Abstract:
:Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Baird RE,Wadl PA,Wang X,Johnson DH,Rinehart TA,Abbas HK,Shier T,Trigiano RNdoi
10.1111/j.1755-0998.2008.02511.xsubject
Has Abstractpub_date
2009-05-01 00:00:00pages
946-8issue
3eissn
1755-098Xissn
1755-0998journal_volume
9pub_type
杂志文章abstract::Palaeoenvironments and former climates are typically inferred from pollen and macrofossil records. This approach is time-consuming and suffers from low taxonomic resolution and biased taxon sampling. Here, we test an alternative DNA-based approach utilizing the P6 loop in the chloroplast trnL (UAA) intron; a short (13...
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
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pub_type: 杂志文章
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
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