Advancing nematode barcoding: a primer cocktail for the cytochrome c oxidase subunit I gene from vertebrate parasitic nematodes.

Abstract:

:Although nematodes are one of the most diverse metazoan phyla, species identification through morphology is difficult. Several genetic markers have been used for their identification, but most do not provide species-level resolution in all groups, and those that do lack primer sets effective across the phylum, precluding high-throughput processing. This study describes a cocktail of three novel primer pairs that overcome this limitation by recovering cytochrome c oxidase I (COI) barcodes from diverse nematode lineages parasitic on vertebrates, including members of three orders and eight families. Its effectiveness across a broad range of nematodes enables high-throughput processing.

journal_name

Mol Ecol Resour

authors

Prosser SW,Velarde-Aguilar MG,León-Règagnon V,Hebert PD

doi

10.1111/1755-0998.12082

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

1108-15

issue

6

eissn

1755-098X

issn

1755-0998

journal_volume

13

pub_type

杂志文章
  • Multiscale resistant kernel surfaces derived from inferred gene flow: An application with vernal pool breeding salamanders.

    abstract::The importance of assessing spatial data at multiple scales when modelling species-environment relationships has been highlighted by several empirical studies. However, no landscape genetics studies have optimized landscape resistance surfaces by evaluating relevant spatial predictors at multiple spatial scales. Here,...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13089

    authors: Winiarski KJ,Peterman WE,Whiteley AR,McGarigal K

    更新日期:2020-01-01 00:00:00

  • Comparison of SNPs and microsatellites for fine-scale application of genetic stock identification of Chinook salmon in the Columbia River Basin.

    abstract::Genetic stock identification (GSI) is an important tool in fisheries management. Microsatellites (μSATs) have been the dominant genetic marker for GSI; however, increasing availability and numerous advantages of single-nucleotide polymorphism (SNP) markers make them an appealing alternative. We tested performance of 1...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02958.x

    authors: Hess JE,Matala AP,Narum SR

    更新日期:2011-03-01 00:00:00

  • Genome-wide cross-amplification of domestic sheep microsatellites in bighorn sheep and mountain goats.

    abstract::We tested for cross-species amplification of microsatellite loci located throughout the domestic sheep (Ovis aries) genome in two north American mountain ungulates (bighorn sheep, Ovis canadensis, and mountain goats, Oreamnos americanus). We identified 247 new polymorphic markers in bighorn sheep (≥ 3 alleles in one o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02575.x

    authors: Poissant J,Shafer AB,Davis CS,Mainguy J,Hogg JT,Côté SD,Coltman DW

    更新日期:2009-07-01 00:00:00

  • DNA barcoding in native plants of the Labiatae (Lamiaceae) family from Chios Island (Greece) and the adjacent Çeşme-Karaburun Peninsula (Turkey).

    abstract::The plant family Labiatae (Lamiaceae) is known for its fine medicinal and aromatic herbs like lavender, mint, oregano, sage and thyme and is a rich source of essential oils for the food, pharmaceutical and cosmetic industry. Besides its great economic importance, the Labiatae family contributes significantly to the en...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2012.03129.x

    authors: Theodoridis S,Stefanaki A,Tezcan M,Aki C,Kokkini S,Vlachonasios KE

    更新日期:2012-07-01 00:00:00

  • The potential of distance-based thresholds and character-based DNA barcoding for defining problematic taxonomic entities by CO1 and ND1.

    abstract::The mitochondrial CO1 gene (cytochrome c oxidase I) is a widely accepted metazoan barcode region. In insects, the mitochondrial NADH dehydrogenase subunit 1 (ND1) gene region has proved to be another suitable marker especially for the identification of lower level taxonomic entities such as populations and sister spec...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12125

    authors: Bergmann T,Rach J,Damm S,Desalle R,Schierwater B,Hadrys H

    更新日期:2013-11-01 00:00:00

  • Isolation and characterization of microsatellite markers from the white-ruffed manakin Corapipo altera (Aves, Pipridae).

    abstract::We describe 15 polymorphic microsatellite loci from the white-ruffed manakin Corapipo altera, a common understory bird of Neotropical lowland and montane evergreen forests from eastern Honduras to northwestern Colombia. These markers were developed in order to assess population structure and genetic diversity in a fra...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.01929.x

    authors: Barnett JR,Stenzler LM,Ruiz-Gutierrez V,Bogdanowicz SM,Lovette IJ

    更新日期:2008-01-01 00:00:00

  • Genomic resources notes accepted 1 April 2013-31 May 2013.

    abstract::This article documents the public availability of approximately 15 000 polymorphic SNP loci for the bighorn sheep Ovis canadensis. ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12142

    authors: Genomic Resources Development Consortium.,Coltman DW,Hogg JT,Miller JM

    更新日期:2013-09-01 00:00:00

  • Predicting polymorphic EST-SSRs in silico.

    abstract::The public availability of large quantities of gene sequence data provides a valuable resource of the mining of Simple Sequence Repeat (SSR) molecular genetic markers for genetic analysis. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated gene...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12078

    authors: Duran C,Singhania R,Raman H,Batley J,Edwards D

    更新日期:2013-05-01 00:00:00

  • Evaluation of primer pairs for microbiome profiling from soils to humans within the One Health framework.

    abstract::The 'One Health' framework emphasizes the ecological relationships between soil, plant, animal and human health. Microbiomes play important roles in these relationships, as they modify the health and performance of the different compartments and influence the transfer of energy, matter and chemicals between them. Stan...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13215

    authors: Wasimuddin,Schlaeppi K,Ronchi F,Leib SL,Erb M,Ramette A

    更新日期:2020-11-01 00:00:00

  • Untangling ITS2 genotypes of algal symbionts in zooxanthellate corals.

    abstract::Collectively called zooxanthellae, photosynthetic dinoflagellates in the family Symbiodiniaceae are typical endosymbionts that unequivocally mediate coral responses to environmental changes. Symbiodiniaceae are genetically diverse, encompassing at least nine phylogenetically distinct genera (clades A-I). The ribosomal...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13250

    authors: Shi T,Niu G,Kvitt H,Zheng X,Qin Q,Sun D,Ji Z,Tchernov D

    更新日期:2021-01-01 00:00:00

  • Identification and characterization of microsatellite loci in Intsia palembanica (Leguminosae), a valuable tropical timber species.

    abstract::We present 25 polymorphic microsatellite loci for Intsia palembanica, a highly valued timber species in the Indo-Malayan region, Australia and western Pacific islands. Microsatellite loci were tested for polymorphism across a total of 76 individuals from three natural populations from Irian Jaya, Indonesia. The averag...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02491.x

    authors: Wong KN,Tan WL,Chew FT

    更新日期:2009-01-01 00:00:00

  • Development of 54 novel single-nucleotide polymorphism (SNP) assays for sockeye and coho salmon and assessment of available SNPs to differentiate stocks within the Columbia River.

    abstract::Single-nucleotide polymorphisms (SNPs) have potential for broad application in population and conservation genetics, but availability of these markers is limited in many nonmodel species. In this study, genomic and expressed sequence tagged (EST) sequences from closely related salmonids (Chinook salmon and rainbow tro...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.02977.x

    authors: Campbell NR,Narum SR

    更新日期:2011-03-01 00:00:00

  • Ten microsatellite loci from Solms-laubachia eurycarpa (Brassicaceae).

    abstract::Solms-laubachia eurycarpa is a medicinal herb endemic to the Hengduan Mountains region of south-central China. We screened a partial genomic library enriched for microsatellites and characterized 10 polymorphic loci for S. eurycarpa. The number of alleles per locus ranged from five to 15, with an average of 9.6. The o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02141.x

    authors: Yue J,Feldheim KA,Sun H,Ree R

    更新日期:2008-09-01 00:00:00

  • Genomic sequence capture of haemosporidian parasites: Methods and prospects for enhanced study of host-parasite evolution.

    abstract::Avian malaria and related haemosporidians (Plasmodium, [Para]Haemoproteus and Leucocytoozoon) represent an exciting multihost, multiparasite system in ecology and evolution. Global research in this field accelerated after the publication in 2000 of PCR protocols to sequence a haemosporidian mitochondrial (mtDNA) barco...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12977

    authors: Barrow LN,Allen JM,Huang X,Bensch S,Witt CC

    更新日期:2019-03-01 00:00:00

  • VIP Barcoding: composition vector-based software for rapid species identification based on DNA barcoding.

    abstract::Species identification based on short sequences of DNA markers, that is, DNA barcoding, has emerged as an integral part of modern taxonomy. However, software for the analysis of large and multilocus barcoding data sets is scarce. The Basic Local Alignment Search Tool (BLAST) is currently the fastest tool capable of ha...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12235

    authors: Fan L,Hui JH,Yu ZG,Chu KH

    更新日期:2014-07-01 00:00:00

  • Characterization and analysis of a de novo transcriptome from the pygmy grasshopper Tetrix japonica.

    abstract::The pygmy grasshopper Tetrix japonica is a common insect distributed throughout the world, and it has the potential for use in studies of body colour polymorphism, genomics and the biology of Tetrigoidea (Insecta: Orthoptera). However, limited biological information is available for this insect. Here, we conducted a d...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12553

    authors: Qiu Z,Liu F,Lu H,Huang Y

    更新日期:2017-05-01 00:00:00

  • Developing a series of conservative anchor markers and their application to phylogenomics of Laurasiatherian mammals.

    abstract::The availability of numerous universal markers and suitable phylogenetic analysis methods are both very important for phylogenomics inference. Based on PCR amplification, a total of 122 markers, which were amplified in 19 representative species, were developed for Laurasiatherian phylogenomics. Subsequently, we illust...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02903.x

    authors: Zhou X,Xu S,Zhang P,Yang G

    更新日期:2011-01-01 00:00:00

  • PERMANENT GENETIC RESOURCES: Isolation of 60 polymorphic microsatellite loci in EST libraries of four sibling species of the phytopathogenic fungal complex Microbotryum.

    abstract::We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum, causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis-dioicae, M. violaceum sensus stricto, M. lagerheimii and M....

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.01967.x

    authors: Giraud T,Yockteng R,Marthey S,Chiapello H,Jonot O,Lopez-Villavicencio M,DE Vienne DM,Hood ME,Refregier G,Gendrault-Jacquemard A,Wincker P,Dossat C

    更新日期:2008-03-01 00:00:00

  • Chromosome-level genome assembly of the coastal horseshoe crab (Tachypleus gigas).

    abstract::Horseshoe crabs, represented by only four extant species, have existed for around 500 million years. However, their existence is now under threat because of anthropogenic activities. The availability of genomic resources for these species will be valuable in planning appropriate conservation measures. Whole-genome seq...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13233

    authors: Shingate P,Ravi V,Prasad A,Tay BH,Venkatesh B

    更新日期:2020-11-01 00:00:00

  • Eighteen new tetranucleotide microsatellite DNA markers for Coregonus lavaretus cloned from an alpine lake population.

    abstract::We developed 18 polymorphic microsatellite markers for Coregonus lavaretus from genomic libraries enriched for (GACA)(n) and (GATA)(n) repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats. These loci were screened in 69 individuals from two alpine populations in Austria. Allelic vari...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02153.x

    authors: Winkler KA,Weiss S

    更新日期:2008-09-01 00:00:00

  • TECHNICAL ADVANCES: Effects of genotyping protocols on success and errors in identifying individual river otters (Lontra canadensis) from their faeces.

    abstract::In noninvasive genetic sampling, when genotyping error rates are high and recapture rates are low, misidentification of individuals can lead to overestimation of population size. Thus, estimating genotyping errors is imperative. Nonetheless, conducting multiple polymerase chain reactions (PCRs) at multiple loci is tim...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.01992.x

    authors: Hansen H,Ben-David M,McDonald DB

    更新日期:2008-03-01 00:00:00

  • PERMANENT GENETIC RESOURCES: Consensus primers of cyp73 genes discriminate willow species and hybrids (Salix, Salicaceae).

    abstract::Consensus primers, based on exon sequences of the cyp73 gene family coding for cinnamate 4-hydroxylase (C4H) of the lignin biosynthesis pathway, were designed for the tetraploid willow species Salix alba and Salix fragilis. Diagnostic alleles at species level were observed among introns of three cyp73 genes and allowe...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.01991.x

    authors: Trung le Q,VAN Puyvelde K,Triest L

    更新日期:2008-03-01 00:00:00

  • Population structure of Atlantic mackerel inferred from RAD-seq-derived SNP markers: effects of sequence clustering parameters and hierarchical SNP selection.

    abstract::Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12518

    authors: Rodríguez-Ezpeleta N,Bradbury IR,Mendibil I,Álvarez P,Cotano U,Irigoien X

    更新日期:2016-07-01 00:00:00

  • A combined parasitological molecular approach for noninvasive characterization of parasitic nematode communities in wild hosts.

    abstract::Most hosts are concurrently or sequentially infected with multiple parasites; thus, fully understanding interactions between individual parasite species and their hosts depends on accurate characterization of the parasite community. For parasitic nematodes, noninvasive methods for obtaining quantitative, species-speci...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12382

    authors: Budischak SA,Hoberg EP,Abrams A,Jolles AE,Ezenwa VO

    更新日期:2015-09-01 00:00:00

  • Multiplex pre-amplification for noninvasive genetic sampling: is the extra effort worth it?

    abstract::Microsatellite genotyping of hair and faeces using standard polymerase chain reaction (PCR) resulted in low success rates and high error rates in a 2003-2004 pilot study using noninvasive genetic sampling for the brown bear (Ursus arctos) in the Italian Alps. Thus, we evaluated the performance of multiplex pre-amplifi...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02818.x

    authors: DE Barba M,Waits LP

    更新日期:2010-07-01 00:00:00

  • The chromosome-level genome sequence and karyotypic evolution of Megadenia pygmaea (Brassicaceae).

    abstract::Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was a...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13291

    authors: Yang W,Zhang L,Mandáková T,Huang L,Li T,Jiang J,Yang Y,Lysak MA,Liu J,Hu Q

    更新日期:2020-11-05 00:00:00

  • Spatial analysis method (sam): a software tool combining molecular and environmental data to identify candidate loci for selection.

    abstract::sam is a Windows program designed to detect candidate loci for selection in whole-genome scans. It also gives valuable clues as regards the ecological factors at stake in the selection process. The method used is based on multiple univariate logistic regression models to test for association between allelic frequencie...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02162.x

    authors: Joost S,Kalbermatten M,Bonin A

    更新日期:2008-09-01 00:00:00

  • Characterization of the transcriptome and gene expression of four different tissues in the ecologically relevant sea urchin Arbacia lixula using RNA-seq.

    abstract::The sea urchin Arbacia lixula is a keystone species in Mediterranean ecosystems that drive landscape changes in littoral communities. However, genomic information available for the whole order Arbacioida is very limited. Using RNA-seq techniques, we have characterized the transcriptome of four different tissue types i...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12500

    authors: Pérez-Portela R,Turon X,Riesgo A

    更新日期:2016-05-01 00:00:00

  • A microarray system for Y chromosomal and mitochondrial single nucleotide polymorphism analysis in chimpanzee populations.

    abstract::Chimpanzee populations are diminishing as a consequence of human activities, and as a result this species is now endangered. In the context of conservation programmes, genetic data can add vital information, for instance on the genetic diversity and structure of threatened populations. Single nucleotide polymorphisms ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.02000.x

    authors: Andrés O,Rönn AC,Bonhomme M,Kellermann T,Crouau-Roy B,Doxiadis G,Verschoor EJ,Goossens B,Domingo-Roura X,Bruford MW,Bosch M,Syvänen AC

    更新日期:2008-05-01 00:00:00

  • neogen: A tool to predict genetic effective population size (Ne ) for species with generational overlap and to assist empirical Ne study design.

    abstract::Molecular genetic estimates of population effective size (Ne ) lose accuracy and precision when insufficient numbers of samples or loci are used. Ideally, researchers would like to forecast the necessary power when planning their project. neogen (genetic Ne for Overlapping Generations) enables estimates of precision a...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12941

    authors: Blower DC,Riginos C,Ovenden JR

    更新日期:2019-01-01 00:00:00