Abstract:
:To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of genomic DNA tested: males yielded two bands and females a single band. Sequencing of each PCR product confirmed that the 769-bp fragment amplified from DNA samples of both sexes was derived from EIF2S3X, whereas the 546-bp fragment amplified only from male DNA samples was derived from EIF2S3Y. The results indicated that this primer set is useful for sex identification in this species.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Murata C,Ogura G,Kuroiwa Adoi
10.1111/j.1755-0998.2010.02931.xsubject
Has Abstractpub_date
2011-03-01 00:00:00pages
386-8issue
2eissn
1755-098Xissn
1755-0998journal_volume
11pub_type
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