Population structure of Atlantic mackerel inferred from RAD-seq-derived SNP markers: effects of sequence clustering parameters and hierarchical SNP selection.

abstract：:We report the development of 10 polymorphic molecular markers containing short tandem repeats in the cosmopolitan reef-building coral, Pocillopora damicornis, an important model species for coral health, physiology, ecology, and genetics. The availability of polymorphic DNA markers in P. damicornis can act as impetus ...

journal_title：Molecular ecology resources

authors： Starger CJ,Yeoh SS,Dai CF,Baker AC,Desalle R

更新日期：2008-05-01 00:00:00

abstract：:Microsatellite loci were identified from Cornus kousa'National'. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ra...

journal_title：Molecular ecology resources

authors： Wadl PA,Wang X,Scheffler BE,Rinehart TA,Trigiano RN

更新日期：2008-07-01 00:00:00

abstract：:The three-toed sloths (Bradypus) are slow-moving arboreal neotropical mammals. Understanding demographic variables (such as sex ratio) of populations is a key for conservation purposes. Nevertheless, gender assignment of Bradypus is particularly challenging because of the lack of sexual dimorphism in infants and in ad...

journal_title：Molecular ecology resources

authors： Martinelli AB,DE Moraes-Barros N,Alvarenga CS,Chaves PB,Santos LA,Fagundes V

更新日期：2010-07-01 00:00:00

abstract：:We report results of cross-species amplification in Dactylis glomerata and Lolium perenne of 12 simple sequence repeats (SSRs) isolated from Lolium multiflorum×Festuca glaucescens, 42 SSRs from Festuca arundinacea and two sequence tagged sites from Oryza sativa. We compared the transferability and diversity between D....

journal_title：Molecular ecology resources

authors： Litrico I,Bech N,Flajoulot S,Cadier D,Talon C,Gibelin C,Barre P

更新日期：2009-01-01 00:00:00

abstract：:Microsatellites are valuable markers for the analysis of genetic diversity, linkage mapping or genotyping. The limited availability of microsatellites for the genus Potentilla (Rosaceae) stipulated the isolation of markers from a representative (Potentilla pusilla Host) of the Potentilla core group that constitutes th...

journal_title：Molecular ecology resources

authors： Dobeš Ch,Scheffknecht S

更新日期：2012-07-01 00:00:00

abstract：:The spatial signature of microevolutionary processes structuring genetic variation may play an important role in the detection of loci under selection. However, the spatial location of samples has not yet been used to quantify this. Here, we present a new two-step method of spatial outlier detection at the individual ...

journal_title：Molecular ecology resources

authors： Wagner HH,Chávez-Pesqueira M,Forester BR

更新日期：2017-11-01 00:00:00

abstract：:Predicting whether a predator is capable of affecting the dynamics of a prey species in the field implies the analysis of the complete diet of the predator, not simply rates of predation on a target taxon. Here, we employed the Ion Torrent next-generation sequencing technology to investigate the diet of a generalist a...

journal_title：Molecular ecology resources

authors： Piñol J,San Andrés V,Clare EL,Mir G,Symondson WO

更新日期：2014-01-01 00:00:00

abstract：:The almendro (Dipteryx panamensis, Fabaceae) is a tetraploid tree native to the Atlantic lowland rainforests of Central America. We present nine microsatellite primer pairs amplified in three multiplexed reactions for 549 individuals from four sites in Costa Rica. All loci were polymorphic, ranging from three to 13 al...

journal_title：Molecular ecology resources

authors： Hanson TR,Brunsfeld SJ,Finegan B,Waits LP

更新日期：2008-03-01 00:00:00

abstract：:Historical samples, like tanned hides and trophy skulls, can be extremely important for genetic studies of endangered or elusive species. Selection of a sampling protocol that is likely to provide sufficient amount and quality of DNA with a minimum damage to the original specimen is often critical for a success of the...

journal_title：Molecular ecology resources

authors： Polanc P,Sindičić M,Jelenčič M,Gomerčić T,Kos I,Huber D

更新日期：2012-03-01 00:00:00

abstract：:Entomopathogenic fungi in the genus Metarhizium are useful for biological control programmes against economically important arthropod pests worldwide. However, understanding the true diversity and ecology of these organisms is hampered by convergent morphologies between species. The application of molecular techniques...

journal_title：Molecular ecology resources

authors： Kepler RM,Rehner SA

更新日期：2013-03-01 00:00:00

abstract：:Phylogenomic methods have proven useful for resolving deep nodes and recalcitrant groups in the spider tree of life. Across arachnids, transcriptomic approaches may generate thousands of loci, and target-capture methods, using the previously designed arachnid-specific probe set, can target a maximum of about 1,000 loc...

journal_title：Molecular ecology resources

authors： Kulkarni S,Wood H,Lloyd M,Hormiga G

更新日期：2020-01-01 00:00:00

abstract：:The construction of microsatellite-enriched libraries is an indispensable tool to search for molecular markers as complete genome sequences are still not available for the majority of species of interest. Numerous protocols are available in the literature for the construction of these libraries; however, sometimes the...

journal_title：Molecular ecology resources

authors： Techen N,Arias RS,Glynn NC,Pan Z,Khan IA,Scheffler BE

更新日期：2010-05-01 00:00:00

abstract：:The Chilean isopod Excirolana hirsuticauda is a marine benthic brooder with wide distributional range and low potential for long-distance dispersal. Eleven microsatellite markers were developed for E. hirsuticauda using enriched libraries. Characterization of those loci in 35 individuals from Playa Blanca beach showed...

journal_title：Molecular ecology resources

authors： Haye PA,Marchant S

更新日期：2008-09-01 00:00:00

abstract：:We developed 16 microsatellite markers in Cordia bifurcata, a Central and South American shrub. The markers show low polymorphism in C. bifurcata, a species suspected of self-fertilization or apomixis. Of four polymorphic loci, three had only two alleles. However, current research indicates that these markers hold val...

journal_title：Molecular ecology resources

authors： Spoon TR,Kesseli RV

更新日期：2008-09-01 00:00:00

abstract：:We describe a convenient, cost-effective and flexible medium-throughput single nucleotide polymorphism (SNP) genotyping method, Multiplex SNP-SCALE, which enables the simultaneous amplification by polymerase chain reaction (PCR) of up to 25 (or potentially more) loci followed by electrophoresis in an automated DNA seq...

journal_title：Molecular ecology resources

authors： Kenta T,Gratten J,Haigh NS,Hinten GN,Slate J,Butlin RK,Burke T

更新日期：2008-11-01 00:00:00

abstract：:Aleppo pine (Pinus halepensis Mill.) is a relevant conifer species for studying adaptive responses to drought and fire regimes in the Mediterranean region. In this study, we performed Illumina next-generation sequencing of two phenotypically divergent Aleppo pine accessions with the aims of (i) characterizing the tran...

journal_title：Molecular ecology resources

authors： Pinosio S,González-Martínez SC,Bagnoli F,Cattonaro F,Grivet D,Marroni F,Lorenzo Z,Pausas JG,Verdú M,Vendramin GG

更新日期：2014-07-01 00:00:00

abstract：:Single nucleotide polymorphisms (SNPs) have become the marker of choice for genetic studies in organisms of conservation, commercial or biological interest. Most SNP discovery projects in nonmodel organisms apply a strategy for identifying putative SNPs based on filtering rules that account for random sequencing error...

journal_title：Molecular ecology resources

authors： Gonçalves da Silva A,Barendse W,Kijas JW,Barris WC,McWilliam S,Bunch RJ,McCullough R,Harrison B,Hoelzel AR,England PR

更新日期：2015-07-01 00:00:00

abstract：:The European flounder (Platichthys flesus L.) is used in ecotoxicological studies to provide detailed information on the effects of pollution on individual fish. Data on population and evolutionary level effects are, however, limited. Here, the isolation and characterization of 28 novel species specific microsatellite...

journal_title：Molecular ecology resources

authors： Tysklind N,Neuparth T,Ashcroft GR,Taylor MI,Lyons BP,McCarthy ID,Carvalho GR

更新日期：2009-05-01 00:00:00

abstract：:Sex-linked molecular markers have become valuable tools for understanding sex ratio evolution and sex-specific physiology in pre-reproductive plants. To develop new accurate methods for sexing Distichlis spicata juveniles and nonflowering individuals, we converted a random amplified polymorphic DNA-polymerase chain re...

journal_title：Molecular ecology resources

authors： Eppley SM,O'Quinn R,Brown AL

更新日期：2009-09-01 00:00:00

abstract：:A microsatellite-enriched genomic library was obtained for the endangered Japanese loach Leptobotia curta, and 39 dinucleotide markers were successfully isolated and characterized. These markers had between one and nine alleles, with expected heterozygosity ranging from 0 to 0.839, in a population from the Lake Biwa-Y...

journal_title：Molecular ecology resources

authors： Watanabe K,Takeshima H,Iwata A,Abe T,Uehara K,Kakioka R,Kihira D,Nishida M

更新日期：2008-01-01 00:00:00

abstract：:New DNA sequencing technologies are allowing researchers to explore the genomes of the millions of natural history specimens collected prior to the molecular era. Yet, we know little about how well specific next-generation sequencing (NGS) techniques work with the degraded DNA typically extracted from museum specimens...

journal_title：Molecular ecology resources

authors： McCormack JE,Tsai WL,Faircloth BC

更新日期：2016-09-01 00:00:00

abstract：:Twelve polymorphic microsatellite loci were isolated for the bonefish, Albula vulpes using a polymerase chain reaction-based procedure. The number of alleles ranged from two to 23 (mean = 8.8) in 37 specimens from south Florida. Observed and expected heterozygosities ranged from 0.07 to 0.77 (mean = 0.42) and from 0.0...

journal_title：Molecular ecology resources

authors： Seyoum S,Wallace EM,Tringali MD

更新日期：2008-03-01 00:00:00

abstract：:Publilia concava is an eastern North American membracid commonly occurring in large but spatially patchy aggregations, primarily on the host plant Solidago altissima. Like other myrmecophiles, P. concava provides sugary excretions to ants in return for the various protective, competitive or even sanitary benefits that...

journal_title：Molecular ecology resources

authors： Chhatre V,Morales MA,Abbot P

更新日期：2009-07-01 00:00:00

abstract：:Adaptation to different ecological environments can promote speciation. Although numerous examples of such 'ecological speciation' now exist, the genomic basis of the process, and the role of gene flow in it, remains less understood. This is, at least in part, because systems that are well characterized in terms of th...

journal_title：Molecular ecology resources

authors： Comeault AA,Sommers M,Schwander T,Buerkle CA,Farkas TE,Nosil P,Parchman TL

更新日期：2012-05-01 00:00:00

abstract：:A significant portion of plant species are polyploids, with ploidy levels sometimes varying among individuals and/or populations. Current techniques to determine the individual ploidy, e.g., flow cytometry, chromosome counting or genotyping-by-sequencing, are often cumbersome. Based on the genotypic probabilities for ...

journal_title：Molecular ecology resources

authors： Huang K,Dunn DW,Li Z,Zhang P,Dai Y,Li B

更新日期：2019-09-01 00:00:00

abstract：:Sequence-based specimen identification, known as DNA barcoding, is a common method complementing traditional morphology-based taxonomic assignments. The fundamental resource in DNA barcoding is the availability of a taxonomically reliable sequence database to use as a reference for sequence comparisons. Here, we provi...

journal_title：Molecular ecology resources

authors： Barco A,Raupach MJ,Laakmann S,Neumann H,Knebelsberger T

更新日期：2016-01-01 00:00:00

abstract：:With the advent of next generation sequencing technologies, transcriptome level sequence collections are arising as prominent resources for the discovery of gene-based molecular markers. In a previous study more than 15,000 simple sequence repeats (SSRs) in expressed sequence tag (EST) sequences resulting from 454 pyr...

journal_title：Molecular ecology resources

authors： Lesser MR,Parchman TL,Buerkle CA

更新日期：2012-05-01 00:00:00

abstract：:We developed 18 polymorphic microsatellite markers for Coregonus lavaretus from genomic libraries enriched for (GACA)(n) and (GATA)(n) repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats. These loci were screened in 69 individuals from two alpine populations in Austria. Allelic vari...

journal_title：Molecular ecology resources

authors： Winkler KA,Weiss S

更新日期：2008-09-01 00:00:00

abstract：:Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alle...

journal_title：Molecular ecology resources

authors： Anderson SJ,Stone CL,Posada-Buitrago ML,Boore JL,Neelam BA,Stephens RM,Luster DG,Frederick RD,Pedley KF

更新日期：2008-11-01 00:00:00

abstract：:Mosquitoes belonging to the Culex pipiens complex are primary vectors for diseases such as West Nile encephalitis, Eastern equine encephalitis, many arboviruses, as well as lymphatic filariases. Despite sharing physiological characteristics, each mosquito species within the Culex complex has unique behavioural and rep...

journal_title：Molecular ecology resources

authors： Kang D,Sim C

更新日期：2013-05-01 00:00:00