Forensic identification of the guitarfish species Rhinobatos horkelli, R. percellens and Zapteryx brevirostris using multiplex-PCR.

Abstract:

:Rhinobatos percellens, Rhinobatos horkelli and Zapteryx brevirostris, known as guitarfish, are commonly found inshore and caught by trawl nets. Recently, these species have been suffering sharp population declines. One of the main impediments to the development of conservation plans is the lack of information related to each species catch. Gathering this information is made more difficult by the fishermen's practice of removing parts of the animals before landing, as well as the morphological similarities among the three species. To address these issues, this work presents multiplex-PCR protocols that were developed to distinguish among these species using their genetic characteristics.

journal_name

Mol Ecol Resour

authors

DE-Franco B,Mendonça FF,Hashimoto DT,Porto-Foresti F,Oliveira C,Foresti F

doi

10.1111/j.1755-0998.2009.02728.x

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

197-9

issue

1

eissn

1755-098X

issn

1755-0998

journal_volume

10

pub_type

杂志文章
  • Ten new microsatellite markers for the buttonwood mangrove (Conocarpus erectus L., Combretaceae).

    abstract::We present 10 microsatellite markers for the buttonwood mangrove, Conocarpus erectus, a wide-range mangrove associate species. Polymorphism was assessed among individuals from six different populations along the Pacific Coast of Mexico and Costa Rica, as well as in two individuals from the Yucatan Peninsula in the Atl...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02088.x

    authors: Nettel A,Dodd RS,Cid-Becerra JA,DE LA Rosa-Velez J

    更新日期:2008-07-01 00:00:00

  • Simple identification of mitochondrial lineages in contact zones based on lineage-selective primers.

    abstract::A variety of research projects focus on genetic variation among and within maternal lineages as encompassed by mitochondrial DNA (mtDNA). While mtDNA often differs substantially between species, large differences may also be found within species. The evaluation of such divergent lineages, for example in intraspecific ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.01894.x

    authors: Lindell J,Murphy RW

    更新日期:2008-01-01 00:00:00

  • Applying DNA barcoding for the study of geographical variation in host-parasitoid interactions.

    abstract::Studies on the biogeography of host-parasitoid interactions are scarce, mainly because of technical difficulties associated with rearing and species identification. DNA barcoding is increasingly recognized as a valuable tool for taxon identification, allowing to link different life history stages of a species. We eval...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02889.x

    authors: Santos AM,Besnard G,Quicke DL

    更新日期:2011-01-01 00:00:00

  • Isolation of compound microsatellite loci in the herbaceous perennial Cirsium purpuratum (Maxim.) Matsum.

    abstract::The herbaceous perennial Cirsium purpuratum is a pioneer on the southeast side of Mount Fuji in Japan. For genetic analysis of reproduction in this species, we developed polymorphic compound microsatellite markers using an adaptor-ligated library method and a simpler method called the intercompound microsatellite meth...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2007.02077.x

    authors: Wu B,Shiokawa A,Matsushita N,Hogetsu T

    更新日期:2008-07-01 00:00:00

  • Isolation and characterization of eight microsatellite loci from the longheaded eagle ray, Aetobatus flagellum (Elasmobranchii, Myliobatidae).

    abstract::The longheaded eagle ray, Aetobatus flagellum feeds mainly on bivalves, and there have been some indications that the current reduction in the bivalve population in southern Japan may be due to an increase in the number of longheaded eagle rays. Consequently, the 'predator control programme' for reducing the longheade...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02568.x

    authors: Yagishita N,Yamaguchi A

    更新日期:2009-05-01 00:00:00

  • Microsatellites from kousa dogwood (Cornus kousa).

    abstract::Microsatellite loci were identified from Cornus kousa'National'. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ra...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2007.02062.x

    authors: Wadl PA,Wang X,Scheffler BE,Rinehart TA,Trigiano RN

    更新日期:2008-07-01 00:00:00

  • Assessing the influence of sample tagging and library preparation on DNA metabarcoding.

    abstract::Metabarcoding is increasingly used to assess species diversity by high-throughput sequencing where millions of sequences can be generated in parallel and multiple samples can be analysed in one sequencing run. Generating amplified fragments with a unique sequence identifier ('tag') for each sample is crucial, as it al...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13018

    authors: Zizka VMA,Elbrecht V,Macher JN,Leese F

    更新日期:2019-07-01 00:00:00

  • Applicability of RAD-tag genotyping for interfamilial comparisons: empirical data from two cetaceans.

    abstract::Restriction-site-associated DNA tag (RAD-tag) sequencing has become a popular approach to generate thousands of SNPs used to address diverse questions in population genomics. Comparatively, the suitability of RAD-tag genotyping to address evolutionary questions across divergent species has been the subject of only a f...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12206

    authors: Viricel A,Pante E,Dabin W,Simon-Bouhet B

    更新日期:2014-05-01 00:00:00

  • Isolation and characterization of microsatellites in the bird-pollinated, autohexaploid, Eremophila glabra ssp. glabra (R.Br. (Ostenf.)) (Myoporaceae), an Australian endemic plant.

    abstract::Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02618.x

    authors: Elliott CP

    更新日期:2009-07-01 00:00:00

  • Rapid identification of thousands of copperhead snake (Agkistrodon contortrix) microsatellite loci from modest amounts of 454 shotgun genome sequence.

    abstract::Optimal integration of next-generation sequencing into mainstream research requires re-evaluation of how problems can be reasonably overcome and what questions can be asked. One potential application is the rapid acquisition of genomic information to identify microsatellite loci for evolutionary, population genetic an...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02750.x

    authors: Castoe TA,Poole AW,Gu W,Jason de Koning AP,Daza JM,Smith EN,Pollock DD

    更新日期:2010-03-01 00:00:00

  • Microsatellites from the charcoal rot fungus (Macrophomina phaseolina).

    abstract::Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were p...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02511.x

    authors: Baird RE,Wadl PA,Wang X,Johnson DH,Rinehart TA,Abbas HK,Shier T,Trigiano RN

    更新日期:2009-05-01 00:00:00

  • Expressed sequence tag-simple sequence repeats isolated from Shorea leprosula and their transferability to 36 species within the Dipterocarpaceae.

    abstract::Simple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) are valuable markers because they represent transcribed regions and often transferable to related taxa. Here, we report the development and characterization of EST-SSRs from Shorea leprosula. Fifty-four sequences containing SSRs were identified...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02238.x

    authors: Ng KK,Lee SL,Tsumura Y,Ueno S,Ng CH,Lee CT

    更新日期:2009-01-01 00:00:00

  • Isolation and characterization of polymorphic microsatellite loci for the Skyros wall lizard Podarcis gaigeae (Squamata: Lacertidae).

    abstract::Fifteen polymorphic markers were developed from a microsatellite-enriched library for the lizard Podarcis gaigeae. The loci were checked for variability in 68 individuals from a population on the island of Skyros, Greece. The number of alleles ranged from 3 to 23 per locus and expected heterozygosity from 0.29 and 0.9...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02550.x

    authors: Wellenreuther M,Runemark A,Svensson EI,Hansson B

    更新日期:2009-05-01 00:00:00

  • A robust, cost-effective method for DNA, RNA and protein co-extraction from soil, other complex microbiomes and pure cultures.

    abstract::The soil microbiome is inherently complex with high biological diversity, and spatial heterogeneity typically occurring on the submillimetre scale. To study the microbial ecology of soils, and other microbiomes, biomolecules, that is, nucleic acids and proteins, must be efficiently and reliably co-recovered from the s...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12979

    authors: Thorn CE,Bergesch C,Joyce A,Sambrano G,McDonnell K,Brennan F,Heyer R,Benndorf D,Abram F

    更新日期:2019-03-01 00:00:00

  • Streamlining universal single-copy orthologue and ultraconserved element design: A case study in Collembola.

    abstract::Genomic data sets are increasingly central to ecological and evolutionary biology, but far fewer resources are available for invertebrates. Powerful new computational tools and the rapidly decreasing cost of Illumina sequencing are beginning to change this, enabling rapid genome assembly and reference marker extractio...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13146

    authors: Sun X,Ding Y,Orr MC,Zhang F

    更新日期:2020-05-01 00:00:00

  • Development of nine new microsatellite loci for the American beaver, Castor canadensis (Rodentia: Castoridae), and cross-species amplification in the European beaver, Castor fiber.

    abstract::We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cr...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02364.x

    authors: Pelz-Serrano K,Munguia-Vega A,Piaggio AJ,Neubaum M,Munclinger P,Pártl A,VAN Riper Iii C,Culver M

    更新日期:2009-03-01 00:00:00

  • AMaCAID: a useful tool for Accurate Marker Choice for Accession Identification and Discrimination.

    abstract::AMaCAID is an R program designed to analyse multilocus genotypic patterns in large samples. It allows (i) the computation of the number and frequency of the different multilocus patterns available in a molecular data set and (ii) the analysis of discriminatory power of each combination of k markers among n available. ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.02993.x

    authors: Caroli S,Santoni S,Ronfort J

    更新日期:2011-07-01 00:00:00

  • Ten di- and trinucleotide microsatellite loci in the Caribbean spiny lobster, Panulirus argus, for studies of regional population connectivity.

    abstract::We describe 10 microsatellite loci for Panulirus argus (Caribbean spiny lobster). The number of alleles at each locus ranged from four to 39 (mean = 21.8) in 89 juvenile specimens collected at two different times at a recruitment site in south Florida. Levels of expected and observed heterozygosities ranged from 0.48 ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.02032.x

    authors: Tringali MD,Seyoum S,Schmitt SL

    更新日期:2008-05-01 00:00:00

  • A chromosome-level genome assembly of rice leaffolder, Cnaphalocrocis medinalis.

    abstract::The rice leaffolder, Cnaphalocrocis medinalis Guenée (Crambidae, Lepidoptera), is an important agricultural pest that causes serious losses to rice production in rice-growing regions with high humidity and temperature. However, a lack of genomic resources limits in-depth understanding of its biological characteristics...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13274

    authors: Zhao X,Xu H,He K,Shi Z,Chen X,Ye X,Mei Y,Yang Y,Li M,Gao L,Xu L,Xiao H,Liu Y,Lu Z,Li F

    更新日期:2021-02-01 00:00:00

  • Evaluating environmental DNA-based quantification of ranavirus infection in wood frog populations.

    abstract::A variety of challenges arise when monitoring wildlife populations for disease. Sampling tissues can be invasive to hosts, and obtaining sufficient sample sizes can be expensive and time-consuming, particularly for rare species and when pathogen prevalence is low. Environmental DNA (eDNA)-based detection of pathogens ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12461

    authors: Hall EM,Crespi EJ,Goldberg CS,Brunner JL

    更新日期:2016-03-01 00:00:00

  • A combined parasitological molecular approach for noninvasive characterization of parasitic nematode communities in wild hosts.

    abstract::Most hosts are concurrently or sequentially infected with multiple parasites; thus, fully understanding interactions between individual parasite species and their hosts depends on accurate characterization of the parasite community. For parasitic nematodes, noninvasive methods for obtaining quantitative, species-speci...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12382

    authors: Budischak SA,Hoberg EP,Abrams A,Jolles AE,Ezenwa VO

    更新日期:2015-09-01 00:00:00

  • Assessment of loci for DNA barcoding in the genus Thrips (Thysanoptera:Thripidae).

    abstract::Identification of the juveniles of economically important thrips species on imports by morphology alone can be challenging and culturing is usually required. In the case of EU quarantine species such as Thrips palmi, rapid and accurate identification is essential. DNA barcoding using the Cytochrome oxidase I (COI) gen...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02723.x

    authors: Glover RH,Collins DW,Walsh K,Boonham N

    更新日期:2010-01-01 00:00:00

  • DNA metabarcoding for biodiversity monitoring in a national park: Screening for invasive and pest species.

    abstract::DNA metabarcoding was utilized for a large-scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count-based biodiversities revealed clustering in concordance with whether collection sites were located ins...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13212

    authors: Hardulak LA,Morinière J,Hausmann A,Hendrich L,Schmidt S,Doczkal D,Müller J,Hebert PDN,Haszprunar G

    更新日期:2020-11-01 00:00:00

  • Multiscale resistant kernel surfaces derived from inferred gene flow: An application with vernal pool breeding salamanders.

    abstract::The importance of assessing spatial data at multiple scales when modelling species-environment relationships has been highlighted by several empirical studies. However, no landscape genetics studies have optimized landscape resistance surfaces by evaluating relevant spatial predictors at multiple spatial scales. Here,...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13089

    authors: Winiarski KJ,Peterman WE,Whiteley AR,McGarigal K

    更新日期:2020-01-01 00:00:00

  • New approaches to species delimitation and population structure of anthozoans: Two case studies of octocorals using ultraconserved elements and exons.

    abstract::As coral populations decline worldwide in the face of ongoing environmental change, documenting their distribution, diversity and conservation status is now more imperative than ever. Accurate delimitation and identification of species is a critical first step. This task, however, is not trivial as morphological varia...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13241

    authors: Erickson KL,Pentico A,Quattrini AM,McFadden CS

    更新日期:2021-01-01 00:00:00

  • Impact of sequence processing and taxonomic classification approaches on eukaryotic community structure from environmental samples with emphasis on diatoms.

    abstract::Next-generation sequencing is a common method for analysing microbial community diversity and composition. Configuring an appropriate sequence processing strategy within the variety of tools and methods is a nontrivial task and can considerably influence the resulting community characteristics. We analysed the V4 regi...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12726

    authors: Hardge K,Neuhaus S,Kilias ES,Wolf C,Metfies K,Frickenhaus S

    更新日期:2018-03-01 00:00:00

  • De novo characterization of the Timema cristinae transcriptome facilitates marker discovery and inference of genetic divergence.

    abstract::Adaptation to different ecological environments can promote speciation. Although numerous examples of such 'ecological speciation' now exist, the genomic basis of the process, and the role of gene flow in it, remains less understood. This is, at least in part, because systems that are well characterized in terms of th...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2012.03121.x

    authors: Comeault AA,Sommers M,Schwander T,Buerkle CA,Farkas TE,Nosil P,Parchman TL

    更新日期:2012-05-01 00:00:00

  • DNA barcode accumulation curves for understudied taxa and areas.

    abstract::Frequently, the diversity of umbrella taxa is invoked to predict patterns of other, less well-known, life. However, the utility of this strategy has been questioned. We tested whether a phylogenetic diversity (PD) analysis of CO1 DNA barcodes could act as a proxy for standard methods of determining sampling efficiency...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02646.x

    authors: Smith MA,Fernandez-Triana J,Roughley R,Hebert PD

    更新日期:2009-05-01 00:00:00

  • angsd-wrapper: utilities for analysing next-generation sequencing data.

    abstract::High-throughput sequencing has changed many aspects of population genetics, molecular ecology and related fields, affecting both experimental design and data analysis. The software package angsd allows users to perform a number of population genetic analyses on high-throughput sequencing data. angsd uses probabilistic...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12578

    authors: Durvasula A,Hoffman PJ,Kent TV,Liu C,Kono TJ,Morrell PL,Ross-Ibarra J

    更新日期:2016-11-01 00:00:00

  • A microarray system for Y chromosomal and mitochondrial single nucleotide polymorphism analysis in chimpanzee populations.

    abstract::Chimpanzee populations are diminishing as a consequence of human activities, and as a result this species is now endangered. In the context of conservation programmes, genetic data can add vital information, for instance on the genetic diversity and structure of threatened populations. Single nucleotide polymorphisms ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.02000.x

    authors: Andrés O,Rönn AC,Bonhomme M,Kellermann T,Crouau-Roy B,Doxiadis G,Verschoor EJ,Goossens B,Domingo-Roura X,Bruford MW,Bosch M,Syvänen AC

    更新日期:2008-05-01 00:00:00