Inference of individual ploidy level using codominant markers.

Abstract:

:A significant portion of plant species are polyploids, with ploidy levels sometimes varying among individuals and/or populations. Current techniques to determine the individual ploidy, e.g., flow cytometry, chromosome counting or genotyping-by-sequencing, are often cumbersome. Based on the genotypic probabilities for polysomic inheritance under double-reduction, we developed a model to estimate allele frequency and infer the ploidy status of individuals from the allelic phenotypes of codominant genetic markers. The allele frequencies are estimated by an expectation-maximization algorithm in the presence of null alleles, false alleles, negative amplifications and self-fertilization, and the posterior probabilities are used to assign individuals into different levels of ploidy. The accuracy of this method under different conditions is evaluated. Our methods are freely available in a new software package, ploidyinfer, for use by other researchers which can be downloaded from http://github.com/huangkang1987/ploidyinfer.

journal_name

Mol Ecol Resour

authors

Huang K,Dunn DW,Li Z,Zhang P,Dai Y,Li B

doi

10.1111/1755-0998.13032

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

1218-1229

issue

5

eissn

1755-098X

issn

1755-0998

journal_volume

19

pub_type

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