Abstract:
:DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR of the cox 1 mitochondrial locus. A silica-membrane-based approach was the most successful, followed by a method using a combination of incubation buffers and a method using magnetic beads. The method using buffers was the most cost- and time effective. Using this method, larvae from Eucalyptus seed capsule galls could be assigned a role (parasitoid, gall former or inquiline) in the gall-inhabiting complex.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Dittrich-Schröder G,Wingfield MJ,Klein H,Slippers Bdoi
10.1111/j.1755-0998.2011.03074.xsubject
Has Abstractpub_date
2012-01-01 00:00:00pages
109-15issue
1eissn
1755-098Xissn
1755-0998journal_volume
12pub_type
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