phylodyn: an R package for phylodynamic simulation and inference.

abstract：:Understanding how landscape features affect gene flow has implications for numerous fields including molecular and evolutionary ecology. Despite this, modelling landscape resistance surfaces has remained a significant challenge. The R package resistancega was developed to provide a framework for optimizing landscape r...

journal_title：Molecular ecology resources

authors： Winiarski KJ,Peterman WE,McGarigal K

更新日期：2020-11-01 00:00:00

abstract：:Here, we describe Assemble Species by Automatic Partitioning (ASAP), a new method to build species partitions from single locus sequence alignments (i.e., barcode data sets). ASAP is efficient enough to split data sets as large 104 sequences into putative species in several minutes. Although grounded in evolutionary t...

journal_title：Molecular ecology resources

authors： Puillandre N,Brouillet S,Achaz G

更新日期：2021-02-01 00:00:00

abstract：:A novel set of microsatellite markers for mungbean [Vigna radiata (L.) Wilczek] was developed from the public sequence database. Seventy-eight primers were designed and evaluated for polymorphism among 22 cultivated accessions. Eight polymorphic loci detected two to three alleles per locus with an average of 2.25. The...

journal_title：Molecular ecology resources

authors： Seehalak W,Somta P,Sommanas W,Srinives P

更新日期：2009-05-01 00:00:00

abstract：:Metabarcoding is increasingly used to assess species diversity by high-throughput sequencing where millions of sequences can be generated in parallel and multiple samples can be analysed in one sequencing run. Generating amplified fragments with a unique sequence identifier ('tag') for each sample is crucial, as it al...

journal_title：Molecular ecology resources

authors： Zizka VMA,Elbrecht V,Macher JN,Leese F

更新日期：2019-07-01 00:00:00

abstract：:The vast amount of phenotypic information collected in some wild animal populations makes them extremely valuable for unravelling the genetics of ecologically important traits and understanding how populations adapt to changes in their environment. Next generation sequencing has revolutionized the development of large...

journal_title：Molecular ecology resources

authors： Van Bers NE,Santure AW,Van Oers K,De Cauwer I,Dibbits BW,Mateman C,Crooijmans RP,Sheldon BC,Visser ME,Groenen MA,Slate J

更新日期：2012-07-01 00:00:00

abstract：:Archived specimens are highly valuable sources of DNA for retrospective genetic/genomic analysis. However, often limited effort has been made to evaluate and optimize extraction methods, which may be crucial for downstream applications. Here, we assessed and optimized the usefulness of abundant archived skeletal mater...

journal_title：Molecular ecology resources

authors： Nielsen EE,Morgan JAT,Maher SL,Edson J,Gauthier M,Pepperell J,Holmes BJ,Bennett MB,Ovenden JR

更新日期：2017-05-01 00:00:00

abstract：:The importance of assessing spatial data at multiple scales when modelling species-environment relationships has been highlighted by several empirical studies. However, no landscape genetics studies have optimized landscape resistance surfaces by evaluating relevant spatial predictors at multiple spatial scales. Here,...

journal_title：Molecular ecology resources

authors： Winiarski KJ,Peterman WE,Whiteley AR,McGarigal K

更新日期：2020-01-01 00:00:00

abstract：:The sea urchin Arbacia lixula is a keystone species in Mediterranean ecosystems that drive landscape changes in littoral communities. However, genomic information available for the whole order Arbacioida is very limited. Using RNA-seq techniques, we have characterized the transcriptome of four different tissue types i...

journal_title：Molecular ecology resources

authors： Pérez-Portela R,Turon X,Riesgo A

更新日期：2016-05-01 00:00:00

abstract：:Degraded DNA from suboptimal field sampling is common in molecular ecology. However, its impact on techniques that use restriction site associated next-generation DNA sequencing (RADSeq, GBS) is unknown. We experimentally examined the effects of in situDNA degradation on data generation for a modified double-digest RA...

journal_title：Molecular ecology resources

authors： Graham CF,Glenn TC,McArthur AG,Boreham DR,Kieran T,Lance S,Manzon RG,Martino JA,Pierson T,Rogers SM,Wilson JY,Somers CM

更新日期：2015-11-01 00:00:00

abstract：:Camelids are characterized by their unique adaptive immune system that exhibits the generation of homodimeric heavy-chain immunoglobulins, somatic hypermutation of T-cell receptors, and low genetic diversity of major histocompatibility complex (MHC) genes. However, short-read assemblies are typically highly fragmented...

journal_title：Molecular ecology resources

authors： Ming L,Wang Z,Yi L,Batmunkh M,Liu T,Siren D,He J,Juramt N,Jambl T,Li Y,Jirimutu

更新日期：2020-05-01 00:00:00

abstract：:Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...

journal_title：Molecular ecology resources

authors： Rodríguez-Ezpeleta N,Bradbury IR,Mendibil I,Álvarez P,Cotano U,Irigoien X

更新日期：2016-07-01 00:00:00

abstract：:The protist Labyrinthula zosterae (Phylum Bigyra, sensu Tsui et al. 2009) has been identified as a causative agent of wasting disease in eelgrass (Zostera marina), of which the most intense outbreak led to the destruction of 90% of eelgrass beds in eastern North America and western Europe in the 1930s. Outbreaks still...

journal_title：Molecular ecology resources

authors： Bergmann N,Fricke B,Schmidt MC,Tams V,Beining K,Schwitte H,Boettcher AA,Martin DL,Bockelmann AC,Reusch TB,Rauch G

更新日期：2011-11-01 00:00:00

abstract：:We implemented multilocus selection in a spatially-explicit, individual-based framework that enables multivariate environmental gradients to drive selection in many loci as a new module for the landscape genetics programs, CDPOP and CDMetaPOP. Our module simulates multilocus selection using a linear additive model, pr...

journal_title：Molecular ecology resources

authors： Landguth EL,Forester BR,Eckert AJ,Shirk AJ,Menon M,Whipple A,Day CC,Cushman SA

更新日期：2020-03-01 00:00:00

abstract：:The brushtail possum is a major agricultural and ecological pest in New Zealand. A novel noninvasive DNA sampling tool for detecting its presence (WaxTags, or WT) was tested. DNA was recovered from saliva left on WT, and two lengths (407 bp and 648 bp) of the cytochrome c oxidase I (COI) barcoding region were amplifie...

journal_title：Molecular ecology resources

authors： Vargas ML,Cruickshank RH,Ross JG,Holyoake AJ,Ogilvie SC,Paterson AM

更新日期：2009-03-01 00:00:00

abstract：:Quantitative real-time PCR (qPCR) is slowly becoming established as a tool to quantify abundance of different arbuscular mycorrhizal fungal (AMF) taxa in roots and in soil. Here, we describe the development and field validation of qPCR markers (i.e. primers with associated hydrolysis probes), targeting taxon-specific ...

journal_title：Molecular ecology resources

authors： Thonar C,Erb A,Jansa J

更新日期：2012-03-01 00:00:00

abstract：:We developed 11 polymorphic microsatellite DNA markers for an Australian native plant, Daviesia triflora. The number of alleles per locus in 40 individuals varied from four to 19, and observed and expected heterozygosities ranged from 0.450 to 0.925 and from 0.497 to 0.899, respectively. Nine loci showed no significan...

journal_title：Molecular ecology resources

authors： He T,Krauss SL,Lamont BB

更新日期：2008-11-01 00:00:00

abstract：:Most hosts are concurrently or sequentially infected with multiple parasites; thus, fully understanding interactions between individual parasite species and their hosts depends on accurate characterization of the parasite community. For parasitic nematodes, noninvasive methods for obtaining quantitative, species-speci...

journal_title：Molecular ecology resources

authors： Budischak SA,Hoberg EP,Abrams A,Jolles AE,Ezenwa VO

更新日期：2015-09-01 00:00:00

abstract：:Aleppo pine (Pinus halepensis Mill.) is a relevant conifer species for studying adaptive responses to drought and fire regimes in the Mediterranean region. In this study, we performed Illumina next-generation sequencing of two phenotypically divergent Aleppo pine accessions with the aims of (i) characterizing the tran...

journal_title：Molecular ecology resources

authors： Pinosio S,González-Martínez SC,Bagnoli F,Cattonaro F,Grivet D,Marroni F,Lorenzo Z,Pausas JG,Verdú M,Vendramin GG

更新日期：2014-07-01 00:00:00

abstract：:Our study evaluated in silico the potential of 14 mitochondrial genes encoding the subunits of the respiratory chain complexes, including cytochrome c oxidase I (CO1), as Basidiomycota DNA barcode. Fifteen complete and partial mitochondrial genomes were recovered and characterized in this study. Mitochondrial genes sh...

journal_title：Molecular ecology resources

authors： Vialle A,Feau N,Allaire M,Didukh M,Martin F,Moncalvo JM,Hamelin RC

更新日期：2009-05-01 00:00:00

abstract：:Local adaptation is often studied via (i) multiple common garden experiments comparing performance of genotypes in different environments and (ii) sequencing genotypes from multiple locations and characterizing geographic patterns in allele frequency. Both approaches aim to characterize the same pattern (local adaptat...

journal_title：Molecular ecology resources

authors： Lasky JR,Forester BR,Reimherr M

更新日期：2018-01-01 00:00:00

abstract：:A genomic library was constructed and 13 polymorphic microsatellite markers were developed for Hakea oldfieldii, a woody shrub endemic to southwest Western Australia. Polymorphism was investigated for these markers in 28 individuals from a single population located in restricted habitat at the base of the Whicher Rang...

journal_title：Molecular ecology resources

authors： Byrne M,Hankinson M

更新日期：2009-07-01 00:00:00

abstract：:For nonmodel organisms, genome-wide information that describes functionally relevant variation may be obtained by RNA-Seq following de novo transcriptome assembly. While sequencing has become relatively inexpensive, the preparation of a large number of sequencing libraries remains prohibitively expensive for populatio...

journal_title：Molecular ecology resources

authors： Konczal M,Koteja P,Stuglik MT,Radwan J,Babik W

更新日期：2014-03-01 00:00:00

abstract：:DNA sourced from faeces is notoriously less reliable than that from tissue. Hence, understanding whether faecal pellet quality varies within faecal piles may be important for sample selection. We hypothesized that the order in which faecal pellets are deposited may influence microsatellite polymerase chain reaction (P...

journal_title：Molecular ecology resources

authors： Walker FM,Horsup A,Taylor AC

更新日期：2009-05-01 00:00:00

abstract：:Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alle...

journal_title：Molecular ecology resources

authors： Anderson SJ,Stone CL,Posada-Buitrago ML,Boore JL,Neelam BA,Stephens RM,Luster DG,Frederick RD,Pedley KF

更新日期：2008-11-01 00:00:00

abstract：:We report the development of 10 polymorphic molecular markers containing short tandem repeats in the cosmopolitan reef-building coral, Pocillopora damicornis, an important model species for coral health, physiology, ecology, and genetics. The availability of polymorphic DNA markers in P. damicornis can act as impetus ...

journal_title：Molecular ecology resources

authors： Starger CJ,Yeoh SS,Dai CF,Baker AC,Desalle R

更新日期：2008-05-01 00:00:00

abstract：:The construction of microsatellite-enriched libraries is an indispensable tool to search for molecular markers as complete genome sequences are still not available for the majority of species of interest. Numerous protocols are available in the literature for the construction of these libraries; however, sometimes the...

journal_title：Molecular ecology resources

authors： Techen N,Arias RS,Glynn NC,Pan Z,Khan IA,Scheffler BE

更新日期：2010-05-01 00:00:00

abstract：:DNA barcoding is a molecular method that rapidly identifies an individual to a known taxon or its closest relative based on a 650-bp fragment of the cytochrome c oxidase subunit I (COI). In this study, DNA barcodes of members of the family Accipitridae, including Haliastur indus (brahminy kite), Haliaeetus leucogaster...

journal_title：Molecular ecology resources

authors： Ong PS,Luczon AU,Quilang JP,Sumaya AM,Ibañez JC,Salvador DJ,Fontanilla IK

更新日期：2011-03-01 00:00:00

abstract：:Eight polymorphic microsatellite loci were isolated and characterized from a microsatellite DNA-enriched DNA library for the leopard coralgrouper (Plectropomus leopardus Lacepède, 1802), a popular food fish in the East Indies. These loci showed polymorphism information content ranging from 0.493 to 0.854, allele numbe...

journal_title：Molecular ecology resources

authors： Ding SX,Zeng HS,Wang Y,Pan Y,Shi XF

更新日期：2009-11-01 00:00:00

abstract：:We isolated 18 microsatellites from Sycoscapter australis, a nonpollinating fig wasp that develops in figs of Ficus macrophylla, and assessed their variability in 20 wasps. We further optimized nine of these loci for use in three other Sycoscapter species that develop in Ficus rubiginosa figs and assessed their variab...

journal_title：Molecular ecology resources

authors： Bouteiller-Reuter C,Hale ML,Cook JM

更新日期：2009-05-01 00:00:00

abstract：:In order to facilitate the addition of a genetic component to conservation management plans for Georgia false indigo (Amorpha georgiana var. georgiana), a rare legume of the southeastern USA, 12 polymorphic microsatellite markers were developed. No gametic disequilibrium was detected among locus pairs, but observation...

journal_title：Molecular ecology resources

authors： Straub SC,Bogdanowicz SM,Doyle JJ

更新日期：2009-01-01 00:00:00