Abstract:
:To characterize microbiomes and other ecological assemblages, ecologists routinely sequence and compare loci that differ among focal taxa. Counts of these sequences convey information regarding the occurrence and relative abundances of taxa, but provide no direct measure of their absolute abundances, due to the technical limitations of the sequencing process. The relative abundances in compositional data are inherently constrained and difficult to interpret. The incorporation of internal standards (ISDs; colloquially referred to as 'spike-ins') into DNA pools can ameliorate the problems posed by relative abundance data and allow absolute abundances to be approximated. Unfortunately, many laboratory and sampling biases cause ISDs to underperform or fail. Here, we discuss how careful deployment of ISDs can avoid these complications and be an integral component of well-designed studies seeking to characterize ecological assemblages via sequencing of DNA.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Harrison JG,John Calder W,Shuman B,Alex Buerkle Cdoi
10.1111/1755-0998.13247subject
Has Abstractpub_date
2021-01-01 00:00:00pages
30-43issue
1eissn
1755-098Xissn
1755-0998journal_volume
21pub_type
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