Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids.

abstract：:Microsatellite loci were identified from Cornus kousa'National'. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ra...

journal_title：Molecular ecology resources

authors： Wadl PA,Wang X,Scheffler BE,Rinehart TA,Trigiano RN

更新日期：2008-07-01 00:00:00

abstract：:We report an accurate multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, capable of reproducing gene expression profiles from 16 target genes [12 genes of interest (GOIs) and four reference genes (RGs)] in Acropora millepora, a common reef-building model coral species. The 12 GOIs ...

journal_title：Molecular ecology resources

authors： Souter P,Bay LK,Andreakis N,Császár N,Seneca FO,van Oppen MJ

更新日期：2011-03-01 00:00:00

abstract：:A microsatellite-enriched genomic library was developed for the water flea Daphnia atkinsoni Baird, 1859, a dominant species of intermittent wetlands in Europe. Eight polymorphic microsatellite markers were successfully optimized. Characterization of 77 individuals from Belgium and Spain showed moderate (in the former...

journal_title：Molecular ecology resources

authors： Ortells R,VAN Houdt JK,Geldof S,DE Meester L,Mergeay J

更新日期：2009-01-01 00:00:00

abstract：:The construction of microsatellite-enriched libraries is an indispensable tool to search for molecular markers as complete genome sequences are still not available for the majority of species of interest. Numerous protocols are available in the literature for the construction of these libraries; however, sometimes the...

journal_title：Molecular ecology resources

authors： Techen N,Arias RS,Glynn NC,Pan Z,Khan IA,Scheffler BE

更新日期：2010-05-01 00:00:00

abstract：:Horseshoe crabs, represented by only four extant species, have existed for around 500 million years. However, their existence is now under threat because of anthropogenic activities. The availability of genomic resources for these species will be valuable in planning appropriate conservation measures. Whole-genome seq...

journal_title：Molecular ecology resources

authors： Shingate P,Ravi V,Prasad A,Tay BH,Venkatesh B

更新日期：2020-11-01 00:00:00

abstract：:The almendro (Dipteryx panamensis, Fabaceae) is a tetraploid tree native to the Atlantic lowland rainforests of Central America. We present nine microsatellite primer pairs amplified in three multiplexed reactions for 549 individuals from four sites in Costa Rica. All loci were polymorphic, ranging from three to 13 al...

journal_title：Molecular ecology resources

authors： Hanson TR,Brunsfeld SJ,Finegan B,Waits LP

更新日期：2008-03-01 00:00:00

abstract：:We developed a total of 50 microsatellite markers for the three fungal pathogens causing the most important leaf spot diseases of banana: 32 loci for Mycosphaerella fijiensis are presented, and nine loci each for Mycosphaerella musicola and Mycosphaerella eumusae. All these loci were polymorphic within each species on...

journal_title：Molecular ecology resources

authors： Zapater MF,Duchemin M,Dussart JF,Coste D,Brottier P,Carlier J

更新日期：2008-09-01 00:00:00

abstract：:The rice stem borer, Chilo suppressalis, is one of the most damaging insect pests to rice production worldwide. Although C. suppressalis has been the focus of numerous studies examining cold tolerance and diapause, plant-insect interactions, pesticide targets and resistance, and the development of RNAi-mediated pest m...

journal_title：Molecular ecology resources

authors： Ma W,Zhao X,Yin C,Jiang F,Du X,Chen T,Zhang Q,Qiu L,Xu H,Joe Hull J,Li G,Sung WK,Li F,Lin Y

更新日期：2020-01-01 00:00:00

abstract：:Phylogenomic methods have proven useful for resolving deep nodes and recalcitrant groups in the spider tree of life. Across arachnids, transcriptomic approaches may generate thousands of loci, and target-capture methods, using the previously designed arachnid-specific probe set, can target a maximum of about 1,000 loc...

journal_title：Molecular ecology resources

authors： Kulkarni S,Wood H,Lloyd M,Hormiga G

更新日期：2020-01-01 00:00:00

abstract：:The soil microbiome is inherently complex with high biological diversity, and spatial heterogeneity typically occurring on the submillimetre scale. To study the microbial ecology of soils, and other microbiomes, biomolecules, that is, nucleic acids and proteins, must be efficiently and reliably co-recovered from the s...

journal_title：Molecular ecology resources

authors： Thorn CE,Bergesch C,Joyce A,Sambrano G,McDonnell K,Brennan F,Heyer R,Benndorf D,Abram F

更新日期：2019-03-01 00:00:00

abstract：:Crepidula convexa, a calyptreid gastropod with direct embryonic development, changes sex from male to female in the course of its lifetime (protandry). Under sex-allocation theory, male reproductive success should be independent from age and size (a proxy used for age). However, this may be counterbalanced by female c...

journal_title：Molecular ecology resources

authors： Daguin-Thiébaut C,LE Cam S,Viard F

更新日期：2009-05-01 00:00:00

abstract：:High-throughput sequencing technologies are a proposed solution for accessing the molecular data in historical specimens. However, degraded DNA combined with the computational demands of short-read assemblies has posed significant laboratory and bioinformatics challenges for de novo genome assembly. Linked-read or "sy...

journal_title：Molecular ecology resources

authors： Colella JP,Tigano A,MacManes MD

更新日期：2020-07-01 00:00:00

abstract：:Global climate change (GCC) may be causing distribution range shifts in many organisms worldwide. Multiple efforts are currently focused on the development of models to better predict distribution range shifts due to GCC. We addressed this issue by including intraspecific genetic structure and spatial autocorrelation ...

journal_title：Molecular ecology resources

authors： Martínez-Minaya J,Conesa D,Fortin MJ,Alonso-Blanco C,Picó FX,Marcer A

更新日期：2019-07-01 00:00:00

abstract：:Camelids are characterized by their unique adaptive immune system that exhibits the generation of homodimeric heavy-chain immunoglobulins, somatic hypermutation of T-cell receptors, and low genetic diversity of major histocompatibility complex (MHC) genes. However, short-read assemblies are typically highly fragmented...

journal_title：Molecular ecology resources

authors： Ming L,Wang Z,Yi L,Batmunkh M,Liu T,Siren D,He J,Juramt N,Jambl T,Li Y,Jirimutu

更新日期：2020-05-01 00:00:00

abstract：:Noninvasive genetic approaches continue to improve studies in molecular ecology, conservation genetics and related disciplines such as forensics and epidemiology. Noninvasive sampling allows genetic studies without disturbing or even seeing the target individuals. Although noninvasive genetic sampling has been used fo...

journal_title：Molecular ecology resources

authors： Beja-Pereira A,Oliveira R,Alves PC,Schwartz MK,Luikart G

更新日期：2009-09-01 00:00:00

abstract：:We have developed eight high-quality microsatellite DNA loci for the saltmarsh sharp-tailed sparrow and one additional locus with evidence of null alleles. In a sample of 250-350 individuals, the average number of alleles per locus was 14.7 and average observed heterozygosity was 0.80. These loci were tested in three ...

journal_title：Molecular ecology resources

authors： Hill CE,Tomko S,Hagen C,Schable NA,Glenn TC

更新日期：2008-01-01 00:00:00

abstract：:We combined restriction site associated DNA sequencing (RADseq) using a hypomethylation-sensitive enzyme and messenger RNA sequencing (mRNAseq) to develop molecular markers for the 16 gigabase genome of Cedrus atlantica, a conifer tree species. With each method, Illumina(®) reads from one individual were used to gener...

journal_title：Molecular ecology resources

authors： Karam MJ,Lefèvre F,Dagher-Kharrat MB,Pinosio S,Vendramin GG

更新日期：2015-05-01 00:00:00

abstract：:To characterize microbiomes and other ecological assemblages, ecologists routinely sequence and compare loci that differ among focal taxa. Counts of these sequences convey information regarding the occurrence and relative abundances of taxa, but provide no direct measure of their absolute abundances, due to the techni...

journal_title：Molecular ecology resources

authors： Harrison JG,John Calder W,Shuman B,Alex Buerkle C

更新日期：2021-01-01 00:00:00

abstract：:Long columns of migrating larval sciarid armyworms were discovered in central and northern Japan, specifically Kanagawa, Gunma, Miyagi and Akita prefectures, as well as Hokkaido. This is the first examination of armyworms in East Asia. In Europe, armyworms have been identified as Sciara militaris, belonging to the fam...

journal_title：Molecular ecology resources

authors： Sutou M,Kato T,Ito M

更新日期：2011-11-01 00:00:00

abstract：:This article documents the whole genome sequence information of the Indian Zaprionus indianus, a member of the fruit fly family Drosophilidae. The sequences were generated on an Illumina platform and reads and whole genome sequence submitted to NCBI to the SRA and BioProject databases, respectively. This is the first ...

journal_title：Molecular ecology resources

authors： Khanna R,Mohanty S

更新日期：2017-05-01 00:00:00

abstract：:We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymo...

journal_title：Molecular ecology resources

authors： Degner JF,Hether TD,Hoffman EA

更新日期：2009-03-01 00:00:00

abstract：:DNA microarrays are a popular technique for the detection of microorganisms. Several approaches using specific oligomers targeting one or a few marker genes for each species have been proposed. Data analysis is usually limited to call a species present when its oligomer exceeds a certain intensity threshold. While thi...

journal_title：Molecular ecology resources

authors： Engelmann JC,Rahmann S,Wolf M,Schultz J,Fritzilas E,Kneitz S,Dandekar T,Müller T

更新日期：2009-01-01 00:00:00

abstract：:Our understanding of Glossina fuscipes fuscipes, a major vector of sleeping sickness, has been severely constrained by a lack of genetic markers for mapping and population genetic studies. Here we present 10 newly developed microsatellite loci for this tsetse species. Heterozygosity levels in Moyo, an Ugandan populati...

journal_title：Molecular ecology resources

authors： Brown JE,Komatsu KJ,Abila PP,Robinson AS,Okedi LM,Dyer N,Donnelly MJ,Slotman MA,Caccone A

更新日期：2008-11-01 00:00:00

abstract：:The plant family Labiatae (Lamiaceae) is known for its fine medicinal and aromatic herbs like lavender, mint, oregano, sage and thyme and is a rich source of essential oils for the food, pharmaceutical and cosmetic industry. Besides its great economic importance, the Labiatae family contributes significantly to the en...

journal_title：Molecular ecology resources

authors： Theodoridis S,Stefanaki A,Tezcan M,Aki C,Kokkini S,Vlachonasios KE

更新日期：2012-07-01 00:00:00

abstract：:Predicting whether a predator is capable of affecting the dynamics of a prey species in the field implies the analysis of the complete diet of the predator, not simply rates of predation on a target taxon. Here, we employed the Ion Torrent next-generation sequencing technology to investigate the diet of a generalist a...

journal_title：Molecular ecology resources

authors： Piñol J,San Andrés V,Clare EL,Mir G,Symondson WO

更新日期：2014-01-01 00:00:00

abstract：:We introduce phylodyn, an r package for phylodynamic analysis based on gene genealogies. The package's main functionality is Bayesian nonparametric estimation of effective population size fluctuations over time. Our implementation includes several Markov chain Monte Carlo-based methods and an integrated nested Laplace...

journal_title：Molecular ecology resources

authors： Karcher MD,Palacios JA,Lan S,Minin VN

更新日期：2017-01-01 00:00:00

abstract：:This article documents the addition of 153 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brassica oleracea, Brycon amazonicus, Dimorphandra wilsonii, Eupallasella percnurus, Helleborus foetidus, Ipomoea purpurea, Phrynops geoffroanus, Prochilodus...

journal_title：Molecular ecology resources

authors： Molecular Ecology Resources Primer Development Consortium.,Aksoy S,Almeida-Val VM,Azevedo VC,Baucom R,Bazaga P,Beheregaray LB,Bennetzen JL,Brassaloti RA,Burgess TI,Caccone A,Chang SM,Ciampi AY,Ciancaleoni S,Clímaco GT,Cloue

更新日期：2013-03-01 00:00:00

abstract：:Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of...

journal_title：Molecular ecology resources

authors： Elliott CP

更新日期：2009-07-01 00:00:00

abstract：:The 'Symphyta' is a paraphyletic assemblage at the base of the order Hymenoptera, comprising 14 families and about 8750 species. All have phytophagous larvae, except for the Orussidae, which are parasitoids. This study presents and evaluates the results of DNA barcoding of approximately 5360 specimens of 'Symphyta', m...

journal_title：Molecular ecology resources

authors： Schmidt S,Taeger A,Morinière J,Liston A,Blank SM,Kramp K,Kraus M,Schmidt O,Heibo E,Prous M,Nyman T,Malm T,Stahlhut J

更新日期：2017-07-01 00:00:00

abstract：:This article documents the public availability of raw transcriptome sequence data and 63,020 SNPs for the polar bear (Ursus maritimus). ...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Coltman DW,Davis CS,Lunn NJ,Malenfant RM,Richardson ES

更新日期：2014-01-01 00:00:00