Multiplex SNP-SCALE: a cost-effective medium-throughput single nucleotide polymorphism genotyping method.

abstract：:We tested for cross-species amplification of microsatellite loci located throughout the domestic sheep (Ovis aries) genome in two north American mountain ungulates (bighorn sheep, Ovis canadensis, and mountain goats, Oreamnos americanus). We identified 247 new polymorphic markers in bighorn sheep (≥ 3 alleles in one o...

journal_title：Molecular ecology resources

authors： Poissant J,Shafer AB,Davis CS,Mainguy J,Hogg JT,Côté SD,Coltman DW

更新日期：2009-07-01 00:00:00

abstract：:A microsatellite-enriched genomic library was obtained for the endangered Japanese loach Leptobotia curta, and 39 dinucleotide markers were successfully isolated and characterized. These markers had between one and nine alleles, with expected heterozygosity ranging from 0 to 0.839, in a population from the Lake Biwa-Y...

journal_title：Molecular ecology resources

authors： Watanabe K,Takeshima H,Iwata A,Abe T,Uehara K,Kakioka R,Kihira D,Nishida M

更新日期：2008-01-01 00:00:00

abstract：:The use of dominant markers such as amplified fragment length polymorphism (AFLP) for population genetics analyses is often impeded by the lack of appropriate computer programs and rarely motivated by objective considerations. The point of the present note is twofold: (i) we describe how the computer program Geneland ...

journal_title：Molecular ecology resources

authors： Guillot G,Santos F

更新日期：2010-11-01 00:00:00

abstract：:We report results of cross-species amplification in Dactylis glomerata and Lolium perenne of 12 simple sequence repeats (SSRs) isolated from Lolium multiflorum×Festuca glaucescens, 42 SSRs from Festuca arundinacea and two sequence tagged sites from Oryza sativa. We compared the transferability and diversity between D....

journal_title：Molecular ecology resources

authors： Litrico I,Bech N,Flajoulot S,Cadier D,Talon C,Gibelin C,Barre P

更新日期：2009-01-01 00:00:00

abstract：:DNA barcoding has become an important current scientific trend to the understanding of the world biodiversity. In the case of mega-diverse hot spots like Mexico, this technique represents an important tool for taxonomists, allowing them to concentrate in highlighted species by the barcodes instead of analyzing entire ...

journal_title：Molecular ecology resources

authors： Elías-Gutiérrez M,León-Regagnon V

更新日期：2013-11-01 00:00:00

abstract：:Our understanding of Glossina fuscipes fuscipes, a major vector of sleeping sickness, has been severely constrained by a lack of genetic markers for mapping and population genetic studies. Here we present 10 newly developed microsatellite loci for this tsetse species. Heterozygosity levels in Moyo, an Ugandan populati...

journal_title：Molecular ecology resources

authors： Brown JE,Komatsu KJ,Abila PP,Robinson AS,Okedi LM,Dyer N,Donnelly MJ,Slotman MA,Caccone A

更新日期：2008-11-01 00:00:00

abstract：:Publilia concava is an eastern North American membracid commonly occurring in large but spatially patchy aggregations, primarily on the host plant Solidago altissima. Like other myrmecophiles, P. concava provides sugary excretions to ants in return for the various protective, competitive or even sanitary benefits that...

journal_title：Molecular ecology resources

authors： Chhatre V,Morales MA,Abbot P

更新日期：2009-07-01 00:00:00

abstract：:Evolutionary alterations to cis-regulatory sequences are likely to cause adaptive phenotypic complexity, through orchestrating changes in cellular proliferation, identity and communication. For nonmodel organisms with adaptive key innovations, patterns of regulatory evolution have been predominantly limited to targete...

journal_title：Molecular ecology resources

authors： Kratochwil CF,Meyer A

更新日期：2015-07-01 00:00:00

abstract：:In order to facilitate the addition of a genetic component to conservation management plans for Georgia false indigo (Amorpha georgiana var. georgiana), a rare legume of the southeastern USA, 12 polymorphic microsatellite markers were developed. No gametic disequilibrium was detected among locus pairs, but observation...

journal_title：Molecular ecology resources

authors： Straub SC,Bogdanowicz SM,Doyle JJ

更新日期：2009-01-01 00:00:00

abstract：:Betel nut (Areca nut, Areca catechu L.) is a conspicuous and important cultivated plant of tropical and subtropical habitats throughout Southeast Asia and Oceania. As a significant cultural and social offering, the migration of betel nut associated with human dispersal is an important issue in ethnobotany and anthropo...

journal_title：Molecular ecology resources

authors： Hu CH,Huang CC,Hung KH,Hsu TW,Chiang TY

更新日期：2009-03-01 00:00:00

abstract：:Chimpanzee populations are diminishing as a consequence of human activities, and as a result this species is now endangered. In the context of conservation programmes, genetic data can add vital information, for instance on the genetic diversity and structure of threatened populations. Single nucleotide polymorphisms ...

journal_title：Molecular ecology resources

authors： Andrés O,Rönn AC,Bonhomme M,Kellermann T,Crouau-Roy B,Doxiadis G,Verschoor EJ,Goossens B,Domingo-Roura X,Bruford MW,Bosch M,Syvänen AC

更新日期：2008-05-01 00:00:00

abstract：:DNA-based methods have greatly enhanced the sensitivity and specificity of hematophagous arthropod bloodmeal identification. A variety of methods have been applied to study the blood-feeding behaviour of mosquitoes, ticks, black flies and other blood-feeding arthropods as it relates to host-parasite interactions and p...

journal_title：Molecular ecology resources

authors： Kent RJ

更新日期：2009-01-01 00:00:00

abstract：:Frequently, the diversity of umbrella taxa is invoked to predict patterns of other, less well-known, life. However, the utility of this strategy has been questioned. We tested whether a phylogenetic diversity (PD) analysis of CO1 DNA barcodes could act as a proxy for standard methods of determining sampling efficiency...

journal_title：Molecular ecology resources

authors： Smith MA,Fernandez-Triana J,Roughley R,Hebert PD

更新日期：2009-05-01 00:00:00

abstract：:Germplasm collections provide an extremely valuable resource for breeders and researchers. However, misclassification of accessions by species often hinders the effective use of these collections. We propose that use of high-throughput genotyping tools can provide a fast, efficient and cost-effective way of confirming...

journal_title：Molecular ecology resources

authors： Mason AS,Zhang J,Tollenaere R,Vasquez Teuber P,Dalton-Morgan J,Hu L,Yan G,Edwards D,Redden R,Batley J

更新日期：2015-09-01 00:00:00

abstract：:Fifteen polymorphic markers were developed from a microsatellite-enriched library for the lizard Podarcis gaigeae. The loci were checked for variability in 68 individuals from a population on the island of Skyros, Greece. The number of alleles ranged from 3 to 23 per locus and expected heterozygosity from 0.29 and 0.9...

journal_title：Molecular ecology resources

authors： Wellenreuther M,Runemark A,Svensson EI,Hansson B

更新日期：2009-05-01 00:00:00

abstract：:The public availability of large quantities of gene sequence data provides a valuable resource of the mining of Simple Sequence Repeat (SSR) molecular genetic markers for genetic analysis. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated gene...

journal_title：Molecular ecology resources

authors： Duran C,Singhania R,Raman H,Batley J,Edwards D

更新日期：2013-05-01 00:00:00

abstract：:As coral populations decline worldwide in the face of ongoing environmental change, documenting their distribution, diversity and conservation status is now more imperative than ever. Accurate delimitation and identification of species is a critical first step. This task, however, is not trivial as morphological varia...

journal_title：Molecular ecology resources

authors： Erickson KL,Pentico A,Quattrini AM,McFadden CS

更新日期：2021-01-01 00:00:00

abstract：:Thirty-four novel microsatellite markers developed for wood cricket (Nemobius sylvestris) were tested and optimized. Twenty-five microsatellite loci were polymorphic, exhibiting between two and nine alleles. Observed heterozygosities ranged from 0.038 to 0.925. The microsatellites were also tested in a species belongi...

journal_title：Molecular ecology resources

authors： Vanhala T,Cottrell J

更新日期：2008-11-01 00:00:00

abstract：:Bactrocera cucurbitae (Coquillett) is an important insect pest of melon fruit causing extensive losses of production in Asia and Pacific areas. Twelve polymorphic microsatellite loci were isolated from an enriched DNA library of this species and their amplification characteristics are described. Genetic parameters wer...

journal_title：Molecular ecology resources

authors： Wu Y,Li Z,Wu J

更新日期：2009-09-01 00:00:00

abstract：:We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymo...

journal_title：Molecular ecology resources

authors： Degner JF,Hether TD,Hoffman EA

更新日期：2009-03-01 00:00:00

abstract：:Single-nucleotide polymorphisms (SNPs) have potential for broad application in population and conservation genetics, but availability of these markers is limited in many nonmodel species. In this study, genomic and expressed sequence tagged (EST) sequences from closely related salmonids (Chinook salmon and rainbow tro...

journal_title：Molecular ecology resources

authors： Campbell NR,Narum SR

更新日期：2011-03-01 00:00:00

abstract：:High-throughput sequencing (HTS) is revolutionizing biological research by enabling scientists to quickly and cheaply query variation at a genomic scale. Despite the increasing ease of obtaining such data, using these data effectively still poses notable challenges, especially for those working with organisms without ...

journal_title：Molecular ecology resources

authors： Singhal S

更新日期：2013-05-01 00:00:00

abstract：:DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR o...

journal_title：Molecular ecology resources

authors： Dittrich-Schröder G,Wingfield MJ,Klein H,Slippers B

更新日期：2012-01-01 00:00:00

abstract：:Palaeoenvironments and former climates are typically inferred from pollen and macrofossil records. This approach is time-consuming and suffers from low taxonomic resolution and biased taxon sampling. Here, we test an alternative DNA-based approach utilizing the P6 loop in the chloroplast trnL (UAA) intron; a short (13...

journal_title：Molecular ecology resources

authors： Sønstebø JH,Gielly L,Brysting AK,Elven R,Edwards M,Haile J,Willerslev E,Coissac E,Rioux D,Sannier J,Taberlet P,Brochmann C

更新日期：2010-11-01 00:00:00

abstract：:We isolated 18 microsatellites from Sycoscapter australis, a nonpollinating fig wasp that develops in figs of Ficus macrophylla, and assessed their variability in 20 wasps. We further optimized nine of these loci for use in three other Sycoscapter species that develop in Ficus rubiginosa figs and assessed their variab...

journal_title：Molecular ecology resources

authors： Bouteiller-Reuter C,Hale ML,Cook JM

更新日期：2009-05-01 00:00:00

abstract：:In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heter...

journal_title：Molecular ecology resources

authors： Somta P,Musch W,Kongsamai B,Chanprame S,Nakasathien S,Toojinda T,Sorajjapinun W,Seehalak W,Tragoonrung S,Srinives P

更新日期：2008-09-01 00:00:00

abstract：:Understanding how landscape features affect gene flow has implications for numerous fields including molecular and evolutionary ecology. Despite this, modelling landscape resistance surfaces has remained a significant challenge. The R package resistancega was developed to provide a framework for optimizing landscape r...

journal_title：Molecular ecology resources

authors： Winiarski KJ,Peterman WE,McGarigal K

更新日期：2020-11-01 00:00:00

abstract：:We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

journal_title：Molecular ecology resources

authors： Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

更新日期：2008-01-01 00:00:00

abstract：:Inferring the mode and tempo of natural selection helps further our understanding of adaptation to past environmental changes. Here, we introduce McSwan, a method to detect and date past and recent natural selection events in the case of a hard sweep. The method is based on the comparison of site frequency spectra obt...

journal_title：Molecular ecology resources

authors： Tournebize R,Poncet V,Jakobsson M,Vigouroux Y,Manel S

更新日期：2019-01-01 00:00:00

abstract：:The brushtail possum is a major agricultural and ecological pest in New Zealand. A novel noninvasive DNA sampling tool for detecting its presence (WaxTags, or WT) was tested. DNA was recovered from saliva left on WT, and two lengths (407 bp and 648 bp) of the cytochrome c oxidase I (COI) barcoding region were amplifie...

journal_title：Molecular ecology resources

authors： Vargas ML,Cruickshank RH,Ross JG,Holyoake AJ,Ogilvie SC,Paterson AM

更新日期：2009-03-01 00:00:00