoptera, comprising 14 families and about 8750 species. All have phytophagous larvae, except for the Orussidae, which are parasitoids. This study presents and evaluates the results of DNA barcoding of approximately 5360 specimens of 'Symphyta', m...

journal_title：Molecular ecology resources

authors： Schmidt S,Taeger A,Morinière J,Liston A,Blank SM,Kramp K,Kraus M,Schmidt O,Heibo E,Prous M,Nyman T,Malm T,Stahlhut J

更新日期：2017-07-01 00:00:00

abstract：:Tracing maternal and paternal lineages independently to explore breeding systems and dispersal strategies in natural populations has been high on the wish-list of evolutionary biologists. As males are the heterogametic sex in mammals, such sex-specific patterns can be indirectly observed when Y chromosome polymorphism...

journal_title：Molecular ecology resources

authors： Greminger MP,Krützen M,Schelling C,Pienkowska-Schelling A,Wandeler P

更新日期：2010-05-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP) fingerprint data are now commonly collected using DNA sequencers. AFLP genotypes are still often scored by eye from such data - a time-consuming, error-prone and subjective process. We present a semi-automated method of genotyping sequencer-collected AFLPs at predefined fr...

journal_title：Molecular ecology resources

authors： Whitlock R,Hipperson H,Mannarelli M,Butlin RK,Burke T

更新日期：2008-07-01 00:00:00

abstract：:This article documents the addition of 153 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brassica oleracea, Brycon amazonicus, Dimorphandra wilsonii, Eupallasella percnurus, Helleborus foetidus, Ipomoea purpurea, Phrynops geoffroanus, Prochilodus...

journal_title：Molecular ecology resources

authors： Molecular Ecology Resources Primer Development Consortium.,Aksoy S,Almeida-Val VM,Azevedo VC,Baucom R,Bazaga P,Beheregaray LB,Bennetzen JL,Brassaloti RA,Burgess TI,Caccone A,Chang SM,Ciampi AY,Ciancaleoni S,Clímaco GT,Cloue

更新日期：2013-03-01 00:00:00

abstract：:This article documents the whole genome sequence information of the Indian Zaprionus indianus, a member of the fruit fly family Drosophilidae. The sequences were generated on an Illumina platform and reads and whole genome sequence submitted to NCBI to the SRA and BioProject databases, respectively. This is the first ...

journal_title：Molecular ecology resources

authors： Khanna R,Mohanty S

更新日期：2017-05-01 00:00:00

abstract：:Evolutionary alterations to cis-regulatory sequences are likely to cause adaptive phenotypic complexity, through orchestrating changes in cellular proliferation, identity and communication. For nonmodel organisms with adaptive key innovations, patterns of regulatory evolution have been predominantly limited to targete...

journal_title：Molecular ecology resources

authors： Kratochwil CF,Meyer A

更新日期：2015-07-01 00:00:00

abstract：:In this article, we describe ednaoccupancy, an r package for fitting Bayesian, multiscale occupancy models. These models are appropriate for occupancy surveys that include three nested levels of sampling: primary sample units within a study area, secondary sample units collected from each primary unit and replicates o...

journal_title：Molecular ecology resources

authors： Dorazio RM,Erickson RA

更新日期：2018-03-01 00:00:00

abstract：:The emu (Dromaius novaehollandiae), a member of ratite family, is native to Australia and has been introduced to other countries worldwide. In this work, 10 polymorphic microsatellite loci were isolated and characterized for emu from public sequences. Polymorphism was surveyed in 22 individuals from two different popu...

journal_title：Molecular ecology resources

authors： Yáñez JM,González R,Angulo J,Vidal R,Santos JL,Martínez V

更新日期：2008-11-01 00:00:00