TECHNICAL ADVANCES: Effects of genotyping protocols on success and errors in identifying individual river otters (Lontra canadensis) from their faeces.

abstract：:We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cr...

journal_title：Molecular ecology resources

authors： Pelz-Serrano K,Munguia-Vega A,Piaggio AJ,Neubaum M,Munclinger P,Pártl A,VAN Riper Iii C,Culver M

更新日期：2009-03-01 00:00:00

abstract：:As coral populations decline worldwide in the face of ongoing environmental change, documenting their distribution, diversity and conservation status is now more imperative than ever. Accurate delimitation and identification of species is a critical first step. This task, however, is not trivial as morphological varia...

journal_title：Molecular ecology resources

authors： Erickson KL,Pentico A,Quattrini AM,McFadden CS

更新日期：2021-01-01 00:00:00

abstract：:Sex-linked molecular markers have become valuable tools for understanding sex ratio evolution and sex-specific physiology in pre-reproductive plants. To develop new accurate methods for sexing Distichlis spicata juveniles and nonflowering individuals, we converted a random amplified polymorphic DNA-polymerase chain re...

journal_title：Molecular ecology resources

authors： Eppley SM,O'Quinn R,Brown AL

更新日期：2009-09-01 00:00:00

abstract：:Rhinobatos percellens, Rhinobatos horkelli and Zapteryx brevirostris, known as guitarfish, are commonly found inshore and caught by trawl nets. Recently, these species have been suffering sharp population declines. One of the main impediments to the development of conservation plans is the lack of information related ...

journal_title：Molecular ecology resources

authors： DE-Franco B,Mendonça FF,Hashimoto DT,Porto-Foresti F,Oliveira C,Foresti F

更新日期：2010-01-01 00:00:00

abstract：:Many plants and some animal species are polyploids. Nondisomically inherited markers (e.g. microsatellites) in such species cannot be analysed directly by standard population genetics methods developed for diploid species. One solution is to transform the polyploid codominant genotypes to pseudodiploid-dominant genoty...

journal_title：Molecular ecology resources

authors： Wang J,Scribner KT

更新日期：2014-05-01 00:00:00

abstract：:To characterize microbiomes and other ecological assemblages, ecologists routinely sequence and compare loci that differ among focal taxa. Counts of these sequences convey information regarding the occurrence and relative abundances of taxa, but provide no direct measure of their absolute abundances, due to the techni...

journal_title：Molecular ecology resources

authors： Harrison JG,John Calder W,Shuman B,Alex Buerkle C

更新日期：2021-01-01 00:00:00

abstract：:We developed a total of 50 microsatellite markers for the three fungal pathogens causing the most important leaf spot diseases of banana: 32 loci for Mycosphaerella fijiensis are presented, and nine loci each for Mycosphaerella musicola and Mycosphaerella eumusae. All these loci were polymorphic within each species on...

journal_title：Molecular ecology resources

authors： Zapater MF,Duchemin M,Dussart JF,Coste D,Brottier P,Carlier J

更新日期：2008-09-01 00:00:00

abstract：:The rice leaffolder, Cnaphalocrocis medinalis Guenée (Crambidae, Lepidoptera), is an important agricultural pest that causes serious losses to rice production in rice-growing regions with high humidity and temperature. However, a lack of genomic resources limits in-depth understanding of its biological characteristics...

journal_title：Molecular ecology resources

authors： Zhao X,Xu H,He K,Shi Z,Chen X,Ye X,Mei Y,Yang Y,Li M,Gao L,Xu L,Xiao H,Liu Y,Lu Z,Li F

更新日期：2021-02-01 00:00:00

abstract：:This article documents the public availability of approximately 15 000 polymorphic SNP loci for the bighorn sheep Ovis canadensis. ...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Coltman DW,Hogg JT,Miller JM

更新日期：2013-09-01 00:00:00

abstract：:With striking morphological diversity and adaptability, Chamaedorea palms constitute an ecologically and economically important understorey component of Neotropical forests. Nine loci developed for Chamaedorea elegans evaluated in three Veracruz populations resulted in a large number of alleles (8-18), and high expect...

journal_title：Molecular ecology resources

authors： Cibrián-Jaramillo A,Hahn WJ,Desalle R

更新日期：2008-03-01 00:00:00

abstract：:This study reports the isolation and characterization of 11 polymorphic microsatellites from a sand smelt (Atherina boyeri) genomic library. Enrichment was performed with di-, tri- and tetranucleotide motifs following the FIASCO procedure (fast isolation by AFLP of sequences containing repeats). All loci were found to...

journal_title：Molecular ecology resources

authors： Milana V,Sola L,Rossi AR,Barbisan F,Congiu L

更新日期：2009-05-01 00:00:00

abstract：:Stegodyphus lineatus spiders live in groups consisting of closely related individuals. There appears to be no discrimination against related individuals as mates but females mate multiply, despite the fact that matings are shown to carry a cost. We have developed eight polymorphic dinucleotide microsatellite markers t...

journal_title：Molecular ecology resources

authors： Bilde T,Tuni C,Cariani A,Santini A,Tabarroni C,Garoia F,Goodacre SL

更新日期：2009-01-01 00:00:00

abstract：:New DNA sequencing technologies are allowing researchers to explore the genomes of the millions of natural history specimens collected prior to the molecular era. Yet, we know little about how well specific next-generation sequencing (NGS) techniques work with the degraded DNA typically extracted from museum specimens...

journal_title：Molecular ecology resources

authors： McCormack JE,Tsai WL,Faircloth BC

更新日期：2016-09-01 00:00:00

abstract：:The ability to detect the identity of a sample obtained from its environment is a cornerstone of molecular ecological research. Thanks to the falling price of shotgun sequencing, genome skimming, the acquisition of short reads spread across the genome at low coverage, is emerging as an alternative to traditional barco...

journal_title：Molecular ecology resources

authors： Rachtman E,Balaban M,Bafna V,Mirarab S

更新日期：2020-05-01 00:00:00

abstract：:Microsatellite markers have played a major role in ecological, evolutionary and conservation research during the past 20 years. However, technical constrains related to the use of capillary electrophoresis and a recent technological revolution that has impacted other marker types have brought to question the continued...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Lobréaux S,Quenette PY,Swenson JE,Taberlet P

更新日期：2017-05-01 00:00:00

abstract：:The construction of microsatellite-enriched libraries is an indispensable tool to search for molecular markers as complete genome sequences are still not available for the majority of species of interest. Numerous protocols are available in the literature for the construction of these libraries; however, sometimes the...

journal_title：Molecular ecology resources

authors： Techen N,Arias RS,Glynn NC,Pan Z,Khan IA,Scheffler BE

更新日期：2010-05-01 00:00:00

abstract：:Sequence-based specimen identification, known as DNA barcoding, is a common method complementing traditional morphology-based taxonomic assignments. The fundamental resource in DNA barcoding is the availability of a taxonomically reliable sequence database to use as a reference for sequence comparisons. Here, we provi...

journal_title：Molecular ecology resources

authors： Barco A,Raupach MJ,Laakmann S,Neumann H,Knebelsberger T

更新日期：2016-01-01 00:00:00

abstract：:Eight polymorphic microsatellite loci were isolated and characterized from a microsatellite DNA-enriched DNA library for the leopard coralgrouper (Plectropomus leopardus Lacepède, 1802), a popular food fish in the East Indies. These loci showed polymorphism information content ranging from 0.493 to 0.854, allele numbe...

journal_title：Molecular ecology resources

authors： Ding SX,Zeng HS,Wang Y,Pan Y,Shi XF

更新日期：2009-11-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP) fingerprint data are now commonly collected using DNA sequencers. AFLP genotypes are still often scored by eye from such data - a time-consuming, error-prone and subjective process. We present a semi-automated method of genotyping sequencer-collected AFLPs at predefined fr...

journal_title：Molecular ecology resources

authors： Whitlock R,Hipperson H,Mannarelli M,Butlin RK,Burke T

更新日期：2008-07-01 00:00:00

abstract：:Interpretation of high-throughput sequence data requires an understanding of how decisions made during bioinformatic data processing can influence results. One source of bias that is often cited is PCR clones (or PCR duplicates). PCR clones are common in restriction site-associated sequencing (RAD-seq) data sets, whic...

journal_title：Molecular ecology resources

authors： Euclide PT,McKinney GJ,Bootsma M,Tarsa C,Meek MH,Larson WA

更新日期：2020-01-01 00:00:00

abstract：:Microsatellite markers were developed to assess population structure and patterns of translocation in the introduced European common genet (Genetta genetta). Primer pairs were designed for 60 microsatellite sequences enriched for CA, GA, CATC and TAGA repeat motifs. Eleven loci that proved to be polymorphic were genot...

journal_title：Molecular ecology resources

authors： Gaubert P,Del Cerro I,Palomares F,Godoy JA

更新日期：2008-11-01 00:00:00

abstract：:Phylogenomic methods have proven useful for resolving deep nodes and recalcitrant groups in the spider tree of life. Across arachnids, transcriptomic approaches may generate thousands of loci, and target-capture methods, using the previously designed arachnid-specific probe set, can target a maximum of about 1,000 loc...

journal_title：Molecular ecology resources

authors： Kulkarni S,Wood H,Lloyd M,Hormiga G

更新日期：2020-01-01 00:00:00

abstract：:Thirteen polymorphic microsatellite markers were isolated and characterized from the great hornbill, Buceros bicornis. In analyses of 20 individuals, the numbers of alleles per locus varied from two to 11. The expected and observed heterozygosities ranged from 0.22 to 0.88 and from 0.20 to 1.00, respectively. The mean...

journal_title：Molecular ecology resources

authors： Chamutpong S,Saito DS,Viseshakul N,Nishiumi I,Poonswad P,Ponglikitmongkol M

更新日期：2009-03-01 00:00:00

abstract：:Microbial fitness is easy to measure in the laboratory, but difficult to measure in the field. Laboratory fitness assays make use of controlled conditions and genetically modified organisms, neither of which are available in the field. Among other applications, fitness assays can help researchers detect adaptation to ...

journal_title：Molecular ecology resources

authors： Boynton PJ,Stelkens R,Kowallik V,Greig D

更新日期：2017-05-01 00:00:00

abstract：:In the Alectoris partridges (Phasianidae), hybridization occurs occasionally as a result of the natural breakdown of isolating mechanisms but more frequently as a result of human activity. No genetic record of hybridization is known for the barbary partridge (A. barbara). This species is distributed mostly in North Af...

journal_title：Molecular ecology resources

authors： Barbanera F,Guerrini M,Bertoncini F,Cappelli F,Muzzeddu M,Dini F

更新日期：2011-01-01 00:00:00

abstract：:Incorporating historical tissues into the study of ecological, conservation and management questions can broaden the scope of population genetic research by enhancing our understanding of evolutionary processes and anthropogenic influences on natural populations. Genotyping historical and low-quality samples has been ...

journal_title：Molecular ecology resources

authors： Smith MJ,Pascal CE,Grauvogel Z,Habicht C,Seeb JE,Seeb LW

更新日期：2011-03-01 00:00:00

abstract：:Here, we describe Assemble Species by Automatic Partitioning (ASAP), a new method to build species partitions from single locus sequence alignments (i.e., barcode data sets). ASAP is efficient enough to split data sets as large 104 sequences into putative species in several minutes. Although grounded in evolutionary t...

journal_title：Molecular ecology resources

authors： Puillandre N,Brouillet S,Achaz G

更新日期：2021-02-01 00:00:00

abstract：:A variety of challenges arise when monitoring wildlife populations for disease. Sampling tissues can be invasive to hosts, and obtaining sufficient sample sizes can be expensive and time-consuming, particularly for rare species and when pathogen prevalence is low. Environmental DNA (eDNA)-based detection of pathogens ...

journal_title：Molecular ecology resources

authors： Hall EM,Crespi EJ,Goldberg CS,Brunner JL

更新日期：2016-03-01 00:00:00

abstract：:We report the development of 10 polymorphic molecular markers containing short tandem repeats in the cosmopolitan reef-building coral, Pocillopora damicornis, an important model species for coral health, physiology, ecology, and genetics. The availability of polymorphic DNA markers in P. damicornis can act as impetus ...

journal_title：Molecular ecology resources

authors： Starger CJ,Yeoh SS,Dai CF,Baker AC,Desalle R

更新日期：2008-05-01 00:00:00

abstract：:Luffa spp. (sponge gourd or ridge gourd) is an economically important vegetable crop widely cultivated in China, India and Southeast Asia. Here, we employed PacBio long-read single-molecule real-time (SMRT) sequencing to perform de novo genome assemblies of two commonly cultivated Luffa species, L. acutangula and L. c...

journal_title：Molecular ecology resources

authors： Pootakham W,Sonthirod C,Naktang C,Nawae W,Yoocha T,Kongkachana W,Sangsrakru D,Jomchai N,U-Thoomporn S,Sheedy JR,Buaboocha J,Mekiyanon S,Tangphatsornruang S

更新日期：2021-01-01 00:00:00