Cross-species amplification of Eucalyptus microsatellite loci.

abstract：:DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR o...

journal_title：Molecular ecology resources

authors： Dittrich-Schröder G,Wingfield MJ,Klein H,Slippers B

更新日期：2012-01-01 00:00:00

abstract：:Degraded DNA from suboptimal field sampling is common in molecular ecology. However, its impact on techniques that use restriction site associated next-generation DNA sequencing (RADSeq, GBS) is unknown. We experimentally examined the effects of in situDNA degradation on data generation for a modified double-digest RA...

journal_title：Molecular ecology resources

authors： Graham CF,Glenn TC,McArthur AG,Boreham DR,Kieran T,Lance S,Manzon RG,Martino JA,Pierson T,Rogers SM,Wilson JY,Somers CM

更新日期：2015-11-01 00:00:00

abstract：:DNA barcoding is a molecular method that rapidly identifies an individual to a known taxon or its closest relative based on a 650-bp fragment of the cytochrome c oxidase subunit I (COI). In this study, DNA barcodes of members of the family Accipitridae, including Haliastur indus (brahminy kite), Haliaeetus leucogaster...

journal_title：Molecular ecology resources

authors： Ong PS,Luczon AU,Quilang JP,Sumaya AM,Ibañez JC,Salvador DJ,Fontanilla IK

更新日期：2011-03-01 00:00:00

abstract：:Microsatellite markers have played a major role in ecological, evolutionary and conservation research during the past 20 years. However, technical constrains related to the use of capillary electrophoresis and a recent technological revolution that has impacted other marker types have brought to question the continued...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Lobréaux S,Quenette PY,Swenson JE,Taberlet P

更新日期：2017-05-01 00:00:00

abstract：:Metabarcoding is increasingly used to assess species diversity by high-throughput sequencing where millions of sequences can be generated in parallel and multiple samples can be analysed in one sequencing run. Generating amplified fragments with a unique sequence identifier ('tag') for each sample is crucial, as it al...

journal_title：Molecular ecology resources

authors： Zizka VMA,Elbrecht V,Macher JN,Leese F

更新日期：2019-07-01 00:00:00

abstract：:An enriched genomic library was constructed and 9 novel polymorphic tetranucleotide microsatellite markers developed for Austrocedrus chilensis, the most economically important native conifer in the Andean Patagonian region. Polymorphism was investigated for these markers in 48 individuals from two populations. Number...

journal_title：Molecular ecology resources

authors： Arana MV,Buonamici A,Sebastiani F,Alia R,Gallo LA,Marchelli P,Moreno C,Vendramin GG

更新日期：2008-07-01 00:00:00

abstract：:Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today's Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-m...

journal_title：Molecular ecology resources

authors： Janzen DH,Hallwachs W,Blandin P,Burns JM,Cadiou JM,Chacon I,Dapkey T,Deans AR,Epstein ME,Espinoza B,Franclemont JG,Haber WA,Hajibabaei M,Hall JP,Hebert PD,Gauld ID,Harvey DJ,Hausmann A,Kitching IJ,Lafontaine D,Lan

更新日期：2009-05-01 00:00:00

abstract：:We present 25 polymorphic microsatellite loci for Intsia palembanica, a highly valued timber species in the Indo-Malayan region, Australia and western Pacific islands. Microsatellite loci were tested for polymorphism across a total of 76 individuals from three natural populations from Irian Jaya, Indonesia. The averag...

journal_title：Molecular ecology resources

authors： Wong KN,Tan WL,Chew FT

更新日期：2009-01-01 00:00:00

abstract：:Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...

journal_title：Molecular ecology resources

authors： Rodríguez-Ezpeleta N,Bradbury IR,Mendibil I,Álvarez P,Cotano U,Irigoien X

更新日期：2016-07-01 00:00:00

abstract：:We present the leaf and floral transcriptomes of two hybridizing bromeliad species that differ in their major pollinator systems. Here we identified candidate genes responsible for pollinator attraction and reproductive isolation in these two species. We searched for candidate genes involved in floral traits, such as ...

journal_title：Molecular ecology resources

authors： Palma-Silva C,Ferro M,Bacci M,Turchetto-Zolet AC

更新日期：2016-07-01 00:00:00

abstract：:We describe a convenient, cost-effective and flexible medium-throughput single nucleotide polymorphism (SNP) genotyping method, Multiplex SNP-SCALE, which enables the simultaneous amplification by polymerase chain reaction (PCR) of up to 25 (or potentially more) loci followed by electrophoresis in an automated DNA seq...

journal_title：Molecular ecology resources

authors： Kenta T,Gratten J,Haigh NS,Hinten GN,Slate J,Butlin RK,Burke T

更新日期：2008-11-01 00:00:00

abstract：:The Chilean isopod Excirolana hirsuticauda is a marine benthic brooder with wide distributional range and low potential for long-distance dispersal. Eleven microsatellite markers were developed for E. hirsuticauda using enriched libraries. Characterization of those loci in 35 individuals from Playa Blanca beach showed...

journal_title：Molecular ecology resources

authors： Haye PA,Marchant S

更新日期：2008-09-01 00:00:00

abstract：:Ecological understanding of the role of consumer-resource interactions in natural food webs is limited by the difficulty of accurately and efficiently determining the complex variety of food types animals have eaten in the field. We developed a method based on DNA metabarcoding multiplexing and next-generation sequenc...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Boyer F,Mercier C,Rioux D,Coissac E,Taberlet P

更新日期：2014-03-01 00:00:00

abstract：:The pygmy grasshopper Tetrix japonica is a common insect distributed throughout the world, and it has the potential for use in studies of body colour polymorphism, genomics and the biology of Tetrigoidea (Insecta: Orthoptera). However, limited biological information is available for this insect. Here, we conducted a d...

journal_title：Molecular ecology resources

authors： Qiu Z,Liu F,Lu H,Huang Y

更新日期：2017-05-01 00:00:00

abstract：:A microsatellite-enriched genomic library was obtained for the endangered Japanese loach Leptobotia curta, and 39 dinucleotide markers were successfully isolated and characterized. These markers had between one and nine alleles, with expected heterozygosity ranging from 0 to 0.839, in a population from the Lake Biwa-Y...

journal_title：Molecular ecology resources

authors： Watanabe K,Takeshima H,Iwata A,Abe T,Uehara K,Kakioka R,Kihira D,Nishida M

更新日期：2008-01-01 00:00:00

abstract：:Stylosanthes capitata is an important tropical pasture legume. Knowledge of genetic diversity and structure of S. capitata populations is of great importance for the conservation and germplasm management of this species. Thus, eight microsatellite markers were developed from an S. capitata-enriched library. They were ...

journal_title：Molecular ecology resources

authors： Santos MO,Sassaki RP,Chiari L,Resende RM,DE Souza AP

更新日期：2009-01-01 00:00:00

abstract：:Rhizoprionodon lalandii and R. porosus are widely distributed along the Atlantic coast of the Americas, living close to coastal areas and therefore frequently captured by seaboard fisheries. However, morphological identification of species in this genus is very difficult, especially when sharks have their heads and fi...

journal_title：Molecular ecology resources

authors： Mendonça FF,Hashimoto DT,Porto-Foresti F,Oliveira C,Gadig OB,Foresti F

更新日期：2009-05-01 00:00:00

abstract：:DNA-based methods have greatly enhanced the sensitivity and specificity of hematophagous arthropod bloodmeal identification. A variety of methods have been applied to study the blood-feeding behaviour of mosquitoes, ticks, black flies and other blood-feeding arthropods as it relates to host-parasite interactions and p...

journal_title：Molecular ecology resources

authors： Kent RJ

更新日期：2009-01-01 00:00:00

abstract：:Type specimens have high scientific importance because they provide the only certain connection between the application of a Linnean name and a physical specimen. Many other individuals may have been identified as a particular species, but their linkage to the taxon concept is inferential. Because type specimens are o...

journal_title：Molecular ecology resources

authors： Prosser SW,deWaard JR,Miller SE,Hebert PD

更新日期：2016-03-01 00:00:00

abstract：:Improving the realism of spatially explicit demographic models is important for better inferring the history of past populations and for understanding the genetic bases of adaptation and speciation. One particular type of demographic event to take into account is long-distance dispersal (LDD). The goals of this study ...

journal_title：Molecular ecology resources

authors： Ray N,Excoffier L

更新日期：2010-09-01 00:00:00

abstract：:Although nematodes are one of the most diverse metazoan phyla, species identification through morphology is difficult. Several genetic markers have been used for their identification, but most do not provide species-level resolution in all groups, and those that do lack primer sets effective across the phylum, preclud...

journal_title：Molecular ecology resources

authors： Prosser SW,Velarde-Aguilar MG,León-Règagnon V,Hebert PD

更新日期：2013-11-01 00:00:00

abstract：:We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

journal_title：Molecular ecology resources

authors： Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

更新日期：2008-01-01 00:00:00

abstract：:DNA microarrays are a popular technique for the detection of microorganisms. Several approaches using specific oligomers targeting one or a few marker genes for each species have been proposed. Data analysis is usually limited to call a species present when its oligomer exceeds a certain intensity threshold. While thi...

journal_title：Molecular ecology resources

authors： Engelmann JC,Rahmann S,Wolf M,Schultz J,Fritzilas E,Kneitz S,Dandekar T,Müller T

更新日期：2009-01-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) has formed the backbone of phylogeographic research for many years; however, recent trends focus on genome-wide analyses. One method proposed for calibrating inferences from noisy next-generation data, such as RAD sequencing, is to compare these results with analyses of mitochondrial sequence...

journal_title：Molecular ecology resources

authors： Stobie CS,Cunningham MJ,Oosthuizen CJ,Bloomer P

更新日期：2019-01-01 00:00:00

abstract：:A significant portion of plant species are polyploids, with ploidy levels sometimes varying among individuals and/or populations. Current techniques to determine the individual ploidy, e.g., flow cytometry, chromosome counting or genotyping-by-sequencing, are often cumbersome. Based on the genotypic probabilities for ...

journal_title：Molecular ecology resources

authors： Huang K,Dunn DW,Li Z,Zhang P,Dai Y,Li B

更新日期：2019-09-01 00:00:00

abstract：:Luffa spp. (sponge gourd or ridge gourd) is an economically important vegetable crop widely cultivated in China, India and Southeast Asia. Here, we employed PacBio long-read single-molecule real-time (SMRT) sequencing to perform de novo genome assemblies of two commonly cultivated Luffa species, L. acutangula and L. c...

journal_title：Molecular ecology resources

authors： Pootakham W,Sonthirod C,Naktang C,Nawae W,Yoocha T,Kongkachana W,Sangsrakru D,Jomchai N,U-Thoomporn S,Sheedy JR,Buaboocha J,Mekiyanon S,Tangphatsornruang S

更新日期：2021-01-01 00:00:00

abstract：:Genetic stock identification (GSI) is an important tool in fisheries management. Microsatellites (μSATs) have been the dominant genetic marker for GSI; however, increasing availability and numerous advantages of single-nucleotide polymorphism (SNP) markers make them an appealing alternative. We tested performance of 1...

journal_title：Molecular ecology resources

authors： Hess JE,Matala AP,Narum SR

更新日期：2011-03-01 00:00:00

abstract：:Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of COI amplification has been quite variable when it comes to freshwater zooplankton (Elías-Gutiérrez & Valdez-Moreno 2008; Jeffery et al. 2011). Some genera of microcrustaceans seem to be more dif...

journal_title：Molecular ecology resources

authors： Prosser S,Martínez-Arce A,Elías-Gutiérrez M

更新日期：2013-11-01 00:00:00

abstract：:Eleven microsatellites were characterized for Semicossyphus pulcher (California sheephead) using an enrichment protocol. The number of alleles varied from three to 14 for a sample of 40 individuals from two populations. Expected heterozygosities ranged from 0.311 to 0.891. All loci but one were in Hardy-Weinberg equil...

journal_title：Molecular ecology resources

authors： Poortvliet M,Olsen JL,Selkoe KA,Coyer JA,Bernardi G

更新日期：2009-01-01 00:00:00

abstract：:sam is a Windows program designed to detect candidate loci for selection in whole-genome scans. It also gives valuable clues as regards the ecological factors at stake in the selection process. The method used is based on multiple univariate logistic regression models to test for association between allelic frequencie...

journal_title：Molecular ecology resources

authors： Joost S,Kalbermatten M,Bonin A

更新日期：2008-09-01 00:00:00