De novo characterization of the Timema cristinae transcriptome facilitates marker discovery and inference of genetic divergence.

abstract：:Interpretation of high-throughput sequence data requires an understanding of how decisions made during bioinformatic data processing can influence results. One source of bias that is often cited is PCR clones (or PCR duplicates). PCR clones are common in restriction site-associated sequencing (RAD-seq) data sets, whic...

journal_title：Molecular ecology resources

authors： Euclide PT,McKinney GJ,Bootsma M,Tarsa C,Meek MH,Larson WA

更新日期：2020-01-01 00:00:00

abstract：:Many plants and some animal species are polyploids. Nondisomically inherited markers (e.g. microsatellites) in such species cannot be analysed directly by standard population genetics methods developed for diploid species. One solution is to transform the polyploid codominant genotypes to pseudodiploid-dominant genoty...

journal_title：Molecular ecology resources

authors： Wang J,Scribner KT

更新日期：2014-05-01 00:00:00

abstract：:A variety of challenges arise when monitoring wildlife populations for disease. Sampling tissues can be invasive to hosts, and obtaining sufficient sample sizes can be expensive and time-consuming, particularly for rare species and when pathogen prevalence is low. Environmental DNA (eDNA)-based detection of pathogens ...

journal_title：Molecular ecology resources

authors： Hall EM,Crespi EJ,Goldberg CS,Brunner JL

更新日期：2016-03-01 00:00:00

abstract：:Exon markers have a long history of use in phylogenetics of ray-finned fishes, the most diverse clade of vertebrates with more than 35,000 species. As the number of published genomes increases, it has become easier to test exons and other genetic markers for signals of ancient duplication events and filter out paralog...

journal_title：Molecular ecology resources

authors： Hughes LC,Ortí G,Saad H,Li C,White WT,Baldwin CC,Crandall KA,Arcila D,Betancur-R R

更新日期：2020-10-21 00:00:00

abstract：:The emu (Dromaius novaehollandiae), a member of ratite family, is native to Australia and has been introduced to other countries worldwide. In this work, 10 polymorphic microsatellite loci were isolated and characterized for emu from public sequences. Polymorphism was surveyed in 22 individuals from two different popu...

journal_title：Molecular ecology resources

authors： Yáñez JM,González R,Angulo J,Vidal R,Santos JL,Martínez V

更新日期：2008-11-01 00:00:00

abstract：:Thirty-four novel microsatellite markers developed for wood cricket (Nemobius sylvestris) were tested and optimized. Twenty-five microsatellite loci were polymorphic, exhibiting between two and nine alleles. Observed heterozygosities ranged from 0.038 to 0.925. The microsatellites were also tested in a species belongi...

journal_title：Molecular ecology resources

authors： Vanhala T,Cottrell J

更新日期：2008-11-01 00:00:00

abstract：:The emergence of third-generation sequencing (3GS; long-reads) is bringing closer the goal of chromosome-size fragments in de novo genome assemblies. This allows the exploration of new and broader questions on genome evolution for a number of nonmodel organisms. However, long-read technologies result in higher sequenc...

journal_title：Molecular ecology resources

authors： Jaworski CC,Allan CW,Matzkin LM

更新日期：2020-09-01 00:00:00

abstract：:High-throughput identification technologies provide efficient tools for understanding the ecology and functioning of microorganisms. Yet, these methods have been only rarely used for monitoring and testing ecological hypotheses in plant pathogens and pests in spite of their immense importance in agriculture, forestry ...

journal_title：Molecular ecology resources

authors： Tedersoo L,Drenkhan R,Anslan S,Morales-Rodriguez C,Cleary M

更新日期：2019-01-01 00:00:00

abstract：:Camelids are characterized by their unique adaptive immune system that exhibits the generation of homodimeric heavy-chain immunoglobulins, somatic hypermutation of T-cell receptors, and low genetic diversity of major histocompatibility complex (MHC) genes. However, short-read assemblies are typically highly fragmented...

journal_title：Molecular ecology resources

authors： Ming L,Wang Z,Yi L,Batmunkh M,Liu T,Siren D,He J,Juramt N,Jambl T,Li Y,Jirimutu

更新日期：2020-05-01 00:00:00

abstract：:We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymo...

journal_title：Molecular ecology resources

authors： Degner JF,Hether TD,Hoffman EA

更新日期：2009-03-01 00:00:00

abstract：:In this article, we describe ednaoccupancy, an r package for fitting Bayesian, multiscale occupancy models. These models are appropriate for occupancy surveys that include three nested levels of sampling: primary sample units within a study area, secondary sample units collected from each primary unit and replicates o...

journal_title：Molecular ecology resources

authors： Dorazio RM,Erickson RA

更新日期：2018-03-01 00:00:00

abstract：:The rice stem borer, Chilo suppressalis, is one of the most damaging insect pests to rice production worldwide. Although C. suppressalis has been the focus of numerous studies examining cold tolerance and diapause, plant-insect interactions, pesticide targets and resistance, and the development of RNAi-mediated pest m...

journal_title：Molecular ecology resources

authors： Ma W,Zhao X,Yin C,Jiang F,Du X,Chen T,Zhang Q,Qiu L,Xu H,Joe Hull J,Li G,Sung WK,Li F,Lin Y

更新日期：2020-01-01 00:00:00

abstract：:The rice leaffolder, Cnaphalocrocis medinalis Guenée (Crambidae, Lepidoptera), is an important agricultural pest that causes serious losses to rice production in rice-growing regions with high humidity and temperature. However, a lack of genomic resources limits in-depth understanding of its biological characteristics...

journal_title：Molecular ecology resources

authors： Zhao X,Xu H,He K,Shi Z,Chen X,Ye X,Mei Y,Yang Y,Li M,Gao L,Xu L,Xiao H,Liu Y,Lu Z,Li F

更新日期：2021-02-01 00:00:00

abstract：:Eleven microsatellites were characterized for Semicossyphus pulcher (California sheephead) using an enrichment protocol. The number of alleles varied from three to 14 for a sample of 40 individuals from two populations. Expected heterozygosities ranged from 0.311 to 0.891. All loci but one were in Hardy-Weinberg equil...

journal_title：Molecular ecology resources

authors： Poortvliet M,Olsen JL,Selkoe KA,Coyer JA,Bernardi G

更新日期：2009-01-01 00:00:00

abstract：:DNA sourced from faeces is notoriously less reliable than that from tissue. Hence, understanding whether faecal pellet quality varies within faecal piles may be important for sample selection. We hypothesized that the order in which faecal pellets are deposited may influence microsatellite polymerase chain reaction (P...

journal_title：Molecular ecology resources

authors： Walker FM,Horsup A,Taylor AC

更新日期：2009-05-01 00:00:00

abstract：:We developed 18 polymorphic microsatellite markers for Coregonus lavaretus from genomic libraries enriched for (GACA)(n) and (GATA)(n) repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats. These loci were screened in 69 individuals from two alpine populations in Austria. Allelic vari...

journal_title：Molecular ecology resources

authors： Winkler KA,Weiss S

更新日期：2008-09-01 00:00:00

abstract：:We present the leaf and floral transcriptomes of two hybridizing bromeliad species that differ in their major pollinator systems. Here we identified candidate genes responsible for pollinator attraction and reproductive isolation in these two species. We searched for candidate genes involved in floral traits, such as ...

journal_title：Molecular ecology resources

authors： Palma-Silva C,Ferro M,Bacci M,Turchetto-Zolet AC

更新日期：2016-07-01 00:00:00

abstract：:An enriched genomic library was constructed and 9 novel polymorphic tetranucleotide microsatellite markers developed for Austrocedrus chilensis, the most economically important native conifer in the Andean Patagonian region. Polymorphism was investigated for these markers in 48 individuals from two populations. Number...

journal_title：Molecular ecology resources

authors： Arana MV,Buonamici A,Sebastiani F,Alia R,Gallo LA,Marchelli P,Moreno C,Vendramin GG

更新日期：2008-07-01 00:00:00

abstract：:Metabarcoding is increasingly used to assess species diversity by high-throughput sequencing where millions of sequences can be generated in parallel and multiple samples can be analysed in one sequencing run. Generating amplified fragments with a unique sequence identifier ('tag') for each sample is crucial, as it al...

journal_title：Molecular ecology resources

authors： Zizka VMA,Elbrecht V,Macher JN,Leese F

更新日期：2019-07-01 00:00:00

abstract：:Chimpanzee populations are diminishing as a consequence of human activities, and as a result this species is now endangered. In the context of conservation programmes, genetic data can add vital information, for instance on the genetic diversity and structure of threatened populations. Single nucleotide polymorphisms ...

journal_title：Molecular ecology resources

authors： Andrés O,Rönn AC,Bonhomme M,Kellermann T,Crouau-Roy B,Doxiadis G,Verschoor EJ,Goossens B,Domingo-Roura X,Bruford MW,Bosch M,Syvänen AC

更新日期：2008-05-01 00:00:00

abstract：:A significant portion of plant species are polyploids, with ploidy levels sometimes varying among individuals and/or populations. Current techniques to determine the individual ploidy, e.g., flow cytometry, chromosome counting or genotyping-by-sequencing, are often cumbersome. Based on the genotypic probabilities for ...

journal_title：Molecular ecology resources

authors： Huang K,Dunn DW,Li Z,Zhang P,Dai Y,Li B

更新日期：2019-09-01 00:00:00

abstract：:The brushtail possum is a major agricultural and ecological pest in New Zealand. A novel noninvasive DNA sampling tool for detecting its presence (WaxTags, or WT) was tested. DNA was recovered from saliva left on WT, and two lengths (407 bp and 648 bp) of the cytochrome c oxidase I (COI) barcoding region were amplifie...

journal_title：Molecular ecology resources

authors： Vargas ML,Cruickshank RH,Ross JG,Holyoake AJ,Ogilvie SC,Paterson AM

更新日期：2009-03-01 00:00:00

abstract：:We describe a convenient, cost-effective and flexible medium-throughput single nucleotide polymorphism (SNP) genotyping method, Multiplex SNP-SCALE, which enables the simultaneous amplification by polymerase chain reaction (PCR) of up to 25 (or potentially more) loci followed by electrophoresis in an automated DNA seq...

journal_title：Molecular ecology resources

authors： Kenta T,Gratten J,Haigh NS,Hinten GN,Slate J,Butlin RK,Burke T

更新日期：2008-11-01 00:00:00

abstract：:Knowledge of population demographics is important for species management but can be challenging in low-density, wide-ranging species. Population monitoring of the endangered Sonoran pronghorn (Antilocapra americana sonoriensis) is critical for assessing the success of recovery efforts, and noninvasive DNA sampling (ND...

journal_title：Molecular ecology resources

authors： Woodruff SP,Johnson TR,Waits LP

更新日期：2015-07-01 00:00:00

abstract：:Caribbean reef-building corals in the genus Acropora have been declining dramatically since the 1980s and are now listed as threatened. The study of their complex reproductive system (mixed asexual and sexual) and their population structure requires highly polymorphic nuclear genetic markers. Of eight previously devel...

journal_title：Molecular ecology resources

authors： Baums IB,Devlin-Durante MK,Brown L,Pinzón JH

更新日期：2009-07-01 00:00:00

abstract：:Plastid sequencing is an essential tool in the study of plant evolution. This high-copy organelle is one of the most technically accessible regions of the genome, and its sequence conservation makes it a valuable region for comparative genome evolution, phylogenetic analysis and population studies. Here, we discuss re...

journal_title：Molecular ecology resources

authors： Twyford AD,Ness RW

更新日期：2017-09-01 00:00:00

abstract：:We report the development of 10 polymorphic molecular markers containing short tandem repeats in the cosmopolitan reef-building coral, Pocillopora damicornis, an important model species for coral health, physiology, ecology, and genetics. The availability of polymorphic DNA markers in P. damicornis can act as impetus ...

journal_title：Molecular ecology resources

authors： Starger CJ,Yeoh SS,Dai CF,Baker AC,Desalle R

更新日期：2008-05-01 00:00:00

abstract：:Twelve polymorphic microsatellite loci were isolated for the bonefish, Albula vulpes using a polymerase chain reaction-based procedure. The number of alleles ranged from two to 23 (mean = 8.8) in 37 specimens from south Florida. Observed and expected heterozygosities ranged from 0.07 to 0.77 (mean = 0.42) and from 0.0...

journal_title：Molecular ecology resources

authors： Seyoum S,Wallace EM,Tringali MD

更新日期：2008-03-01 00:00:00

abstract：:QST , a measure of quantitative genetic differentiation among populations, is an index that can suggest local adaptation if QST for a trait is sufficiently larger than the mean FST of neutral genetic markers. A previous method by Whitlock and Guillaume derived a simulation resampling approach to statistically test for...

journal_title：Molecular ecology resources

authors： Gilbert KJ,Whitlock MC

更新日期：2015-03-01 00:00:00

abstract：:This article documents the whole genome sequence information of the Indian Zaprionus indianus, a member of the fruit fly family Drosophilidae. The sequences were generated on an Illumina platform and reads and whole genome sequence submitted to NCBI to the SRA and BioProject databases, respectively. This is the first ...

journal_title：Molecular ecology resources

authors： Khanna R,Mohanty S

更新日期：2017-05-01 00:00:00