ASAP: assemble species by automatic partitioning.

Abstract:

:Here, we describe Assemble Species by Automatic Partitioning (ASAP), a new method to build species partitions from single locus sequence alignments (i.e., barcode data sets). ASAP is efficient enough to split data sets as large 104 sequences into putative species in several minutes. Although grounded in evolutionary theory, ASAP is the implementation of a hierarchical clustering algorithm that only uses pairwise genetic distances, avoiding the computational burden of phylogenetic reconstruction. Importantly, ASAP proposes species partitions ranked by a new scoring system that uses no biological prior insight of intraspecific diversity. ASAP is a stand-alone program that can be used either through a graphical web-interface or that can be downloaded and compiled for local usage. We have assessed its power along with three others programs (ABGD, PTP and GMYC) on 10 real COI barcode data sets representing various degrees of challenge (from small and easy cases to large and complicated data sets). We also used Monte-Carlo simulations of a multispecies coalescent framework to assess the strengths and weaknesses of ASAP and the other programs. Through these analyses, we demonstrate that ASAP has the potential to become a major tool for taxonomists as it proposes rapidly in a full graphical exploratory interface relevant species hypothesis as a first step of the integrative taxonomy process.

journal_name

Mol Ecol Resour

authors

Puillandre N,Brouillet S,Achaz G

doi

10.1111/1755-0998.13281

subject

Has Abstract

pub_date

2021-02-01 00:00:00

pages

609-620

issue

2

eissn

1755-098X

issn

1755-0998

journal_volume

21

pub_type

杂志文章
  • Streamlining universal single-copy orthologue and ultraconserved element design: A case study in Collembola.

    abstract::Genomic data sets are increasingly central to ecological and evolutionary biology, but far fewer resources are available for invertebrates. Powerful new computational tools and the rapidly decreasing cost of Illumina sequencing are beginning to change this, enabling rapid genome assembly and reference marker extractio...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13146

    authors: Sun X,Ding Y,Orr MC,Zhang F

    更新日期:2020-05-01 00:00:00

  • Genomic resources notes accepted 1 August 2013-30 September 2013.

    abstract::This article documents the public availability of raw transcriptome sequence data and 63,020 SNPs for the polar bear (Ursus maritimus). ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12190

    authors: Genomic Resources Development Consortium.,Coltman DW,Davis CS,Lunn NJ,Malenfant RM,Richardson ES

    更新日期:2014-01-01 00:00:00

  • A combined parasitological molecular approach for noninvasive characterization of parasitic nematode communities in wild hosts.

    abstract::Most hosts are concurrently or sequentially infected with multiple parasites; thus, fully understanding interactions between individual parasite species and their hosts depends on accurate characterization of the parasite community. For parasitic nematodes, noninvasive methods for obtaining quantitative, species-speci...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12382

    authors: Budischak SA,Hoberg EP,Abrams A,Jolles AE,Ezenwa VO

    更新日期:2015-09-01 00:00:00

  • Development of 54 novel single-nucleotide polymorphism (SNP) assays for sockeye and coho salmon and assessment of available SNPs to differentiate stocks within the Columbia River.

    abstract::Single-nucleotide polymorphisms (SNPs) have potential for broad application in population and conservation genetics, but availability of these markers is limited in many nonmodel species. In this study, genomic and expressed sequence tagged (EST) sequences from closely related salmonids (Chinook salmon and rainbow tro...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.02977.x

    authors: Campbell NR,Narum SR

    更新日期:2011-03-01 00:00:00

  • The first transcriptome and genetic linkage map for Asian arowana.

    abstract::Asian arowana or dragonfish (Scleropages formosus) is an important fish species due to its unusual breeding biology and high economic value in the ornamental fish markets. In the present study, we aimed to (i) create the first transcriptome by Roche 454 pyrosequencing of Asian arowana brain and gonad samples; (ii) ide...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12212

    authors: Shen XY,Kwan HY,Thevasagayam NM,Prakki SR,Kuznetsova IS,Ngoh SY,Lim Z,Feng F,Chang A,Orbán L

    更新日期:2014-05-01 00:00:00

  • Evaluation of the R package 'resistancega': A promising approach towards the accurate optimization of landscape resistance surfaces.

    abstract::Understanding how landscape features affect gene flow has implications for numerous fields including molecular and evolutionary ecology. Despite this, modelling landscape resistance surfaces has remained a significant challenge. The R package resistancega was developed to provide a framework for optimizing landscape r...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13217

    authors: Winiarski KJ,Peterman WE,McGarigal K

    更新日期:2020-11-01 00:00:00

  • The chromosome-level genome sequence and karyotypic evolution of Megadenia pygmaea (Brassicaceae).

    abstract::Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was a...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13291

    authors: Yang W,Zhang L,Mandáková T,Huang L,Li T,Jiang J,Yang Y,Lysak MA,Liu J,Hu Q

    更新日期:2020-11-05 00:00:00

  • Genomic sequence capture of haemosporidian parasites: Methods and prospects for enhanced study of host-parasite evolution.

    abstract::Avian malaria and related haemosporidians (Plasmodium, [Para]Haemoproteus and Leucocytoozoon) represent an exciting multihost, multiparasite system in ecology and evolution. Global research in this field accelerated after the publication in 2000 of PCR protocols to sequence a haemosporidian mitochondrial (mtDNA) barco...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12977

    authors: Barrow LN,Allen JM,Huang X,Bensch S,Witt CC

    更新日期:2019-03-01 00:00:00

  • ednaoccupancy: An r package for multiscale occupancy modelling of environmental DNA data.

    abstract::In this article, we describe ednaoccupancy, an r package for fitting Bayesian, multiscale occupancy models. These models are appropriate for occupancy surveys that include three nested levels of sampling: primary sample units within a study area, secondary sample units collected from each primary unit and replicates o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12735

    authors: Dorazio RM,Erickson RA

    更新日期:2018-03-01 00:00:00

  • An objective, rapid and reproducible method for scoring AFLP peak-height data that minimizes genotyping error.

    abstract::Amplified fragment length polymorphism (AFLP) fingerprint data are now commonly collected using DNA sequencers. AFLP genotypes are still often scored by eye from such data - a time-consuming, error-prone and subjective process. We present a semi-automated method of genotyping sequencer-collected AFLPs at predefined fr...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2007.02073.x

    authors: Whitlock R,Hipperson H,Mannarelli M,Butlin RK,Burke T

    更新日期:2008-07-01 00:00:00

  • Mapping active promoters by ChIP-seq profiling of H3K4me3 in cichlid fish - a first step to uncover cis-regulatory elements in ecological model teleosts.

    abstract::Evolutionary alterations to cis-regulatory sequences are likely to cause adaptive phenotypic complexity, through orchestrating changes in cellular proliferation, identity and communication. For nonmodel organisms with adaptive key innovations, patterns of regulatory evolution have been predominantly limited to targete...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12350

    authors: Kratochwil CF,Meyer A

    更新日期:2015-07-01 00:00:00

  • Development of microsatellite markers in the St Lucia anole, Anolis luciae.

    abstract::Anolis lizards are important models in studies of ecology and evolution. Here we describe 13 polymorphic microsatellites for use in population screening in the St Lucia anole, Anolis luciae, that can be used as a natural replicate to Anolis roquet on Martinique to study processes involved in population differentiation...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02206.x

    authors: Johansson H,Surget-Groba Y,Gow JL,Thorpe RS

    更新日期:2008-11-01 00:00:00

  • DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps.

    abstract::DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.03074.x

    authors: Dittrich-Schröder G,Wingfield MJ,Klein H,Slippers B

    更新日期:2012-01-01 00:00:00

  • McSwan: A joint site frequency spectrum method to detect and date selective sweeps across multiple population genomes.

    abstract::Inferring the mode and tempo of natural selection helps further our understanding of adaptation to past environmental changes. Here, we introduce McSwan, a method to detect and date past and recent natural selection events in the case of a hard sweep. The method is based on the comparison of site frequency spectra obt...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12957

    authors: Tournebize R,Poncet V,Jakobsson M,Vigouroux Y,Manel S

    更新日期:2019-01-01 00:00:00

  • Advancing nematode barcoding: a primer cocktail for the cytochrome c oxidase subunit I gene from vertebrate parasitic nematodes.

    abstract::Although nematodes are one of the most diverse metazoan phyla, species identification through morphology is difficult. Several genetic markers have been used for their identification, but most do not provide species-level resolution in all groups, and those that do lack primer sets effective across the phylum, preclud...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12082

    authors: Prosser SW,Velarde-Aguilar MG,León-Règagnon V,Hebert PD

    更新日期:2013-11-01 00:00:00

  • A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus.

    abstract::To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of gen...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02931.x

    authors: Murata C,Ogura G,Kuroiwa A

    更新日期:2011-03-01 00:00:00

  • A newly developed genetic sex marker and its application to understanding chemically induced feminisation in roach (Rutilus rutilus).

    abstract::Oestrogenic wastewater treatment works (WwTW) effluents discharged into UK rivers have been shown to affect sexual development, including inducing intersex, in wild roach (Rutilus rutilus). This can result in a reduced breeding capability with potential population level impacts. In the absence of a sex probe for roach...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13166

    authors: Lange A,Paris JR,Gharbi K,Cézard T,Miyagawa S,Iguchi T,Studholme DJ,Tyler CR

    更新日期:2020-07-01 00:00:00

  • Sequenced RAPD markers to detect hybridization in the barbary partridge (Alectoris barbara, Phasianidae).

    abstract::In the Alectoris partridges (Phasianidae), hybridization occurs occasionally as a result of the natural breakdown of isolating mechanisms but more frequently as a result of human activity. No genetic record of hybridization is known for the barbary partridge (A. barbara). This species is distributed mostly in North Af...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02880.x

    authors: Barbanera F,Guerrini M,Bertoncini F,Cappelli F,Muzzeddu M,Dini F

    更新日期:2011-01-01 00:00:00

  • Exon probe sets and bioinformatics pipelines for all levels of fish phylogenomics.

    abstract::Exon markers have a long history of use in phylogenetics of ray-finned fishes, the most diverse clade of vertebrates with more than 35,000 species. As the number of published genomes increases, it has become easier to test exons and other genetic markers for signals of ancient duplication events and filter out paralog...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13287

    authors: Hughes LC,Ortí G,Saad H,Li C,White WT,Baldwin CC,Crandall KA,Arcila D,Betancur-R R

    更新日期:2020-10-21 00:00:00

  • Evaluation of primer pairs for microbiome profiling from soils to humans within the One Health framework.

    abstract::The 'One Health' framework emphasizes the ecological relationships between soil, plant, animal and human health. Microbiomes play important roles in these relationships, as they modify the health and performance of the different compartments and influence the transfer of energy, matter and chemicals between them. Stan...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13215

    authors: Wasimuddin,Schlaeppi K,Ronchi F,Leib SL,Erb M,Ramette A

    更新日期:2020-11-01 00:00:00

  • High-throughput microsatellite genotyping in ecology: improved accuracy, efficiency, standardization and success with low-quantity and degraded DNA.

    abstract::Microsatellite markers have played a major role in ecological, evolutionary and conservation research during the past 20 years. However, technical constrains related to the use of capillary electrophoresis and a recent technological revolution that has impacted other marker types have brought to question the continued...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12594

    authors: De Barba M,Miquel C,Lobréaux S,Quenette PY,Swenson JE,Taberlet P

    更新日期:2017-05-01 00:00:00

  • Identification of Culex complex species using SNP markers based on high-resolution melting analysis.

    abstract::Mosquitoes belonging to the Culex pipiens complex are primary vectors for diseases such as West Nile encephalitis, Eastern equine encephalitis, many arboviruses, as well as lymphatic filariases. Despite sharing physiological characteristics, each mosquito species within the Culex complex has unique behavioural and rep...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12083

    authors: Kang D,Sim C

    更新日期:2013-05-01 00:00:00

  • Characterization of microsatellite markers for Sycoscapter nonpollinating fig wasps.

    abstract::We isolated 18 microsatellites from Sycoscapter australis, a nonpollinating fig wasp that develops in figs of Ficus macrophylla, and assessed their variability in 20 wasps. We further optimized nine of these loci for use in three other Sycoscapter species that develop in Ficus rubiginosa figs and assessed their variab...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02284.x

    authors: Bouteiller-Reuter C,Hale ML,Cook JM

    更新日期:2009-05-01 00:00:00

  • Molecular methods for arthropod bloodmeal identification and applications to ecological and vector-borne disease studies.

    abstract::DNA-based methods have greatly enhanced the sensitivity and specificity of hematophagous arthropod bloodmeal identification. A variety of methods have been applied to study the blood-feeding behaviour of mosquitoes, ticks, black flies and other blood-feeding arthropods as it relates to host-parasite interactions and p...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02469.x

    authors: Kent RJ

    更新日期:2009-01-01 00:00:00

  • A multilocus, temperature stress-related gene expression profile assay in Acropora millepora, a dominant reef-building coral.

    abstract::We report an accurate multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, capable of reproducing gene expression profiles from 16 target genes [12 genes of interest (GOIs) and four reference genes (RGs)] in Acropora millepora, a common reef-building model coral species. The 12 GOIs ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02923.x

    authors: Souter P,Bay LK,Andreakis N,Császár N,Seneca FO,van Oppen MJ

    更新日期:2011-03-01 00:00:00

  • A factorial design experiment as a pilot study for noninvasive genetic sampling.

    abstract::Noninvasive genetic sampling has increasingly been used in ecological and conservation studies during the last decade. A major part of the noninvasive genetic literature is dedicated to the search for optimal protocols, by comparing different methods of collection, preservation and extraction of DNA from noninvasive m...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2012.03170.x

    authors: Renan S,Speyer E,Shahar N,Gueta T,Templeton AR,Bar-David S

    更新日期:2012-11-01 00:00:00

  • PERMANENT GENETIC RESOURCES: Isolation of 60 polymorphic microsatellite loci in EST libraries of four sibling species of the phytopathogenic fungal complex Microbotryum.

    abstract::We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum, causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis-dioicae, M. violaceum sensus stricto, M. lagerheimii and M....

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.01967.x

    authors: Giraud T,Yockteng R,Marthey S,Chiapello H,Jonot O,Lopez-Villavicencio M,DE Vienne DM,Hood ME,Refregier G,Gendrault-Jacquemard A,Wincker P,Dossat C

    更新日期:2008-03-01 00:00:00

  • Assessing the influence of sample tagging and library preparation on DNA metabarcoding.

    abstract::Metabarcoding is increasingly used to assess species diversity by high-throughput sequencing where millions of sequences can be generated in parallel and multiple samples can be analysed in one sequencing run. Generating amplified fragments with a unique sequence identifier ('tag') for each sample is crucial, as it al...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13018

    authors: Zizka VMA,Elbrecht V,Macher JN,Leese F

    更新日期:2019-07-01 00:00:00

  • Characterization of polymorphic microsatellite DNA markers in Hakea oldfieldii Benth. (Proteaceae).

    abstract::A genomic library was constructed and 13 polymorphic microsatellite markers were developed for Hakea oldfieldii, a woody shrub endemic to southwest Western Australia. Polymorphism was investigated for these markers in 28 individuals from a single population located in restricted habitat at the base of the Whicher Rang...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02616.x

    authors: Byrne M,Hankinson M

    更新日期:2009-07-01 00:00:00

  • Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids.

    abstract::We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.01917.x

    authors: Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

    更新日期:2008-01-01 00:00:00